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Gene information
General information
TC002499 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid25: 1061912..1075180
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002499
    iBeetle screen iB_03388
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    head mostly not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    head appendages random not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    Comment: abdomen partially upended (inside-outed)
    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    head irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Comment: abdomen partially upended (inside-outed)
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Comment: abdomen partially upended (inside-outed)
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 90.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt not completely molted - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 4 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (22 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    Adult morphology
    motility decreased - Number of animals showing the phenotype: 3 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002499 RA
      MKGWFDAFR YDGGPTLYS FNNRTAVTG DVTLITFLT 
      IFSTLYVAF LIIFPGIRK ERFTTFFAV TLSLFVGAT 
      ILVTLFGSA WHVATASIV STYRAFSTD KLSSNIGVY 
      VGLRHVNIT LQASGGHNW TSDIDFNEQ FLWINSDQM 
      GDSFREAMV RGLPFPILT VAEYFTMGQ EGLSWGGQY 
      RAAGYYAII MLWTSFSVW ILMNLMLIV VPRYGAVLM 
      TTCGFLLLA TACGYFGLL PETPLVVHI EGSTLHFSF 
      GWCYWLVLI AGSICLLSG VIITVLEII FPHSFSTIL 
      EVDYDTPYD RHIIIEDSR GRRFQKKRS SNSKLEEPV 
      GIGTKILRR LSSKTREEK PHDLLPNSH IQNFELGAK 
      SNWSYPHSQ DNIHRSISQ ESITSPRNL PTIHRVIPR 
      PDKMQEVSM W
    CDS sequence
    • >TC002499 RA
      atgaagggc tggttcgat gccttccgc tacgatgga 
      ggtcccact ctttacagt ttcaataac cgaactgcc 
      gtcaccggc gacgtgaca cttatcaca ttcctcacc 
      attttcagt accctttat gtggcattt ttgatcata 
      tttccgggt attagaaaa gagagattt accacattt 
      tttgcggtc actttgagc ctgtttgtc ggagccacc 
      attttagtg actcttttc ggctcagca tggcatgta 
      gcgactgca tctatcgtc agcacttat cgggcgttt 
      tccaccgac aagctctcc tcaaatatt ggggtttac 
      gtaggactg agacatgta aatatcact cttcaagca 
      tctggtggt cataactgg accagtgat atcgacttc 
      aatgagcag tttttgtgg atcaattcg gaccaaatg 
      ggcgatagt tttcgggaa gccatggtt cgagggctc 
      ccatttccc attctgacc gtcgctgag tactttacg 
      atgggtcag gaagggcta tcatggggt ggacagtac 
      cgggcggct gggtattac gccattatc atgttatgg 
      acatcgttt tcagtatgg attttgatg aatctgatg 
      ctaattgtg gtgccgcga tatggagct gttcttatg 
      acaacttgt ggtttttta ctacttgcc acggcttgt 
      ggctacttt ggactgtta ccagaaaca cccttggtt 
      gtgcatatt gagggtagt actctccac tttagcttc 
      gggtggtgc tattggcta gtccttatt gcaggaagc 
      atttgcttg ctgtctggt gtcataata accgtcctg 
      gagatcatc ttcccgcac agtttttcg acgatcctg 
      gaggtggat tatgacact ccttacgac cgtcacatc 
      atcattgaa gacagtcga gggaggagg ttccagaag 
      aagaggtcc agcaatagc aagttggag gaaccggtg 
      ggaatcggc acgaaaatt ctccgtcgg ttatcttcg 
      aaaacgcgt gaagagaaa ccgcacgat ttgctaccc 
      aacagccat atccaaaat ttcgagttg ggggcaaaa 
      tcgaactgg tcgtatcct cacagtcaa gacaatatc 
      cacagaagc atatcgcaa gaatctatc acgtctccg 
      agaaatctc cccacgatt catagagtc atccctagg 
      cccgacaaa atgcaagag gtttccatg tggtga
    mRNA sequence
    • >TC002499 RA
      cagctctta tcagtcgtt cggcgcgct ctcagcaca 
      cattttcac ataaatggt ggttttagt gacgcaaag 
      tgaaactct tcattcggt ggattattt tcactagaa 
      acagtcgtg ttcctctgc catgaaggg ctggttcga 
      tgccttccg ctacgatgg aggtcccac tctttacag 
      tttcaataa ccgaactgc cgtcaccgg cgacgtgac 
      acttatcac attcctcac cattttcag tacccttta 
      tgtggcatt tttgatcat atttccggg tattagaaa 
      agagagatt taccacatt ttttgcggt cactttgag 
      cctgtttgt cggagccac cattttagt gactctttt 
      cggctcagc atggcatgt agcgactgc atctatcgt 
      cagcactta tcgggcgtt ttccaccga caagctctc 
      ctcaaatat tggggttta cgtaggact gagacatgt 
      aaatatcac tcttcaagc atctggtgg tcataactg 
      gaccagtga tatcgactt caatgagca gtttttgtg 
      gatcaattc ggaccaaat gggcgatag ttttcggga 
      agccatggt tcgagggct cccatttcc cattctgac 
      cgtcgctga gtactttac gatgggtca ggaagggct 
      atcatgggg tggacagta ccgggcggc tgggtatta 
      cgccattat catgttatg gacatcgtt ttcagtatg 
      gattttgat gaatctgat gctaattgt ggtgccgcg 
      atatggagc tgttcttat gacaacttg tggtttttt 
      actacttgc cacggcttg tggctactt tggactgtt 
      accagaaac acccttggt tgtgcatat tgagggtag 
      tactctcca ctttagctt cgggtggtg ctattggct 
      agtccttat tgcaggaag catttgctt gctgtctgg 
      tgtcataat aaccgtcct ggagatcat cttcccgca 
      cagtttttc gacgatcct ggaggtgga ttatgacac 
      tccttacga ccgtcacat catcattga agacagtcg 
      agggaggag gttccagaa gaagaggtc cagcaatag 
      caagttgga ggaaccggt gggaatcgg cacgaaaat 
      tctccgtcg gttatcttc gaaaacgcg tgaagagaa 
      accgcacga tttgctacc caacagcca tatccaaaa 
      tttcgagtt gggggcaaa atcgaactg gtcgtatcc 
      tcacagtca agacaatat ccacagaag catatcgca 
      agaatctat cacgtctcc gagaaatct ccccacgat 
      tcatagagt catccctag gcccgacaa aatgcaaga 
      ggtttccat gtggtgacg ttgtgtgac ttgacttga 
      ttttgattt gagtgcaat ttttaaatt ttaagtgat 
      ttttcttgt gatatatgt aaggaagtt gtgtgtaag 
      ttgccatat ttatacgac gtttacaaa ttgaagtga 
      ccgaggaat ttgtaaaga attttatcg agcggatat 
      tatttataa taaacgtat tttgtgcag