Status
Please wait ...
Gene information
General information
TC002497 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 26484601..26488624
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002497
    iBeetle screen iB_03386

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    potentially confused/crosscontaminated with : adults of..., iB # (comma separated) 03396
    Adult morphology
    potentially confused/crosscontaminated with : adults of..., iB # (comma separated) 03396
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    potentially confused/crosscontaminated with : adults of..., iB # (comma separated) 03396
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002497 RA
      MGNHTVKND TSDEDYTSS DEFDPSNHT FINEKWIIR 
      STAQIAIES SIFAPIVLF GIFGNFLSI YVLVKNRHI 
      CTPTNLLIG NMAAADLLS LLIHPWVFL IYDFFQNYQ 
      FGSFGCKTE AAVECSILL ASVISVTAI SYDRLTAIV 
      LPQESKLTK RGAKIVMVA TWVTGILLS TPFYIYRSY 
      KERQWLDFL EKYCTENTM ITNIYWHVI ITVLVWLPL 
      TIQLICYIT IFIKLNKYE KVVRRNLDH RQVDYKKKA 
      VRMMVSVIL TFLICRLPF TALIFYRDI KQSNLSQNT 
      QIQNMASGA YHNLWFASK FFIFVNSAL NPLIYSVTN 
      DKFRRGFRT TKLSKWLFP REKNKPKVA STKPTPKLT 
      DTSMLFKKK NIFLIFKKK KNQPLSIES TVTSTGKAH 
      LEINKI
    CDS sequence
    • >TC002497 RA
      atgggcaac cacaccgtc aaaaacgac acaagcgac 
      gaagactac acctcatcg gacgaattt gacccatcc 
      aaccacact ttcatcaac gaaaaatgg ataattcgc 
      tcaacggct cagatcgca atcgaatct tcgattttc 
      gcaccgatt gtcttgttt ggaattttc ggtaacttt 
      ttatcaatc tacgtgtta gtcaaaaat cggcacatt 
      tgcacccca actaattta ctaatcggg aacatggca 
      gctgctgat ttactctcc cttctgata cacccttgg 
      gtttttttg atctacgat tttttccaa aactatcag 
      ttcggcagc tttggttgt aaaactgaa gccgctgtt 
      gaatgttcg atactgctg gcgagtgtt ataagtgtg 
      acggcaatc agctatgat cgcttgacg gcaattgtg 
      ttgccacaa gagtcgaag ttgacgaaa cgtggggct 
      aaaattgtg atggtcgcc acgtgggtc accggaatt 
      ttgctatcg acccctttt tacatctac cggagttac 
      aaggaacga caatggctc gatttcttg gaaaagtat 
      tgcactgag aacacaatg ataacgaat atttactgg 
      catgtgatt atcacagtg cttgtctgg ctgccactc 
      accatccaa ttgatctgt tatatcacc atttttatc 
      aagttaaat aaatacgaa aaagtggtt cgaaggaat 
      ctggatcac cgtcaagta gattacaag aaaaaagcc 
      gtccgaatg atggtttcg gtaatttta acatttcta 
      atatgcaga ttgcctttt actgcgctc attttttac 
      cgtgacatc aagcagagt aatctatcg caaaatact 
      caaatccaa aatatggca agtggagca taccataat 
      ctttggttc gcgtcaaaa ttcttcatt tttgtcaat 
      tcggcgctc aatccgctg atttatagc gtgacgaat 
      gacaagttc cgaagagga ttcagaacg acaaagttg 
      tcgaaatgg ctgtttcca cgcgagaaa aacaagccg 
      aaagttgcg tcaacgaag ccaacccct aaactcacc 
      gatacgtcc atgttgttc aaaaagaag aacattttt 
      ttgattttc aagaagaag aaaaaccag cctctgtcg 
      attgagagt actgttact tcgacggga aaggcacat 
      ttagaaata aataaaata taa
    mRNA sequence
    • >TC002497 RA
      aaacgcaac gatcagatg ggcaaccac accgtcaaa 
      aacgacaca agcgacgaa gactacacc tcatcggac 
      gaatttgac ccatccaac cacactttc atcaacgaa 
      aaatggata attcgctca acggctcag atcgcaatc 
      gaatcttcg attttcgca ccgattgtc ttgtttgga 
      attttcggt aacttttta tcaatctac gtgttagtc 
      aaaaatcgg cacatttgc accccaact aatttacta 
      atcgggaac atggcagct gctgattta ctctccctt 
      ctgatacac ccttgggtt tttttgatc tacgatttt 
      ttccaaaac tatcagttc ggcagcttt ggttgtaaa 
      actgaagcc gctgttgaa tgttcgata ctgctggcg 
      agtgttata agtgtgacg gcaatcagc tatgatcgc 
      ttgacggca attgtgttg ccacaagag tcgaagttg 
      acgaaacgt ggggctaaa attgtgatg gtcgccacg 
      tgggtcacc ggaattttg ctatcgacc cctttttac 
      atctaccgg agttacaag gaacgacaa tggctcgat 
      ttcttggaa aagtattgc actgagaac acaatgata 
      acgaatatt tactggcat gtgattatc acagtgctt 
      gtctggctg ccactcacc atccaattg atctgttat 
      atcaccatt tttatcaag ttaaataaa tacgaaaaa 
      gtggttcga aggaatctg gatcaccgt caagtagat 
      tacaagaaa aaagccgtc cgaatgatg gtttcggta 
      attttaaca tttctaata tgcagattg ccttttact 
      gcgctcatt ttttaccgt gacatcaag cagagtaat 
      ctatcgcaa aatactcaa atccaaaat atggcaagt 
      ggagcatac cataatctt tggttcgcg tcaaaattc 
      ttcattttt gtcaattcg gcgctcaat ccgctgatt 
      tatagcgtg acgaatgac aagttccga agaggattc 
      agaacgaca aagttgtcg aaatggctg tttccacgc 
      gagaaaaac aagccgaaa gttgcgtca acgaagcca 
      acccctaaa ctcaccgat acgtccatg ttgttcaaa 
      aagaagaac atttttttg attttcaag aagaagaaa 
      aaccagcct ctgtcgatt gagagtact gttacttcg 
      acgggaaag gcacattta gaaataaat aaaatataa 
      atttctgga cttttttgt tagaatttt cgttttttg 
      tattcttgt aacttgcac agttggttg ttatattta 
      tggccccgc gaaaaatat gaatattaa tgaaaacaa 
      gattttggt tccgaaata ataaaaaag taataagtc 
      gttgattta ttattaaga attattccg aagaaacga 
      agtaaatta aa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY