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Gene information
General information
TC002338 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015452276.1: 215754..222674
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002338
    iBeetle screen iB_03355

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    larva partially everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

    larva everted (inside-out) - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002338 RA
      MDEQKLLKR RNKALRENN EGVLISVCT DLANLYVDK 
      GRFDFAIEE YTTVANIYK KQNKQLEYA VVSRGIGEA 
      YLGLQKFDK ALKLQKIYL DIARELKNK LEEQRALAT 
      IGHTYLTTY LDSPEPDKN NLNLANKFF MKSLVVSQS 
      LVGEASKLE IADMCARLY CNLGLVQDS LGNYDKALE 
      LFTKSINQC KAHDLYEQL QRGYMSLSS LLVKKGDSK 
      AALQQCNLA AETAKKLRN KAVLLAGIF LSKSEILLK 
      LGDLHGAKN ALHKAYKLN TPDVKERKT IENNLKVVA 
      SMCYNQDKL LVTDSTDYP TLKKLYEKM GDGACHLKL 
      YTKALDYYK KMMETAEKA EMTGPDLAS CYYSLAETY 
      KDNGQFEEA VEYFEKELV LCKNLKDSL NTLSNIADT 
      KEAANEPIS EVRAVYSRA IESCQREGN VHEEGRMLK 
      RQILYLRRQ KQHSEAFNL EQSLAKLDF HSSDSEGES 
      SDAQTESVV GAEIDLEAI TDVSDDSDV DQPTTHRRR 
      SKNFQIKRN LKGETQLHT ACIKGQLNV VRHLLDQGH 
      PVNVRDNCG WLPLHEACI HGHLDVVQL LLDKGAAIN 
      DRGGTQCEG ITPLHDAAS NGHLPIVEL LLTRGANAV 
      AKTDEGETV LNFLRKWRT GVQLNNDSQ VLYERLVEQ 
      ISLALQKSG QAVIVNDDR ETQRRSAIV EDSSNPDHR 
      SDMETEAPS TSSLRRSAI IGLEDDDSS SPDRQADDY 
      RNVMESLRR KNVKSPPKA SSSVVKRPA LLFDDDIVD 
      DWLDDDLGQ RSKKRKTAS PVKSAPSQN CQYYQPPSR 
      SPAKRPKQA KLTHFGHTR TLVEADSTS SPKAPEPAP 
      KLPRPRSFD LGTDPTLSV DVRIEKKLY RVPVLLSQV 
      ATHTIKWLA DEAAARYAK KQYIVPTLE LETKNGAVL 
      ADDDPISLL FPMGGTQAE EVEARIVKS NLPPLIDRY 
      KEACQDMGA VPRDSIGQV LQEFSVGLD LANRGFKGA 
      VLAPLVKVL NRQSNLLEL DLSGNFLTN ECFDLLAKS 
      LACLESLYK LNLNCTSLT SGHVQAIVA NLNPDVSYK 
      IIDLDFSDN WLRDESFES LALLTRRLQ LKKFNLSNN 
      KFTQNLFNH RLNKDLTLN LDQIEEFDI SDNAFDNTT 
      LITILSWLQ FANLTHLNV SRNSLSDNF LHEVLNRMW 
      TQTGPLRVC KLSNCHVTD SEVFDLLRV ATSLQHLDV 
      SYNDALTSI SLRRLLQSG IITLDIYAC GNIFQYFTE 
      FSKYLDDAS RDQNQGKTL KLSPPENSQ RETEYLIEM 
      WRELYGEKT VVELTSKFL GLSVLKV
    CDS sequence
    • >TC002338 RA
      atggacgaa caaaaactt ttgaaacgg agaaataaa 
      gccctccga gaaaacaat gaaggggtc cttatcagc 
      gtttgcacc gacttggct aatttatac gtcgacaaa 
      ggcaggttt gactttgct atagaggag tacaccact 
      gttgcaaac atttacaag aaacaaaac aagcaattg 
      gagtatgct gtagtcagt agaggcata ggtgaggcc 
      tatttgggc ttacaaaag tttgataaa gccttgaaa 
      ctccaaaaa atctactta gatattgca agggaattg 
      aaaaataag ctggaggaa caacgtgcc cttgccacc 
      attggccac acttacctc accacctac ctagattca 
      cccgaaccc gacaaaaac aacctaaac ctggccaat 
      aaatttttc atgaagagt ttagtagtg tcacagagt 
      ttagttggg gaggcgagt aaactggaa attgcagac 
      atgtgtgcg cgcctttat tgcaattta ggcttagtc 
      caggactct ctcggtaat tacgacaaa gctttggaa 
      ctttttacc aagtctata aaccagtgc aaggctcac 
      gacttgtac gagcagctg caaaggggc tacatgtcc 
      ctatcgtcc cttttggtc aaaaagggg gacagtaag 
      gcggccctt caacagtgt aatttggcc gctgagact 
      gcaaaaaaa ttacggaat aaggctgtt cttttggct 
      gggattttt ttgagcaag tctgagatt ctcttaaag 
      ttgggggat ttacacggg gccaagaac gccctccac 
      aaggcgtac aaacttaac acgccggat gtcaaggag 
      cggaaaacg atagaaaat aatttgaaa gttgtcgct 
      tcaatgtgt tacaaccag gataagctt ttggtcact 
      gatagcact gactatcct accttaaag aaattgtac 
      gaaaagatg ggcgatggc gcatgccat ttgaaactc 
      tacaccaaa gcgctcgat tattacaaa aaaatgatg 
      gagacggcg gaaaaggcc gaaatgacc ggtccggac 
      ctggcttcg tgttattac agtctggcg gagacttac 
      aaagacaac ggccagttt gaggaagct gttgagtat 
      ttcgagaaa gagttggtt ctttgcaag aatttgaag 
      gacagtttg aatactttg agcaatatt gcggataca 
      aaagaagca gcaaatgag ccgattagt gaagttaga 
      gctgtctat agccgagct atagagagt tgtcaaagg 
      gaagggaac gtacatgag gaaggccga atgttaaaa 
      cgccaaatt ctgtatttg aggcgacaa aaacaacat 
      tcggaagcg ttcaatttg gagcagagc ttggcaaaa 
      ttggatttt cacagttcg gacagtgag ggcgaaagc 
      tccgacgct cagaccgag tctgtggtg ggagcggaa 
      atcgacttg gaagctatc acagatgtc tctgatgat 
      agtgacgtt gaccaacca acgacacac cgtcgccgc 
      tccaaaaat ttccaaatc aagcgaaac ctaaaaggt 
      gagacccaa cttcacact gcttgcatt aaaggacaa 
      ttgaacgtc gtccgccac ctactcgac caaggccat 
      ccggtcaac gtcagagac aactgcggg tggctaccc 
      ctacacgaa gcctgcata cacggccac cttgacgtc 
      gtccaactc ttactagac aagggggcc gcaatcaac 
      gatcgaggg ggcacccaa tgcgaaggc ataactccc 
      ttacacgac gccgcctcc aatggacac cttccaata 
      gttgagttg ttactcact agaggtgca aacgccgtt 
      gctaagaca gacgaaggt gaaacagtt ttgaatttt 
      ttgagaaaa tggcggacc ggtgtccag ttaaataac 
      gacagtcag gtgctttat gagaggcta gtcgagcaa 
      atatcactc gctttgcaa aaatctggc caggctgtg 
      atagtaaat gacgatcga gagacgcag cgacgatcg 
      gcaattgtc gaggacagt tcaaatccc gaccaccga 
      agcgatatg gagacggag gcgccctct acgagtagt 
      ttacggcgt tcggcaatc atcgggctg gaggatgac 
      gacagttca agccccgat cgccaggcc gatgattac 
      cgaaacgtc atggagagt ctcagacgc aaaaatgtg 
      aaaagcccc ccgaaagca tcaagtagt gtggtcaaa 
      agacccgcc ctcttattc gatgatgac atcgtcgat 
      gattggttg gacgacgac cttgggcaa aggtcaaaa 
      aaacgcaaa acggcctcg ccggtaaag tcggcccct 
      tcccaaaac tgtcagtat taccaacct ccatcgcgc 
      agtcctgcc aaacgacca aaacaagcc aaactgaca 
      cacttcggc cacactagg accctggtc gaggccgac 
      tcgacctcg tcgccgaaa gcacccgaa cccgctccg 
      aaactgccc cgacctcgc tcgttcgac ctcggcacc 
      gacccgacc ttatccgta gacgtccga atcgagaaa 
      aaactatac cgagtcccg gttctccta tcgcaggtg 
      gccacccac acaatcaag tggctagcg gacgaggcg 
      gccgcccga tacgccaaa aaacaatac atagtgcca 
      acattagaa ctcgagact aaaaacggg gcggttttg 
      gccgatgac gacccgatt agtcttttg ttcccaatg 
      gggggaacc caggccgaa gaggtcgag gcccgcatt 
      gttaaatca aatcttccg cctcttata gaccgctac 
      aaggaggcg tgtcaggac atgggggcg gtgccacgt 
      gactcgatt ggtcaagtc ctgcaggag ttttcggtg 
      gggttggat ttggccaat cgggggttc aagggggcg 
      gtgctagcc cccctggtc aaggtgtta aaccggcaa 
      agcaaccta ctcgagttg gacttatcc ggcaatttt 
      ttaacaaac gagtgtttc gacttgttg gccaaaagt 
      ttggcctgt ttggagagc ttgtacaag ttgaatttg 
      aactgcaca agtctcact tcggggcat gtgcaagcc 
      atcgtagcc aacttaaac ccggacgtt tcgtacaaa 
      ataatagat ctcgacttt agcgacaat tggttacgt 
      gatgaaagc ttcgaaagc ttggccttg ttaacacgc 
      cgattacaa ctaaaaaag ttcaatttg tcgaataat 
      aaattcacg caaaaccta ttcaatcac cgcctaaac 
      aaagacttg accctaaat ttggaccaa atcgaagaa 
      ttcgacatc agtgataac gccttcgat aataccacc 
      cttatcact atattatca tggctccag ttcgccaac 
      ttaacacac ttgaacgtg tcacgaaac tctcttagt 
      gacaacttt ttacacgaa gtgctcaac cgaatgtgg 
      acccaaacg ggtccgtta cgcgtttgc aagctgtca 
      aattgtcat gtgaccgat tcggaggtg tttgatttg 
      ttgcgcgtt gccacgtcg cttcaacac ttggatgtt 
      tcctacaat gatgctttg acgagtatt tcgttgagg 
      agattgcta cagagtggc atcatcacg ttggatatt 
      tacgcttgt gggaacatt tttcagtat tttaccgaa 
      ttttctaag tatttggat gacgcgtca cgcgatcaa 
      aatcaaggt aagacgctt aagttaagc ccgccggaa 
      aattcgcaa cgcgagact gagtatttg atcgagatg 
      tggagggag ttgtatggg gagaagaca gtcgtggaa 
      ttgacaagt aagtttttg gggttgtct gtattgaaa 
      gtgtaa
    mRNA sequence
    • >TC002338 RA
      aggcttaat tcttgtttt tattcaagc cgtaaaggt 
      tctgtgaaa atgggactt aaaaaaaac aagttttat 
      gcatatttc tgtttccaa gccaaggtg cgtagcaat 
      gttgtgctg gtcgactat aattttcaa acgataacc 
      ttttgcgtt gctatcatt accggcaaa ctttgacag 
      tgacagctg ttttggagg gaaaggtat ttgtcaaaa 
      aggcaaaca tgcataaaa atgatcatc atgtgttta 
      tgtacttga gtcactatt aataacaga aaaaatgga 
      cgaacaaaa acttttgaa acggagaaa taaagccct 
      ccgagaaaa caatgaagg ggtccttat cagcgtttg 
      caccgactt ggctaattt atacgtcga caaaggcag 
      gtttgactt tgctataga ggagtacac cactgttgc 
      aaacattta caagaaaca aaacaagca attggagta 
      tgctgtagt cagtagagg cataggtga ggcctattt 
      gggcttaca aaagtttga taaagcctt gaaactcca 
      aaaaatcta cttagatat tgcaaggga attgaaaaa 
      taagctgga ggaacaacg tgcccttgc caccattgg 
      ccacactta cctcaccac ctacctaga ttcacccga 
      acccgacaa aaacaacct aaacctggc caataaatt 
      tttcatgaa gagtttagt agtgtcaca gagtttagt 
      tggggaggc gagtaaact ggaaattgc agacatgtg 
      tgcgcgcct ttattgcaa tttaggctt agtccagga 
      ctctctcgg taattacga caaagcttt ggaactttt 
      taccaagtc tataaacca gtgcaaggc tcacgactt 
      gtacgagca gctgcaaag gggctacat gtccctatc 
      gtccctttt ggtcaaaaa gggggacag taaggcggc 
      ccttcaaca gtgtaattt ggccgctga gactgcaaa 
      aaaattacg gaataaggc tgttctttt ggctgggat 
      ttttttgag caagtctga gattctctt aaagttggg 
      ggatttaca cggggccaa gaacgccct ccacaaggc 
      gtacaaact taacacgcc ggatgtcaa ggagcggaa 
      aacgataga aaataattt gaaagttgt cgcttcaat 
      gtgttacaa ccaggataa gcttttggt cactgatag 
      cactgacta tcctacctt aaagaaatt gtacgaaaa 
      gatgggcga tggcgcatg ccatttgaa actctacac 
      caaagcgct cgattatta caaaaaaat gatggagac 
      ggcggaaaa ggccgaaat gaccggtcc ggacctggc 
      ttcgtgtta ttacagtct ggcggagac ttacaaaga 
      caacggcca gtttgagga agctgttga gtatttcga 
      gaaagagtt ggttctttg caagaattt gaaggacag 
      tttgaatac tttgagcaa tattgcgga tacaaaaga 
      agcagcaaa tgagccgat tagtgaagt tagagctgt 
      ctatagccg agctataga gagttgtca aagggaagg 
      gaacgtaca tgaggaagg ccgaatgtt aaaacgcca 
      aattctgta tttgaggcg acaaaaaca acattcgga 
      agcgttcaa tttggagca gagcttggc aaaattgga 
      ttttcacag ttcggacag tgagggcga aagctccga 
      cgctcagac cgagtctgt ggtgggagc ggaaatcga 
      cttggaagc tatcacaga tgtctctga tgatagtga 
      cgttgacca accaacgac acaccgtcg ccgctccaa 
      aaatttcca aatcaagcg aaacctaaa aggtgagac 
      ccaacttca cactgcttg cattaaagg acaattgaa 
      cgtcgtccg ccacctact cgaccaagg ccatccggt 
      caacgtcag agacaactg cgggtggct acccctaca 
      cgaagcctg catacacgg ccaccttga cgtcgtcca 
      actcttact agacaaggg ggccgcaat caacgatcg 
      agggggcac ccaatgcga aggcataac tcccttaca 
      cgacgccgc ctccaatgg acaccttcc aatagttga 
      gttgttact cactagagg tgcaaacgc cgttgctaa 
      gacagacga aggtgaaac agttttgaa ttttttgag 
      aaaatggcg gaccggtgt ccagttaaa taacgacag 
      tcaggtgct ttatgagag gctagtcga gcaaatatc 
      actcgcttt gcaaaaatc tggccaggc tgtgatagt 
      aaatgacga tcgagagac gcagcgacg atcggcaat 
      tgtcgagga cagttcaaa tcccgacca ccgaagcga 
      tatggagac ggaggcgcc ctctacgag tagtttacg 
      gcgttcggc aatcatcgg gctggagga tgacgacag 
      ttcaagccc cgatcgcca ggccgatga ttaccgaaa 
      cgtcatgga gagtctcag acgcaaaaa tgtgaaaag 
      ccccccgaa agcatcaag tagtgtggt caaaagacc 
      cgccctctt attcgatga tgacatcgt cgatgattg 
      gttggacga cgaccttgg gcaaaggtc aaaaaaacg 
      caaaacggc ctcgccggt aaagtcggc cccttccca 
      aaactgtca gtattacca acctccatc gcgcagtcc 
      tgccaaacg accaaaaca agccaaact gacacactt 
      cggccacac taggaccct ggtcgaggc cgactcgac 
      ctcgtcgcc gaaagcacc cgaacccgc tccgaaact 
      gccccgacc tcgctcgtt cgacctcgg caccgaccc 
      gaccttatc cgtagacgt ccgaatcga gaaaaaact 
      ataccgagt cccggttct cctatcgca ggtggccac 
      ccacacaat caagtggct agcggacga ggcggccgc 
      ccgatacgc caaaaaaca atacatagt gccaacatt 
      agaactcga gactaaaaa cggggcggt tttggccga 
      tgacgaccc gattagtct tttgttccc aatgggggg 
      aacccaggc cgaagaggt cgaggcccg cattgttaa 
      atcaaatct tccgcctct tatagaccg ctacaagga 
      ggcgtgtca ggacatggg ggcggtgcc acgtgactc 
      gattggtca agtcctgca ggagttttc ggtggggtt 
      ggatttggc caatcgggg gttcaaggg ggcggtgct 
      agcccccct ggtcaaggt gttaaaccg gcaaagcaa 
      cctactcga gttggactt atccggcaa ttttttaac 
      aaacgagtg tttcgactt gttggccaa aagtttggc 
      ctgtttgga gagcttgta caagttgaa tttgaactg 
      cacaagtct cacttcggg gcatgtgca agccatcgt 
      agccaactt aaacccgga cgtttcgta caaaataat 
      agatctcga ctttagcga caattggtt acgtgatga 
      aagcttcga aagcttggc cttgttaac acgccgatt 
      acaactaaa aaagttcaa tttgtcgaa taataaatt 
      cacgcaaaa cctattcaa tcaccgcct aaacaaaga 
      cttgaccct aaatttgga ccaaatcga agaattcga 
      catcagtga taacgcctt cgataatac cacccttat 
      cactatatt atcatggct ccagttcgc caacttaac 
      acacttgaa cgtgtcacg aaactctct tagtgacaa 
      ctttttaca cgaagtgct caaccgaat gtggaccca 
      aacgggtcc gttacgcgt ttgcaagct gtcaaattg 
      tcatgtgac cgattcgga ggtgtttga tttgttgcg 
      cgttgccac gtcgcttca acacttgga tgtttccta 
      caatgatgc tttgacgag tatttcgtt gaggagatt 
      gctacagag tggcatcat cacgttgga tatttacgc 
      ttgtgggaa catttttca gtattttac cgaattttc 
      taagtattt ggatgacgc gtcacgcga tcaaaatca 
      aggtaagac gcttaagtt aagcccgcc ggaaaattc 
      gcaacgcga gactgagta tttgatcga gatgtggag 
      ggagttgta tggggagaa gacagtcgt ggaattgac 
      aagtaagtt tttggggtt gtctgtatt gaaagtgta 
      attttttta taaaataaa atttatttt tatatttac 
      aatacaacc gtaattttt ttaacattc tgacaacaa 
      gaatctaat cagtcccga attttcctt ttgtttaga 
      aggtgatta cggcggtgt atacgtttt atttaatta 
      caaaagttg tcttatatg gccacgtaa aataataaa 
      attataaag acgactata aagtacaaa gacagaaat 
      aaagaagag acttgcctt taaagccta ttttgtgca 
      gttatgaaa ggcaattgc tacgtaatc gtatcacaa 
      tataatgac ttacttact aaactaaga gaacctaaa 
      taagtggtc cttatggca ataaatctc aatatctgt 
      ttcaaaatt ctgagtcac agctaaaca aaaaaagtt 
      gcataaaat ggttaaaat cacaagcgt catcaactt 
      gctgaattt cgttaaaaa taatttttg catatttga 
      ggcctgatt tctagaacg attaaagtt gagttaatt 
      tttatctct cacctgatt ggcgttgtt atattttaa 
      ctgaggttt aaatttatc tttccgtta aatatgttt 
      ccacaaact gggcttaag caatttttc aggtaatta 
      tttcagttt ttcaaaaaa aaatgcaaa aacttgctg 
      taaaatcac aaaattgga tcgaacttg acgttattt 
      gaacttgtt ttgctcgat ttggtttgt ttttaaacc 
      aagaactta tttatttgg caggatatt tttctcaag 
      atacactta caaattaag ataaataga ttttgctta 
      tttcactct ttttttttt tgagtgaaa tttcatcaa 
      aaataatcc gaaaaccag aag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY