Status
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Gene information
General information
TC002365 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015452276.1: 166166..171386
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002365
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03361

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 2 number 2nd lateral oviduct: - Number of animals showing the phenotype: 2
    ovary without detectable defects - Number of animals showing the phenotype: 2
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    abdomen partially shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    posterior terminus shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002365 RA
      MKFAEHLSA HITPEWRKQ YINYEEMKA MLYMAVEEA 
      PSSESVEPE VLTRHFANF DETFFHYSD KELKKINTF 
      YSEKLAEAT RKFANLNNE LRVSLEHIR QGKRKDTDA 
      TKRHIPARK LQELKLAFS EFYLSLILL QNYQTLNHT 
      GFRKILKKH DKLLNTDVG AKWRQEHVE TSHFFTNRD 
      IDKLINDTE SMVTNELEG GDRQKAMKR LRVPPLGEQ 
      QSPWTTFKV GLFSGSFIV LLIAVVLSA IFHDGASEN 
      LKIAFRLYR GPLLIVEFL FLIGVNVYG WRSSGVNHV 
      LIFELDPRN HLSEQHLME MAAIFGVVW TLSLLSFLY 
      SSSLSIPPY VNPLALTVI MVLFVINPL KVFRHDARF 
      WFVRICGRM FGAPFFHVG FADFWLADQ LNSLANALL 
      DFQFLICFY YTHGDNWAE AGDTAECME KNFIIRPIV 
      NCIPAWIRL AQCLRRYYD TKEAFPHLV NAGKYSTTF 
      FVVIFSTLR SVYKSDYPD QSENPFLFL FIIASIVSS 
      CYAYTWDIK MDWGLFDKS AGENKFLRE EIVYSSTFF 
      YYFAIVEDF VLRFAWALS FYLTENGYV SGDLMTSIL 
      SPLEVFRRF VWNFFRLEN EHLNNCGKF RAVRDISVA 
      PIDSSDQAQ IIRMMDEAD GVANRGRNK KQAKKKEEK 
      KPLLLEESI ANFKSIPLS TMQV
    CDS sequence
    • >TC002365 RA
      atgaaattt gctgaacac ctttcagcc catatcacc 
      ccagaatgg aggaaacaa tacatcaac tacgaggaa 
      atgaaagcc atgctctac atggccgta gaagaggcc 
      ccctcgtcc gaaagcgtg gaacccgaa gtcctgact 
      cgccacttt gcaaatttc gacgaaacg ttcttccac 
      tactcggac aaagaattg aaaaaaatc aacactttc 
      tattcggaa aaactggcc gaagccact cggaaattt 
      gcaaatttg aataacgaa ctgcgagtg tctttggag 
      cacatcaga cagggtaaa aggaaagac accgatgcg 
      accaaaaga cacatcccc gccaggaaa ttgcaagaa 
      ttaaaactt gccttcagc gaattttat ttaagcttg 
      attctgttg caaaactac caaactttg aatcataca 
      gggtttcgg aagattttg aagaagcac gacaagtta 
      cttaacact gacgtgggg gcgaaatgg cggcaagag 
      cacgtcgaa acgtcccat tttttcacg aatcgagac 
      atcgacaag ctcatcaac gacaccgag agcatggtg 
      accaacgaa ctggaaggg ggcgaccgc cagaaggcc 
      atgaagcgc ctccgtgtg cctccgctg ggggagcag 
      cagtccccc tggaccacg ttcaaagtg gggctgttc 
      tcgggctcc ttcatcgtg ctcttgatt gctgtggtc 
      ctctcggcc atcttccac gacggggcg agcgagaac 
      ctcaaaatt gcattccga ttgtaccgg ggccccctc 
      ttgatcgtc gagtttttg ttcctgatt ggggtcaat 
      gtgtacggg tggaggtcg tcgggggtc aaccacgtg 
      cttattttc gagctggac ccccggaat catctctcc 
      gagcagcac ctgatggag atggcggcc atttttggg 
      gtcgtttgg acgcttagt ttgctcagt tttctctac 
      agttccagt ttaagtatc ccgccgtat gtgaatccc 
      ctggcgttg actgtcatt atggtgctg tttgtgatc 
      aatccgctc aaagtgttc agacacgat gcccgcttt 
      tggttcgtg aggatttgt ggccgcatg tttggtgcc 
      cccttcttc cacgtgggc ttcgccgat ttctggctc 
      gccgatcaa ctcaatagt ctcgccaac gcactctta 
      gatttccaa tttttgatt tgtttttac tacactcat 
      ggcgacaat tgggctgaa gcaggagat acggccgaa 
      tgtatggaa aagaatttc ataatccgt ccgattgtc 
      aactgtatc ccggcctgg atacgtctt gctcaatgc 
      cttcgccgt tattacgac accaaagaa gccttccct 
      cacttagta aacgccgga aaatactca acgacgttt 
      tttgtcgtc attttttca actctgaga tcggtttac 
      aaaagtgat tatccagac caaagcgaa aaccccttc 
      cttttccta ttcataatc gcatccatt gtgagctct 
      tgttacgcc tacacttgg gatattaaa atggattgg 
      ggccttttc gacaaatca gcgggcgaa aataaattc 
      cttcgtgag gaaatcgtc tactcttcc acgttcttc 
      tactacttt gccattgtc gaagacttt gttttacgg 
      tttgcttgg gcgctttcg ttctatttg actgaaaat 
      ggctacgtc agtggtgac ttgatgacg tccatatta 
      tcgcccctt gaggtcttc cgccgattc gtttggaac 
      tttttccga ttggaaaac gaacacttg aacaattgc 
      ggtaaattc agagccgtt cgtgatatt tccgtcgcc 
      ccaatcgac tcatctgac caagcacag ataatcagg 
      atgatggat gaggcagat ggcgttgcc aacagaggt 
      agaaacaaa aaacaggcg aagaaaaag gaagaaaag 
      aaaccgctg ttgcttgaa gaatccatc gccaacttt 
      aagtcgata ccgttgtcc acgatgcaa gtttag
    mRNA sequence
    • >TC002365 RA
      caataaatg catgaaaaa tgaaatttg ctgaacacc 
      tttcagccc atatcaccc cagaatgga ggaaacaat 
      acatcaact acgaggaaa tgaaagcca tgctctaca 
      tggccgtag aagaggccc cctcgtccg aaagcgtgg 
      aacccgaag tcctgactc gccactttg caaatttcg 
      acgaaacgt tcttccact actcggaca aagaattga 
      aaaaaatca acactttct attcggaaa aactggccg 
      aagccactc ggaaatttg caaatttga ataacgaac 
      tgcgagtgt ctttggagc acatcagac agggtaaaa 
      ggaaagaca ccgatgcga ccaaaagac acatccccg 
      ccaggaaat tgcaagaat taaaacttg ccttcagcg 
      aattttatt taagcttga ttctgttgc aaaactacc 
      aaactttga atcatacag ggtttcgga agattttga 
      agaagcacg acaagttac ttaacactg acgtggggg 
      cgaaatggc ggcaagagc acgtcgaaa cgtcccatt 
      ttttcacga atcgagaca tcgacaagc tcatcaacg 
      acaccgaga gcatggtga ccaacgaac tggaagggg 
      gcgaccgcc agaaggcca tgaagcgcc tccgtgtgc 
      ctccgctgg gggagcagc agtccccct ggaccacgt 
      tcaaagtgg ggctgttct cgggctcct tcatcgtgc 
      tcttgattg ctgtggtcc tctcggcca tcttccacg 
      acggggcga gcgagaacc tcaaaattg cattccgat 
      tgtaccggg gccccctct tgatcgtcg agtttttgt 
      tcctgattg gggtcaatg tgtacgggt ggaggtcgt 
      cgggggtca accacgtgc ttattttcg agctggacc 
      cccggaatc atctctccg agcagcacc tgatggaga 
      tggcggcca tttttgggg tcgtttgga cgcttagtt 
      tgctcagtt ttctctaca gttccagtt taagtatcc 
      cgccgtatg tgaatcccc tggcgttga ctgtcatta 
      tggtgctgt ttgtgatca atccgctca aagtgttca 
      gacacgatg cccgctttt ggttcgtga ggatttgtg 
      gccgcatgt ttggtgccc ccttcttcc acgtgggct 
      tcgccgatt tctggctcg ccgatcaac tcaatagtc 
      tcgccaacg cactcttag atttccaat ttttgattt 
      gtttttact acactcatg gcgacaatt gggctgaag 
      caggagata cggccgaat gtatggaaa agaatttca 
      taatccgtc cgattgtca actgtatcc cggcctgga 
      tacgtcttg ctcaatgcc ttcgccgtt attacgaca 
      ccaaagaag ccttccctc acttagtaa acgccggaa 
      aatactcaa cgacgtttt ttgtcgtca ttttttcaa 
      ctctgagat cggtttaca aaagtgatt atccagacc 
      aaagcgaaa accccttcc ttttcctat tcataatcg 
      catccattg tgagctctt gttacgcct acacttggg 
      atattaaaa tggattggg gccttttcg acaaatcag 
      cgggcgaaa ataaattcc ttcgtgagg aaatcgtct 
      actcttcca cgttcttct actactttg ccattgtcg 
      aagactttg ttttacggt ttgcttggg cgctttcgt 
      tctatttga ctgaaaatg gctacgtca gtggtgact 
      tgatgacgt ccatattat cgccccttg aggtcttcc 
      gccgattcg tttggaact ttttccgat tggaaaacg 
      aacacttga acaattgcg gtaaattca gagccgttc 
      gtgatattt ccgtcgccc caatcgact catctgacc 
      aagcacaga taatcagga tgatggatg aggcagatg 
      gcgttgcca acagaggta gaaacaaaa aacaggcga 
      agaaaaagg aagaaaaga aaccgctgt tgcttgaag 
      aatccatcg ccaacttta agtcgatac cgttgtcca 
      cgatgcaag tttaggaaa gatgaaatt agaagtgag 
      attaggaga cacagttgg tacttatag gtgggagca 
      gtattacgc acggatgaa ccattccct tgtgatctt 
      gtacatgtg cttattttt actattgtt aaacgaagt 
      tttacaggg attattact taacttgat gcctttata 
      caatattta gtgataatt atgtactgt acgcaaata 
      tattttaaa taaacttgg tttttatca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY