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Gene information
General information
TC002459 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid29: 1356442..1358149
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002459
    iBeetle screen iB_03378
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%

    cuticle remnants strongly fragmented (cuticle crumbs) - Penetrance (percentage of animals/eggs on slide): less than 30%

    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    procephalic appendages partiallyremaining - Penetrance (percentage of animals/eggs on slide): less than 30%
    gnathal appendages partiallyremaining - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen partiallyeverted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%
    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    head irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen sometimes partiallyeverted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002459 RA
      MSRYGLTSL LLKLNKLVP HSSQANQTS MSQIKTFVF 
      LDLETTGLP HLEHNKTRI TELCAVSVR ADHIELGCF 
      PRVQNKLSL CFNPMKMIS PEATSLTGL SNDLLEYQA 
      SFSSDAVDV IRKWLEMNQ KPICLVAHN GNRFDYPIL 
      RAEVEKTRN KLPEDILCL DSLNAFRSI DTLEAEAVK 
      QPPKGEIPF EFNDGYDEL LSQAVDEYE KQMKLTPEQ 
      VQKINETTP QKQKICSTT GNGGPGAST KRNFGHIRE 
      FVSYKLEDV YKRLTSKEP ISAHEAEGD VTMLVTCAA 
      VYGEKFVKY ANDNSKRLS EIPAMTPGR RIGF
    • >TC002459 RB
      MTTGTGSTD HLLNLSNLC RQTVACVTP SQKDLETTG 
      LPHLEHNKT RITELCAVS VRADHIELG CFPRVQNKL 
      SLCFNPMKM ISPEATSLT GLSNDLLEY QASFSSDAV 
      DVIRKWLEM NQKPICLVA HNGNRFDYP ILRAEVEKT 
      RNKLPEDIL CLDSLNAFR SIDTLEAEA VKQPPKGEI 
      PFEFNDGYD ELLSQAVDE YEKQMKLTP EQVQKINET 
      TPQKQKICS TTGNGGPGA STKRNFGHI REFVSYKLE 
      DVYKRLTSK EPISAHEAE GDVTMLVTC AAVYGEKFV 
      KYANDNSKR LSEIPAMTP GRRIGF
    CDS sequence
    • >TC002459 RA
      atgagcaga tatggtctc actagccta ctgttaaaa 
      ctgaacaag ttggtgccc cactcgagt caagccaac 
      caaacctca atgtctcaa atcaaaacg ttcgttttc 
      ctagacctg gagaccacc ggtttgccc catttggag 
      cacaacaag acccgaatt accgaatta tgtgccgtg 
      tcggtccga gcggaccac atcgagctc gggtgcttc 
      ccccgagtt caaaataaa ttgtccttg tgtttcaac 
      cctatgaag atgattagt cccgaagcc acctccctg 
      acagggctc tcgaacgac ttgttggag taccaggcg 
      agtttttcg agtgacgca gtggacgtg ataaggaag 
      tggttagag atgaatcag aaaccgatt tgtttggtt 
      gcacataac gggaatcga tttgattat ccaattctg 
      agagcggag gtcgaaaaa acgcggaat aaattacca 
      gaggatatt ctctgtctt gactctctt aacgcattt 
      agaagcatt gatacgtta gaggcagag gcggtgaaa 
      cagccaccc aaaggagaa ataccgttt gagtttaac 
      gacggttat gatgagctt ttaagccaa gcagtggac 
      gagtatgaa aagcaaatg aaactcaca cctgaacaa 
      gtacaaaaa attaacgaa acgacgcca caaaaacaa 
      aagatttgc tcaacaact ggaaatggg ggccctggc 
      gcttcaacc aaacgtaat tttgggcac attagggaa 
      ttcgttagt tataaattg gaagatgtt tacaaaaga 
      ctgacttct aaagaacca atcagtgca catgaggcc 
      gaaggcgat gttacgatg ctagtcact tgtgcggcg 
      gtttatggc gaaaaattc gtcaaatat gcaaacgat 
      aattcgaaa agactgtca gaaattcca gccatgacg 
      cccggcaga agaattgga ttttaa
    • >TC002459 RB
      atgacgacc ggcaccggc agcacagat cacttactt 
      aacctctct aatctgtgc cggcagaca gtggcgtgc 
      gtcacgccc tcccagaaa gacctggag accaccggt 
      ttgccccat ttggagcac aacaagacc cgaattacc 
      gaattatgt gccgtgtcg gtccgagcg gaccacatc 
      gagctcggg tgcttcccc cgagttcaa aataaattg 
      tccttgtgt ttcaaccct atgaagatg attagtccc 
      gaagccacc tccctgaca gggctctcg aacgacttg 
      ttggagtac caggcgagt ttttcgagt gacgcagtg 
      gacgtgata aggaagtgg ttagagatg aatcagaaa 
      ccgatttgt ttggttgca cataacggg aatcgattt 
      gattatcca attctgaga gcggaggtc gaaaaaacg 
      cggaataaa ttaccagag gatattctc tgtcttgac 
      tctcttaac gcatttaga agcattgat acgttagag 
      gcagaggcg gtgaaacag ccacccaaa ggagaaata 
      ccgtttgag tttaacgac ggttatgat gagctttta 
      agccaagca gtggacgag tatgaaaag caaatgaaa 
      ctcacacct gaacaagta caaaaaatt aacgaaacg 
      acgccacaa aaacaaaag atttgctca acaactgga 
      aatgggggc cctggcgct tcaaccaaa cgtaatttt 
      gggcacatt agggaattc gttagttat aaattggaa 
      gatgtttac aaaagactg acttctaaa gaaccaatc 
      agtgcacat gaggccgaa ggcgatgtt acgatgcta 
      gtcacttgt gcggcggtt tatggcgaa aaattcgtc 
      aaatatgca aacgataat tcgaaaaga ctgtcagaa 
      attccagcc atgacgccc ggcagaaga attggattt 
      taa
    mRNA sequence
    • >TC002459 RA
      gcagacagt ggcgtgcgt cacgccctc ccagaaagg 
      taaacaaca actttccct cttaattgc gctcaatta 
      gcaggcact tatgactac attacgata ttaagaata 
      tattaagaa tgagcagat atggtctca ctagcctac 
      tgttaaaac tgaacaagt tggtgcccc actcgagtc 
      aagccaacc aaacctcaa tgtctcaaa tcaaaacgt 
      tcgttttcc tagacctgg agaccaccg gtttgcccc 
      atttggagc acaacaaga cccgaatta ccgaattat 
      gtgccgtgt cggtccgag cggaccaca tcgagctcg 
      ggtgcttcc cccgagttc aaaataaat tgtccttgt 
      gtttcaacc ctatgaaga tgattagtc ccgaagcca 
      cctccctga cagggctct cgaacgact tgttggagt 
      accaggcga gtttttcga gtgacgcag tggacgtga 
      taaggaagt ggttagaga tgaatcaga aaccgattt 
      gtttggttg cacataacg ggaatcgat ttgattatc 
      caattctga gagcggagg tcgaaaaaa cgcggaata 
      aattaccag aggatattc tctgtcttg actctctta 
      acgcattta gaagcattg atacgttag aggcagagg 
      cggtgaaac agccaccca aaggagaaa taccgtttg 
      agtttaacg acggttatg atgagcttt taagccaag 
      cagtggacg agtatgaaa agcaaatga aactcacac 
      ctgaacaag tacaaaaaa ttaacgaaa cgacgccac 
      aaaaacaaa agatttgct caacaactg gaaatgggg 
      gccctggcg cttcaacca aacgtaatt ttgggcaca 
      ttagggaat tcgttagtt ataaattgg aagatgttt 
      acaaaagac tgacttcta aagaaccaa tcagtgcac 
      atgaggccg aaggcgatg ttacgatgc tagtcactt 
      gtgcggcgg tttatggcg aaaaattcg tcaaatatg 
      caaacgata attcgaaaa gactgtcag aaattccag 
      ccatgacgc ccggcagaa gaattggat tttaatgat 
      tttatcatt tttttattt tatttatac tgactaata 
      tgtgtgtta tg
    • >TC002459 RB
      taaatttta aataaatta atcaaaatg acgaccggc 
      accggcagc acagatcac ttacttaac ctctctaat 
      ctgtgccgg cagacagtg gcgtgcgtc acgccctcc 
      cagaaagac ctggagacc accggtttg ccccatttg 
      gagcacaac aagacccga attaccgaa ttatgtgcc 
      gtgtcggtc cgagcggac cacatcgag ctcgggtgc 
      ttcccccga gttcaaaat aaattgtcc ttgtgtttc 
      aaccctatg aagatgatt agtcccgaa gccacctcc 
      ctgacaggg ctctcgaac gacttgttg gagtaccag 
      gcgagtttt tcgagtgac gcagtggac gtgataagg 
      aagtggtta gagatgaat cagaaaccg atttgtttg 
      gttgcacat aacgggaat cgatttgat tatccaatt 
      ctgagagcg gaggtcgaa aaaacgcgg aataaatta 
      ccagaggat attctctgt cttgactct cttaacgca 
      tttagaagc attgatacg ttagaggca gaggcggtg 
      aaacagcca cccaaagga gaaataccg tttgagttt 
      aacgacggt tatgatgag cttttaagc caagcagtg 
      gacgagtat gaaaagcaa atgaaactc acacctgaa 
      caagtacaa aaaattaac gaaacgacg ccacaaaaa 
      caaaagatt tgctcaaca actggaaat gggggccct 
      ggcgcttca accaaacgt aattttggg cacattagg 
      gaattcgtt agttataaa ttggaagat gtttacaaa 
      agactgact tctaaagaa ccaatcagt gcacatgag 
      gccgaaggc gatgttacg atgctagtc acttgtgcg 
      gcggtttat ggcgaaaaa ttcgtcaaa tatgcaaac 
      gataattcg aaaagactg tcagaaatt ccagccatg 
      acgcccggc agaagaatt ggattttaa tgattttat 
      cattttttt attttattt atactgact aatatgtgt 
      gttatg