Status
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Gene information
General information
TC002466 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 28035613..28038960
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002466
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03380

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002466 RA
      MYSLLVLLS TTLVLTHSQ NCGYNSCPQ WDPNALNVH 
      LVPHSHDDL GWLKTVDKY YFQDVQNVI GSVIGALKQ 
      NPDRKFIQV ETAFFKKWW DHQNDLVKR DVIKLVNNG 
      QLEISNGGW SMNDEATVN YQSTIDQYT LGLRYLEDT 
      LGPCARPKS AWAIDTFGH SREQASIVA QLGFDSTFF 
      MRLDYRDRE KRLKEKTAD LLWQGSQNL NNSNIFTSV 
      FYRGTYSYP DGFCFDIVC NDEPIIDDA NSPDYNYEE 
      KVDDFADFV RGQADKYPT NNIFVAMGD DVRYQAAWT 
      NFLNIDRLI KGFELFPRN YTNKPIKLL YSSPACYTK 
      AVNDYVTAN NYKLEVKTD DFLPYATDG YGYWSGFYV 
      SRPATKRFE RIGNNFLQI AKQIAAVSN QPYENKITP 
      LKEAMGVIQ HHDAITGTE KQDVLKDYH RMLYSAIEE 
      ANELVDPLL STIISGTDD HFQFKTCLM SNISSCSPS 
      RSNKFTVAV YNPLSRVVS SPIEIPVSA KAWSIVDPD 
      GNEVANQVE STIIDFSYV NNVPTSPYT CYFIAKDLP 
      PLGYKIFTF TKVESRRSE LTFGNSNTS FEIDEDSGL 
      IKSITMNGV TMDVSQDLY YYKSGSYSG AYIFVPLVN 
      EKFRVAEGK VKTTPISGS VYQGVVQEF APWAKQIVK 
      VYHGDDSHI EIEWIVGPI DLVSENVGK EVVSVFTTS 
      LKTEGNFYT DSNGREMLK RTRNYRPTF DYTNEEPIA 
      GNYHPITSR IVLKDEEKA LELAVLNDR AQGGTSLKD 
      GQVEIMIQR NCPHNDFGR VGDSINDQE YGQGVITRG 
      RHYIVLGPT SGNGVKSLA AIQRDVAQR KLLPPWAFV 
      TNQEVSQRL KNTQFSSLK VPLSENVQI LTLEPWNNS 
      TILLRLEHI LERNEDENL SQEVTVDLS DLFTTFTIT 
      KLVEMTLGG NIPLEESVR LKWPGLENV QSKKLDGFK 
      VTLAPMQIR TFVATVVY
    CDS sequence
    • >TC002466 RA
      atgtacagt ttacttgtg cttttgagt accacctta 
      gtactcaca cactcgcaa aattgtggt tacaactct 
      tgcccacaa tgggaccct aacgccctc aacgtccac 
      ctagtaccc cactcacac gacgacctc ggttggctc 
      aaaaccgtc gacaagtac tacttccaa gacgtccaa 
      aacgtgatc ggttcagta atcggtgct ttgaagcaa 
      aacccagat cgtaaattc atacaagtt gaaaccgcc 
      tttttcaaa aaatggtgg gaccaccaa aacgattta 
      gtcaaacgg gatgtaatc aaactggtc aataacgga 
      caacttgag ataagcaat ggtggttgg agcatgaat 
      gatgaagct acagttaac taccagtcg acaattgac 
      cagtatacc cttggtcta aggtattta gaagatact 
      ttaggccct tgtgcaaga cctaaatcg gcctgggcc 
      attgatacg ttcggtcat agtagagaa caagcctcc 
      attgtggca caattgggt tttgatagc acgttcttc 
      atgagattg gattacaga gaccgggag aaaagactt 
      aaggaaaaa acggccgat cttttgtgg cagggaagc 
      cagaatttg aacaattcc aacattttc acgtcagtt 
      ttctacaga ggaacgtac agttaccct gatggtttt 
      tgcttcgat atagtttgt aacgacgaa cctattata 
      gatgatgcc aatagtcct gattacaac tacgaggaa 
      aaagtggac gattttgcc gattttgta aggggacag 
      gctgataag taccccact aacaacatt tttgtggca 
      atgggggac gatgtgcgc taccaagcc gcttggacc 
      aattttctc aacattgat agacttatc aaaggtttt 
      gaacttttc ccccgaaat tacaccaac aaaccgatc 
      aaactactc tactccagt ccagcgtgt tacaccaaa 
      gcggtcaac gactacgtt acggcaaat aattacaaa 
      ttggaagtc aaaactgat gattttctt ccatacgcc 
      actgatggt tacggctac tggagcggt ttttacgtc 
      tctagacca gcaaccaag cgatttgag cgaattggt 
      aataacttt cttcaaatt gccaaacaa atagcagct 
      gtcagtaac caaccgtac gaaaataaa atcacacca 
      ctgaaagag gcaatgggt gtgatacag caccatgac 
      gccatcact ggtactgaa aaacaggac gtcctgaaa 
      gactaccac cggatgctt tattcggcc attgaggag 
      gcgaacgag cttgtggac ccacttttg tctaccata 
      ataagtggt accgatgat cattttcaa ttcaaaact 
      tgtctgatg tctaacata agttcgtgt agtccctcg 
      cgctcaaat aaatttact gtggccgtt tacaaccct 
      ttaagtcgg gtcgtttca agcccaatt gaaatacca 
      gtgagcgcc aaagcttgg tcaattgta gaccctgat 
      ggaaacgaa gttgcaaat caagtcgaa tcaacaatc 
      atcgacttc agttacgtc aacaacgtg ccaacttcg 
      ccctacact tgttatttc attgcgaaa gacttgccc 
      cctttgggt tacaaaatt ttcactttt actaaagtg 
      gaatcaaga cgaagcgag ttaactttt ggtaacagt 
      aacaccagt tttgaaatt gacgaagat tcaggtctt 
      attaagtca attactatg aatggggtc acaatggac 
      gtgtctcaa gacctctac tactacaaa agtggcagt 
      tattcgggt gcttacatt tttgttcct ttggttaac 
      gaaaaattt cgagtggcc gaaggtaaa gtcaaaaca 
      acacccatt tcagggagt gtgtaccaa ggggtggtg 
      caagagttt gccccatgg gccaaacaa atcgtcaag 
      gtctaccat ggggatgat agtcatatt gagattgaa 
      tggattgtt ggaccaatt gatttagtt tcggaaaat 
      gttggcaaa gaagttgtt tcggttttt acaacatct 
      ctcaagact gaaggtaat ttttatacg gactccaac 
      ggacgagaa atgttgaaa cgtaccaga aactaccga 
      ccaactttt gattacacc aatgaagag ccgattgct 
      ggcaattac cacccgatc acgtcacga attgtcctc 
      aaagacgaa gaaaaggca ctagaactt gcagtattg 
      aatgacagg gctcaaggt ggtaccagc ctcaaagat 
      ggacaagtt gagattatg atccagaga aattgtcca 
      cataacgat tttggcaga gttggtgat agtatcaac 
      gaccaagaa tacggacaa ggagtgata accagaggg 
      agacattat atagtttta ggcccaact tctgggaac 
      ggagtgaaa agtttggca gcaattcaa agagacgta 
      gcccaaaga aaattatta ccaccatgg gcgttcgtc 
      acaaaccaa gaagtgtcc caacgtctc aagaacaca 
      caatttagc agtcttaag gtaccactc agtgaaaac 
      gtgcaaatt ttgactttg gaaccgtgg aacaatagt 
      acgattctt ctgcgtttg gaacacatt ttagagaga 
      aatgaagac gagaatcta tcgcaggaa gtgaccgtg 
      gatttatcg gatttgttt actacgttc acgatcaca 
      aaactggtt gaaatgact ttggggggc aacattccg 
      ttggaagaa agtgttaga ttaaaatgg ccaggtctg 
      gaaaacgtc caatcgaag aaactagac ggttttaaa 
      gtgactcta gcaccgatg caaattcgc acatttgta 
      gccactgtt gtttattaa
    mRNA sequence
    • >TC002466 RA
      ctccactcc accacaatg tacagttta cttgtgctt 
      ttgagtacc accttagta ctcacacac tcgcaaaat 
      tgtggttac aactcttgc ccacaatgg gaccctaac 
      gccctcaac gtccaccta gtaccccac tcacacgac 
      gacctcggt tggctcaaa accgtcgac aagtactac 
      ttccaagac gtccaaaac gtgatcggt tcagtaatc 
      ggtgctttg aagcaaaac ccagatcgt aaattcata 
      caagttgaa accgccttt ttcaaaaaa tggtgggac 
      caccaaaac gatttagtc aaacgggat gtaatcaaa 
      ctggtcaat aacggacaa cttgagata agcaatggt 
      ggttggagc atgaatgat gaagctaca gttaactac 
      cagtcgaca attgaccag tataccctt ggtctaagg 
      tatttagaa gatacttta ggcccttgt gcaagacct 
      aaatcggcc tgggccatt gatacgttc ggtcatagt 
      agagaacaa gcctccatt gtggcacaa ttgggtttt 
      gatagcacg ttcttcatg agattggat tacagagac 
      cgggagaaa agacttaag gaaaaaacg gccgatctt 
      ttgtggcag ggaagccag aatttgaac aattccaac 
      attttcacg tcagttttc tacagagga acgtacagt 
      taccctgat ggtttttgc ttcgatata gtttgtaac 
      gacgaacct attatagat gatgccaat agtcctgat 
      tacaactac gaggaaaaa gtggacgat tttgccgat 
      tttgtaagg ggacaggct gataagtac cccactaac 
      aacattttt gtggcaatg ggggacgat gtgcgctac 
      caagccgct tggaccaat tttctcaac attgataga 
      cttatcaaa ggttttgaa cttttcccc cgaaattac 
      accaacaaa ccgatcaaa ctactctac tccagtcca 
      gcgtgttac accaaagcg gtcaacgac tacgttacg 
      gcaaataat tacaaattg gaagtcaaa actgatgat 
      tttcttcca tacgccact gatggttac ggctactgg 
      agcggtttt tacgtctct agaccagca accaagcga 
      tttgagcga attggtaat aactttctt caaattgcc 
      aaacaaata gcagctgtc agtaaccaa ccgtacgaa 
      aataaaatc acaccactg aaagaggca atgggtgtg 
      atacagcac catgacgcc atcactggt actgaaaaa 
      caggacgtc ctgaaagac taccaccgg atgctttat 
      tcggccatt gaggaggcg aacgagctt gtggaccca 
      cttttgtct accataata agtggtacc gatgatcat 
      tttcaattc aaaacttgt ctgatgtct aacataagt 
      tcgtgtagt ccctcgcgc tcaaataaa tttactgtg 
      gccgtttac aacccttta agtcgggtc gtttcaagc 
      ccaattgaa ataccagtg agcgccaaa gcttggtca 
      attgtagac cctgatgga aacgaagtt gcaaatcaa 
      gtcgaatca acaatcatc gacttcagt tacgtcaac 
      aacgtgcca acttcgccc tacacttgt tatttcatt 
      gcgaaagac ttgccccct ttgggttac aaaattttc 
      acttttact aaagtggaa tcaagacga agcgagtta 
      acttttggt aacagtaac accagtttt gaaattgac 
      gaagattca ggtcttatt aagtcaatt actatgaat 
      ggggtcaca atggacgtg tctcaagac ctctactac 
      tacaaaagt ggcagttat tcgggtgct tacattttt 
      gttcctttg gttaacgaa aaatttcga gtggccgaa 
      ggtaaagtc aaaacaaca cccatttca gggagtgtg 
      taccaaggg gtggtgcaa gagtttgcc ccatgggcc 
      aaacaaatc gtcaaggtc taccatggg gatgatagt 
      catattgag attgaatgg attgttgga ccaattgat 
      ttagtttcg gaaaatgtt ggcaaagaa gttgtttcg 
      gtttttaca acatctctc aagactgaa ggtaatttt 
      tatacggac tccaacgga cgagaaatg ttgaaacgt 
      accagaaac taccgacca acttttgat tacaccaat 
      gaagagccg attgctggc aattaccac ccgatcacg 
      tcacgaatt gtcctcaaa gacgaagaa aaggcacta 
      gaacttgca gtattgaat gacagggct caaggtggt 
      accagcctc aaagatgga caagttgag attatgatc 
      cagagaaat tgtccacat aacgatttt ggcagagtt 
      ggtgatagt atcaacgac caagaatac ggacaagga 
      gtgataacc agagggaga cattatata gttttaggc 
      ccaacttct gggaacgga gtgaaaagt ttggcagca 
      attcaaaga gacgtagcc caaagaaaa ttattacca 
      ccatgggcg ttcgtcaca aaccaagaa gtgtcccaa 
      cgtctcaag aacacacaa tttagcagt cttaaggta 
      ccactcagt gaaaacgtg caaattttg actttggaa 
      ccgtggaac aatagtacg attcttctg cgtttggaa 
      cacatttta gagagaaat gaagacgag aatctatcg 
      caggaagtg accgtggat ttatcggat ttgtttact 
      acgttcacg atcacaaaa ctggttgaa atgactttg 
      gggggcaac attccgttg gaagaaagt gttagatta 
      aaatggcca ggtctggaa aacgtccaa tcgaagaaa 
      ctagacggt tttaaagtg actctagca ccgatgcaa 
      attcgcaca tttgtagcc actgttgtt tattaaatt 
      atctaactt ttgtttgca aataaagat aagattcta 
      aa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY