Status
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Gene information
General information
TC002375 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015452276.1: 240586..254678
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002375
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03364

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    potentially confused/crosscontaminated with : adults of..., iB # (comma separated)
    Adult morphology
    potentially confused/crosscontaminated with : adults of..., iB # (comma separated)
    Analysis of embryonic musculature and early development
    embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    potentially confused/crosscontaminated with : adults of..., iB # (comma separated) (egglay)
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002375 RA
      MDTTKTTED VYEFKSVKE SEQTQEQKT DAQESTSEQ 
      SEPTSTPAT QTEENSKRN FSEIADSND EVNDDESRR 
      KKRKEEGNK DGKTPTQRN TNQAKGQSG KQGVAAQGK 
      QTQAAAAGK TGEKKSPCS SPKPASTSD AEVEDGKSD 
      LKVPPLKIV IPQSGSSEQ ETGTSRNGK NSSQRAHPL 
      PYVVASSNS SDSTDKEMP PGNASPESS GSKEEKKDS 
      NPGQSDEQR GSSHQRVLR SSHRSGPTQ GATDRGSNN 
      SSPQLQRSH SPSPSASPA TPSTQSEVT TASGTASTQ 
      SKAADAQAT TSNETETTA ATTTSTSTT TTAPVELHP 
      RKRKMKPSK DVPVAAPET QETPAEAQI HPHDQPITN 
      CYQLFLNIR KQIDKRRRG LFPVQPKPP QGFKDYLMN 
      RCTYVLAST APTTPNISY PPTLPPQMK DLFTEQEKE 
      RYRLRMQHV IEKEKLVLS VEQEILRVH GRAARALAN 
      QSLPFSVCT ILRDEEVYN LITPEQEEK DRNARSRYN 
      GRLFLSWLQ DVDDKWEKI KEHMLLRHH NEAESLHAV 
      QKMDWEWKM KELSLCDKK STPSIDEHH VPMVHVSDD 
      FDLLPA
    • >TC002375 RB
      MRRASQTHK ESTASTRVQ PRVRDDKKV TSETTKTKQ 
      DETMDTTKT TEDVYEFKS VKESEQTQE QKTDAQEST 
      SEQSEPTST PATQTEENS KRNFSEIAD SNDEVNDDE 
      SRRKKRKEE GNKDGKTPT QRNTNQAKG QSGKQGVAA 
      QGKQTQAAA AGKTGEKKS PCSSPKPAS TSDAEVEDG 
      KSDLKVPPL KIVIPQSGS SEQETGTSR NGKNSSQRA 
      HPLPYVVAS SNSSDSTDK EMPPGNASP ESSGSKEEK 
      KDSNPGQSD EQRGSSHQR VLRSSHRSG PTQGATDRG 
      SNNSSPQLQ RSHSPSPSA SPATPSTQS EVTTASGTA 
      STQSKAADA QATTSNETE TTAATTTST STTTTAPVE 
      LHPRKRKMK PSKDVPVAA PETQETPAE AQIHPHDQP 
      ITNCYQLFL NIRKQIDKR RRGLFPVQP KPPQGFKDY 
      LMNRCTYVL ASTAPTTPN ISYPPTLPP QMKDLFTEQ 
      EKERYRLRM QHVIEKEKL VLSVEQEIL RVHGRAARA 
      LANQSLPFS VCTILRDEE VYNLITPEQ EEKDRNARS 
      RYNGRLFLS WLQDVDDKW EKIKEHMLL RHHNEAESL 
      HAVQKMDWE WKMKELSLC DKKSTPSID EHHVPMVHV 
      SDDFDLLPA
    CDS sequence
    • >TC002375 RA
      atggacaca accaaaaca acggaagat gtgtacgaa 
      tttaaatcc gtaaaagaa tccgaacaa actcaggag 
      caaaaaacc gacgctcag gagagtacg agcgaacag 
      agtgaacct acaagtact ccagcaacg caaacggaa 
      gaaaactcg aagagaaat ttctcagaa atcgccgat 
      tcgaacgac gaagtgaac gacgacgaa agcaggagg 
      aaaaaacgc aaagaagag gggaataaa gacgggaaa 
      acgcccacg cagagaaat acgaatcag gctaaaggc 
      caaagtggg aaacagggc gttgcggcc caagggaag 
      caaacacaa gccgcagca gctggaaaa accggcgaa 
      aagaaaagt ccttgttca agccccaag cccgcaagt 
      actagtgac gcagaagta gaagatggt aaatcggat 
      ttaaaagtc ccaccgtta aaaatagta atccctcaa 
      agcggaagc agcgaacag gagaccggc acaagccgt 
      aacggtaaa aacagctcc cagcgcgcc catccgttg 
      ccgtacgtc gtcgcaagt tcgaacagc agcgactcg 
      accgacaaa gaaatgccg cctggaaac gccagtccg 
      gagtcctcc ggttcaaaa gaagagaaa aaggactca 
      aatccgggc cagtccgac gaacagagg ggctccagc 
      caccagcga gtgttgcgg agctcgcac aggtcgggc 
      ccgacgcag ggcgccacc gacagaggc agcaataat 
      tcgtcgccg cagctgcag aggtcgcat tcaccgtcg 
      ccgtcggcc tcgccggcg acgccgtcc acgcagagt 
      gaggtgacg acggcgtcg gggacggcc agcacgcag 
      tcgaaggcg gccgatgca caagcgacg accagcaac 
      gagacggag acgacggct gcgaccacg acgtcgacg 
      agcacgacg acgacggcg ccggttgag ttgcatcca 
      aggaagagg aagatgaag ccgagtaag gacgtgcca 
      gtggcggcg cccgagacg caggagacg cccgccgag 
      gcgcagata catccgcat gatcagccc ataactaac 
      tgttatcag ctgtttttg aatatcagg aaacagatt 
      gacaagaga agaagaggt ctgttcccc gttcagccc 
      aaaccaccg caaggtttc aaagactat ttaatgaat 
      cgttgtacg tatgtctta gctagtacc gcccccaca 
      acccccaat atttcttac cctcccact ctcccgccc 
      cagatgaaa gatttgttt accgaacaa gaaaaggag 
      agatacaga ctgaggatg cagcacgta atcgaaaaa 
      gaaaaatta gtcttatcg gtcgagcaa gaaatttta 
      cgagtgcac ggccgagcg gctcgtgcg ctcgctaat 
      cagtcgctt cctttttcc gtttgtacg attttacga 
      gacgaggag gtttacaat ttgataact ccggaacag 
      gaggaaaag gatcgaaac gcccgctcg agatacaac 
      gggaggttg tttttgagc tggttgcaa gacgttgac 
      gacaagtgg gagaaaatc aaagaacac atgttgttg 
      cggcaccac aacgaggcg gaatcgttg cacgccgtt 
      caaaaaatg gactgggag tggaaaatg aaagaactg 
      agtttgtgt gataagaag tcaactccg tcaattgac 
      gaacaccat gtgcctatg gtgcacgtc agtgacgac 
      ttcgatctc ctacctgcg taa
    • >TC002375 RB
      atgcgtcgt gcatcgcag acccataaa gaatcgact 
      gcatcgact cgagtacaa ccgcgtgta agggatgac 
      aaaaaagtc acttctgag acgaccaaa accaaacaa 
      gacgaaaca atggacaca accaaaaca acggaagat 
      gtgtacgaa tttaaatcc gtaaaagaa tccgaacaa 
      actcaggag caaaaaacc gacgctcag gagagtacg 
      agcgaacag agtgaacct acaagtact ccagcaacg 
      caaacggaa gaaaactcg aagagaaat ttctcagaa 
      atcgccgat tcgaacgac gaagtgaac gacgacgaa 
      agcaggagg aaaaaacgc aaagaagag gggaataaa 
      gacgggaaa acgcccacg cagagaaat acgaatcag 
      gctaaaggc caaagtggg aaacagggc gttgcggcc 
      caagggaag caaacacaa gccgcagca gctggaaaa 
      accggcgaa aagaaaagt ccttgttca agccccaag 
      cccgcaagt actagtgac gcagaagta gaagatggt 
      aaatcggat ttaaaagtc ccaccgtta aaaatagta 
      atccctcaa agcggaagc agcgaacag gagaccggc 
      acaagccgt aacggtaaa aacagctcc cagcgcgcc 
      catccgttg ccgtacgtc gtcgcaagt tcgaacagc 
      agcgactcg accgacaaa gaaatgccg cctggaaac 
      gccagtccg gagtcctcc ggttcaaaa gaagagaaa 
      aaggactca aatccgggc cagtccgac gaacagagg 
      ggctccagc caccagcga gtgttgcgg agctcgcac 
      aggtcgggc ccgacgcag ggcgccacc gacagaggc 
      agcaataat tcgtcgccg cagctgcag aggtcgcat 
      tcaccgtcg ccgtcggcc tcgccggcg acgccgtcc 
      acgcagagt gaggtgacg acggcgtcg gggacggcc 
      agcacgcag tcgaaggcg gccgatgca caagcgacg 
      accagcaac gagacggag acgacggct gcgaccacg 
      acgtcgacg agcacgacg acgacggcg ccggttgag 
      ttgcatcca aggaagagg aagatgaag ccgagtaag 
      gacgtgcca gtggcggcg cccgagacg caggagacg 
      cccgccgag gcgcagata catccgcat gatcagccc 
      ataactaac tgttatcag ctgtttttg aatatcagg 
      aaacagatt gacaagaga agaagaggt ctgttcccc 
      gttcagccc aaaccaccg caaggtttc aaagactat 
      ttaatgaat cgttgtacg tatgtctta gctagtacc 
      gcccccaca acccccaat atttcttac cctcccact 
      ctcccgccc cagatgaaa gatttgttt accgaacaa 
      gaaaaggag agatacaga ctgaggatg cagcacgta 
      atcgaaaaa gaaaaatta gtcttatcg gtcgagcaa 
      gaaatttta cgagtgcac ggccgagcg gctcgtgcg 
      ctcgctaat cagtcgctt cctttttcc gtttgtacg 
      attttacga gacgaggag gtttacaat ttgataact 
      ccggaacag gaggaaaag gatcgaaac gcccgctcg 
      agatacaac gggaggttg tttttgagc tggttgcaa 
      gacgttgac gacaagtgg gagaaaatc aaagaacac 
      atgttgttg cggcaccac aacgaggcg gaatcgttg 
      cacgccgtt caaaaaatg gactgggag tggaaaatg 
      aaagaactg agtttgtgt gataagaag tcaactccg 
      tcaattgac gaacaccat gtgcctatg gtgcacgtc 
      agtgacgac ttcgatctc ctacctgcg taa
    mRNA sequence
    • >TC002375 RA
      cgcaacagc agaatccac gacgattgc acccatcgc 
      atcgccggc ggtctccgt cgtcacctt ccgcacgca 
      agccccatt ctcgaataa tacctaata aaagtggtc 
      gtgcaagtg gtggtcgac aaagtccaa caaaccggc 
      tcccaccct gagatgcgt cgtgcatcg cagacccat 
      aaaggtacg ttgccattg ccaaaaatc cagagtcgg 
      tcgtcgtca gggagccgc cgccgctgc cgccgccgc 
      cgccaccac caccgcctc ctccgccag gtttagcat 
      tgtgcgttc ctcgatccg agaatcgac tgcatcgac 
      tcgagtaca accgcgtgt aagggatga caaaaaagt 
      cacttctga gacgaccaa aaccaaaca agacgaaac 
      aatggacac aaccaaaac aacggaaga tgtgtacga 
      atttaaatc cgtaaaaga atccgaaca aactcagga 
      gcaaaaaac cgacgctca ggagagtac gagcgaaca 
      gagtgaacc tacaagtac tccagcaac gcaaacgga 
      agaaaactc gaagagaaa tttctcaga aatcgccga 
      ttcgaacga cgaagtgaa cgacgacga aagcaggag 
      gaaaaaacg caaagaaga ggggaataa agacgggaa 
      aacgcccac gcagagaaa tacgaatca ggctaaagg 
      ccaaagtgg gaaacaggg cgttgcggc ccaagggaa 
      gcaaacaca agccgcagc agctggaaa aaccggcga 
      aaagaaaag tccttgttc aagccccaa gcccgcaag 
      tactagtga cgcagaagt agaagatgg taaatcgga 
      tttaaaagt cccaccgtt aaaaatagt aatccctca 
      aagcggaag cagcgaaca ggagaccgg cacaagccg 
      taacggtaa aaacagctc ccagcgcgc ccatccgtt 
      gccgtacgt cgtcgcaag ttcgaacag cagcgactc 
      gaccgacaa agaaatgcc gcctggaaa cgccagtcc 
      ggagtcctc cggttcaaa agaagagaa aaaggactc 
      aaatccggg ccagtccga cgaacagag gggctccag 
      ccaccagcg agtgttgcg gagctcgca caggtcggg 
      cccgacgca gggcgccac cgacagagg cagcaataa 
      ttcgtcgcc gcagctgca gaggtcgca ttcaccgtc 
      gccgtcggc ctcgccggc gacgccgtc cacgcagag 
      tgaggtgac gacggcgtc ggggacggc cagcacgca 
      gtcgaaggc ggccgatgc acaagcgac gaccagcaa 
      cgagacgga gacgacggc tgcgaccac gacgtcgac 
      gagcacgac gacgacggc gccggttga gttgcatcc 
      aaggaagag gaagatgaa gccgagtaa ggacgtgcc 
      agtggcggc gcccgagac gcaggagac gcccgccga 
      ggcgcagat acatccgca tgatcagcc cataactaa 
      ctgttatca gctgttttt gaatatcag gaaacagat 
      tgacaagag aagaagagg tctgttccc cgttcagcc 
      caaaccacc gcaaggttt caaagacta tttaatgaa 
      tcgttgtac gtatgtctt agctagtac cgcccccac 
      aacccccaa tatttctta ccctcccac tctcccgcc 
      ccagatgaa agatttgtt taccgaaca agaaaagga 
      gagatacag actgaggat gcagcacgt aatcgaaaa 
      agaaaaatt agtcttatc ggtcgagca agaaatttt 
      acgagtgca cggccgagc ggctcgtgc gctcgctaa 
      tcagtcgct tcctttttc cgtttgtac gattttacg 
      agacgagga ggtttacaa tttgataac tccggaaca 
      ggaggaaaa ggatcgaaa cgcccgctc gagatacaa 
      cgggaggtt gtttttgag ctggttgca agacgttga 
      cgacaagtg ggagaaaat caaagaaca catgttgtt 
      gcggcacca caacgaggc ggaatcgtt gcacgccgt 
      tcaaaaaat ggactggga gtggaaaat gaaagaact 
      gagtttgtg tgataagaa gtcaactcc gtcaattga 
      cgaacacca tgtgcctat ggtgcacgt cagtgacga 
      cttcgatct cctacctgc gtaaaatgt gattgaacc 
      tagttttaa ggactaaac gtgaacgcg atga
    • >TC002375 RB
      cgcaacagc agaatccac gacgattgc acccatcgc 
      atcgccggc ggtctccgt cgtcacctt ccgcacgca 
      agccccatt ctcgaataa tacctaata aaagtggtc 
      gtgcaagtg gtggtcgac aaagtccaa caaaccggc 
      tcccaccct gagatgcgt cgtgcatcg cagacccat 
      aaagaatcg actgcatcg actcgagta caaccgcgt 
      gtaagggat gacaaaaaa gtcacttct gagacgacc 
      aaaaccaaa caagacgaa acaatggac acaaccaaa 
      acaacggaa gatgtgtac gaatttaaa tccgtaaaa 
      gaatccgaa caaactcag gagcaaaaa accgacgct 
      caggagagt acgagcgaa cagagtgaa cctacaagt 
      actccagca acgcaaacg gaagaaaac tcgaagaga 
      aatttctca gaaatcgcc gattcgaac gacgaagtg 
      aacgacgac gaaagcagg aggaaaaaa cgcaaagaa 
      gaggggaat aaagacggg aaaacgccc acgcagaga 
      aatacgaat caggctaaa ggccaaagt gggaaacag 
      ggcgttgcg gcccaaggg aagcaaaca caagccgca 
      gcagctgga aaaaccggc gaaaagaaa agtccttgt 
      tcaagcccc aagcccgca agtactagt gacgcagaa 
      gtagaagat ggtaaatcg gatttaaaa gtcccaccg 
      ttaaaaata gtaatccct caaagcgga agcagcgaa 
      caggagacc ggcacaagc cgtaacggt aaaaacagc 
      tcccagcgc gcccatccg ttgccgtac gtcgtcgca 
      agttcgaac agcagcgac tcgaccgac aaagaaatg 
      ccgcctgga aacgccagt ccggagtcc tccggttca 
      aaagaagag aaaaaggac tcaaatccg ggccagtcc 
      gacgaacag aggggctcc agccaccag cgagtgttg 
      cggagctcg cacaggtcg ggcccgacg cagggcgcc 
      accgacaga ggcagcaat aattcgtcg ccgcagctg 
      cagaggtcg cattcaccg tcgccgtcg gcctcgccg 
      gcgacgccg tccacgcag agtgaggtg acgacggcg 
      tcggggacg gccagcacg cagtcgaag gcggccgat 
      gcacaagcg acgaccagc aacgagacg gagacgacg 
      gctgcgacc acgacgtcg acgagcacg acgacgacg 
      gcgccggtt gagttgcat ccaaggaag aggaagatg 
      aagccgagt aaggacgtg ccagtggcg gcgcccgag 
      acgcaggag acgcccgcc gaggcgcag atacatccg 
      catgatcag cccataact aactgttat cagctgttt 
      ttgaatatc aggaaacag attgacaag agaagaaga 
      ggtctgttc cccgttcag cccaaacca ccgcaaggt 
      ttcaaagac tatttaatg aatcgttgt acgtatgtc 
      ttagctagt accgccccc acaaccccc aatatttct 
      taccctccc actctcccg ccccagatg aaagatttg 
      tttaccgaa caagaaaag gagagatac agactgagg 
      atgcagcac gtaatcgaa aaagaaaaa ttagtctta 
      tcggtcgag caagaaatt ttacgagtg cacggccga 
      gcggctcgt gcgctcgct aatcagtcg cttcctttt 
      tccgtttgt acgatttta cgagacgag gaggtttac 
      aatttgata actccggaa caggaggaa aaggatcga 
      aacgcccgc tcgagatac aacgggagg ttgtttttg 
      agctggttg caagacgtt gacgacaag tgggagaaa 
      atcaaagaa cacatgttg ttgcggcac cacaacgag 
      gcggaatcg ttgcacgcc gttcaaaaa atggactgg 
      gagtggaaa atgaaagaa ctgagtttg tgtgataag 
      aagtcaact ccgtcaatt gacgaacac catgtgcct 
      atggtgcac gtcagtgac gacttcgat ctcctacct 
      gcgtaaaat gtgattgaa cctagtttt aaggactaa 
      acgtgaacg cgatga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY