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Gene information
General information
TC002393 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 31340935..31346252
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002393
    iBeetle screen iB_03368

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002393 RA
      MRRQQQSQS QLQIEHSDD DCTSSQSQI LERVQSDRV 
      TVTKPEEDR RRRTIIVEK KNGSYGFTL QSYGIHYKK 
      EQEIEMITY VDYVDYDGP AYRAGMREG DVILSINGT 
      DMEKADHKT LVNFIKNCD TRMRMVVLF EDCVRKVEL 
      HMRYIQLQR VLQSKMTEL EKLCIRERQ LLEGKWKTH 
      SLPARKKAS QSSNNGDPP TPTQAAYSY CRPTVSTED 
      VAKVQQKQQ NPPLIFAYQ YLDPHYRYM LQPSASSSG 
      EYLLTLEPS RYRGDQHHF IVKTPCDAP QKQNRGNQT 
      NGTETKPKK VQSGHLCNP CMQSANNTD NTSLEAYDL 
      ASPCCDPHC VPSSRRRSR KDHRKRDSK TEETQTDPQ 
      QRSRPHSNT QQQTFSVPR RYFHFGTGL VSQCSLHSC 
      TSSELSAVA PTTMGESST SYTTSLSTD TLYWDEMSA 
      SRQMSLKSA SKHDHQQRY TQTHSHDPV YVHYTTVKP 
      KSWDNLTTK AFGGYGFGY GYLDTKCPK QRSQKTQYV 
      RVDKHTQPQ YTPHTHRRY FQPTKSTES LLSVPKYSN 
      EALSDSSEC LEGSSPAPD ASEGRFFQS PRQSISVSP 
      TDPNFGYYS ARRPSKNVV STSSEATRL
    CDS sequence
    • >TC002393 RA
      atgaggaga caacagcaa agccaatcg caattacaa 
      atcgaacac agcgatgat gactgcact tcatcgcag 
      tctcagatt ttggagcgc gtgcaatcg gatcgagtg 
      actgtaaca aaaccggaa gaagatcgt cgaagacgc 
      acaataatc gttgaaaaa aagaatggt tcgtatggt 
      tttacactt caaagttat ggaatccac tacaagaaa 
      gagcaagaa atcgaaatg attacgtat gtggattat 
      gtggattat gatgggccc gcttacagg gccggaatg 
      agggaaggt gatgtcatt ttatcaatt aatggcaca 
      gatatggag aaagctgat cataaaact ttggtgaat 
      tttatcaaa aattgcgat acaaggatg cggatggtt 
      gtgctgttt gaagactgt gtccggaag gtggagttg 
      cacatgcga tacattcag ctccagcga gtcctccag 
      agtaaaatg accgaattg gaaaaatta tgcatacgt 
      gaaagacag cttttggag ggcaaatgg aagactcat 
      agtttgcca gctcgtaaa aaagcctcg caaagtagc 
      aacaacggg gatcccccg actccaacc caagccgcc 
      tattcctac tgtcgacca actgtatca accgaagac 
      gtggcaaaa gtccagcaa aaacaacaa aatccccct 
      ttaatattc gcctatcaa tacttagac ccccactat 
      cgctacatg ctccagcct tcggcttcc agcagcggc 
      gaatatctg ctaactctg gaaccgtcg cgctaccga 
      ggagaccaa caccatttc atcgtgaaa acaccatgt 
      gatgcacca cagaagcaa aaccgcggc aatcaaacc 
      aacggaacc gaaaccaaa ccgaaaaaa gtccaatct 
      ggccacttg tgcaaccct tgcatgcaa tcagcaaat 
      aacacagat aacaccagt ttggaagcg tacgatttg 
      gcgagtccg tgctgtgat ccacactgt gtgccatca 
      agtagacgc aggtcgcgc aaagaccac cgaaaaaga 
      gattcaaag actgaggaa acacaaact gaccctcaa 
      caacgatct cggccacat tccaacaca caacagcaa 
      actttctca gtcccgagg aggtatttc cactttggg 
      accggacta gtgagtcaa tgtagtctc cattcgtgc 
      acttcgagc gagttgagc gctgtggca cccaccact 
      atgggggag agctccacc agctacacc acctccttg 
      agcaccgat acgctctat tgggatgaa atgagtgcc 
      tcgaggcag atgagtctc aagtcggcc tccaaacac 
      gaccaccaa cagagatac acccagacg cactcccat 
      gaccccgtc tacgtccac tacaccacc gtcaagccg 
      aaatcgtgg gacaacctc accacgaaa gccttcggc 
      ggctacggc ttcggctac ggctacttg gacaccaag 
      tgtcccaaa cagcgctcc caaaaaacg caatacgtc 
      cgagtggac aaacacacc cagccccag tacacccca 
      cacacccac cgacgctac ttccagccc accaagtcc 
      acagagagt ctcctctca gtgccaaag tactccaat 
      gaggctttg tccgattcc agcgaatgt ctggagggc 
      tcttcacca gctccggac gcctccgag ggccgattt 
      ttccaaagt ccgagacag agcatcagt gtgagcccc 
      actgacccc aactttggc tactacagt gccaggagg 
      ccctccaaa aatgtagtc tcgaccagt tcggaggct 
      actagattg taa
    mRNA sequence
    • >TC002393 RA
      cgacgcttg gcgtcatgg tggccgtcg ttatcgaac 
      agctgtttc caaaatggt tttagtcgt gcgagaatg 
      agtcgtgtg tgtgaaaat gaggagaca acagcaaag 
      ccaatcgca attacaaat cgaacacag cgatgatga 
      ctgcacttc atcgcagtc tcagatttt ggagcgcgt 
      gcaatcgga tcgagtgac tgtaacaaa accggaaga 
      agatcgtcg aagacgcac aataatcgt tgaaaaaaa 
      gaatggttc gtatggttt tacacttca aagttatgg 
      aatccacta caagaaaga gcaagaaat cgaaatgat 
      tacgtatgt ggattatgt ggattatga tgggcccgc 
      ttacagggc cggaatgag ggaaggtga tgtcatttt 
      atcaattaa tggcacaga tatggagaa agctgatca 
      taaaacttt ggtgaattt tatcaaaaa ttgcgatac 
      aaggatgcg gatggttgt gctgtttga agactgtgt 
      ccggaaggt ggagttgca catgcgata cattcagct 
      ccagcgagt cctccagag taaaatgac cgaattgga 
      aaaattatg catacgtga aagacagct tttggaggg 
      caaatggaa gactcatag tttgccagc tcgtaaaaa 
      agcctcgca aagtagcaa caacgggga tcccccgac 
      tccaaccca agccgccta ttcctactg tcgaccaac 
      tgtatcaac cgaagacgt ggcaaaagt ccagcaaaa 
      acaacaaaa tcccccttt aatattcgc ctatcaata 
      cttagaccc ccactatcg ctacatgct ccagccttc 
      ggcttccag cagcggcga atatctgct aactctgga 
      accgtcgcg ctaccgagg agaccaaca ccatttcat 
      cgtgaaaac accatgtga tgcaccaca gaagcaaaa 
      ccgcggcaa tcaaaccaa cggaaccga aaccaaacc 
      gaaaaaagt ccaatctgg ccacttgtg caacccttg 
      catgcaatc agcaaataa cacagataa caccagttt 
      ggaagcgta cgatttggc gagtccgtg ctgtgatcc 
      acactgtgt gccatcaag tagacgcag gtcgcgcaa 
      agaccaccg aaaaagaga ttcaaagac tgaggaaac 
      acaaactga ccctcaaca acgatctcg gccacattc 
      caacacaca acagcaaac tttctcagt cccgaggag 
      gtatttcca ctttgggac cggactagt gagtcaatg 
      tagtctcca ttcgtgcac ttcgagcga gttgagcgc 
      tgtggcacc caccactat gggggagag ctccaccag 
      ctacaccac ctccttgag caccgatac gctctattg 
      ggatgaaat gagtgcctc gaggcagat gagtctcaa 
      gtcggcctc caaacacga ccaccaaca gagatacac 
      ccagacgca ctcccatga ccccgtcta cgtccacta 
      caccaccgt caagccgaa atcgtggga caacctcac 
      cacgaaagc cttcggcgg ctacggctt cggctacgg 
      ctacttgga caccaagtg tcccaaaca gcgctccca 
      aaaaacgca atacgtccg agtggacaa acacaccca 
      gccccagta caccccaca cacccaccg acgctactt 
      ccagcccac caagtccac agagagtct cctctcagt 
      gccaaagta ctccaatga ggctttgtc cgattccag 
      cgaatgtct ggagggctc ttcaccagc tccggacgc 
      ctccgaggg ccgattttt ccaaagtcc gagacagag 
      catcagtgt gagccccac tgaccccaa ctttggcta 
      ctacagtgc caggaggcc ctccaaaaa tgtagtctc 
      gaccagttc ggaggctac tagattgta attgcttca 
      atcaaaatg ggttttatt tatttattc tgcattttt 
      atgtgatat tagttgttt aatgtgttt tttgatatc 
      aatgatttt ttttgtttg ttgatgcac atgaacgaa 
      gttgacaat aaattttgt tgaatgtag agtgatttt 
      ttaagcaac tttttttca tatcatttg acatcaaac 
      aattttttc gtcaggatg attgtcaat tttataatt 
      aataaactg ttgttttat ac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY