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Gene information
General information
TC002488 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid26: 535350..543086
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002488
    iBeetle screen iB_03383
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 60.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 4 (16 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 4 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002488 RA
      MESEESEQI QEVEEESQD SQELAEIPV HLDDSSVSY 
      ATLSLSNSS SNIVRGKYY KQTYRPAWE QMPDFKGWL 
      RGVVGEPTR AYCTYCQKT LHAHRLSLL KHTCTIRHQ 
      KAAQIHNNR KVGKRSVHR NKTQNMETQ EIHAVVLQE 
      SDLNQTEID NEDGDTIEY TEVTSEVED EENAQKDGF 
      DQEIVEEEE EEESTMELH QVTFEKHNQ NFQGNQSKS 
      PISTHVIDT TRGVPVSGL QVSLYKLID GRWTYINEG 
      VTNLNGKFG GFVDRADFS TGRYKLHYD VDRYFEARK 
      QDSMYPFIE VVFDCRAVT ENYHVPVIL SPYGYSTYR 
      GS
    • >TC002488 RB
      MESEESEQI QEVEEESQD SQELAEIPV HLDDSSVSY 
      ATLSLSNSS SNIVRGKYY KQTYRPAWE QMPDFKGWL 
      RGVVGEPTR AYCTYCQKT LHAHRLSLL KHTCTIRHQ 
      KAAQIHNNR KNKTQNMET QEIHAVVLQ ESDLNQTEI 
      DNEDGDTIE YTEVTSEVE DEENAQKDG FDQEIVEEE 
      EEEESTMEL HQVTFEKHN QNFQGNQSK SPISTHVID 
      TTRGVPVSG LQVSLYKLI DGRWTYINE GVTNLNGKF 
      GGFVDRADF STGRYKLHY DVDRYFEAR KQDSMYPFI 
      EVVFDCRAV TENYHVPVI LSPYGYSTY RGS
    CDS sequence
    • >TC002488 RA
      atggaaagc gaagagagt gagcaaatt caagaagtt 
      gaagaagag tctcaagat tcgcaagaa ttggccgaa 
      attccggtg catttagac gactcgtcg gtttcttac 
      gccacattg agtctatca aacagcagt tcgaatatc 
      gttagagga aaatattac aaacagacg tatcgccca 
      gcatgggag caaatgccc gattttaaa ggttggctt 
      agaggagtc gttggggaa cccacgcgg gcctattgc 
      acctactgt cagaaaacg ctccatgcc catcggttg 
      agtctcctc aaacacacg tgtactatc agacaccaa 
      aaagcggca caaattcat aataatagg aaggtagga 
      aagcgttcc gtacacagg aacaagact cagaacatg 
      gagacgcag gaaatccac gccgttgtc cttcaagaa 
      tcagacttg aaccagaca gaaatcgac aatgaagac 
      ggtgatacg attgaatac accgaagtg acgtcagaa 
      gtcgaagac gaggaaaac gcgcagaaa gatggcttc 
      gaccaggag atagttgag gaggaggaa gaagaggaa 
      tccactatg gagctccat caagtcacc tttgagaag 
      cataaccaa aattttcaa ggcaatcag tcgaaatct 
      ccaatttct acacacgtc attgacact acaagaggt 
      gttccagtg tcgggacta caagttagt ctgtataaa 
      ttgattgat ggacgatgg acttacatt aatgaaggt 
      gtaaccaat ttgaatgga aaatttggg gggtttgtt 
      gaccgcgcg gacttttca acagggagg tacaagttg 
      cattatgac gttgatagg tattttgag gcccggaag 
      caggacagt atgtatccc tttattgag gttgttttc 
      gactgtcgt gccgtcact gaaaattac catgtgccc 
      gtcatttta agtccctac gggtactct acatacagg 
      ggatcgtaa
    • >TC002488 RB
      atggaaagc gaagagagt gagcaaatt caagaagtt 
      gaagaagag tctcaagat tcgcaagaa ttggccgaa 
      attccggtg catttagac gactcgtcg gtttcttac 
      gccacattg agtctatca aacagcagt tcgaatatc 
      gttagagga aaatattac aaacagacg tatcgccca 
      gcatgggag caaatgccc gattttaaa ggttggctt 
      agaggagtc gttggggaa cccacgcgg gcctattgc 
      acctactgt cagaaaacg ctccatgcc catcggttg 
      agtctcctc aaacacacg tgtactatc agacaccaa 
      aaagcggca caaattcat aataatagg aagaacaag 
      actcagaac atggagacg caggaaatc cacgccgtt 
      gtccttcaa gaatcagac ttgaaccag acagaaatc 
      gacaatgaa gacggtgat acgattgaa tacaccgaa 
      gtgacgtca gaagtcgaa gacgaggaa aacgcgcag 
      aaagatggc ttcgaccag gagatagtt gaggaggag 
      gaagaagag gaatccact atggagctc catcaagtc 
      acctttgag aagcataac caaaatttt caaggcaat 
      cagtcgaaa tctccaatt tctacacac gtcattgac 
      actacaaga ggtgttcca gtgtcggga ctacaagtt 
      agtctgtat aaattgatt gatggacga tggacttac 
      attaatgaa ggtgtaacc aatttgaat ggaaaattt 
      ggggggttt gttgaccgc gcggacttt tcaacaggg 
      aggtacaag ttgcattat gacgttgat aggtatttt 
      gaggcccgg aagcaggac agtatgtat ccctttatt 
      gaggttgtt ttcgactgt cgtgccgtc actgaaaat 
      taccatgtg cccgtcatt ttaagtccc tacgggtac 
      tctacatac aggggatcg taa
    mRNA sequence
    • >TC002488 RA
      actcgtcgc catattgca gttagttat atttcggct 
      ttttagtaa cttagaaaa ataaccgtt tgctgttgg 
      cgcgtgaaa tggaaagcg aagagagtg agcaaattc 
      aagaagttg aagaagagt ctcaagatt cgcaagaat 
      tggccgaaa ttccggtgc atttagacg actcgtcgg 
      tttcttacg ccacattga gtctatcaa acagcagtt 
      cgaatatcg ttagaggaa aatattaca aacagacgt 
      atcgcccag catgggagc aaatgcccg attttaaag 
      gttggctta gaggagtcg ttggggaac ccacgcggg 
      cctattgca cctactgtc agaaaacgc tccatgccc 
      atcggttga gtctcctca aacacacgt gtactatca 
      gacaccaaa aagcggcac aaattcata ataatagga 
      aggtaggaa agcgttccg tacacagga acaagactc 
      agaacatgg agacgcagg aaatccacg ccgttgtcc 
      ttcaagaat cagacttga accagacag aaatcgaca 
      atgaagacg gtgatacga ttgaataca ccgaagtga 
      cgtcagaag tcgaagacg aggaaaacg cgcagaaag 
      atggcttcg accaggaga tagttgagg aggaggaag 
      aagaggaat ccactatgg agctccatc aagtcacct 
      ttgagaagc ataaccaaa attttcaag gcaatcagt 
      cgaaatctc caatttcta cacacgtca ttgacacta 
      caagaggtg ttccagtgt cgggactac aagttagtc 
      tgtataaat tgattgatg gacgatgga cttacatta 
      atgaaggtg taaccaatt tgaatggaa aatttgggg 
      ggtttgttg accgcgcgg acttttcaa cagggaggt 
      acaagttgc attatgacg ttgataggt attttgagg 
      cccggaagc aggacagta tgtatccct ttattgagg 
      ttgttttcg actgtcgtg ccgtcactg aaaattacc 
      atgtgcccg tcattttaa gtccctacg ggtactcta 
      catacaggg gatcgtaaa aaaatccct tggtagtgt 
      taagactat aaggtttag aataagtag ttattctag 
      gtttcttgc gtgtggtat gaaataatt gagaagaat 
      tttggatat tccgcaatc gaactgaag tttgcataa 
      ttaataaaa aaataatgc cttagattt ttgttctat 
      cacacgcca gtacagtag tttatttct agtagtaga 
      gtaatatat aatttcatg ttgtaaata ttttttaat 
      taattatat ttaattatt tgctctacc ctaccaaat 
      ttgtcacta cattttttt gtataaaag tagacgttc 
      attgaataa agagagtaa tgattgaaa ttttgtttc 
      tacaagtaa taagtgaac cgggaaata tttttttat 
      aatgtttgt tgttatttt taatattcc cagatgaaa 
      gattgtaat ttttcaggc atgtgtgtt gtgtaattt 
      ttatttaaa cctgtcgta ggtattata aaaaaatag 
      taaatgctg aacatacta caaggcaaa tatttattt 
      tttctaaaa accccaaaa attaatata ctttttatc 
      attat
    • >TC002488 RB
      actcgtcgc catattgca gttagttat atttcggct 
      ttttagtaa cttagaaaa ataaccgtt tgctgttgg 
      cgcgtgaaa tggaaagcg aagagagtg agcaaattc 
      aagaagttg aagaagagt ctcaagatt cgcaagaat 
      tggccgaaa ttccggtgc atttagacg actcgtcgg 
      tttcttacg ccacattga gtctatcaa acagcagtt 
      cgaatatcg ttagaggaa aatattaca aacagacgt 
      atcgcccag catgggagc aaatgcccg attttaaag 
      gttggctta gaggagtcg ttggggaac ccacgcggg 
      cctattgca cctactgtc agaaaacgc tccatgccc 
      atcggttga gtctcctca aacacacgt gtactatca 
      gacaccaaa aagcggcac aaattcata ataatagga 
      agaacaaga ctcagaaca tggagacgc aggaaatcc 
      acgccgttg tccttcaag aatcagact tgaaccaga 
      cagaaatcg acaatgaag acggtgata cgattgaat 
      acaccgaag tgacgtcag aagtcgaag acgaggaaa 
      acgcgcaga aagatggct tcgaccagg agatagttg 
      aggaggagg aagaagagg aatccacta tggagctcc 
      atcaagtca cctttgaga agcataacc aaaattttc 
      aaggcaatc agtcgaaat ctccaattt ctacacacg 
      tcattgaca ctacaagag gtgttccag tgtcgggac 
      tacaagtta gtctgtata aattgattg atggacgat 
      ggacttaca ttaatgaag gtgtaacca atttgaatg 
      gaaaatttg gggggtttg ttgaccgcg cggactttt 
      caacaggga ggtacaagt tgcattatg acgttgata 
      ggtattttg aggcccgga agcaggaca gtatgtatc 
      cctttattg aggttgttt tcgactgtc gtgccgtca 
      ctgaaaatt accatgtgc ccgtcattt taagtccct 
      acgggtact ctacataca ggggatcgt aaaaaaatc 
      ccttggtag tgttaagac tataaggtt tagaataag 
      tagttattc taggtttct tgcgtgtgg tatgaaata 
      attgagaag aattttgga tattccgca atcgaactg 
      aagtttgca taattaata aaaaaataa tgccttaga 
      tttttgttc tatcacacg ccagtacag tagtttatt 
      tctagtagt agagtaata tataatttc atgttgtaa 
      atatttttt aattaatta tatttaatt atttgctct 
      accctacca aatttgtca ctacatttt tttgtataa 
      aagtagacg ttcattgaa taaagagag taatgattg 
      aaattttgt ttctacaag taataagtg aaccgggaa 
      atatttttt tataatgtt tgttgttat ttttaatat 
      tcccagatg aaagattgt aatttttca ggcatgtgt 
      gttgtgtaa tttttattt aaacctgtc gtaggtatt 
      ataaaaaaa tagtaaatg ctgaacata ctacaaggc 
      aaatattta ttttttcta aaaacccca aaaattaat 
      atacttttt atcattat