| General information | |||
| TC002375 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NW_015452276.1: 240586..254678 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC002375 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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| Publish name | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_03364 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 3 (9 dpi)potentially confused/crosscontaminated with : adults of..., iB # (comma separated)
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
potentially confused/crosscontaminated with : adults of..., iB # (comma separated) (egglay)
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC002375 RA
MDTTKTTED VYEFKSVKE SEQTQEQKT DAQESTSEQ SEPTSTPAT QTEENSKRN FSEIADSND EVNDDESRR KKRKEEGNK DGKTPTQRN TNQAKGQSG KQGVAAQGK QTQAAAAGK TGEKKSPCS SPKPASTSD AEVEDGKSD LKVPPLKIV IPQSGSSEQ ETGTSRNGK NSSQRAHPL PYVVASSNS SDSTDKEMP PGNASPESS GSKEEKKDS NPGQSDEQR GSSHQRVLR SSHRSGPTQ GATDRGSNN SSPQLQRSH SPSPSASPA TPSTQSEVT TASGTASTQ SKAADAQAT TSNETETTA ATTTSTSTT TTAPVELHP RKRKMKPSK DVPVAAPET QETPAEAQI HPHDQPITN CYQLFLNIR KQIDKRRRG LFPVQPKPP QGFKDYLMN RCTYVLAST APTTPNISY PPTLPPQMK DLFTEQEKE RYRLRMQHV IEKEKLVLS VEQEILRVH GRAARALAN QSLPFSVCT ILRDEEVYN LITPEQEEK DRNARSRYN GRLFLSWLQ DVDDKWEKI KEHMLLRHH NEAESLHAV QKMDWEWKM KELSLCDKK STPSIDEHH VPMVHVSDD FDLLPA
- >TC002375 RB
MRRASQTHK ESTASTRVQ PRVRDDKKV TSETTKTKQ DETMDTTKT TEDVYEFKS VKESEQTQE QKTDAQEST SEQSEPTST PATQTEENS KRNFSEIAD SNDEVNDDE SRRKKRKEE GNKDGKTPT QRNTNQAKG QSGKQGVAA QGKQTQAAA AGKTGEKKS PCSSPKPAS TSDAEVEDG KSDLKVPPL KIVIPQSGS SEQETGTSR NGKNSSQRA HPLPYVVAS SNSSDSTDK EMPPGNASP ESSGSKEEK KDSNPGQSD EQRGSSHQR VLRSSHRSG PTQGATDRG SNNSSPQLQ RSHSPSPSA SPATPSTQS EVTTASGTA STQSKAADA QATTSNETE TTAATTTST STTTTAPVE LHPRKRKMK PSKDVPVAA PETQETPAE AQIHPHDQP ITNCYQLFL NIRKQIDKR RRGLFPVQP KPPQGFKDY LMNRCTYVL ASTAPTTPN ISYPPTLPP QMKDLFTEQ EKERYRLRM QHVIEKEKL VLSVEQEIL RVHGRAARA LANQSLPFS VCTILRDEE VYNLITPEQ EEKDRNARS RYNGRLFLS WLQDVDDKW EKIKEHMLL RHHNEAESL HAVQKMDWE WKMKELSLC DKKSTPSID EHHVPMVHV SDDFDLLPA
- >TC002375 RA
atggacaca accaaaaca acggaagat gtgtacgaa tttaaatcc gtaaaagaa tccgaacaa actcaggag caaaaaacc gacgctcag gagagtacg agcgaacag agtgaacct acaagtact ccagcaacg caaacggaa gaaaactcg aagagaaat ttctcagaa atcgccgat tcgaacgac gaagtgaac gacgacgaa agcaggagg aaaaaacgc aaagaagag gggaataaa gacgggaaa acgcccacg cagagaaat acgaatcag gctaaaggc caaagtggg aaacagggc gttgcggcc caagggaag caaacacaa gccgcagca gctggaaaa accggcgaa aagaaaagt ccttgttca agccccaag cccgcaagt actagtgac gcagaagta gaagatggt aaatcggat ttaaaagtc ccaccgtta aaaatagta atccctcaa agcggaagc agcgaacag gagaccggc acaagccgt aacggtaaa aacagctcc cagcgcgcc catccgttg ccgtacgtc gtcgcaagt tcgaacagc agcgactcg accgacaaa gaaatgccg cctggaaac gccagtccg gagtcctcc ggttcaaaa gaagagaaa aaggactca aatccgggc cagtccgac gaacagagg ggctccagc caccagcga gtgttgcgg agctcgcac aggtcgggc ccgacgcag ggcgccacc gacagaggc agcaataat tcgtcgccg cagctgcag aggtcgcat tcaccgtcg ccgtcggcc tcgccggcg acgccgtcc acgcagagt gaggtgacg acggcgtcg gggacggcc agcacgcag tcgaaggcg gccgatgca caagcgacg accagcaac gagacggag acgacggct gcgaccacg acgtcgacg agcacgacg acgacggcg ccggttgag ttgcatcca aggaagagg aagatgaag ccgagtaag gacgtgcca gtggcggcg cccgagacg caggagacg cccgccgag gcgcagata catccgcat gatcagccc ataactaac tgttatcag ctgtttttg aatatcagg aaacagatt gacaagaga agaagaggt ctgttcccc gttcagccc aaaccaccg caaggtttc aaagactat ttaatgaat cgttgtacg tatgtctta gctagtacc gcccccaca acccccaat atttcttac cctcccact ctcccgccc cagatgaaa gatttgttt accgaacaa gaaaaggag agatacaga ctgaggatg cagcacgta atcgaaaaa gaaaaatta gtcttatcg gtcgagcaa gaaatttta cgagtgcac ggccgagcg gctcgtgcg ctcgctaat cagtcgctt cctttttcc gtttgtacg attttacga gacgaggag gtttacaat ttgataact ccggaacag gaggaaaag gatcgaaac gcccgctcg agatacaac gggaggttg tttttgagc tggttgcaa gacgttgac gacaagtgg gagaaaatc aaagaacac atgttgttg cggcaccac aacgaggcg gaatcgttg cacgccgtt caaaaaatg gactgggag tggaaaatg aaagaactg agtttgtgt gataagaag tcaactccg tcaattgac gaacaccat gtgcctatg gtgcacgtc agtgacgac ttcgatctc ctacctgcg taa
- >TC002375 RB
atgcgtcgt gcatcgcag acccataaa gaatcgact gcatcgact cgagtacaa ccgcgtgta agggatgac aaaaaagtc acttctgag acgaccaaa accaaacaa gacgaaaca atggacaca accaaaaca acggaagat gtgtacgaa tttaaatcc gtaaaagaa tccgaacaa actcaggag caaaaaacc gacgctcag gagagtacg agcgaacag agtgaacct acaagtact ccagcaacg caaacggaa gaaaactcg aagagaaat ttctcagaa atcgccgat tcgaacgac gaagtgaac gacgacgaa agcaggagg aaaaaacgc aaagaagag gggaataaa gacgggaaa acgcccacg cagagaaat acgaatcag gctaaaggc caaagtggg aaacagggc gttgcggcc caagggaag caaacacaa gccgcagca gctggaaaa accggcgaa aagaaaagt ccttgttca agccccaag cccgcaagt actagtgac gcagaagta gaagatggt aaatcggat ttaaaagtc ccaccgtta aaaatagta atccctcaa agcggaagc agcgaacag gagaccggc acaagccgt aacggtaaa aacagctcc cagcgcgcc catccgttg ccgtacgtc gtcgcaagt tcgaacagc agcgactcg accgacaaa gaaatgccg cctggaaac gccagtccg gagtcctcc ggttcaaaa gaagagaaa aaggactca aatccgggc cagtccgac gaacagagg ggctccagc caccagcga gtgttgcgg agctcgcac aggtcgggc ccgacgcag ggcgccacc gacagaggc agcaataat tcgtcgccg cagctgcag aggtcgcat tcaccgtcg ccgtcggcc tcgccggcg acgccgtcc acgcagagt gaggtgacg acggcgtcg gggacggcc agcacgcag tcgaaggcg gccgatgca caagcgacg accagcaac gagacggag acgacggct gcgaccacg acgtcgacg agcacgacg acgacggcg ccggttgag ttgcatcca aggaagagg aagatgaag ccgagtaag gacgtgcca gtggcggcg cccgagacg caggagacg cccgccgag gcgcagata catccgcat gatcagccc ataactaac tgttatcag ctgtttttg aatatcagg aaacagatt gacaagaga agaagaggt ctgttcccc gttcagccc aaaccaccg caaggtttc aaagactat ttaatgaat cgttgtacg tatgtctta gctagtacc gcccccaca acccccaat atttcttac cctcccact ctcccgccc cagatgaaa gatttgttt accgaacaa gaaaaggag agatacaga ctgaggatg cagcacgta atcgaaaaa gaaaaatta gtcttatcg gtcgagcaa gaaatttta cgagtgcac ggccgagcg gctcgtgcg ctcgctaat cagtcgctt cctttttcc gtttgtacg attttacga gacgaggag gtttacaat ttgataact ccggaacag gaggaaaag gatcgaaac gcccgctcg agatacaac gggaggttg tttttgagc tggttgcaa gacgttgac gacaagtgg gagaaaatc aaagaacac atgttgttg cggcaccac aacgaggcg gaatcgttg cacgccgtt caaaaaatg gactgggag tggaaaatg aaagaactg agtttgtgt gataagaag tcaactccg tcaattgac gaacaccat gtgcctatg gtgcacgtc agtgacgac ttcgatctc ctacctgcg taa
- >TC002375 RA
cgcaacagc agaatccac gacgattgc acccatcgc atcgccggc ggtctccgt cgtcacctt ccgcacgca agccccatt ctcgaataa tacctaata aaagtggtc gtgcaagtg gtggtcgac aaagtccaa caaaccggc tcccaccct gagatgcgt cgtgcatcg cagacccat aaaggtacg ttgccattg ccaaaaatc cagagtcgg tcgtcgtca gggagccgc cgccgctgc cgccgccgc cgccaccac caccgcctc ctccgccag gtttagcat tgtgcgttc ctcgatccg agaatcgac tgcatcgac tcgagtaca accgcgtgt aagggatga caaaaaagt cacttctga gacgaccaa aaccaaaca agacgaaac aatggacac aaccaaaac aacggaaga tgtgtacga atttaaatc cgtaaaaga atccgaaca aactcagga gcaaaaaac cgacgctca ggagagtac gagcgaaca gagtgaacc tacaagtac tccagcaac gcaaacgga agaaaactc gaagagaaa tttctcaga aatcgccga ttcgaacga cgaagtgaa cgacgacga aagcaggag gaaaaaacg caaagaaga ggggaataa agacgggaa aacgcccac gcagagaaa tacgaatca ggctaaagg ccaaagtgg gaaacaggg cgttgcggc ccaagggaa gcaaacaca agccgcagc agctggaaa aaccggcga aaagaaaag tccttgttc aagccccaa gcccgcaag tactagtga cgcagaagt agaagatgg taaatcgga tttaaaagt cccaccgtt aaaaatagt aatccctca aagcggaag cagcgaaca ggagaccgg cacaagccg taacggtaa aaacagctc ccagcgcgc ccatccgtt gccgtacgt cgtcgcaag ttcgaacag cagcgactc gaccgacaa agaaatgcc gcctggaaa cgccagtcc ggagtcctc cggttcaaa agaagagaa aaaggactc aaatccggg ccagtccga cgaacagag gggctccag ccaccagcg agtgttgcg gagctcgca caggtcggg cccgacgca gggcgccac cgacagagg cagcaataa ttcgtcgcc gcagctgca gaggtcgca ttcaccgtc gccgtcggc ctcgccggc gacgccgtc cacgcagag tgaggtgac gacggcgtc ggggacggc cagcacgca gtcgaaggc ggccgatgc acaagcgac gaccagcaa cgagacgga gacgacggc tgcgaccac gacgtcgac gagcacgac gacgacggc gccggttga gttgcatcc aaggaagag gaagatgaa gccgagtaa ggacgtgcc agtggcggc gcccgagac gcaggagac gcccgccga ggcgcagat acatccgca tgatcagcc cataactaa ctgttatca gctgttttt gaatatcag gaaacagat tgacaagag aagaagagg tctgttccc cgttcagcc caaaccacc gcaaggttt caaagacta tttaatgaa tcgttgtac gtatgtctt agctagtac cgcccccac aacccccaa tatttctta ccctcccac tctcccgcc ccagatgaa agatttgtt taccgaaca agaaaagga gagatacag actgaggat gcagcacgt aatcgaaaa agaaaaatt agtcttatc ggtcgagca agaaatttt acgagtgca cggccgagc ggctcgtgc gctcgctaa tcagtcgct tcctttttc cgtttgtac gattttacg agacgagga ggtttacaa tttgataac tccggaaca ggaggaaaa ggatcgaaa cgcccgctc gagatacaa cgggaggtt gtttttgag ctggttgca agacgttga cgacaagtg ggagaaaat caaagaaca catgttgtt gcggcacca caacgaggc ggaatcgtt gcacgccgt tcaaaaaat ggactggga gtggaaaat gaaagaact gagtttgtg tgataagaa gtcaactcc gtcaattga cgaacacca tgtgcctat ggtgcacgt cagtgacga cttcgatct cctacctgc gtaaaatgt gattgaacc tagttttaa ggactaaac gtgaacgcg atga
- >TC002375 RB
cgcaacagc agaatccac gacgattgc acccatcgc atcgccggc ggtctccgt cgtcacctt ccgcacgca agccccatt ctcgaataa tacctaata aaagtggtc gtgcaagtg gtggtcgac aaagtccaa caaaccggc tcccaccct gagatgcgt cgtgcatcg cagacccat aaagaatcg actgcatcg actcgagta caaccgcgt gtaagggat gacaaaaaa gtcacttct gagacgacc aaaaccaaa caagacgaa acaatggac acaaccaaa acaacggaa gatgtgtac gaatttaaa tccgtaaaa gaatccgaa caaactcag gagcaaaaa accgacgct caggagagt acgagcgaa cagagtgaa cctacaagt actccagca acgcaaacg gaagaaaac tcgaagaga aatttctca gaaatcgcc gattcgaac gacgaagtg aacgacgac gaaagcagg aggaaaaaa cgcaaagaa gaggggaat aaagacggg aaaacgccc acgcagaga aatacgaat caggctaaa ggccaaagt gggaaacag ggcgttgcg gcccaaggg aagcaaaca caagccgca gcagctgga aaaaccggc gaaaagaaa agtccttgt tcaagcccc aagcccgca agtactagt gacgcagaa gtagaagat ggtaaatcg gatttaaaa gtcccaccg ttaaaaata gtaatccct caaagcgga agcagcgaa caggagacc ggcacaagc cgtaacggt aaaaacagc tcccagcgc gcccatccg ttgccgtac gtcgtcgca agttcgaac agcagcgac tcgaccgac aaagaaatg ccgcctgga aacgccagt ccggagtcc tccggttca aaagaagag aaaaaggac tcaaatccg ggccagtcc gacgaacag aggggctcc agccaccag cgagtgttg cggagctcg cacaggtcg ggcccgacg cagggcgcc accgacaga ggcagcaat aattcgtcg ccgcagctg cagaggtcg cattcaccg tcgccgtcg gcctcgccg gcgacgccg tccacgcag agtgaggtg acgacggcg tcggggacg gccagcacg cagtcgaag gcggccgat gcacaagcg acgaccagc aacgagacg gagacgacg gctgcgacc acgacgtcg acgagcacg acgacgacg gcgccggtt gagttgcat ccaaggaag aggaagatg aagccgagt aaggacgtg ccagtggcg gcgcccgag acgcaggag acgcccgcc gaggcgcag atacatccg catgatcag cccataact aactgttat cagctgttt ttgaatatc aggaaacag attgacaag agaagaaga ggtctgttc cccgttcag cccaaacca ccgcaaggt ttcaaagac tatttaatg aatcgttgt acgtatgtc ttagctagt accgccccc acaaccccc aatatttct taccctccc actctcccg ccccagatg aaagatttg tttaccgaa caagaaaag gagagatac agactgagg atgcagcac gtaatcgaa aaagaaaaa ttagtctta tcggtcgag caagaaatt ttacgagtg cacggccga gcggctcgt gcgctcgct aatcagtcg cttcctttt tccgtttgt acgatttta cgagacgag gaggtttac aatttgata actccggaa caggaggaa aaggatcga aacgcccgc tcgagatac aacgggagg ttgtttttg agctggttg caagacgtt gacgacaag tgggagaaa atcaaagaa cacatgttg ttgcggcac cacaacgag gcggaatcg ttgcacgcc gttcaaaaa atggactgg gagtggaaa atgaaagaa ctgagtttg tgtgataag aagtcaact ccgtcaatt gacgaacac catgtgcct atggtgcac gtcagtgac gacttcgat ctcctacct gcgtaaaat gtgattgaa cctagtttt aaggactaa acgtgaacg cgatga
