Status
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Gene information
General information
TC002470 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 27828902..27831693
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002470
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03381

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002470 RA
      MKKITVFLL LINFLIPTT TQSETSEAE LLYDPSENI 
      EILDIHNFH TKIENSQTP WLVKFYLGW CGTCQKLST 
      EWKKFRNEI TPWTDLVKV GAISCSDPA NTPVCLNSN 
      ISAYPTIKY FPENYKSGD DSTVILKGE HLDVKALKK 
      RVIGQIRTE ISEKRGQMY PKLSTYEHP NLEKMFEGL 
      GESVKYVVL VVEAPDQCF ATEIALNLH KNRNVSVKF 
      APNTNTDLV NNLQVSTFP SVVVLDRNN NVSKRFTDP 
      EGVAIESFL LSYGLEVNR IEPNTTKKI VPKKLLRLN 
      QRVKKMGDV VFQVDLEAA LRYSLKQEV SAVRVITNE 
      RLDALRAYL TVVKKYFPF GENNELITQ LVKLTSSSE 
      QVQGVEIFQ LVQKAETNR AFSSVQTYL GCLGSVSGK 
      RRYPCSLWQ LFHYLTVNS DDETNPREV LTAMHGYIR 
      HFFGCGGCS RHFQQMAIE RNLSGVSSL KEAVLWLWE 
      AHNVVNQRL KGDVTEDPE FLKDKFPAK LRCVECYEE 
      DGTWRRNEV FEYLKKMYG KFNVRYVGS DTRVLFPDL 
      DNMF
    CDS sequence
    • >TC002470 RA
      atgaagaaa atcacagtt ttcctcctc ctcatcaat 
      tttctaatc ccaaccacc acacaaagt gaaacaagt 
      gaggctgaa ttattatac gaccccagt gaaaacatc 
      gaaatcctg gacattcac aactttcac accaaaatc 
      gaaaactca caaacaccc tggttagta aaattctac 
      ctgggctgg tgcggcact tgccagaaa ctttcaact 
      gaatggaaa aaattccgc aacgaaatc accccatgg 
      acggatttg gtcaaagtt ggggccatt tcatgctca 
      gaccctgcc aacaccccc gtctgtctc aactccaac 
      atcagtgct taccccaca attaaatat ttccccgaa 
      aattacaaa agtggtgat gattctact gtgatactc 
      aagggcgaa catttggac gttaaagcg ctcaaaaag 
      cgagtaatt gggcaaatt aggaccgaa atcagcgaa 
      aaacgaggc caaatgtac cccaagtta tcgacatac 
      gagcaccca aaccttgaa aaaatgttc gaaggttta 
      ggtgaaagt gtcaagtac gtggttttg gtggtggaa 
      gctccagat cagtgtttt gcgaccgaa atcgcccta 
      aacttgcac aaaaatcga aatgtttcg gttaaattt 
      gccccaaac actaacaca gacttggtt aataatttg 
      caagttagt acgtttcca agtgtcgtg gtcctggac 
      cgtaataac aacgtttcg aaacgattt acggaccca 
      gagggtgtt gctattgag tcgtttctg ctcagttat 
      ggtttagaa gttaataga attgaacca aacactacg 
      aaaaaaatt gttccaaaa aaattatta cgtctgaat 
      caaagggtg aaaaaaatg ggcgatgtt gttttccag 
      gtggatttg gaagctgcg ttaagatac tcactgaag 
      caggaagtt tcagcagtt agagttata acaaacgag 
      cgtttggac gcactaagg gcttactta actgtggtt 
      aaaaaatat ttcccgttt ggtgaaaat aacgaactt 
      ataacccaa ttagttaaa cttacatcg agcagtgaa 
      caagttcaa ggtgttgaa atttttcaa cttgtgcaa 
      aaagctgag acaaatcgg gcgttttcg tccgttcaa 
      acctaccta ggttgtctt ggtagtgta agtggaaaa 
      cgaagatat ccctgtagt ttatggcaa ctttttcac 
      tacttgacc gttaattct gacgatgaa acaaacccg 
      agagaagtc ctaacagca atgcatggc tacatcagg 
      cattttttc ggctgtggt ggttgcagt cgccacttt 
      caacaaatg gcaattgag cggaatctg tcaggtgtt 
      tcctcgctt aaagaagcg gttttgtgg ctgtgggag 
      gctcataat gtcgtgaat caacgtttg aagggtgat 
      gttactgaa gatcccgag tttttgaaa gacaagttt 
      ccggcaaag ttgaggtgt gtggagtgt tatgaagaa 
      gatgggacg tggaggagg aatgaagtg tttgaatat 
      ttgaaaaaa atgtacggg aagtttaat gttagatat 
      gttgggtct gatacaagg gtgttgttt cccgatctg 
      gacaatatg ttttaa
    mRNA sequence
    • >TC002470 RA
      actcaatca tatcgtact cattaccag tcctaaatg 
      ccttccttg tggttaaat aaacatcaa acttcgcca 
      acaccatga agaaaatca cagttttcc tcctcctca 
      tcaattttc taatcccaa ccaccacac aaagtgaaa 
      caagtgagg ctgaattat tatacgacc ccagtgaaa 
      acatcgaaa tcctggaca ttcacaact ttcacacca 
      aaatcgaaa actcacaaa caccctggt tagtaaaat 
      tctacctgg gctggtgcg gcacttgcc agaaacttt 
      caactgaat ggaaaaaat tccgcaacg aaatcaccc 
      catggacgg atttggtca aagttgggg ccatttcat 
      gctcagacc ctgccaaca cccccgtct gtctcaact 
      ccaacatca gtgcttacc ccacaatta aatatttcc 
      ccgaaaatt acaaaagtg gtgatgatt ctactgtga 
      tactcaagg gcgaacatt tggacgtta aagcgctca 
      aaaagcgag taattgggc aaattagga ccgaaatca 
      gcgaaaaac gaggccaaa tgtacccca agttatcga 
      catacgagc acccaaacc ttgaaaaaa tgttcgaag 
      gtttaggtg aaagtgtca agtacgtgg ttttggtgg 
      tggaagctc cagatcagt gttttgcga ccgaaatcg 
      ccctaaact tgcacaaaa atcgaaatg tttcggtta 
      aatttgccc caaacacta acacagact tggttaata 
      atttgcaag ttagtacgt ttccaagtg tcgtggtcc 
      tggaccgta ataacaacg tttcgaaac gatttacgg 
      acccagagg gtgttgcta ttgagtcgt ttctgctca 
      gttatggtt tagaagtta atagaattg aaccaaaca 
      ctacgaaaa aaattgttc caaaaaaat tattacgtc 
      tgaatcaaa gggtgaaaa aaatgggcg atgttgttt 
      tccaggtgg atttggaag ctgcgttaa gatactcac 
      tgaagcagg aagtttcag cagttagag ttataacaa 
      acgagcgtt tggacgcac taagggctt acttaactg 
      tggttaaaa aatatttcc cgtttggtg aaaataacg 
      aacttataa cccaattag ttaaactta catcgagca 
      gtgaacaag ttcaaggtg ttgaaattt ttcaacttg 
      tgcaaaaag ctgagacaa atcgggcgt tttcgtccg 
      ttcaaacct acctaggtt gtcttggta gtgtaagtg 
      gaaaacgaa gatatccct gtagtttat ggcaacttt 
      ttcactact tgaccgtta attctgacg atgaaacaa 
      acccgagag aagtcctaa cagcaatgc atggctaca 
      tcaggcatt ttttcggct gtggtggtt gcagtcgcc 
      actttcaac aaatggcaa ttgagcgga atctgtcag 
      gtgtttcct cgcttaaag aagcggttt tgtggctgt 
      gggaggctc ataatgtcg tgaatcaac gtttgaagg 
      gtgatgtta ctgaagatc ccgagtttt tgaaagaca 
      agtttccgg caaagttga ggtgtgtgg agtgttatg 
      aagaagatg ggacgtgga ggaggaatg aagtgtttg 
      aatatttga aaaaaatgt acgggaagt ttaatgtta 
      gatatgttg ggtctgata caagggtgt tgtttcccg 
      atctggaca atatgtttt aaattataa actaaaaaa 
      aaaaaaatt cccggcgca tccaccaaa attgtaaat 
      ttaaaaaaa tcaatgcca gttaatcat aattgttag 
      atatgtttt aaattatga actaaaaaa tagcttttt 
      ttctctaac aattgttgc cccggcgca tccaccaaa 
      attggaaaa ttcaaaatc aatgccagt tattcataa 
      ttgtttttt tattaagtt ttagttata atgtttata 
      taagacccg taataataa aatatttaa ttcaaaact 
      gctaaattt atctttatt gaagcaaaa ttaagacat 
      aatccaaag aatatattt attggctct aacacaaac 
      aaatgcatc taactaatt tcttagcat atttagaca 
      aaattatgt aaaatagct agtttttta ctgagaaag 
      aatagaaaa tattgcaaa acgtttctt tatagaaaa 
      aaggatttt ttatcaaac gctgaaaag tagaacggt 
      tggattttt ttgtccaat ttaaataaa atctcgcga 
      aatattaaa ataggttca gctatttgg aaatgcttt 
      tcaatttaa gtttttaat gtaaaacct tgaaaaaag 
      ttttcagcc caaaaatgt tctacaaag ctcgaaaaa 
      ttataaagt gcatcactt ttgacgcaa aataatgtt 
      acttttttt ttaaagaag cgggaaata aatatattt 
      ccgacttaa aaaactaaa accggatga aatatttgg 
      aaatcaaag acataattt tattttcga cgcaaaaat 
      ctaatttta tgttaaaaa aatctagag aaaaataaa 
      aagttattg tttgaaaca tctgaaaaa tgtaaaaat 
      aacattata tttttgaaa cgagttagt gatttttca 
      gcctaccat aaagtaaat aaatattta gctccaaag 
      ggccaaggt taaaaagtt tatttcgtt gaaaaacgt 
      cataatacc cgaaagaac aaagttatt ttcaagcaa 
      attttgtaa aaaatgcaa ttttgagga aaaattagt 
      aacgttttt aatctaaac atgtgtatc tggttcaaa 
      aataaactg aaaatgttc ga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY