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Gene information
General information
TC002545 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 23758242..23826818
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002545
    iBeetle screen iB_03399

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002545 RA
      MWSALSRTV VTLCRGRQQ KRSPILKND ANSPTMPPP 
      QITAASERQ SFEISSIFL GDQISDYLI SHPDFLERY 
      IMEEVEVEQ LERWIIRKS QRLKKKPES ASRNGRKTS 
      LSRWKFCVH ADKRQMLQD LTHSLQLRP TKDHVLWEL 
      ANCICSAVN ADGFRLYLV DKGDPESLA LFLGIECKN 
      ENGEPKLQK IKSGVSVPL YVAKTLEPV RLSRGEADP 
      RFPDGIPND GDIAHVLCQ ALMQPDGQL AAVLEMWRR 
      EHEGPFYEE DEEIASSYL VWGGIALHY AHLYLNMNK 
      QKKLNDFLL AVVKSIFQD MVSMDMLVM KIMNFAQRL 
      VDADRASLF LVDNKSKEL YATIFDVGV EKDQVECSN 
      GSGDNEEFG KVDTSKEIR FPLGTGIAG QVAMTGEVL 
      NIKDAYADS RFNRTVDQL TGYRTNTIL CMPIFIRGS 
      VIGVVQMVN KHSGLFTIE DEEAFEMFA VYCGLALHH 
      AKLYDKIRK SEQKYRVAL EVLSYHNSC TEEEYQIAV 
      REGIPEKIV GIDDYYFNP FQIEDFHKA IYAMYMFID 
      LFGLARFDK NCLMRFSLT VKKNYRRVP YHNWTHGFS 
      VANSMYSLI KHSAGAFRP NECLALYIG ALCHDLDHR 
      GKNNKFMLD TESPLAAIY STSTMEHHH FNQTVTILQ 
      QEGHNIFAK LSSSEYKQV LSLLKHCIL ATDLAVFFP 
      NKAKLSQLV QSNAFSWNN LEHRLLLQA ISMTGSDLS 
      ASAKPWDIQ VETVKVIFE EFYEQGDAE RQAGRKPIP 
      MMDRHQPDQ QAASQVGFL TGICVSCYS LLYTLIPET 
      KPLLDMCQE NLERWRKID NEIKEKQIQ N
    CDS sequence
    • >TC002545 RA
      atgtggagt gctctttcg cgaactgtt gttactctc 
      tgcagagga agacaacaa aaaagatct ccaatattg 
      aagaatgac gcgaactca ccgaccatg ccaccgcca 
      caaattaca gctgcaagt gaacggcaa agctttgaa 
      atttcttcg atttttcta ggcgatcaa atttctgat 
      tacctcatc agccatccg gatttcctg gagcgctac 
      attatggaa gaggttgaa gtggagcaa ctggaaagg 
      tggatcata cgtaaatct caacgttta aaaaagaaa 
      ccggaaagt gcgagcagg aacggccgg aaaaccagt 
      ctttcgaga tggaagttt tgtgtacac gccgacaaa 
      cgccagatg ctgcaggat ctcactcac agcctacaa 
      ctaaggcct acgaaagat cacgtcctg tgggaattg 
      gccaactgc atttgttct gccgttaat gcagacggg 
      tttaggttg tatctggtg gataaaggt gatcccgaa 
      agtttagcc ctcttttta ggaattgag tgcaagaat 
      gaaaatgga gagcccaag ttacagaaa ataaaaagt 
      ggagtcagc gttccattg tatgttgcc aaaactctt 
      gaacctgtg agactttcc agaggcgag gcagatcct 
      cgttttccg gatggaata ccaaacgat ggggacatc 
      gcccatgtt ttatgtcaa gcgctaatg cagcccgac 
      gggcaatta gccgcagtc ctcgaaatg tggcgtcgc 
      gagcacgag ggccccttc tacgaagag gacgaggaa 
      atcgcatcc agttatcta gtctggggt gggatagct 
      ctgcactac gcccacctt tatctcaac atgaacaag 
      cagaagaag ctcaacgat tttctcctg gctgtggtc 
      aaatcgatt ttccaagat atggtcagc atggacatg 
      ctggtgatg aaaataatg aactttgct caaagacta 
      gtggatgcg gatcgcgcg tccctattt ctggttgac 
      aataagagc aaagaatta tatgccact atatttgat 
      gtgggagtt gagaaagat caagttgaa tgttcgaat 
      ggcagcggt gacaacgag gagtttggg aaagtcgac 
      acgtcaaag gagattcgg tttcctctg gggacggga 
      atcgcaggc caggtggct atgaccggg gaagttctc 
      aacatcaaa gacgcctat gctgattcg aggtttaac 
      cgcacggtc gatcaactt acaggttat cgaaccaac 
      accatcctc tgcatgccc attttcatt cgaggctca 
      gtgatcggc gtggtccag atggtgaac aaacattcc 
      ggcctattt accatcgag gacgaggaa gcgttcgaa 
      atgtttgcc gtttactgt ggactggcc ttacatcac 
      gccaaactt tacgacaaa atccgcaaa tccgagcag 
      aaatataga gtggctctc gaagtatta agttaccac 
      aatagctgt accgaggag gaatatcaa attgcagtg 
      cgcgaggga attccggag aaaattgta ggaatcgac 
      gattactac ttcaatccc ttccaaatc gaagatttc 
      cataaagcc atctacgcg atgtacatg ttcatcgac 
      ctcttcggc ctcgccaga ttcgacaag aattgcctc 
      atgaggttc tcgctcact gtgaaaaag aattacaga 
      agggtgccc tatcataat tggacgcac ggcttctcc 
      gtcgccaac tccatgtac agcctcata aagcattcg 
      gcgggcgct tttcgccca aacgaatgc ctggcattg 
      tacataggg gcactgtgt catgatctc gaccaccgc 
      gggaagaac aacaaattt atgttggac acggaatcg 
      cctctggct gccatctat tccacatcc accatggaa 
      caccaccat ttcaatcaa acggtgacc attttgcaa 
      caggagggc cataatatc tttgccaaa ctctcgagt 
      tctgagtac aaacaagtc ctaagtttg cttaagcac 
      tgcatcctc gccaccgac ttagcagtg ttcttcccc 
      aacaaagct aaactaagc caattggtc caaagcaac 
      gcgttctcc tggaacaac ttagagcac cggttatta 
      ctgcaagcc atttcgatg accggcagt gatctcagt 
      gcgagcgcc aagccgtgg gacatccaa gtcgagacc 
      gttaaagtc atcttcgag gagttttac gagcaagga 
      gatgccgaa agacaagct ggcagaaaa cccattccg 
      atgatggac aggcaccag ccggaccaa caagccgcc 
      tctcaagtg ggctttttg acgggaatt tgtgtttct 
      tgctatagt ttgctttat actttgatt cctgaaact 
      aaaccgtta ttggacatg tgtcaggaa aatttggag 
      cgttggcgg aaaatcgac aatgaaatc aaagaaaaa 
      caaatacaa aactga
    mRNA sequence
    • >TC002545 RA
      catgaatcg aacgcgtcg tgctgctac caaaccgcg 
      atgacatgt ggagtgctc tttcgcgaa ctgttgtta 
      ctctctgca gaggaagac aacaaaaaa gatctccaa 
      tattgaaga atgacgcga actcaccga ccatgccac 
      cgccacaaa ttacagctg caagtgaac ggcaaagct 
      ttgaaattt cttcgattt ttctaggcg atcaaattt 
      ctgattacc tcatcagcc atccggatt tcctggagc 
      gctacatta tggaagagg ttgaagtgg agcaactgg 
      aaaggtgga tcatacgta aatctcaac gtttaaaaa 
      agaaaccgg aaagtgcga gcaggaacg gccggaaaa 
      ccagtcttt cgagatgga agttttgtg tacacgccg 
      acaaacgcc agatgctgc aggatctca ctcacagcc 
      tacaactaa ggcctacga aagatcacg tcctgtggg 
      aattggcca actgcattt gttctgccg ttaatgcag 
      acgggttta ggttgtatc tggtggata aaggtgatc 
      ccgaaagtt tagccctct ttttaggaa ttgagtgca 
      agaatgaaa atggagagc ccaagttac agaaaataa 
      aaagtggag tcagcgttc cattgtatg ttgccaaaa 
      ctcttgaac ctgtgagac tttccagag gcgaggcag 
      atcctcgtt ttccggatg gaataccaa acgatgggg 
      acatcgccc atgttttat gtcaagcgc taatgcagc 
      ccgacgggc aattagccg cagtcctcg aaatgtggc 
      gtcgcgagc acgagggcc ccttctacg aagaggacg 
      aggaaatcg catccagtt atctagtct ggggtggga 
      tagctctgc actacgccc acctttatc tcaacatga 
      acaagcaga agaagctca acgattttc tcctggctg 
      tggtcaaat cgattttcc aagatatgg tcagcatgg 
      acatgctgg tgatgaaaa taatgaact ttgctcaaa 
      gactagtgg atgcggatc gcgcgtccc tatttctgg 
      ttgacaata agagcaaag aattatatg ccactatat 
      ttgatgtgg gagttgaga aagatcaag ttgaatgtt 
      cgaatggca gcggtgaca acgaggagt ttgggaaag 
      tcgacacgt caaaggaga ttcggtttc ctctgggga 
      cgggaatcg caggccagg tggctatga ccggggaag 
      ttctcaaca tcaaagacg cctatgctg attcgaggt 
      ttaaccgca cggtcgatc aacttacag gttatcgaa 
      ccaacacca tcctctgca tgcccattt tcattcgag 
      gctcagtga tcggcgtgg tccagatgg tgaacaaac 
      attccggcc tatttacca tcgaggacg aggaagcgt 
      tcgaaatgt ttgccgttt actgtggac tggccttac 
      atcacgcca aactttacg acaaaatcc gcaaatccg 
      agcagaaat atagagtgg ctctcgaag tattaagtt 
      accacaata gctgtaccg aggaggaat atcaaattg 
      cagtgcgcg agggaattc cggagaaaa ttgtaggaa 
      tcgacgatt actacttca atcccttcc aaatcgaag 
      atttccata aagccatct acgcgatgt acatgttca 
      tcgacctct tcggcctcg ccagattcg acaagaatt 
      gcctcatga ggttctcgc tcactgtga aaaagaatt 
      acagaaggg tgccctatc ataattgga cgcacggct 
      tctccgtcg ccaactcca tgtacagcc tcataaagc 
      attcggcgg gcgcttttc gcccaaacg aatgcctgg 
      cattgtaca taggggcac tgtgtcatg atctcgacc 
      accgcggga agaacaaca aatttatgt tggacacgg 
      aatcgcctc tggctgcca tctattcca catccacca 
      tggaacacc accatttca atcaaacgg tgaccattt 
      tgcaacagg agggccata atatctttg ccaaactct 
      cgagttctg agtacaaac aagtcctaa gtttgctta 
      agcactgca tcctcgcca ccgacttag cagtgttct 
      tccccaaca aagctaaac taagccaat tggtccaaa 
      gcaacgcgt tctcctgga acaacttag agcaccggt 
      tattactgc aagccattt cgatgaccg gcagtgatc 
      tcagtgcga gcgccaagc cgtgggaca tccaagtcg 
      agaccgtta aagtcatct tcgaggagt tttacgagc 
      aaggagatg ccgaaagac aagctggca gaaaaccca 
      ttccgatga tggacaggc accagccgg accaacaag 
      ccgcctctc aagtgggct ttttgacgg gaatttgtg 
      tttcttgct atagtttgc tttatactt tgattcctg 
      aaactaaac cgttattgg acatgtgtc aggaaaatt 
      tggagcgtt ggcggaaaa tcgacaatg aaatcaaag 
      aaaaacaaa tacaaaact gatcaaatc aaacaacac 
      gttcctcct ttgtcgttt attgtcaac aaatgtttt 
      aatgtttaa gtaagagat gtggtagtt tagaacatt 
      caacctttc gtagtgcct ttaggtttt atgattaaa 
      ttacgaaat aattttatt tttgtacat ttttttcga 
      atcgtaata aatgtaagt ggattac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY