Status
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Gene information
General information
TC031065
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC002532,TC002533
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 25460193..25495225
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031065
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03394

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031065 RA
      MTSEKEIID WGEDFDESN YKSCFARSV AVNSQYKQL 
      KTSEPRQLQ QLEEKDSSQ ENFKRLSAR PLPLTQPIV 
      EEIRVRKNS SDDARRLSY SSVSNSRRT SICSSRISE 
      IPVENIVQQ SKVSTLQWQ LKEVEKSRE MYKAVMKQV 
      VSFLEKAHR NLELLGHRL NSKQTVPRS KSEHQIVAG 
      QSDTLNSND DYSMFRDFT WRRPKKPET SSEEIPPEK 
      LSQEAFRLL RTAQSLLNT QEPKLSNDT PDDLEFLAQ 
      LAKEFPQTP DPKPQRTTS FSLSPKLLV PERETRIAT 
      AFNRKLSLQ LNETRRNSF KYSPRNSVI ETPLLSSTL 
      NDFKEHIIL NNDKLKSEK SNSPPAGSI SSVEDESGF 
      SSMNSFQDV GLPVVGDEV TAKNVLLRS MLHNNVEVS 
      DKNGNIEDD VKLWQKPDV AHKRWSSTP VNCSGTKQS 
      LNVLWV
    CDS sequence
    • >TC031065 RA
      atgacaagt gaaaaagaa attatcgat tggggtgag 
      gattttgac gagagcaat tacaaaagt tgcttcgct 
      cgttcagtg gcagtgaat tcacaatat aaacaattg 
      aaaacatcg gaaccacga cagctgcag cagctggag 
      gaaaaagat tcttcccag gagaacttt aagagatta 
      tctgctcga ccgttacca ttaactcaa cctatagtt 
      gaggaaatt agagtgaga aagaattcg tcagatgat 
      gctcgacgg ctgtcctac agtagcgtt agcaatagt 
      agaaggact tccatttgc tcaagccgg atttccgaa 
      attcctgtt gaaaatatt gtccagcag tcgaaagtg 
      agcactttg cagtggcaa ctaaaggag gtggagaag 
      agtcgagaa atgtacaaa gcagtgatg aaacaggtt 
      gtgtccttt ctggaaaag gcacaccgg aacttggaa 
      cttttagga catcgatta aactcgaaa caaacggtc 
      cctaggtcg aaaagtgaa caccaaatt gttgcaggg 
      caaagtgat acactcaac tccaacgac gattattca 
      atgttccgc gatttcacg tggaggcgc cccaagaag 
      cccgaaacc tcctccgag gagatccct cccgagaaa 
      ctgtcccag gaagcattc aggctcctc cgaaccgct 
      caatctcta ttgaacact caagagccg aagctttcc 
      aacgacact ccagacgac ttagaattt ctagcacag 
      ttggcaaaa gagttccct caaacacct gacccgaaa 
      ccgcaacga acaacatcg ttctcccta agccccaaa 
      ctcctagtt cctgaaaga gaaactcgc attgccacc 
      gctttcaac cgcaaactc tccctacaa ctcaacgaa 
      acaagaaga aactccttc aaatattcc cccagaaat 
      agcgtaata gaaacaccg cttttatcc tcaacactc 
      aacgacttc aaggagcac attattctg aataacgat 
      aaactgaag tcggaaaaa tccaactcg ccacctgct 
      ggaagtatc agttctgtg gaggatgag agtggtttt 
      tcttcgatg aattcgttt caggatgtg ggattgccg 
      gttgttggg gacgaagtt acggcaaaa aatgtcttg 
      ttgaggagt atgttacat aataatgtt gaggtttct 
      gataaaaat gggaatatt gaagatgat gtaaaatta 
      tggcagaaa cctgatgta gcccataaa agatggagt 
      tcaactcct gttaactgt tccgggacc aaacaatca 
      ctgaatgta ttatgggtc tag
    mRNA sequence
    • >TC031065 RA
      cgagttgtg gtggcgttc tttcggtgt ttcagtgag 
      gtctcactg tgatcgtag tgaattgtt taacaaatg 
      ttgtccatt aaaacagtg tgacatgtt ttttctaac 
      tgttgtaca aattggaaa tttcactct tactacaag 
      aattaatta aagaaatag cattttttg ttaaaatga 
      caagtgaaa aagaaatta tcgattggg gtgaggatt 
      ttgacgaga gcaattaca aaagttgct tcgctcgtt 
      cagtggcag tgaattcac aatataaac aattgaaaa 
      catcggaac cacgacagc tgcagcagc tggaggaaa 
      aagattctt cccaggaga actttaaga gattatctg 
      ctcgaccgt taccattaa ctcaaccta tagttgagg 
      aaattagag tgagaaaga attcgtcag atgatgctc 
      gacggctgt cctacagta gcgttagca atagtagaa 
      ggacttcca tttgctcaa gccggattt ccgaaattc 
      ctgttgaaa atattgtcc agcagtcga aagtgagca 
      ctttgcagt ggcaactaa aggaggtgg agaagagtc 
      gagaaatgt acaaagcag tgatgaaac aggttgtgt 
      cctttctgg aaaaggcac accggaact tggaacttt 
      taggacatc gattaaact cgaaacaaa cggtcccta 
      ggtcgaaaa gtgaacacc aaattgttg cagggcaaa 
      gtgatacac tcaactcca acgacgatt attcaatgt 
      tccgcgatt tcacgtgga ggcgcccca agaagcccg 
      aaacctcct ccgaggaga tccctcccg agaaactgt 
      cccaggaag cattcaggc tcctccgaa ccgctcaat 
      ctctattga acactcaag agccgaagc tttccaacg 
      acactccag acgacttag aatttctag cacagttgg 
      caaaagagt tccctcaaa cacctgacc cgaaaccgc 
      aacgaacaa catcgttct ccctaagcc ccaaactcc 
      tagttcctg aaagagaaa ctcgcattg ccaccgctt 
      tcaaccgca aactctccc tacaactca acgaaacaa 
      gaagaaact ccttcaaat attccccca gaaatagcg 
      taatagaaa caccgcttt tatcctcaa cactcaacg 
      acttcaagg agcacatta ttctgaata acgataaac 
      tgaagtcgg aaaaatcca actcgccac ctgctggaa 
      gtatcagtt ctgtggagg atgagagtg gtttttctt 
      cgatgaatt cgtttcagg atgtgggat tgccggttg 
      ttggggacg aagttacgg caaaaaatg tcttgttga 
      ggagtatgt tacataata atgttgagg tttctgata 
      aaaatggga atattgaag atgatgtaa aattatggc 
      agaaacctg atgtagccc ataaaagat ggagttcaa 
      ctcctgtta actgttccg ggaccaaac aatcactga 
      atgtattat gggtctagt gctttaatt tcaaatatt 
      tgccattac tactaatta tttatttgt gtatgctgt 
      taataaaaa tgtgataaa ttactaaat tttgtgtct 
      gccaatgga caataataa taataatgt cactttttg 
      caatataat gtgctttga ggtttcaat atttaggac 
      tatttttaa ttcctctaa ttattgcat aaggtagat 
      gaattaaac tattgttat aaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY