| General information | |||
| TC002347 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NW_015452276.1: 56632..88151 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC002347 | ||
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_03357 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC002347 RA
MSEAEGDCK ENSHPPSEN CKKSPLKSK ECKNSKLQQ PPKDPTRVV VVALTPDRR RETWNKKVE FLLAVVGFA VDLGNVWRF PYICYQNGG GAFLIPYTI MLVFGGLPL FYMELALGQ FHRSGCLTI WKRICPALK GIGYAICLI DIYMGMYYN TIIGWAVYY LIASFQAEL PWTSCHNYW NTKDCRPVT ALVGVNSNA SSPAKEFFE REVLEQYKS DGLNRMGPI KPALALCVF AVFVLVYFS LWKGVRSTG KAVWITALA PYVVLIILL CRGVTLPGA AEGIRYYLT PEWHKLNNS RVWIDAASQ IFFSLGPGF GTLLALSSY NKFNNNCYR DALLTSSIN CLTSFLAGF VIFSVLGYM AHVQHKSIE MVGLEGPGL VFIVYPEAI ATMTGSVFW SIIFFLMLI TLGLDSTFG GLEAMITAL CDEYPKALG RHREIFVAA LLFGIYICA LPTTTYGGV YLVNMLNVY GPGLAILFV VFVEAAGVC WLYGTDNFA RDIEKMIGH KPGIFWRMC WKYISPVFL LSIFIFSLL SHEQMLGTE YEYPEWSFK VGYALTASS IMCIPLYIV YKFAITPGS LVQRWKMIW KPEHSSDNS TLTYCGGTE V
- >TC002347 RA
atgtcggag gccgaaggc gactgcaag gagaactcg catcccccg agcgaaaac tgcaagaaa tcgccgctg aaaagcaaa gagtgcaaa aattcgaag ctgcaacag ccgcccaaa gaccccact cgtgttgtg gtggtggcc ctgactccc gaccgccgc cgagagaca tggaacaag aaagtggag tttctgttg gcggtggtg ggcttcgcc gtcgacttg ggcaacgtc tggaggttt ccctacatc tgctaccag aacggaggc ggtgcgttt ttgattccc tacaccata atgctggtg ttcggcggt ctgccgctc ttctacatg gagctcgct ctggggcag ttccaccga agcggatgt ctcacgatt tggaagcgg atttgtccg gcattaaaa ggaatcgga tacgcgatt tgtttgatc gacatttac atggggatg tactacaac accatcatt ggctgggct gtgtattat ttaattgcg tcgtttcaa gcggaactg ccgtggacg agttgtcac aactattgg aacaccaaa gactgtcgg cctgttacc gcccttgtc ggcgtcaac agtaatgcc tcaagtcct gccaaagaa tttttcgaa cgcgaggtg ctagaacaa tacaaatcg gatggcctc aacagaatg gggcctatt aagccggct ttggcgctg tgtgtcttt gcggtcttc gttcttgtt tatttctcc ctgtggaag ggcgtccgc agcaccgga aaggcggtg tggataaca gccttagcc ccctacgtc gtcctcata atcctgctc tgtcgcggg gtgaccctc ccgggggct gccgagggc atccgctac tacctcacc cccgaatgg cacaaactc aacaactcc cgcgtgtgg attgacgcc gcctcgcaa atttttttc tctctaggc cccggcttt ggcacgctc ttggccctc tccagctac aacaagttc aataataac tgctacaga gacgcgctt ttgaccagt agtatcaat tgtctgacc agttttctg gcggggttt gtgattttt tcggtgctg gggtacatg gcacacgtg cagcataaa agcatagag atggtgggg ctggagggg cccgggctg gtgtttatt gtgtatccg gaggccatt gcgacgatg actgggtcg gttttctgg tccattatt ttttttctt atgttgatt acgttgggg ttggatagc acgttcggg gggttggag gcgatgatc acggcgttg tgtgatgag taccccaag gcgttggga cggcatcgg gagattttt gtggcggcg ctcttgttc gggatctac atttgtgcc ttgccgacg actacttat gggggcgtc tatttggtg aacatgctg aacgtttac ggccccggc ttggccata ctttttgtc gtcttcgtt gaagcggca ggtgtttgt tggttgtat ggtaccgat aattttgca agagacatc gaaaagatg attggccat aagccggga attttttgg agaatgtgt tggaaatat atcagtccc gtttttctc ttgtcaatt tttattttt tcacttctc agtcacgaa caaatgttg ggcactgaa tacgagtat cccgagtgg agcttcaaa gttggctat gctttaaca gcttcgtcc atcatgtgc atccctctt tacatcgtt tacaaattt gcaataact ccaggaagc ttggtgcag aggtggaaa atgatctgg aagccggag cactcctcc gacaacagc accctcacg tactgcggc ggcaccgaa gtgtga
- >TC002347 RA
ctcattagc ccacaatgt ccacaatca atacccatc aaatcaaaa tttgcatat tgtgtaaag ttgccacaa tagtcgtgc taattgctg ttattgacg cactcgtat ccggagtct tccggcggc ggcaggcgc aggtgcccc caagaaaca gcagtctct ggttggaag caagcagtg gttcgcacg ctccagtcc agttcgcac gtcatagca ggaacagaa gcagcagca ggaagtgaa gagagcacc agcacgcat ttccgcgcg gtcgagcat gccacctga caccagacc accgccact ttaaaggga tgaactcgt ctcctcgcc cctaatcca cctccagcg caatgtcgg aggccgaag gcgactgca aggagaact cgcatcccc cgagcgaaa actgcaaga aatcgccgc tgaaaagca aagagtgca aaaattcga agctgcaac agccgccca aagacccca ctcgtgttg tggtggtgg ccctgactc ccgaccgcc gccgagaga catggaaca agaaagtgg agtttctgt tggcggtgg tgggcttcg ccgtcgact tgggcaacg tctggaggt ttccctaca tctgctacc agaacggag gcggtgcgt ttttgattc cctacacca taatgctgg tgttcggcg gtctgccgc tcttctaca tggagctcg ctctggggc agttccacc gaagcggat gtctcacga tttggaagc ggatttgtc cggcattaa aaggaatcg gatacgcga tttgtttga tcgacattt acatgggga tgtactaca acaccatca ttggctggg ctgtgtatt atttaattg cgtcgtttc aagcggaac tgccgtgga cgagttgtc acaactatt ggaacacca aagactgtc ggcctgtta ccgcccttg tcggcgtca acagtaatg cctcaagtc ctgccaaag aatttttcg aacgcgagg tgctagaac aatacaaat cggatggcc tcaacagaa tggggccta ttaagccgg ctttggcgc tgtgtgtct ttgcggtct tcgttcttg tttatttct ccctgtgga agggcgtcc gcagcaccg gaaaggcgg tgtggataa cagccttag ccccctacg tcgtcctca taatcctgc tctgtcgcg gggtgaccc tcccggggg ctgccgagg gcatccgct actacctca cccccgaat ggcacaaac tcaacaact cccgcgtgt ggattgacg ccgcctcgc aaatttttt tctctctag gccccggct ttggcacgc tcttggccc tctccagct acaacaagt tcaataata actgctaca gagacgcgc ttttgacca gtagtatca attgtctga ccagttttc tggcggggt ttgtgattt tttcggtgc tggggtaca tggcacacg tgcagcata aaagcatag agatggtgg ggctggagg ggcccgggc tggtgttta ttgtgtatc cggaggcca ttgcgacga tgactgggt cggttttct ggtccatta ttttttttc ttatgttga ttacgttgg ggttggata gcacgttcg gggggttgg aggcgatga tcacggcgt tgtgtgatg agtacccca aggcgttgg gacggcatc gggagattt ttgtggcgg cgctcttgt tcgggatct acatttgtg ccttgccga cgactactt atgggggcg tctatttgg tgaacatgc tgaacgttt acggccccg gcttggcca tactttttg tcgtcttcg ttgaagcgg caggtgttt gttggttgt atggtaccg ataattttg caagagaca tcgaaaaga tgattggcc ataagccgg gaatttttt ggagaatgt gttggaaat atatcagtc ccgtttttc tcttgtcaa tttttattt tttcacttc tcagtcacg aacaaatgt tgggcactg aatacgagt atcccgagt ggagcttca aagttggct atgctttaa cagcttcgt ccatcatgt gcatccctc tttacatcg tttacaaat ttgcaataa ctccaggaa gcttggtgc agaggtgga aaatgatct ggaagccgg agcactcct ccgacaaca gcaccctca cgtactgcg gcggcaccg aagtgtgac caacgagca ttgtcaaat catacacca tcgagtggt tagtttaag ttgttgatt tcccatttt tcgggtaaa ataccagcc atattttgc ccgaactcc cacggatta aagggttga tttaaaacg tttcgttgt tttaggtgt tttgtggcg cgagtgtaa caacagcca ttgttacac tctcgcata ttcctagtc tagagagta ttaaagcgg tgctaaatg tcgtgttaa atagtccta gacatagct gaatgtatc gctttctat gtccggggt gcagcccca cgtagtgaa ctctatcat acgtaaaat gtacaaaat cctaaactg acaaagtta aatttatcg acaataaaa cgatcgggt ttgagttcg gtggtacta aacaaaaat cgggcctac ttttgtgat acactttct gggcgaaaa gcagccaag tttcggcca gaaacgagt tgtttcttt gtgccaatt tttggttca cttgtagca ctttaggaa ttgattgtc cgatcaagc taaacaatg gaatattaa aaaaacaaa caagtc
