Status
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Gene information
General information
TC002495 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 26684368..26691624
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC002495
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
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      Status: {{ goTerm.status }}
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      Quotation: {{ goTerm.quotation }}
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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03385

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    iBeetle screen iB_07082

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002495 RA
      MFQKFKTKD LHKQDLNDH KFDVREDEE QSVKLQEII 
DLLVAAGYF RARIKGLSP FDKVVGGMT WCIESCNVD 
VDVDLLFQE NSTIGQKIA LTEKIVIVL PKLKCPHSI 
EPHQIQGLD FIHIFPVIQ WLVKRSLEF RQEKSEFVR 
KYAVYQFHK VFGNNTFEK KETILKNVN IVNEIYGPK 
RYYRRKNAP PADLQAQVQ ITLLEYGNA IQKHNVETM 
ANESVEHED LDTEDLKNL AEHYTSLQL EFKTGGLKY 
LQEKQKATI EEQLRLISE KCAKLASKK TSLEESLQD 
IKTQDQEFK TKIEDLNAK MKELKQNDN DKRVEEVEE 
LVMLNENLK LQESQFREH CKKEMAKLQ NKIKEIKSG 
ETVVENDAE ANKLCEELT ENVKVIWLQ LAKRNRTVV 
GLGRQIDDV LNSPELAQY QRRFLELYN QVSAKHKET 
KQYYTLYNT LEDTKFYMK KELSLLNSV SDSYPEAVL 
SASGKDEFL KQFQNIVEG VKQSKEKVE RKLLDEKRK 
RDQLSAALQ VLIEQQRKY VAATRQLSI ECKKHEALL 
AEYKC
    CDS sequence
    • >TC002495 RA
      atgtttcaa aaattcaaa accaaagac ttgcacaaa 
caagactta aacgaccac aaattcgac gtccgtgaa 
gatgaggaa cagtcggtc aagctacaa gaaatcatt 
gacttgtta gtggctgct ggctatttc cgggccagg 
attaaaggc ctctcccct ttcgacaaa gtcgtaggg 
ggaatgact tggtgcatt gaatcctgc aatgtggat 
gtagatgtc gatcttttg tttcaagaa aattccaca 
attggacaa aaaattgca ctaactgag aaaattgtc 
atagttttg cccaaatta aagtgtcca cacagtatc 
gagccgcat caaatacaa ggcttggat tttatccac 
attttcccc gtgatacaa tggcttgta aaacggtcg 
ttggagttc agacaagaa aagtccgag tttgtacga 
aaatacgcc gtttatcaa tttcacaaa gtctttggt 
aacaacaca tttgaaaaa aaagaaaca attttaaaa 
aatgtgaac atcgtgaac gaaatttat ggccccaaa 
aggtactac cggcgaaaa aatgcaccg ccagctgat 
ttacaagct caagttcaa ataacactc ctcgaatat 
ggaaatgcc attcaaaaa cacaatgtt gaaactatg 
gccaatgaa agtgtagaa cacgaagat ttagacacg 
gaagatttg aaaaatctc gccgaacac tacacttct 
ttacagctg gaatttaag accggtggt ttaaaatat 
ctacaagaa aagcaaaaa gcaactatt gaagaacag 
ctcaggtta ataagcgaa aagtgcgcc aaactcgct 
agtaaaaaa acctcactg gaagaatcg ttacaagat 
attaaaaca caagaccag gaattcaaa accaaaatt 
gaggacttg aatgcaaag atgaaagag ttgaaacaa 
aatgacaac gacaagcga gttgaagaa gttgaagag 
ttggtaatg cttaacgaa aatttgaaa ctgcaagaa 
agccagttt agggaacac tgtaagaaa gaaatggcc 
aagcttcag aataaaata aaggaaata aaaagtggt 
gaaacagtt gtggaaaat gatgcagaa gcgaataaa 
ttatgcgaa gagttaaca gaaaatgtg aaagtgatt 
tggttacag ttggccaaa agaaataga acagtggtt 
ggtttgggc agacaaatt gacgatgtt cttaacagt 
ccagaattg gcccaatac caacgaaga tttctagaa 
ttgtacaat caagtttcc gcaaagcat aaagaaacg 
aaacaatac tacacattg tacaatact ctggaagac 
acaaaattc tacatgaaa aaagaactg tcgttgctt 
aattcagtc tctgacagc tatccagag gctgtgttg 
tcagccagt ggcaaggac gagtttttg aaacagttt 
caaaatatc gttgaaggg gtaaaacag tcgaaggaa 
aaagttgag agaaaattg ctagatgag aaaagaaaa 
cgggatcag ttaagtgca gctttgcaa gtcttgata 
gagcagcag agaaaatat gttgctgcc acgcgtcaa 
ttaagtatt gagtgcaaa aaacatgaa gctcttctc 
gctgaatat aaatgttag
    mRNA sequence
    • >TC002495 RA
      agttgtaat aacatgtac gcgttaatt aaaattatt 
acacaccac atttccctg aaactggta gtggtggca 
gttcctaaa ttcccaatt ctgtgatag tgacctgtt 
tggacgatt ttttaatag tttaatgtg atattttaa 
ccagataag tggttactc gttttgtaa ttttatacc 
agcgttatc gtaactgtt ttaaataat ctccagcat 
gtttcaaaa attcaaaac caaagactt gcacaaaca 
agacttaaa cgaccacaa attcgacgt ccgtgaaga 
tgaggaaca gtcggtcaa gctacaaga aatcattga 
cttgttagt ggctgctgg ctatttccg ggccaggat 
taaaggcct ctccccttt cgacaaagt cgtaggggg 
aatgacttg gtgcattga atcctgcaa tgtggatgt 
agatgtcga tcttttgtt tcaagaaaa ttccacaat 
tggacaaaa aattgcact aactgagaa aattgtcat 
agttttgcc caaattaaa gtgtccaca cagtatcga 
gccgcatca aatacaagg cttggattt tatccacat 
tttccccgt gatacaatg gcttgtaaa acggtcgtt 
ggagttcag acaagaaaa gtccgagtt tgtacgaaa 
atacgccgt ttatcaatt tcacaaagt ctttggtaa 
caacacatt tgaaaaaaa agaaacaat tttaaaaaa 
tgtgaacat cgtgaacga aatttatgg ccccaaaag 
gtactaccg gcgaaaaaa tgcaccgcc agctgattt 
acaagctca agttcaaat aacactcct cgaatatgg 
aaatgccat tcaaaaaca caatgttga aactatggc 
caatgaaag tgtagaaca cgaagattt agacacgga 
agatttgaa aaatctcgc cgaacacta cacttcttt 
acagctgga atttaagac cggtggttt aaaatatct 
acaagaaaa gcaaaaagc aactattga agaacagct 
caggttaat aagcgaaaa gtgcgccaa actcgctag 
taaaaaaac ctcactgga agaatcgtt acaagatat 
taaaacaca agaccagga attcaaaac caaaattga 
ggacttgaa tgcaaagat gaaagagtt gaaacaaaa 
tgacaacga caagcgagt tgaagaagt tgaagagtt 
ggtaatgct taacgaaaa tttgaaact gcaagaaag 
ccagtttag ggaacactg taagaaaga aatggccaa 
gcttcagaa taaaataaa ggaaataaa aagtggtga 
aacagttgt ggaaaatga tgcagaagc gaataaatt 
atgcgaaga gttaacaga aaatgtgaa agtgatttg 
gttacagtt ggccaaaag aaatagaac agtggttgg 
tttgggcag acaaattga cgatgttct taacagtcc 
agaattggc ccaatacca acgaagatt tctagaatt 
gtacaatca agtttccgc aaagcataa agaaacgaa 
acaatacta cacattgta caatactct ggaagacac 
aaaattcta catgaaaaa agaactgtc gttgcttaa 
ttcagtctc tgacagcta tccagaggc tgtgttgtc 
agccagtgg caaggacga gtttttgaa acagtttca 
aaatatcgt tgaaggggt aaaacagtc gaaggaaaa 
agttgagag aaaattgct agatgagaa aagaaaacg 
ggatcagtt aagtgcagc tttgcaagt cttgataga 
gcagcagag aaaatatgt tgctgccac gcgtcaatt 
aagtattga gtgcaaaaa acatgaagc tcttctcgc 
tgaatataa atgttaggt aaaatgttt atcagtatg 
ttggtaata tttgatacc tgggaaatt gtgtttttt 
tctttacct attatgtgc tatttgccg aattttatt 
tgcccacta cagtttatc gtgttgtgt aaagaaatc 
attacaaat cat

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY