Status
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Gene information
General information
TC002507 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 26303723..26332125
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002507
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03390

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 10 (9 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002507 RA
      MSDSDDENF NRDEDCVDL TGFLFGNIN EQGQLESDV 
      LDTESQKQL SNLGKLGLG STLKEMIGV DSIPKDDSD 
      SDYDNSEGQ KSKFGDENK ESNSAESQN NEDSNFESK 
      SPSAVDFSD INELAEEEE EEAKPENYD ADDEHPNEI 
      DNKLMPPPP VPTTKENSE SIVTTDDGD KKKLETPLA 
      AMLPSKYAN VNVTDLFPD FRHGKVLRF SRLFGPGKP 
      SSLPNIWKN VRKKRKRRK HKDSANQHD SDSNSEDEK 
      PKHRGWFID YAEPPPPEQ IQSDDEEKF LKPMENATV 
      DSKAETQSK DDTGPKVAD WRFGPAQIW YDMLEVPET 
      GEGFNYGFK LKEDQPPEP TPEATEPRE GDEFPDDAF 
      LMVTQLHWE DDVVWDGND IKHKVLQKL NSKTNAAGW 
      VPSSGNRTA QAFSQPGKG GLLSGSSVR LPIPAPPLP 
      GMKAKAQIL NKPRPDPEQ DDTWYSIFP VENEDLVYG 
      RWEDDVIWD ADNMKNIPK PSILTLDPN DENIILGIP 
      DDIDPSKQI AGQATPVKV KIPHPHVKK SKILLGKAG 
      VINVLQEDT PPPPPKSPN RDPFNISND VYYMPRSSE 
      TTMRLKVGG GNLIQHSTP VVELRAPFI QTHMGLMRL 
      RNFHRPPMK RFSHGPLAH PGPHPVMPL VKHIKKKAK 
      QREAERMAS GGGDVFFMR TPEDLTGRD GDIILIEFC 
      EEHPPLMNQ VGMCSKIKN YYKRKAAKD SGPPSYKYG 
      ETAYAHTSP FLGILHPGQ SIQAIENNM YRAPIYEHD 
      KPCSDFLVI RTRQQYYIR EIDALYVAG QQCPLYEVP 
      GPNSKRANN FVRDFLQVF IYRLFWKSR DNPRRIKME 
      DIKKAFPSH SESSIRKRL KLCADFKRT GMDSNWWVI 
      KPEFRLPTE EEIRAMVSP EQCCAYFSM VAAEQRLKD 
      AGYGEKFIF TPAEDDDEE MQLKMDDEV KVAPWNTTR 
      AYIQAMKGK CLLQLTGPA DPTGCGEGF SYVRVPNKP 
      TQNKEEQES QPKRTVTGT DADLRRLSL NNAKALLRK 
      FGVPEEEIK KLSRWEVID VVRTLSTEK AKAGEEGMD 
      KFSRGNRFS IAEHQERYK EECQRIFDL QNRVLSSAE 
      VLSTDEGES SDEGSDEDI EEMGKNIEN MLANKKTST 
      QLSLEREEQ ERQELRKMI MEGDEKKGK DKKKDDDDN 
      QEQTNYAQQ GRILKIVRT FRDAEGKEF TRVETVRKP 
      AVIDAYVKI RSTKDDAFI RQFATLDEA QKEEMKREK 
      RRIQEQLRR IKRNQEKER LALGLAASS NPAAGGEKL 
      GSNDSPSSQ SFNISPNKS TVISPPKSR RKTKLKPDL 
      KLKCGACGN VGHMRTNKA CPLYQNSGS NAPMNVAMT 
      EEQEEEIEK QLNTDDEDL VNIDGTKVK LSSKLLKHA 
      EEMKRKTLL LKVPKEAMN AKRRRRGMS DLHCDYLKR 
      HNRTANRRR TDPVVVLST ILENILNEM RDMPDVQPF 
      LFPVNPKKV MDYYRIVQR PMDLQTIRE NLRQKKYQS 
      REEFLADVN QIVENSTLY NGAKSSLTV AAQRMLNKC 
      VERLGEKEE RLMRLEKAI NPLLDDNDQ VALTFILDN 
      VINTKLKAM SESWPFLKP VNKKLVKDY YSIIKRPMD 
      LETISKKVA AHKYHSRHE FLVDIEQIL ENCILYNGR 
      DSPFTEKAE QLVKICKAT LDEYDEHLT QLENKISLA 
      QERAMQDDD QAWMGGEEE NYTIAEPDR ISQASSPDH 
      SLFMKSNID DYDYVDVEG GIQTSEDVT ASVSRPRKR 
      KKKDEAAIL EEDLQFSSE EELDEVPLN EFEDNKGGL 
      VISAEMPME GVNADDDSQ QAAEAMVQL GSMGYYQQN 
      EGDGTDLVY KEESMDVDP NYDPSDFLM SGLPMHKQE 
      EIKIDETVL QPQDSAMKI HDDLAVSES EDEGGNNVG 
      EAPVQEEDD GGDLWF
    CDS sequence
    • >TC002507 RA
      atgagtgat agcgacgac gagaacttt aacagagac 
      gaggactgt gtggacctc accggtttc ctcttcggg 
      aacatcaac gagcaaggg caactcgaa agcgatgtt 
      ttggacact gaatcccaa aaacaactt tccaacctc 
      gggaagctg ggtttgggc tcaaccctt aaagaaatg 
      attggggtt gatagcatc cctaaagac gattccgac 
      agcgattat gataattcc gaggggcaa aaatcgaaa 
      tttggggat gaaaacaaa gaaagcaac agtgcggag 
      tcgcaaaat aatgaagat tctaacttc gagtcaaaa 
      tcgcccagt gctgttgat ttttccgat ataaatgaa 
      ctcgctgag gaagaggag gaggaggcg aaaccggaa 
      aattacgat gctgatgat gagcacccc aatgaaatt 
      gataacaaa ttaatgccg ccacctcca gttccgaca 
      acgaaagaa aattccgaa tcaattgtt acgactgat 
      gatggtgat aaaaagaag ctcgaaacg cctcttgcg 
      gcaatgctg ccgtccaaa tacgcaaac gttaacgtt 
      acggacctt tttccggat ttccggcac ggaaaagtg 
      ttacgattt tcgcggctt tttgggcca ggaaaaccg 
      agcagtttg cccaatatt tggaaaaat gtgaggaag 
      aaacgcaag agaaggaag cacaaagat agcgcgaat 
      cagcacgat tctgattcg aactctgag gatgagaag 
      ccgaaacat cggggctgg tttattgat tatgctgag 
      cctccgcct cctgagcaa attcaaagc gatgatgaa 
      gagaagttt ctgaaacca atggaaaat gcgaccgtt 
      gatagcaaa gctgagacc caatcaaag gatgacacg 
      gggcctaaa gtggccgat tggcgcttt ggccccgcc 
      cagatttgg tacgatatg ttggaggtc ccggagaca 
      ggggagggg tttaattat ggttttaaa ctaaaagaa 
      gatcagccc ccagagccc acacctgaa gccaccgaa 
      ccgcgcgaa ggtgacgaa ttccccgac gatgctttc 
      ctcatggtc acccaactg cactgggag gacgacgtc 
      gtatgggac ggcaacgac attaagcat aaagttttg 
      cagaagttg aattcgaag acgaatgct gcgggttgg 
      gtgccctct agcggaaat cggacagct caggcattt 
      agtcaaccc ggaaaaggc gggttgttg tccggaagt 
      tcggtccgg ttaccgatt ccagcaccg ccactgccc 
      ggaatgaaa gccaaggcg caaatatta aataagccg 
      cgtccggat ccggaacag gatgacacg tggtattcg 
      atttttccg gtcgaaaat gaggatttg gtgtatggg 
      agatgggag gatgatgtt atttgggac gctgataac 
      atgaaaaat attccgaaa ccgtcgatt ctcacactg 
      gatcctaac gatgaaaat attatttta ggcattccg 
      gatgatata gatccgtca aaacaaatt gcaggtcaa 
      gcaacccct gtaaaggta aaaatccca cacccccac 
      gtaaaaaaa tcaaaaatc ctcctcggc aaagccggc 
      gttatcaac gtcctccag gaagacacc ccacctcca 
      cccccaaaa tcaccaaac cgggaccca ttcaacatc 
      tcaaacgac gtttactac atgccgcga tcatccgaa 
      accaccatg cgtctcaaa gtcgggggc ggcaaccta 
      atccaacac tccaccccc gtggtggag ctccgcgcc 
      ccatttatc caaacccat atgggtctg atgcgtctc 
      cgcaacttc catcggcca cccatgaaa cgcttctcg 
      catgggcct ttggcccat ccagggccg catccggtc 
      atgccgttg gtcaagcat atcaaaaag aaggcaaaa 
      caacgagag gcggagcgt atggcttca ggaggcggc 
      gatgtgttc tttatgcgc actccagag gatttgact 
      ggaagggat ggcgatatt attttgatt gagttttgt 
      gaggagcat ccgcctctg atgaaccaa gttgggatg 
      tgttcaaaa atcaagaat tattataag aggaaagcg 
      gcgaaagat tcagggcct cctagttat aagtatggg 
      gagacggcc tatgctcac acttcgccg tttttgggg 
      attttgcat ccaggacag tctattcaa gccattgag 
      aataacatg tacagggcc ccaatttac gagcatgat 
      aagccatgc agcgacttc ctagtgatc agaacgaga 
      caacaatat tatatccgt gaaatagac gctctctac 
      gttgcaggc caacagtgt cctttatac gaagttcca 
      ggaccgaac tcaaaacga gcgaataat tttgtccgc 
      gattttcta caagttttt atttatcga ttgttttgg 
      aaaagtcgc gataatccc cgaagaatt aaaatggaa 
      gatattaag aaagctttt ccttcacat tccgagagt 
      agcataaga aaaagactg aaattatgt gctgatttt 
      aaaagaaca ggaatggac tcaaattgg tgggtgatt 
      aaaccggag tttcggtta ccgacagag gaggaaatc 
      cgggcgatg gtctccccg gaacagtgc tgtgcctat 
      ttcagcatg gtggcggcc gagcaaagg ctcaaggat 
      gctggatac ggcgagaaa tttattttt acgcctgcg 
      gaggatgac gacgaagaa atgcaacta aaaatggac 
      gatgaagta aaagtggct ccgtggaac acgactcga 
      gcgtacatt caagcaatg aaagggaaa tgtttgtta 
      caattaaca ggacctgct gatcctaca ggatgtggg 
      gaaggtttt agttacgtc agagtcccc aataaaccc 
      acacaaaat aaagaagaa caggagtcc cagcctaaa 
      cgtaccgtt accggaacc gacgctgat ttgcgtcgc 
      ctgtctttg aacaacgcc aaagctttg ttgcgcaaa 
      ttcggagtc ccggaggaa gaaatcaaa aaattgtct 
      cgatgggag gtcatagac gtcgtccga acactttcg 
      accgaaaaa gccaaagct ggtgaagag ggaatggat 
      aaattttcg cgtggtaac cgattctca atcgccgaa 
      catcaagaa cgttacaaa gaggagtgt caacgaatt 
      ttcgatttg caaaatcgt gttctatca agcgctgag 
      gttctaagt accgacgaa ggtgaaagt tcggacgaa 
      ggcagcgac gaagatatc gaagaaatg ggcaaaaac 
      attgaaaac atgctcgca aataaaaaa acaagtact 
      cagttgtcg ctcgaacgg gaggaacaa gagcgacaa 
      gagttgcgt aaaatgata atggaaggt gacgagaag 
      aagggcaag gataagaaa aaggacgat gacgataat 
      caggaacag acgaattat gcacaacag ggccggatt 
      ttgaaaatt gttaggacg tttagggac gcggaagga 
      aaggagttt acgcgagtt gaaactgtg cgaaaaccg 
      gcagtgatt gatgcgtat gtgaagatt cggtcgacg 
      aaagatgat gcgtttatt agacagttt gcgacgttg 
      gatgaagca cagaaggag gaaatgaaa agggagaag 
      agacggatt caggagcag ttgaggcgg attaagagg 
      aatcaggag aaggaaagg ttggccttg ggacttgca 
      gcgtcgtcg aatccggcc gcaggcggc gagaagttg 
      ggctcgaat gacagtcct tcgagccaa tccttcaac 
      atctccccc aacaaatct acggtgatt tcaccgcca 
      aaatcgcgc cgaaaaacc aaattaaaa cccgacttg 
      aagctaaaa tgtggcgct tgtggcaac gtgggccac 
      atgcgtacc aacaaagcg tgtcctttg taccaaaac 
      agcggctcc aacgccccc atgaacgtc gcaatgacc 
      gaagaacaa gaagaagaa atcgaaaaa cagctaaac 
      accgacgac gaagatttg gtcaacatt gacggaacc 
      aaagtgaaa ttatcgagc aaattattg aaacacgcc 
      gaagagatg aagcggaaa acgttactc ctgaaagtc 
      cccaaggag gcaatgaac gcgaaacga cgacgaaga 
      ggaatgtcc gacttgcac tgcgactat ttgaagcga 
      cacaatcga acggcgaat cggcgaagg accgatcct 
      gtggtcgtc ttgtccaca attttggag aatattttg 
      aacgaaatg agagacatg cccgacgtt cagcccttc 
      ctgtttccg gtcaatccg aagaaagtg atggactat 
      taccgcatt gtgcaaagg ccgatggat ttgcagaca 
      attcgcgag aatttgcgc cagaaaaaa taccaaagt 
      cgggaagaa ttcctagcc gatgtcaac caaatcgtc 
      gagaattcg acgctttat aacggggct aaaagtagt 
      ttaactgtg gcggcgcaa cgaatgtta aacaaatgt 
      gtcgagagg ttgggcgag aaggaggag aggctgatg 
      aggctcgag aaggcgatt aatccacta cttgacgac 
      aatgatcaa gtcgccttg acgtttatc ctagacaat 
      gtgattaac acgaaattg aaagccatg tcggaatcg 
      tggccgttt ctcaaaccg gttaataaa aaattggtt 
      aaggattat tatagtatc atcaagcgg ccgatggat 
      ttggaaacg atttcgaaa aaagttgcc gcgcataag 
      tatcacagt aggcatgag tttttggtc gatattgaa 
      caaattttg gaaaattgt attttgtac aatggacga 
      gattcaccc tttactgaa aaggcggaa cagttggtt 
      aagatttgt aaagcgact ttggatgag tacgacgaa 
      catttgaca caattagag aataagatt tctttggca 
      caagaacga gcgatgcaa gacgacgat caagcctgg 
      atgggaggg gaagaagag aattacact attgcagaa 
      cctgaccga attagccag gcaagctct cccgaccac 
      agcttgttc atgaaatca aacatcgac gattacgat 
      tatgtggac gtagaaggc ggaatacaa acaagtgaa 
      gacgttaca gcttcagtg tcaaggccg cgaaaacgc 
      aaaaagaag gacgaggct gccatcctt gaagaagat 
      ttgcaattt tcgagcgag gaagagctg gacgaggtc 
      cctttgaac gagttcgag gataacaag ggcggtttg 
      gtgatttcg gccgaaatg cccatggag ggtgtgaac 
      gccgacgat gacagtcag caggcggcc gaagcgatg 
      gtacagttg ggttcgatg ggttactac cagcaaaat 
      gagggcgat ggtactgat ttggtgtac aaggaggag 
      agtatggac gtggatccg aactatgat ccgtctgat 
      tttctcatg tcgggactg ccgatgcat aaacaggag 
      gaaatcaaa atcgatgag acggtgttg cagccgcag 
      gacagtgcg atgaagatt catgatgat ctcgctgtg 
      agtgagagt gaggacgaa ggggggaat aatgtgggg 
      gaggcgccg gtgcaagag gaggatgat ggaggggat 
      ctgtggttc taa
    mRNA sequence
    • >TC002507 RA
      agggcactc acttcgcca tttgtgatt atttatatg 
      gtggaccaa cttttatct attttgtgt tttaattgt 
      ttaaaataa atgtaaaat gagtgatag cgacgacga 
      gaactttaa cagagacga ggactgtgt ggacctcac 
      cggtttcct cttcgggaa catcaacga gcaagggca 
      actcgaaag cgatgtttt ggacactga atcccaaaa 
      acaactttc caacctcgg gaagctggg tttgggctc 
      aacccttaa agaaatgat tggggttga tagcatccc 
      taaagacga ttccgacag cgattatga taattccga 
      ggggcaaaa atcgaaatt tggggatga aaacaaaga 
      aagcaacag tgcggagtc gcaaaataa tgaagattc 
      taacttcga gtcaaaatc gcccagtgc tgttgattt 
      ttccgatat aaatgaact cgctgagga agaggagga 
      ggaggcgaa accggaaaa ttacgatgc tgatgatga 
      gcaccccaa tgaaattga taacaaatt aatgccgcc 
      acctccagt tccgacaac gaaagaaaa ttccgaatc 
      aattgttac gactgatga tggtgataa aaagaagct 
      cgaaacgcc tcttgcggc aatgctgcc gtccaaata 
      cgcaaacgt taacgttac ggacctttt tccggattt 
      ccggcacgg aaaagtgtt acgattttc gcggctttt 
      tgggccagg aaaaccgag cagtttgcc caatatttg 
      gaaaaatgt gaggaagaa acgcaagag aaggaagca 
      caaagatag cgcgaatca gcacgattc tgattcgaa 
      ctctgagga tgagaagcc gaaacatcg gggctggtt 
      tattgatta tgctgagcc tccgcctcc tgagcaaat 
      tcaaagcga tgatgaaga gaagtttct gaaaccaat 
      ggaaaatgc gaccgttga tagcaaagc tgagaccca 
      atcaaagga tgacacggg gcctaaagt ggccgattg 
      gcgctttgg ccccgccca gatttggta cgatatgtt 
      ggaggtccc ggagacagg ggaggggtt taattatgg 
      ttttaaact aaaagaaga tcagccccc agagcccac 
      acctgaagc caccgaacc gcgcgaagg tgacgaatt 
      ccccgacga tgctttcct catggtcac ccaactgca 
      ctgggagga cgacgtcgt atgggacgg caacgacat 
      taagcataa agttttgca gaagttgaa ttcgaagac 
      gaatgctgc gggttgggt gccctctag cggaaatcg 
      gacagctca ggcatttag tcaacccgg aaaaggcgg 
      gttgttgtc cggaagttc ggtccggtt accgattcc 
      agcaccgcc actgcccgg aatgaaagc caaggcgca 
      aatattaaa taagccgcg tccggatcc ggaacagga 
      tgacacgtg gtattcgat ttttccggt cgaaaatga 
      ggatttggt gtatgggag atgggagga tgatgttat 
      ttgggacgc tgataacat gaaaaatat tccgaaacc 
      gtcgattct cacactgga tcctaacga tgaaaatat 
      tattttagg cattccgga tgatataga tccgtcaaa 
      acaaattgc aggtcaagc aacccctgt aaaggtaaa 
      aatcccaca cccccacgt aaaaaaatc aaaaatcct 
      cctcggcaa agccggcgt tatcaacgt cctccagga 
      agacacccc acctccacc cccaaaatc accaaaccg 
      ggacccatt caacatctc aaacgacgt ttactacat 
      gccgcgatc atccgaaac caccatgcg tctcaaagt 
      cgggggcgg caacctaat ccaacactc cacccccgt 
      ggtggagct ccgcgcccc atttatcca aacccatat 
      gggtctgat gcgtctccg caacttcca tcggccacc 
      catgaaacg cttctcgca tgggccttt ggcccatcc 
      agggccgca tccggtcat gccgttggt caagcatat 
      caaaaagaa ggcaaaaca acgagaggc ggagcgtat 
      ggcttcagg aggcggcga tgtgttctt tatgcgcac 
      tccagagga tttgactgg aagggatgg cgatattat 
      tttgattga gttttgtga ggagcatcc gcctctgat 
      gaaccaagt tgggatgtg ttcaaaaat caagaatta 
      ttataagag gaaagcggc gaaagattc agggcctcc 
      tagttataa gtatgggga gacggccta tgctcacac 
      ttcgccgtt tttggggat tttgcatcc aggacagtc 
      tattcaagc cattgagaa taacatgta cagggcccc 
      aatttacga gcatgataa gccatgcag cgacttcct 
      agtgatcag aacgagaca acaatatta tatccgtga 
      aatagacgc tctctacgt tgcaggcca acagtgtcc 
      tttatacga agttccagg accgaactc aaaacgagc 
      gaataattt tgtccgcga ttttctaca agtttttat 
      ttatcgatt gttttggaa aagtcgcga taatccccg 
      aagaattaa aatggaaga tattaagaa agcttttcc 
      ttcacattc cgagagtag cataagaaa aagactgaa 
      attatgtgc tgattttaa aagaacagg aatggactc 
      aaattggtg ggtgattaa accggagtt tcggttacc 
      gacagagga ggaaatccg ggcgatggt ctccccgga 
      acagtgctg tgcctattt cagcatggt ggcggccga 
      gcaaaggct caaggatgc tggatacgg cgagaaatt 
      tatttttac gcctgcgga ggatgacga cgaagaaat 
      gcaactaaa aatggacga tgaagtaaa agtggctcc 
      gtggaacac gactcgagc gtacattca agcaatgaa 
      agggaaatg tttgttaca attaacagg acctgctga 
      tcctacagg atgtgggga aggttttag ttacgtcag 
      agtccccaa taaacccac acaaaataa agaagaaca 
      ggagtccca gcctaaacg taccgttac cggaaccga 
      cgctgattt gcgtcgcct gtctttgaa caacgccaa 
      agctttgtt gcgcaaatt cggagtccc ggaggaaga 
      aatcaaaaa attgtctcg atgggaggt catagacgt 
      cgtccgaac actttcgac cgaaaaagc caaagctgg 
      tgaagaggg aatggataa attttcgcg tggtaaccg 
      attctcaat cgccgaaca tcaagaacg ttacaaaga 
      ggagtgtca acgaatttt cgatttgca aaatcgtgt 
      tctatcaag cgctgaggt tctaagtac cgacgaagg 
      tgaaagttc ggacgaagg cagcgacga agatatcga 
      agaaatggg caaaaacat tgaaaacat gctcgcaaa 
      taaaaaaac aagtactca gttgtcgct cgaacggga 
      ggaacaaga gcgacaaga gttgcgtaa aatgataat 
      ggaaggtga cgagaagaa gggcaagga taagaaaaa 
      ggacgatga cgataatca ggaacagac gaattatgc 
      acaacaggg ccggatttt gaaaattgt taggacgtt 
      tagggacgc ggaaggaaa ggagtttac gcgagttga 
      aactgtgcg aaaaccggc agtgattga tgcgtatgt 
      gaagattcg gtcgacgaa agatgatgc gtttattag 
      acagtttgc gacgttgga tgaagcaca gaaggagga 
      aatgaaaag ggagaagag acggattca ggagcagtt 
      gaggcggat taagaggaa tcaggagaa ggaaaggtt 
      ggccttggg acttgcagc gtcgtcgaa tccggccgc 
      aggcggcga gaagttggg ctcgaatga cagtccttc 
      gagccaatc cttcaacat ctcccccaa caaatctac 
      ggtgatttc accgccaaa atcgcgccg aaaaaccaa 
      attaaaacc cgacttgaa gctaaaatg tggcgcttg 
      tggcaacgt gggccacat gcgtaccaa caaagcgtg 
      tcctttgta ccaaaacag cggctccaa cgcccccat 
      gaacgtcgc aatgaccga agaacaaga agaagaaat 
      cgaaaaaca gctaaacac cgacgacga agatttggt 
      caacattga cggaaccaa agtgaaatt atcgagcaa 
      attattgaa acacgccga agagatgaa gcggaaaac 
      gttactcct gaaagtccc caaggaggc aatgaacgc 
      gaaacgacg acgaagagg aatgtccga cttgcactg 
      cgactattt gaagcgaca caatcgaac ggcgaatcg 
      gcgaaggac cgatcctgt ggtcgtctt gtccacaat 
      tttggagaa tattttgaa cgaaatgag agacatgcc 
      cgacgttca gcccttcct gtttccggt caatccgaa 
      gaaagtgat ggactatta ccgcattgt gcaaaggcc 
      gatggattt gcagacaat tcgcgagaa tttgcgcca 
      gaaaaaata ccaaagtcg ggaagaatt cctagccga 
      tgtcaacca aatcgtcga gaattcgac gctttataa 
      cggggctaa aagtagttt aactgtggc ggcgcaacg 
      aatgttaaa caaatgtgt cgagaggtt gggcgagaa 
      ggaggagag gctgatgag gctcgagaa ggcgattaa 
      tccactact tgacgacaa tgatcaagt cgccttgac 
      gtttatcct agacaatgt gattaacac gaaattgaa 
      agccatgtc ggaatcgtg gccgtttct caaaccggt 
      taataaaaa attggttaa ggattatta tagtatcat 
      caagcggcc gatggattt ggaaacgat ttcgaaaaa 
      agttgccgc gcataagta tcacagtag gcatgagtt 
      tttggtcga tattgaaca aattttgga aaattgtat 
      tttgtacaa tggacgaga ttcaccctt tactgaaaa 
      ggcggaaca gttggttaa gatttgtaa agcgacttt 
      ggatgagta cgacgaaca tttgacaca attagagaa 
      taagatttc tttggcaca agaacgagc gatgcaaga 
      cgacgatca agcctggat gggagggga agaagagaa 
      ttacactat tgcagaacc tgaccgaat tagccaggc 
      aagctctcc cgaccacag cttgttcat gaaatcaaa 
      catcgacga ttacgatta tgtggacgt agaaggcgg 
      aatacaaac aagtgaaga cgttacagc ttcagtgtc 
      aaggccgcg aaaacgcaa aaagaagga cgaggctgc 
      catccttga agaagattt gcaattttc gagcgagga 
      agagctgga cgaggtccc tttgaacga gttcgagga 
      taacaaggg cggtttggt gatttcggc cgaaatgcc 
      catggaggg tgtgaacgc cgacgatga cagtcagca 
      ggcggccga agcgatggt acagttggg ttcgatggg 
      ttactacca gcaaaatga gggcgatgg tactgattt 
      ggtgtacaa ggaggagag tatggacgt ggatccgaa 
      ctatgatcc gtctgattt tctcatgtc gggactgcc 
      gatgcataa acaggagga aatcaaaat cgatgagac 
      ggtgttgca gccgcagga cagtgcgat gaagattca 
      tgatgatct cgctgtgag tgagagtga ggacgaagg 
      ggggaataa tgtggggga ggcgccggt gcaagagga 
      ggatgatgg aggggatct gtggttcta aactttgtt 
      tagtgtttt tatattgtt aaatattta ttgaagtaa 
      aatataatg taggcacaa ag
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