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Gene information
General information
TC002495 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 26684368..26691624
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002495
    iBeetle screen iB_03385

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    iBeetle screen iB_07082

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002495 RA
      MFQKFKTKD LHKQDLNDH KFDVREDEE QSVKLQEII 
      DLLVAAGYF RARIKGLSP FDKVVGGMT WCIESCNVD 
      VDVDLLFQE NSTIGQKIA LTEKIVIVL PKLKCPHSI 
      EPHQIQGLD FIHIFPVIQ WLVKRSLEF RQEKSEFVR 
      KYAVYQFHK VFGNNTFEK KETILKNVN IVNEIYGPK 
      RYYRRKNAP PADLQAQVQ ITLLEYGNA IQKHNVETM 
      ANESVEHED LDTEDLKNL AEHYTSLQL EFKTGGLKY 
      LQEKQKATI EEQLRLISE KCAKLASKK TSLEESLQD 
      IKTQDQEFK TKIEDLNAK MKELKQNDN DKRVEEVEE 
      LVMLNENLK LQESQFREH CKKEMAKLQ NKIKEIKSG 
      ETVVENDAE ANKLCEELT ENVKVIWLQ LAKRNRTVV 
      GLGRQIDDV LNSPELAQY QRRFLELYN QVSAKHKET 
      KQYYTLYNT LEDTKFYMK KELSLLNSV SDSYPEAVL 
      SASGKDEFL KQFQNIVEG VKQSKEKVE RKLLDEKRK 
      RDQLSAALQ VLIEQQRKY VAATRQLSI ECKKHEALL 
      AEYKC
    CDS sequence
    • >TC002495 RA
      atgtttcaa aaattcaaa accaaagac ttgcacaaa 
      caagactta aacgaccac aaattcgac gtccgtgaa 
      gatgaggaa cagtcggtc aagctacaa gaaatcatt 
      gacttgtta gtggctgct ggctatttc cgggccagg 
      attaaaggc ctctcccct ttcgacaaa gtcgtaggg 
      ggaatgact tggtgcatt gaatcctgc aatgtggat 
      gtagatgtc gatcttttg tttcaagaa aattccaca 
      attggacaa aaaattgca ctaactgag aaaattgtc 
      atagttttg cccaaatta aagtgtcca cacagtatc 
      gagccgcat caaatacaa ggcttggat tttatccac 
      attttcccc gtgatacaa tggcttgta aaacggtcg 
      ttggagttc agacaagaa aagtccgag tttgtacga 
      aaatacgcc gtttatcaa tttcacaaa gtctttggt 
      aacaacaca tttgaaaaa aaagaaaca attttaaaa 
      aatgtgaac atcgtgaac gaaatttat ggccccaaa 
      aggtactac cggcgaaaa aatgcaccg ccagctgat 
      ttacaagct caagttcaa ataacactc ctcgaatat 
      ggaaatgcc attcaaaaa cacaatgtt gaaactatg 
      gccaatgaa agtgtagaa cacgaagat ttagacacg 
      gaagatttg aaaaatctc gccgaacac tacacttct 
      ttacagctg gaatttaag accggtggt ttaaaatat 
      ctacaagaa aagcaaaaa gcaactatt gaagaacag 
      ctcaggtta ataagcgaa aagtgcgcc aaactcgct 
      agtaaaaaa acctcactg gaagaatcg ttacaagat 
      attaaaaca caagaccag gaattcaaa accaaaatt 
      gaggacttg aatgcaaag atgaaagag ttgaaacaa 
      aatgacaac gacaagcga gttgaagaa gttgaagag 
      ttggtaatg cttaacgaa aatttgaaa ctgcaagaa 
      agccagttt agggaacac tgtaagaaa gaaatggcc 
      aagcttcag aataaaata aaggaaata aaaagtggt 
      gaaacagtt gtggaaaat gatgcagaa gcgaataaa 
      ttatgcgaa gagttaaca gaaaatgtg aaagtgatt 
      tggttacag ttggccaaa agaaataga acagtggtt 
      ggtttgggc agacaaatt gacgatgtt cttaacagt 
      ccagaattg gcccaatac caacgaaga tttctagaa 
      ttgtacaat caagtttcc gcaaagcat aaagaaacg 
      aaacaatac tacacattg tacaatact ctggaagac 
      acaaaattc tacatgaaa aaagaactg tcgttgctt 
      aattcagtc tctgacagc tatccagag gctgtgttg 
      tcagccagt ggcaaggac gagtttttg aaacagttt 
      caaaatatc gttgaaggg gtaaaacag tcgaaggaa 
      aaagttgag agaaaattg ctagatgag aaaagaaaa 
      cgggatcag ttaagtgca gctttgcaa gtcttgata 
      gagcagcag agaaaatat gttgctgcc acgcgtcaa 
      ttaagtatt gagtgcaaa aaacatgaa gctcttctc 
      gctgaatat aaatgttag
    mRNA sequence
    • >TC002495 RA
      agttgtaat aacatgtac gcgttaatt aaaattatt 
      acacaccac atttccctg aaactggta gtggtggca 
      gttcctaaa ttcccaatt ctgtgatag tgacctgtt 
      tggacgatt ttttaatag tttaatgtg atattttaa 
      ccagataag tggttactc gttttgtaa ttttatacc 
      agcgttatc gtaactgtt ttaaataat ctccagcat 
      gtttcaaaa attcaaaac caaagactt gcacaaaca 
      agacttaaa cgaccacaa attcgacgt ccgtgaaga 
      tgaggaaca gtcggtcaa gctacaaga aatcattga 
      cttgttagt ggctgctgg ctatttccg ggccaggat 
      taaaggcct ctccccttt cgacaaagt cgtaggggg 
      aatgacttg gtgcattga atcctgcaa tgtggatgt 
      agatgtcga tcttttgtt tcaagaaaa ttccacaat 
      tggacaaaa aattgcact aactgagaa aattgtcat 
      agttttgcc caaattaaa gtgtccaca cagtatcga 
      gccgcatca aatacaagg cttggattt tatccacat 
      tttccccgt gatacaatg gcttgtaaa acggtcgtt 
      ggagttcag acaagaaaa gtccgagtt tgtacgaaa 
      atacgccgt ttatcaatt tcacaaagt ctttggtaa 
      caacacatt tgaaaaaaa agaaacaat tttaaaaaa 
      tgtgaacat cgtgaacga aatttatgg ccccaaaag 
      gtactaccg gcgaaaaaa tgcaccgcc agctgattt 
      acaagctca agttcaaat aacactcct cgaatatgg 
      aaatgccat tcaaaaaca caatgttga aactatggc 
      caatgaaag tgtagaaca cgaagattt agacacgga 
      agatttgaa aaatctcgc cgaacacta cacttcttt 
      acagctgga atttaagac cggtggttt aaaatatct 
      acaagaaaa gcaaaaagc aactattga agaacagct 
      caggttaat aagcgaaaa gtgcgccaa actcgctag 
      taaaaaaac ctcactgga agaatcgtt acaagatat 
      taaaacaca agaccagga attcaaaac caaaattga 
      ggacttgaa tgcaaagat gaaagagtt gaaacaaaa 
      tgacaacga caagcgagt tgaagaagt tgaagagtt 
      ggtaatgct taacgaaaa tttgaaact gcaagaaag 
      ccagtttag ggaacactg taagaaaga aatggccaa 
      gcttcagaa taaaataaa ggaaataaa aagtggtga 
      aacagttgt ggaaaatga tgcagaagc gaataaatt 
      atgcgaaga gttaacaga aaatgtgaa agtgatttg 
      gttacagtt ggccaaaag aaatagaac agtggttgg 
      tttgggcag acaaattga cgatgttct taacagtcc 
      agaattggc ccaatacca acgaagatt tctagaatt 
      gtacaatca agtttccgc aaagcataa agaaacgaa 
      acaatacta cacattgta caatactct ggaagacac 
      aaaattcta catgaaaaa agaactgtc gttgcttaa 
      ttcagtctc tgacagcta tccagaggc tgtgttgtc 
      agccagtgg caaggacga gtttttgaa acagtttca 
      aaatatcgt tgaaggggt aaaacagtc gaaggaaaa 
      agttgagag aaaattgct agatgagaa aagaaaacg 
      ggatcagtt aagtgcagc tttgcaagt cttgataga 
      gcagcagag aaaatatgt tgctgccac gcgtcaatt 
      aagtattga gtgcaaaaa acatgaagc tcttctcgc 
      tgaatataa atgttaggt aaaatgttt atcagtatg 
      ttggtaata tttgatacc tgggaaatt gtgtttttt 
      tctttacct attatgtgc tatttgccg aattttatt 
      tgcccacta cagtttatc gtgttgtgt aaagaaatc 
      attacaaat cat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY