Status
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Gene information
General information
TC002340 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015452276.1: 202280..207394
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002340
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03356

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 6 (9 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002340 RA
      MSAVNILCL VCNGSCVSS RNSIQIFNK ETKTSSETP 
      LIDTLTKVL DKELTEDTA HSHVICKKC FKIFDEVDE 
      LEHRLDELK SELVSNYTS ALTRHKNGD NQMDESEAL 
      ENGKVTDSN KENELPRKI LDIPSSDDD TSQVMEKNQ 
      LQTIEDIEM ELVKMHGGI ENVDPVKEL TAGDIIDSG 
      AVDHSSDSD YEPPVSVES FTKSSSDIK VKMEPSGES 
      GGSDRPNIL RKKPSNPDA LKVKDEPSM HLQPMVARQ 
      DSLFSCMVC QGEETVAGD VRAITAHMK NVHDIRLYI 
      CDVCGQDFR KRNELSAHL DEHVAAEEG DFQCEVCNR 
      IFSNLRLFR IHRRMHYPQ NKAWACETC GKKYSSRNL 
      LEEHINTHT GVRPYVCQQ CGKDFASKY TFKAHEKTH 
      EVRPRPYAC NQCPKSFLS QQNLIQHER THTGIKEFS 
      CHLCGKRFG SAHNLEVHS IVHTGYRPF VCSICHKAF 
      ARKAEIRDH ERTHTGERP YQCEMCGAT FSQRSNLQS 
      HKRATHLDD KRYICVDCG KGFKRRRLL DYHMKAAHT 
      GERPFKCNV CDATFVYPE HFKKHRRIH TGEKPFLCE 
      VCGKAFNSR DNRNAHRFV HSDKKPYEC LVCGAGFMR 
      KPLLYQHMQ SQGHLNDTI VVNQPRLTT DDDQLVTVN 
      SRGEMEIVD PIALAEAVD GDGSVAEDS KLYISEHIL 
      QSPAEQEQA EGEGMETVE HIIIDGQHI RFENDDEEV 
      DTITGETLA ESETQIIET PDGPVQLVR VRIPNEHGE 
      EEEAWIKIV PE
    CDS sequence
    • >TC002340 RA
      atgtccgcg gtcaacatt ctgtgcctg gtgtgcaac 
      ggaagctgc gtctcgtcc cgcaactcg atccagatt 
      ttcaacaag gagaccaag accagctcg gagacgccg 
      cttatcgac actctgacc aaggttttg gacaaggag 
      ctgaccgaa gacaccgca cactcgcac gtcatttgc 
      aaaaagtgc ttcaaaatc ttcgatgaa gtggacgag 
      cttgagcac agactggac gaactcaaa agcgaactc 
      gttagcaac tacacgagc gccttgacc aggcacaaa 
      aacggcgac aatcaaatg gacgaaagt gaggcgctg 
      gaaaacggg aaggtgacc gactcgaac aaagaaaac 
      gaattgcct cgaaagatc ctcgatatc ccgtcgtca 
      gacgacgac acgagccaa gttatggag aagaaccaa 
      ctccaaaca atcgaggac attgagatg gagctggtg 
      aagatgcat gggggcatc gagaacgtg gacccggtc 
      aaagagtta acagccggt gatattatc gattcgggg 
      gctgtcgat cactcctcc gactcagat tacgagccc 
      ccagtcagt gtggaaagc ttcacgaaa tccagttct 
      gatataaaa gtcaaaatg gagccttct ggagaaagt 
      gggggtagt gatcgcccc aatatttta cgtaaaaag 
      ccgtcaaat ccagacgcg ttgaaagtc aaggacgaa 
      ccatcaatg catttgcaa ccaatggtg gctcggcaa 
      gactcactt tttagctgt atggtgtgt cagggtgaa 
      gagaccgtt gcaggggac gttcgtgcc atcaccgcc 
      catatgaaa aatgtgcac gacatccga ttgtacatt 
      tgcgacgtt tgtggacaa gacttccgg aagagaaac 
      gagctgtcg gcccacctc gacgagcac gtggccgcc 
      gaagagggc gatttccaa tgcgaggtc tgcaatcgc 
      attttcagt aatttgcgt ctgtttcgc atccatcga 
      cgcatgcac tacccccaa aacaaagcg tgggcatgc 
      gagacgtgt ggcaagaaa tacagtagc aggaatttg 
      ctcgaagag catataaac acccatacc ggagtccga 
      ccttacgtt tgccaacaa tgcggcaaa gatttcgcc 
      tcgaaatac accttcaaa gcacacgaa aagacccac 
      gaggtcagg ccgcgaccc tacgcctgc aaccagtgc 
      cccaagtcc ttcctgagc cagcaaaac ctgatacag 
      cacgagcgg acgcacacc gggatcaaa gagttcagc 
      tgccatttg tgtggcaag aggtttggt tcggcgcac 
      aacctcgaa gtccactcc atcgtgcac acgggctac 
      aggcccttc gtgtgctcc atctgccac aaagctttc 
      gctcgtaag gcggagatc cgcgaccac gagcgcacc 
      cacactggg gagaggccg taccagtgc gagatgtgc 
      ggtgccacc ttttcgcaa aggtccaac ttgcagtcg 
      cacaaaagg gccacgcat ctggacgac aagcgctac 
      atctgcgtg gactgcggc aaagggttc aagcggcgc 
      cggctgctg gactaccac atgaaggcg gcacatacg 
      ggcgaaagg cccttcaag tgcaacgtc tgcgacgcc 
      acgttcgtc taccccgag cacttcaag aagcacagg 
      cggatacac accggagag aagcccttc ctgtgcgag 
      gtctgcggc aaggcgttc aacagtcgc gacaaccgc 
      aacgcgcac aggtttgtg cactcggat aaaaaaccg 
      tacgagtgt ctggtctgc ggggccggg tttatgcgc 
      aagccgctc ctttaccag cacatgcag agtcagggg 
      catttgaac gacacgatc gtcgtgaat cagccgaga 
      ctgacgact gatgacgac cagctagtg acggtgaat 
      tcgcgaggg gagatggag attgtggat ccgattgcg 
      ttggctgaa gctgttgat ggtgacggg agtgtggct 
      gaagactcc aagttgtat atttctgag catattttg 
      caatcgcca gctgagcag gaacaagca gagggtgaa 
      gggatggaa acagtggag catatcata atcgatgga 
      cagcacata cggtttgag aatgatgac gaggaagtt 
      gatacgatc acaggagag acgttagct gagagcgaa 
      acgcaaatt atagaaacg ccagatggg ccagtgcag 
      ttggttcgt gtgcggata cccaacgag catggggag 
      gaggaagaa gcatggatt aaaatagtt cccgagtaa
    mRNA sequence
    • >TC002340 RA
      agcaagcac aagccgtcg ggcattttt cgtcgtgtc 
      aaattctct aacttggca gcatttgtt cgtcagccc 
      tgttttcgg cgaaaataa aaagtttcc ataaattgc 
      tccaccaat ttaaaaaag catgtccgc ggtcaacat 
      tctgtgcct ggtgtgcaa cggaagctg cgtctcgtc 
      ccgcaactc gatccagat tttcaacaa ggagaccaa 
      gaccagctc ggagacgcc gcttatcga cactctgac 
      caaggtttt ggacaagga gctgaccga agacaccgc 
      acactcgca cgtcatttg caaaaagtg cttcaaaat 
      cttcgatga agtggacga gcttgagca cagactgga 
      cgaactcaa aagcgaact cgttagcaa ctacacgag 
      cgccttgac caggcacaa aaacggcga caatcaaat 
      ggacgaaag tgaggcgct ggaaaacgg gaaggtgac 
      cgactcgaa caaagaaaa cgaattgcc tcgaaagat 
      cctcgatat cccgtcgtc agacgacga cacgagcca 
      agttatgga gaagaacca actccaaac aatcgagga 
      cattgagat ggagctggt gaagatgca tgggggcat 
      cgagaacgt ggacccggt caaagagtt aacagccgg 
      tgatattat cgattcggg ggctgtcga tcactcctc 
      cgactcaga ttacgagcc cccagtcag tgtggaaag 
      cttcacgaa atccagttc tgatataaa agtcaaaat 
      ggagccttc tggagaaag tgggggtag tgatcgccc 
      caatatttt acgtaaaaa gccgtcaaa tccagacgc 
      gttgaaagt caaggacga accatcaat gcatttgca 
      accaatggt ggctcggca agactcact ttttagctg 
      tatggtgtg tcagggtga agagaccgt tgcagggga 
      cgttcgtgc catcaccgc ccatatgaa aaatgtgca 
      cgacatccg attgtacat ttgcgacgt ttgtggaca 
      agacttccg gaagagaaa cgagctgtc ggcccacct 
      cgacgagca cgtggccgc cgaagaggg cgatttcca 
      atgcgaggt ctgcaatcg cattttcag taatttgcg 
      tctgtttcg catccatcg acgcatgca ctaccccca 
      aaacaaagc gtgggcatg cgagacgtg tggcaagaa 
      atacagtag caggaattt gctcgaaga gcatataaa 
      cacccatac cggagtccg accttacgt ttgccaaca 
      atgcggcaa agatttcgc ctcgaaata caccttcaa 
      agcacacga aaagaccca cgaggtcag gccgcgacc 
      ctacgcctg caaccagtg ccccaagtc cttcctgag 
      ccagcaaaa cctgataca gcacgagcg gacgcacac 
      cgggatcaa agagttcag ctgccattt gtgtggcaa 
      gaggtttgg ttcggcgca caacctcga agtccactc 
      catcgtgca cacgggcta caggccctt cgtgtgctc 
      catctgcca caaagcttt cgctcgtaa ggcggagat 
      ccgcgacca cgagcgcac ccacactgg ggagaggcc 
      gtaccagtg cgagatgtg cggtgccac cttttcgca 
      aaggtccaa cttgcagtc gcacaaaag ggccacgca 
      tctggacga caagcgcta catctgcgt ggactgcgg 
      caaagggtt caagcggcg ccggctgct ggactacca 
      catgaaggc ggcacatac gggcgaaag gcccttcaa 
      gtgcaacgt ctgcgacgc cacgttcgt ctaccccga 
      gcacttcaa gaagcacag gcggataca caccggaga 
      gaagccctt cctgtgcga ggtctgcgg caaggcgtt 
      caacagtcg cgacaaccg caacgcgca caggtttgt 
      gcactcgga taaaaaacc gtacgagtg tctggtctg 
      cggggccgg gtttatgcg caagccgct cctttacca 
      gcacatgca gagtcaggg gcatttgaa cgacacgat 
      cgtcgtgaa tcagccgag actgacgac tgatgacga 
      ccagctagt gacggtgaa ttcgcgagg ggagatgga 
      gattgtgga tccgattgc gttggctga agctgttga 
      tggtgacgg gagtgtggc tgaagactc caagttgta 
      tatttctga gcatatttt gcaatcgcc agctgagca 
      ggaacaagc agagggtga agggatgga aacagtgga 
      gcatatcat aatcgatgg acagcacat acggtttga 
      gaatgatga cgaggaagt tgatacgat cacaggaga 
      gacgttagc tgagagcga aacgcaaat tatagaaac 
      gccagatgg gccagtgca gttggttcg tgtgcggat 
      acccaacga gcatgggga ggaggaaga agcatggat 
      taaaatagt tcccgagta atttattta tttgactgt 
      aaaggtaaa taggttaaa acttatttt gtatttgta 
      tagagcagt taaatataa tatgaattt aaatcacac 
      actgagggg catcttgat tactttaaa ttaataacg 
      gtcttgtac aacaaatac ctttttgtc tcaatcttc 
      ggtcgcacc aagaaaaaa ctcccaatt atttaattg 
      aaaaaataa cttacaaat ccgattatc aatttgaca 
      ctttaaatc agcagagct tcatgagcg gcctcaaaa 
      aggactagt tttttatac atgcagtta aagtcgtat 
      ataatcaca actcattaa taaatattt agttttaca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY