Status
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Gene information
General information
TC031754
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC002368
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015452276.1: 187466..191660
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031754
    iBeetle screen iB_03362

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    egg chamber mostly not present - Number of animals showing the phenotype: 4

    vitellogenic egg chamber early not present - Number of animals showing the phenotype: 4
    vitellogenic egg chamber late mostlydegenerated - Number of animals showing the phenotype: 4

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031754 RA
      MKNLRPFKK DFYVPHPAV ANRSKYEVE QYRRSKEIT 
      IDGDAPNPI QNFEEACFP DYVQHEIQK QGYDTPTAI 
      QAQGWPIAM SGKDLVGIA QTGSGKTLA YILPAIVHI 
      NNQPSIARG DGPIALVLA PTRELAQQI QQVAHDFGS 
      SSYVRNTCI FGGAPKGPQ ARDLERGVE ICIATPGRL 
      IDFLEKGTT NLQRCTYLV LDEADRMLD MGFEPQIRK 
      IIEQIRPDR QTLMWSATW PKEVRKLAQ DFLRNYVQI 
      NIGSLQLSA NHNILQIVD VCQEHEKET KLNNLLQEI 
      GNNGEPGAK IIIFVETKK KVESITRTI RRYGWPAVC 
      MHGDKSQQE RDFVLREFR NGKSSILIA TDVAARGLD 
      VEGIKYVIN YDYPNSSED YIHRIGRTG RSDTTGTSY 
      AFFTPSNFR QAKDLVSVL KEANQAINP RLSEMANRC 
      SSYGSKGGS GGGGGRWGY GGSFRGREN SGPRNHQRF 
      TNGGRSNGY SNGSY
    • >TC031754 RB
      MKNLRPFKK DFYVPHPAV ANRSKYEVE QYRRSKEIT 
      IDGDAPNPI QNFEEACFP DYVQHEIQK QGYDTPTAI 
      QAQGWPIAM SGKDLVGIA QTGSGKTLA YILPAIVHI 
      NNQPSIARG DGPIALVLA PTRELAQQI QQVAHDFGS 
      SSYVRNTCI FGGAPKGPQ ARDLERGVE ICIATPGRL 
      IDFLEKGTT NLQRCTYLV LDEADRMLD MGFEPQIRK 
      IIEQIRPDR QTLMWSATW PKEVRKLAQ DFLRNYVQI 
      NIGSLQLSA NHNILQIVD VCQEHEKET KLNNLLQEI 
      GNNGEPGAK IIIFVETKK KVESITRTI RRYGWPAVC 
      MHGDKSQQE RDFVLREFR NGKSSILIA TDVAARGLD 
      VEGIKYVIN YDYPNSSED YIHRIGRTG RSDTTGTSY 
      AFFTPSNFR QAKDLVSVL KEANQVRRT LILSQTLPF 
      WHAFAWKKT AMHEC
    CDS sequence
    • >TC031754 RA
      atgaagaac ttgcgccct ttcaaaaaa gatttctac 
      gtccctcac cccgctgtt gccaaccgg tcaaagtac 
      gaggtcgaa cagtacaga aggtccaag gaaatcacg 
      atagatggc gatgcgccc aaccctatc cagaacttc 
      gaagaggcg tgtttcccc gattatgtg caacacgag 
      atccagaaa cagggatac gacactcca acggccatc 
      caggcgcaa gggtggccc attgccatg agcgggaag 
      gatttggtg ggcattgcc cagacgggg tcagggaaa 
      accctggct tacatccta ccagcgatc gtccatatc 
      aataaccag ccatctatc gcacgtggc gatggaccc 
      atagctcta gttttggcc cccacgcga gaactcgcc 
      caacaaatc cagcaagtg gcccatgat ttcggaagt 
      tcgtcctat gttcgcaat acctgtatt tttggtggc 
      gcccccaag gggccccaa gccagggat ttggaacgt 
      ggggttgaa atttgtatt gcgactccc ggccgtttg 
      attgatttc ctggagaag ggaacaacg aatttacaa 
      agatgtacc tatttggtc ctggatgaa gctgatagg 
      atgctggat atgggtttt gagccccaa atacgcaaa 
      attattgaa cagatcaga cccgacaga cagaccttg 
      atgtggtca gccacttgg cccaaagaa gtccgaaaa 
      ttggcacaa gatttcctg agaaactac gtacaaatc 
      aatattggg tcgttgcaa ttgtcggcc aaccacaac 
      attcttcaa atcgttgat gtttgccag gaacacgaa 
      aaggaaaca aaattgaat aacttgttg caagaaatc 
      ggcaataac ggcgagcct ggagcaaaa atcattata 
      ttcgtagaa accaaaaag aaggtagag agcataacc 
      cgcacaatt agacgttac ggatggcct gccgtttgc 
      atgcatggt gacaagagc caacaggag cgcgatttc 
      gtcttgaga gagttccga aatggcaaa tcgagcatt 
      ttaattgct acagacgta gctgctcgt ggcttggat 
      gtggaagga attaaatat gtcataaac tatgactac 
      cccaactct tcagaagat tatattcac agaatagga 
      aggacaggc cgatctgac accaccggc acctcttac 
      gctttcttc actccatcc aatttccga caagccaaa 
      gatttggtt tccgttctt aaggaagca aaccaggct 
      atcaatccg agactgtct gagatggcg aatcgttgt 
      agcagttac ggcagcaaa gggggtagc ggcggtggc 
      ggtggtcgt tggggctac ggcggaagt tttcgaggg 
      cgcgaaaac agcggtcca agaaatcac cagagattc 
      actaatggc ggacgatcc aacggatat agcaacgga 
      agttattaa
    • >TC031754 RB
      atgaagaac ttgcgccct ttcaaaaaa gatttctac 
      gtccctcac cccgctgtt gccaaccgg tcaaagtac 
      gaggtcgaa cagtacaga aggtccaag gaaatcacg 
      atagatggc gatgcgccc aaccctatc cagaacttc 
      gaagaggcg tgtttcccc gattatgtg caacacgag 
      atccagaaa cagggatac gacactcca acggccatc 
      caggcgcaa gggtggccc attgccatg agcgggaag 
      gatttggtg ggcattgcc cagacgggg tcagggaaa 
      accctggct tacatccta ccagcgatc gtccatatc 
      aataaccag ccatctatc gcacgtggc gatggaccc 
      atagctcta gttttggcc cccacgcga gaactcgcc 
      caacaaatc cagcaagtg gcccatgat ttcggaagt 
      tcgtcctat gttcgcaat acctgtatt tttggtggc 
      gcccccaag gggccccaa gccagggat ttggaacgt 
      ggggttgaa atttgtatt gcgactccc ggccgtttg 
      attgatttc ctggagaag ggaacaacg aatttacaa 
      agatgtacc tatttggtc ctggatgaa gctgatagg 
      atgctggat atgggtttt gagccccaa atacgcaaa 
      attattgaa cagatcaga cccgacaga cagaccttg 
      atgtggtca gccacttgg cccaaagaa gtccgaaaa 
      ttggcacaa gatttcctg agaaactac gtacaaatc 
      aatattggg tcgttgcaa ttgtcggcc aaccacaac 
      attcttcaa atcgttgat gtttgccag gaacacgaa 
      aaggaaaca aaattgaat aacttgttg caagaaatc 
      ggcaataac ggcgagcct ggagcaaaa atcattata 
      ttcgtagaa accaaaaag aaggtagag agcataacc 
      cgcacaatt agacgttac ggatggcct gccgtttgc 
      atgcatggt gacaagagc caacaggag cgcgatttc 
      gtcttgaga gagttccga aatggcaaa tcgagcatt 
      ttaattgct acagacgta gctgctcgt ggcttggat 
      gtggaagga attaaatat gtcataaac tatgactac 
      cccaactct tcagaagat tatattcac agaatagga 
      aggacaggc cgatctgac accaccggc acctcttac 
      gctttcttc actccatcc aatttccga caagccaaa 
      gatttggtt tccgttctt aaggaagca aaccaggtg 
      aggagaacc ctaattttg agtcaaacc cttcctttt 
      tggcatgcc tttgcttgg aaaaagacg gcaatgcat 
      gaatgctga
    mRNA sequence
    • >TC031754 RA
      cgcatgtgc agaaccgtt tccactctc ccccttcca 
      gaattctcc accatcttt ctcttcaac tttggacgt 
      tgttgaggt gagtagcct ttttttgct tttccgaaa 
      atttttcta atatccacc aaaaaagtg tttttacct 
      caaaattaa gcgtttttg tcgtcactg aagcggtgg 
      aaacagagt ggactgatt ttctaatga cgtttttcg 
      ttattttgc agaaattcg caactccaa agaattgca 
      agaatcaag tttaatcac cttttgaaa acaccatcc 
      gacagtttt aatcatgtc ttacggtaa acaaaatgg 
      cggatcgta tcgaggccg tggctccga gaacggttt 
      cggaggcgg cgcaagtcg caacgggtt cgggggcgg 
      cagccgctt caagaacgg tgggggcgg gggcggtgg 
      aagccgatt cggcggccg cagcggcgg cggcggatc 
      cccgggcaa ccgcctccg aaaacccaa ttgggacat 
      gaagaactt gcgcccttt caaaaaaga tttctacgt 
      ccctcaccc cgctgttgc caaccggtc aaagtacga 
      ggtcgaaca gtacagaag gtccaagga aatcacgat 
      agatggcga tgcgcccaa ccctatcca gaacttcga 
      agaggcgtg tttccccga ttatgtgca acacgagat 
      ccagaaaca gggatacga cactccaac ggccatcca 
      ggcgcaagg gtggcccat tgccatgag cgggaagga 
      tttggtggg cattgccca gacggggtc agggaaaac 
      cctggctta catcctacc agcgatcgt ccatatcaa 
      taaccagcc atctatcgc acgtggcga tggacccat 
      agctctagt tttggcccc cacgcgaga actcgccca 
      acaaatcca gcaagtggc ccatgattt cggaagttc 
      gtcctatgt tcgcaatac ctgtatttt tggtggcgc 
      ccccaaggg gccccaagc cagggattt ggaacgtgg 
      ggttgaaat ttgtattgc gactcccgg ccgtttgat 
      tgatttcct ggagaaggg aacaacgaa tttacaaag 
      atgtaccta tttggtcct ggatgaagc tgataggat 
      gctggatat gggttttga gccccaaat acgcaaaat 
      tattgaaca gatcagacc cgacagaca gaccttgat 
      gtggtcagc cacttggcc caaagaagt ccgaaaatt 
      ggcacaaga tttcctgag aaactacgt acaaatcaa 
      tattgggtc gttgcaatt gtcggccaa ccacaacat 
      tcttcaaat cgttgatgt ttgccagga acacgaaaa 
      ggaaacaaa attgaataa cttgttgca agaaatcgg 
      caataacgg cgagcctgg agcaaaaat cattatatt 
      cgtagaaac caaaaagaa ggtagagag cataacccg 
      cacaattag acgttacgg atggcctgc cgtttgcat 
      gcatggtga caagagcca acaggagcg cgatttcgt 
      cttgagaga gttccgaaa tggcaaatc gagcatttt 
      aattgctac agacgtagc tgctcgtgg cttggatgt 
      ggaaggaat taaatatgt cataaacta tgactaccc 
      caactcttc agaagatta tattcacag aataggaag 
      gacaggccg atctgacac caccggcac ctcttacgc 
      tttcttcac tccatccaa tttccgaca agccaaaga 
      tttggtttc cgttcttaa ggaagcaaa ccaggctat 
      caatccgag actgtctga gatggcgaa tcgttgtag 
      cagttacgg cagcaaagg gggtagcgg cggtggcgg 
      tggtcgttg gggctacgg cggaagttt tcgagggcg 
      cgaaaacag cggtccaag aaatcacca gagattcac 
      taatggcgg acgatccaa cggatatag caacggaag 
      ttattaaaa ctataacag tgtggacaa gggaatgac 
      ctgatacag tatttttct tcagatgca ttccatatg 
      cgtcccaac ttagttgct attaattat tgcaaaaga 
      atttctatt tatttatgt ggtaaatat ttattgaac 
      gttcgttcg ctcgttcta aatgaattt ttttgtatt 
      atctctcaa tacatagcg attaagttc ttctcttat 
      taatgtttg taactgtga ttagattcg actttagtt 
      tatattgtc acaatataa tttttcctt atatccgac 
      agtccgtca ttagaatta atgagaaaa aataataaa 
      aggtgtttt tttac
    • >TC031754 RB
      cgcatgtgc agaaccgtt tccactctc ccccttcca 
      gaattctcc accatcttt ctcttcaac tttggacgt 
      tgttgaggt gagtagcct ttttttgct tttccgaaa 
      atttttcta atatccacc aaaaaagtg tttttacct 
      caaaattaa gcgtttttg tcgtcactg aagcggtgg 
      aaacagagt ggactgatt ttctaatga cgtttttcg 
      ttattttgc agaaattcg caactccaa agaattgca 
      agaatcaag tttaatcac cttttgaaa acaccatcc 
      gacagtttt aatcatgtc ttacggtaa acaaaatgg 
      cggatcgta tcgaggccg tggctccga gaacggttt 
      cggaggcgg cgcaagtcg caacgggtt cgggggcgg 
      cagccgctt caagaacgg tgggggcgg gggcggtgg 
      aagccgatt cggcggccg cagcggcgg cggcggatc 
      cccgggcaa ccgcctccg aaaacccaa ttgggacat 
      gaagaactt gcgcccttt caaaaaaga tttctacgt 
      ccctcaccc cgctgttgc caaccggtc aaagtacga 
      ggtcgaaca gtacagaag gtccaagga aatcacgat 
      agatggcga tgcgcccaa ccctatcca gaacttcga 
      agaggcgtg tttccccga ttatgtgca acacgagat 
      ccagaaaca gggatacga cactccaac ggccatcca 
      ggcgcaagg gtggcccat tgccatgag cgggaagga 
      tttggtggg cattgccca gacggggtc agggaaaac 
      cctggctta catcctacc agcgatcgt ccatatcaa 
      taaccagcc atctatcgc acgtggcga tggacccat 
      agctctagt tttggcccc cacgcgaga actcgccca 
      acaaatcca gcaagtggc ccatgattt cggaagttc 
      gtcctatgt tcgcaatac ctgtatttt tggtggcgc 
      ccccaaggg gccccaagc cagggattt ggaacgtgg 
      ggttgaaat ttgtattgc gactcccgg ccgtttgat 
      tgatttcct ggagaaggg aacaacgaa tttacaaag 
      atgtaccta tttggtcct ggatgaagc tgataggat 
      gctggatat gggttttga gccccaaat acgcaaaat 
      tattgaaca gatcagacc cgacagaca gaccttgat 
      gtggtcagc cacttggcc caaagaagt ccgaaaatt 
      ggcacaaga tttcctgag aaactacgt acaaatcaa 
      tattgggtc gttgcaatt gtcggccaa ccacaacat 
      tcttcaaat cgttgatgt ttgccagga acacgaaaa 
      ggaaacaaa attgaataa cttgttgca agaaatcgg 
      caataacgg cgagcctgg agcaaaaat cattatatt 
      cgtagaaac caaaaagaa ggtagagag cataacccg 
      cacaattag acgttacgg atggcctgc cgtttgcat 
      gcatggtga caagagcca acaggagcg cgatttcgt 
      cttgagaga gttccgaaa tggcaaatc gagcatttt 
      aattgctac agacgtagc tgctcgtgg cttggatgt 
      ggaaggaat taaatatgt cataaacta tgactaccc 
      caactcttc agaagatta tattcacag aataggaag 
      gacaggccg atctgacac caccggcac ctcttacgc 
      tttcttcac tccatccaa tttccgaca agccaaaga 
      tttggtttc cgttcttaa ggaagcaaa ccaggtgag 
      gagaaccct aattttgag tcaaaccct tcctttttg 
      gcatgcctt tgcttggaa aaagacggc aatgcatga 
      atgctgaag gaagggaga ttaattctt gggaaatat 
      gccttcggt gtccacaca aattctgtc tggatccca 
      tcctcaagt ttcccaaca cttctacta taattttaa 
      actgtaaac taattattt gtttgaaca ggctatcaa 
      tccgagact gtctgagat ggcgaatcg ttgtagcag 
      ttacggcag caaaggggg tagcggcgg tggcggtgg 
      tcgttgggg ctacggcgg aagttttcg agggcgcga 
      aaacagcgg tccaagaaa tcaccagag attcactaa 
      tggcggacg atccaacgg atatagcaa cggaagtta 
      ttaaaacta taacagtgt ggacaaggg aatgacctg 
      atacagtat ttttcttca gatgcattc catatgcgt 
      cccaactta gttgctatt aattattgc aaaagaatt 
      tctatttat ttatgtggt aaatattta ttgaacgtt 
      cgttcgctc gttctaaat gaatttttt tgtattatc 
      tctcaatac atagcgatt aagttcttc tcttattaa 
      tgtttgtaa ctgtgatta gattcgact ttagtttat 
      attgtcaca atataattt ttccttata tccgacagt 
      ccgtcatta gaattaatg agaaaaaat aataaaagg 
      tgttttttt ac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY