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Gene information
General information
TC002422 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 28747228..28749571
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002422
    iBeetle screen iB_03373

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002422 RA
      MSSNRPYTS CIKIRGTPI IPPLVTQKV REECLEYKR 
      KAIELEKKH ERNRLFQQC LVDLERLQD KTKDLTIPK 
      QTTLENQLT FVEDSSVHS SSPVPYDTY KKLQNLTSE 
      TIDVSEYSD EGVKVMGNE ERVTPPKLI RSNSYTLES 
      PSPILLAHL EKEARKCIE MEENESRSL SPITVIDSE 
      REPPLQVVD TDVSVQQVT VTTVEPQLR EILDKLPQE 
      HARNILEIL NKQQEEQKS VTEKFTTPD KSMTVSISS 
      QSLYYSVLS SETTPHGSP LNVVNLRNA KNMDKDRAA 
      SVIGAAVRG YLVRRLIRT EKVQGLIDT IKDALLCAI 
      QLHNAEVIE PADVELHRR LIQQVSAAC YAFHDIFFT 
      LSTKEQMGI IAMDRQRKI EKANRPLSA ASSNLSISR 
      RQSARMVR
    CDS sequence
    • >TC002422 RA
      atgtcatca aatcgtccc tacacttcg tgtatcaaa 
      atcaggggc accccaata atacctcct ttggtcaca 
      caaaaagtc cgtgaggaa tgcctcgaa tacaaacga 
      aaagcaatc gagttagaa aaaaagcac gaaaggaac 
      cgacttttc cagcaatgt ttagtcgac cttgagagg 
      ctccaagac aaaaccaaa gacctgaca ataccgaag 
      cagaccacc cttgagaat caattgact tttgttgag 
      gattcttca gttcattca tctagtccc gtgccttac 
      gacacttac aaaaaacta cagaactta acaagtgag 
      actatcgat gttagtgag tacagtgat gagggggtg 
      aaagtgatg ggtaacgag gaacgagtc acccctcca 
      aagttgatt cggagcaac tcgtacact ttggagagc 
      cccagcccc attcttttg gcccatttg gagaaagag 
      gcccgcaaa tgcatagaa atggaagag aatgagtct 
      cggagcttg tctccaatc acggtgatc gacagcgag 
      cgggagccc ccgttacag gtcgtcgac acggacgtt 
      tcagttcag caggtcact gtgacgaca gtggagccc 
      caactgcgg gaaatcctg gataaactg ccccaagaa 
      cacgcccga aacatcctc gaaattttg aataagcaa 
      caggaggag cagaaaagt gtgactgaa aaatttaca 
      acccctgac aaatcaatg actgtttca atctcaagc 
      cagtccctt tactactca gtattatcg tcggagacg 
      acgccacat ggcagcccc ctcaacgtt gtcaacctc 
      cgtaatgcg aaaaatatg gacaaggac agggcggcg 
      agtgtgata ggggctgca gtgaggggt tatttagtt 
      aggcgactt atcaggact gaaaaggtg caagggctg 
      attgatacg ataaaagac gcccttttg tgcgccatc 
      caattacac aatgccgaa gtgattgaa cctgccgat 
      gtggagctc caccgacga ctgattcag caagtttca 
      gctgcgtgt tatgcattc catgacatt tttttcaca 
      ctttcgacc aaagaacaa atggggatt attgctatg 
      gacaggcaa cggaaaatc gaaaaggcc aataggcct 
      ctctcagcg gcaagctca aacttgtcc atttcgcgg 
      agacaaagc gctcgcatg gtgcgatga
    mRNA sequence
    • >TC002422 RA
      acaaacata tcaaaaata actacagcc gccgtttat 
      gggtttctt aacaacgct ttgggccct gaaatgtca 
      tcaaatcgt ccctacact tcgtgtatc aaaatcagg 
      ggcacccca ataatacct cctttggtc acacaaaaa 
      gtccgtgag gaatgcctc gaatacaaa cgaaaagca 
      atcgagtta gaaaaaaag cacgaaagg aaccgactt 
      ttccagcaa tgtttagtc gaccttgag aggctccaa 
      gacaaaacc aaagacctg acaataccg aagcagacc 
      acccttgag aatcaattg acttttgtt gaggattct 
      tcagttcat tcatctagt cccgtgcct tacgacact 
      tacaaaaaa ctacagaac ttaacaagt gagactatc 
      gatgttagt gagtacagt gatgagggg gtgaaagtg 
      atgggtaac gaggaacga gtcacccct ccaaagttg 
      attcggagc aactcgtac actttggag agccccagc 
      cccattctt ttggcccat ttggagaaa gaggcccgc 
      aaatgcata gaaatggaa gagaatgag tctcggagc 
      ttgtctcca atcacggtg atcgacagc gagcgggag 
      cccccgtta caggtcgtc gacacggac gtttcagtt 
      cagcaggtc actgtgacg acagtggag ccccaactg 
      cgggaaatc ctggataaa ctgccccaa gaacacgcc 
      cgaaacatc ctcgaaatt ttgaataag caacaggag 
      gagcagaaa agtgtgact gaaaaattt acaacccct 
      gacaaatca atgactgtt tcaatctca agccagtcc 
      ctttactac tcagtatta tcgtcggag acgacgcca 
      catggcagc cccctcaac gttgtcaac ctccgtaat 
      gcgaaaaat atggacaag gacagggcg gcgagtgtg 
      ataggggct gcagtgagg ggttattta gttaggcga 
      cttatcagg actgaaaag gtgcaaggg ctgattgat 
      acgataaaa gacgccctt ttgtgcgcc atccaatta 
      cacaatgcc gaagtgatt gaacctgcc gatgtggag 
      ctccaccga cgactgatt cagcaagtt tcagctgcg 
      tgttatgca ttccatgac atttttttc acactttcg 
      accaaagaa caaatgggg attattgct atggacagg 
      caacggaaa atcgaaaag gccaatagg cctctctca 
      gcggcaagc tcaaacttg tccatttcg cggagacaa 
      agcgctcgc atggtgcga tgaccaagt ggcttatta 
      aagacaagc aatgatata tttattttt attatacta 
      tatagcgac tatgactaa ataaagtag tattttatg 
      a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY