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Gene information
General information
TC002431 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 28958007..28962764
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002431
    iBeetle screen iB_03375

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002431 RA
      MGNTRSAPE HEEDVWWSN NKSSVDNKS VEVVEPIEE 
      TPADRQQRH KEELDEFKL ELARKHEKR RQIIAEKRK 
      EMQDLRDEV KRLTQENEE LKKTCGPND LEEIKRNNL 
      ELKMTIATL QKDIQNLNS EVVDFEKER QDYKAHVVA 
      LKDVVSVSK QMLMIREAQ LKEMREKIK EIEASLAGK 
      ELKVLSQDL KAEYEKQLQ NIRNLRIFY EERQRADKK 
      QKEEMKKLL EEVKIDLQD EKNKNSELE ENLARLEED 
      NSKKYDEIK SLESNLGLT KAECRQYQA ELSVINQLF 
      SQILLGFKN NQEIDIDKL IKLLEENHH LLQDIAVNE 
      ESNQVSALP KVLLDLVSE VSDSKDLEE EEEASKTNP 
      ETPNQVNSA SEIVENLPK VWKVLIELL SHQSAPTTC 
      DNSTSENPC YKVVEGPKG PHLVLSVSQ TFIRLKNLI 
      MEKKSLERE TNRLKQLNT HLEGRLQEQ EKRLELVSH 
      ELSETWHVV GKLQKKHEL LHTQEKILR YELAQKRKL 
      LTELKGELE YSREKWQEA REKNSNTEK QWEQLRIEF 
      ASRKNGGGD DSNNSIESG YSDDKESSS EDDFEEKTT 
      DAREERLLR LESQCSSLV KQVTSTTSR GEAMVSKLD 
      ALHATYGEG REEGETSDG RGSE
    CDS sequence
    • >TC002431 RA
      atgggtaac acgcgcagt gcaccagaa cacgaagag 
      gatgtgtgg tggagtaac aacaaatct tcagttgat 
      aataaaagt gtggaagtt gttgaacca attgaagaa 
      actccagcc gatcgtcag caacgccac aaggaagaa 
      ctggacgag ttcaagcta gaactagcg cgaaagcac 
      gagaaacgt agacagata atcgccgag aaacggaaa 
      gaaatgcag gatttgcgt gacgaagtc aagcgtttg 
      acgcaagaa aacgaagaa ttgaagaaa acatgcggt 
      ccgaacgac ttggaagaa atcaaacgg aataatctc 
      gaattgaag atgacaatc gcgacacta cagaaggac 
      atccaaaat ttgaacagt gaagttgtg gattttgaa 
      aaggagaga caggattac aaggcccac gttgtcgct 
      ctgaaggac gttgtgagt gtttccaag caaatgttg 
      atgattcga gaggcgcaa cttaaagaa atgcgcgaa 
      aaaatcaaa gaaattgaa gcctcgttg gctggaaag 
      gaactgaag gttttgtcg caggacttg aaggctgag 
      tacgaaaaa cagctgcag aatatccgt aacttgcgg 
      attttctac gaagagcgg caacgcgcc gataagaaa 
      cagaaggaa gaaatgaag aaactgcta gaagaggtg 
      aagattgat ttacaagac gagaagaac aaaaattcc 
      gagcttgag gagaatttg gccaggctt gaggaagat 
      aattcgaag aaatacgac gagatcaaa agtcttgaa 
      tcaaatttg ggtttgacc aaagctgag tgtcgccaa 
      taccaggcc gaactgtcc gttataaat caattgttc 
      tcccaaatc ctccttggt ttcaaaaac aaccaagag 
      atcgacatc gacaaactc atcaagctg ttggaggag 
      aaccatcac ctcctccag gacattgcc gtgaacgag 
      gagtccaat caagtgtca gctctcccg aaggttttg 
      ttggaccta gtgagcgaa gtgagcgac tccaaggac 
      ttggaggag gaagaagaa gcgtcaaaa acaaacccg 
      gaaacgccc aatcaggtg aacagcgcg tctgaaatt 
      gttgaaaat ttgccaaaa gtgtggaaa gtcctgatt 
      gagcttttg agtcatcag agtgccccc acgacctgt 
      gacaactcc acgagtgaa aacccctgc tacaaggtg 
      gtggagggg ccaaagggc ccccacttg gtcttgagc 
      gtcagccag acctttatc cgactgaag aacttgatt 
      atggagaaa aagtcgctc gagcgtgaa accaaccgc 
      ctcaagcag ctcaacacg cacctggag ggccgcctc 
      caagagcag gagaagcgc ctggagctg gtctcgcac 
      gagctctcc gagacctgg cacgtggtg ggcaaactc 
      cagaagaag cacgaactc ctccacacg caagaaaaa 
      atcctccgg tacgaactt gcccaaaaa cgcaaactc 
      ttgaccgaa ctcaaaggc gagctggaa tacagccga 
      gaaaagtgg caagaagcc agagagaaa aactccaat 
      acggaaaaa caatgggaa cagttgcga atcgagttc 
      gcttcgcgt aaaaacgga ggaggcgat gattccaac 
      aacagcatc gagagtggc tacagtgac gataaagaa 
      tcttcgagt gaagatgat ttcgaagaa aagacaact 
      gacgcacgt gaagaaaga ttgctgcgc ttggaaagc 
      caatgcagt agcttggtg aaacaagtg acgagcacg 
      acgagtaga ggggaggcc atggtcagc aaactagat 
      gcgttgcat gcgacgtac ggggagggg cgagaggag 
      ggggaaacg tccgatggg cggggctcc gagtaa
    mRNA sequence
    • >TC002431 RA
      agccagtgc gaattttag tattatata gtttagttc 
      gtgtaagtt tccatcttg ctaaacgtc ttagtcaca 
      atgggtaac acgcgcagt gcaccagaa cacgaagag 
      gatgtgtgg tggagtaac aacaaatct tcagttgat 
      aataaaagt gtggaagtt gttgaacca attgaagaa 
      actccagcc gatcgtcag caacgccac aaggaagaa 
      ctggacgag ttcaagcta gaactagcg cgaaagcac 
      gagaaacgt agacagata atcgccgag aaacggaaa 
      gaaatgcag gatttgcgt gacgaagtc aagcgtttg 
      acgcaagaa aacgaagaa ttgaagaaa acatgcggt 
      ccgaacgac ttggaagaa atcaaacgg aataatctc 
      gaattgaag atgacaatc gcgacacta cagaaggac 
      atccaaaat ttgaacagt gaagttgtg gattttgaa 
      aaggagaga caggattac aaggcccac gttgtcgct 
      ctgaaggac gttgtgagt gtttccaag caaatgttg 
      atgattcga gaggcgcaa cttaaagaa atgcgcgaa 
      aaaatcaaa gaaattgaa gcctcgttg gctggaaag 
      gaactgaag gttttgtcg caggacttg aaggctgag 
      tacgaaaaa cagctgcag aatatccgt aacttgcgg 
      attttctac gaagagcgg caacgcgcc gataagaaa 
      cagaaggaa gaaatgaag aaactgcta gaagaggtg 
      aagattgat ttacaagac gagaagaac aaaaattcc 
      gagcttgag gagaatttg gccaggctt gaggaagat 
      aattcgaag aaatacgac gagatcaaa agtcttgaa 
      tcaaatttg ggtttgacc aaagctgag tgtcgccaa 
      taccaggcc gaactgtcc gttataaat caattgttc 
      tcccaaatc ctccttggt ttcaaaaac aaccaagag 
      atcgacatc gacaaactc atcaagctg ttggaggag 
      aaccatcac ctcctccag gacattgcc gtgaacgag 
      gagtccaat caagtgtca gctctcccg aaggttttg 
      ttggaccta gtgagcgaa gtgagcgac tccaaggac 
      ttggaggag gaagaagaa gcgtcaaaa acaaacccg 
      gaaacgccc aatcaggtg aacagcgcg tctgaaatt 
      gttgaaaat ttgccaaaa gtgtggaaa gtcctgatt 
      gagcttttg agtcatcag agtgccccc acgacctgt 
      gacaactcc acgagtgaa aacccctgc tacaaggtg 
      gtggagggg ccaaagggc ccccacttg gtcttgagc 
      gtcagccag acctttatc cgactgaag aacttgatt 
      atggagaaa aagtcgctc gagcgtgaa accaaccgc 
      ctcaagcag ctcaacacg cacctggag ggccgcctc 
      caagagcag gagaagcgc ctggagctg gtctcgcac 
      gagctctcc gagacctgg cacgtggtg ggcaaactc 
      cagaagaag cacgaactc ctccacacg caagaaaaa 
      atcctccgg tacgaactt gcccaaaaa cgcaaactc 
      ttgaccgaa ctcaaaggc gagctggaa tacagccga 
      gaaaagtgg caagaagcc agagagaaa aactccaat 
      acggaaaaa caatgggaa cagttgcga atcgagttc 
      gcttcgcgt aaaaacgga ggaggcgat gattccaac 
      aacagcatc gagagtggc tacagtgac gataaagaa 
      tcttcgagt gaagatgat ttcgaagaa aagacaact 
      gacgcacgt gaagaaaga ttgctgcgc ttggaaagc 
      caatgcagt agcttggtg aaacaagtg acgagcacg 
      acgagtaga ggggaggcc atggtcagc aaactagat 
      gcgttgcat gcgacgtac ggggagggg cgagaggag 
      ggggaaacg tccgatggg cggggctcc gagtaaagt 
      acaaacttt tgtacaaaa caag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY