Status
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Gene information
General information
TC002338 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015452276.1: 215754..222674
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC002338
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03355

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     larva partially everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

    larva everted (inside-out) - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002338 RA
      MDEQKLLKR RNKALRENN EGVLISVCT DLANLYVDK 
GRFDFAIEE YTTVANIYK KQNKQLEYA VVSRGIGEA 
YLGLQKFDK ALKLQKIYL DIARELKNK LEEQRALAT 
IGHTYLTTY LDSPEPDKN NLNLANKFF MKSLVVSQS 
LVGEASKLE IADMCARLY CNLGLVQDS LGNYDKALE 
LFTKSINQC KAHDLYEQL QRGYMSLSS LLVKKGDSK 
AALQQCNLA AETAKKLRN KAVLLAGIF LSKSEILLK 
LGDLHGAKN ALHKAYKLN TPDVKERKT IENNLKVVA 
SMCYNQDKL LVTDSTDYP TLKKLYEKM GDGACHLKL 
YTKALDYYK KMMETAEKA EMTGPDLAS CYYSLAETY 
KDNGQFEEA VEYFEKELV LCKNLKDSL NTLSNIADT 
KEAANEPIS EVRAVYSRA IESCQREGN VHEEGRMLK 
RQILYLRRQ KQHSEAFNL EQSLAKLDF HSSDSEGES 
SDAQTESVV GAEIDLEAI TDVSDDSDV DQPTTHRRR 
SKNFQIKRN LKGETQLHT ACIKGQLNV VRHLLDQGH 
PVNVRDNCG WLPLHEACI HGHLDVVQL LLDKGAAIN 
DRGGTQCEG ITPLHDAAS NGHLPIVEL LLTRGANAV 
AKTDEGETV LNFLRKWRT GVQLNNDSQ VLYERLVEQ 
ISLALQKSG QAVIVNDDR ETQRRSAIV EDSSNPDHR 
SDMETEAPS TSSLRRSAI IGLEDDDSS SPDRQADDY 
RNVMESLRR KNVKSPPKA SSSVVKRPA LLFDDDIVD 
DWLDDDLGQ RSKKRKTAS PVKSAPSQN CQYYQPPSR 
SPAKRPKQA KLTHFGHTR TLVEADSTS SPKAPEPAP 
KLPRPRSFD LGTDPTLSV DVRIEKKLY RVPVLLSQV 
ATHTIKWLA DEAAARYAK KQYIVPTLE LETKNGAVL 
ADDDPISLL FPMGGTQAE EVEARIVKS NLPPLIDRY 
KEACQDMGA VPRDSIGQV LQEFSVGLD LANRGFKGA 
VLAPLVKVL NRQSNLLEL DLSGNFLTN ECFDLLAKS 
LACLESLYK LNLNCTSLT SGHVQAIVA NLNPDVSYK 
IIDLDFSDN WLRDESFES LALLTRRLQ LKKFNLSNN 
KFTQNLFNH RLNKDLTLN LDQIEEFDI SDNAFDNTT 
LITILSWLQ FANLTHLNV SRNSLSDNF LHEVLNRMW 
TQTGPLRVC KLSNCHVTD SEVFDLLRV ATSLQHLDV 
SYNDALTSI SLRRLLQSG IITLDIYAC GNIFQYFTE 
FSKYLDDAS RDQNQGKTL KLSPPENSQ RETEYLIEM 
WRELYGEKT VVELTSKFL GLSVLKV
    CDS sequence
    • >TC002338 RA
      atggacgaa caaaaactt ttgaaacgg agaaataaa 
gccctccga gaaaacaat gaaggggtc cttatcagc 
gtttgcacc gacttggct aatttatac gtcgacaaa 
ggcaggttt gactttgct atagaggag tacaccact 
gttgcaaac atttacaag aaacaaaac aagcaattg 
gagtatgct gtagtcagt agaggcata ggtgaggcc 
tatttgggc ttacaaaag tttgataaa gccttgaaa 
ctccaaaaa atctactta gatattgca agggaattg 
aaaaataag ctggaggaa caacgtgcc cttgccacc 
attggccac acttacctc accacctac ctagattca 
cccgaaccc gacaaaaac aacctaaac ctggccaat 
aaatttttc atgaagagt ttagtagtg tcacagagt 
ttagttggg gaggcgagt aaactggaa attgcagac 
atgtgtgcg cgcctttat tgcaattta ggcttagtc 
caggactct ctcggtaat tacgacaaa gctttggaa 
ctttttacc aagtctata aaccagtgc aaggctcac 
gacttgtac gagcagctg caaaggggc tacatgtcc 
ctatcgtcc cttttggtc aaaaagggg gacagtaag 
gcggccctt caacagtgt aatttggcc gctgagact 
gcaaaaaaa ttacggaat aaggctgtt cttttggct 
gggattttt ttgagcaag tctgagatt ctcttaaag 
ttgggggat ttacacggg gccaagaac gccctccac 
aaggcgtac aaacttaac acgccggat gtcaaggag 
cggaaaacg atagaaaat aatttgaaa gttgtcgct 
tcaatgtgt tacaaccag gataagctt ttggtcact 
gatagcact gactatcct accttaaag aaattgtac 
gaaaagatg ggcgatggc gcatgccat ttgaaactc 
tacaccaaa gcgctcgat tattacaaa aaaatgatg 
gagacggcg gaaaaggcc gaaatgacc ggtccggac 
ctggcttcg tgttattac agtctggcg gagacttac 
aaagacaac ggccagttt gaggaagct gttgagtat 
ttcgagaaa gagttggtt ctttgcaag aatttgaag 
gacagtttg aatactttg agcaatatt gcggataca 
aaagaagca gcaaatgag ccgattagt gaagttaga 
gctgtctat agccgagct atagagagt tgtcaaagg 
gaagggaac gtacatgag gaaggccga atgttaaaa 
cgccaaatt ctgtatttg aggcgacaa aaacaacat 
tcggaagcg ttcaatttg gagcagagc ttggcaaaa 
ttggatttt cacagttcg gacagtgag ggcgaaagc 
tccgacgct cagaccgag tctgtggtg ggagcggaa 
atcgacttg gaagctatc acagatgtc tctgatgat 
agtgacgtt gaccaacca acgacacac cgtcgccgc 
tccaaaaat ttccaaatc aagcgaaac ctaaaaggt 
gagacccaa cttcacact gcttgcatt aaaggacaa 
ttgaacgtc gtccgccac ctactcgac caaggccat 
ccggtcaac gtcagagac aactgcggg tggctaccc 
ctacacgaa gcctgcata cacggccac cttgacgtc 
gtccaactc ttactagac aagggggcc gcaatcaac 
gatcgaggg ggcacccaa tgcgaaggc ataactccc 
ttacacgac gccgcctcc aatggacac cttccaata 
gttgagttg ttactcact agaggtgca aacgccgtt 
gctaagaca gacgaaggt gaaacagtt ttgaatttt 
ttgagaaaa tggcggacc ggtgtccag ttaaataac 
gacagtcag gtgctttat gagaggcta gtcgagcaa 
atatcactc gctttgcaa aaatctggc caggctgtg 
atagtaaat gacgatcga gagacgcag cgacgatcg 
gcaattgtc gaggacagt tcaaatccc gaccaccga 
agcgatatg gagacggag gcgccctct acgagtagt 
ttacggcgt tcggcaatc atcgggctg gaggatgac 
gacagttca agccccgat cgccaggcc gatgattac 
cgaaacgtc atggagagt ctcagacgc aaaaatgtg 
aaaagcccc ccgaaagca tcaagtagt gtggtcaaa 
agacccgcc ctcttattc gatgatgac atcgtcgat 
gattggttg gacgacgac cttgggcaa aggtcaaaa 
aaacgcaaa acggcctcg ccggtaaag tcggcccct 
tcccaaaac tgtcagtat taccaacct ccatcgcgc 
agtcctgcc aaacgacca aaacaagcc aaactgaca 
cacttcggc cacactagg accctggtc gaggccgac 
tcgacctcg tcgccgaaa gcacccgaa cccgctccg 
aaactgccc cgacctcgc tcgttcgac ctcggcacc 
gacccgacc ttatccgta gacgtccga atcgagaaa 
aaactatac cgagtcccg gttctccta tcgcaggtg 
gccacccac acaatcaag tggctagcg gacgaggcg 
gccgcccga tacgccaaa aaacaatac atagtgcca 
acattagaa ctcgagact aaaaacggg gcggttttg 
gccgatgac gacccgatt agtcttttg ttcccaatg 
gggggaacc caggccgaa gaggtcgag gcccgcatt 
gttaaatca aatcttccg cctcttata gaccgctac 
aaggaggcg tgtcaggac atgggggcg gtgccacgt 
gactcgatt ggtcaagtc ctgcaggag ttttcggtg 
gggttggat ttggccaat cgggggttc aagggggcg 
gtgctagcc cccctggtc aaggtgtta aaccggcaa 
agcaaccta ctcgagttg gacttatcc ggcaatttt 
ttaacaaac gagtgtttc gacttgttg gccaaaagt 
ttggcctgt ttggagagc ttgtacaag ttgaatttg 
aactgcaca agtctcact tcggggcat gtgcaagcc 
atcgtagcc aacttaaac ccggacgtt tcgtacaaa 
ataatagat ctcgacttt agcgacaat tggttacgt 
gatgaaagc ttcgaaagc ttggccttg ttaacacgc 
cgattacaa ctaaaaaag ttcaatttg tcgaataat 
aaattcacg caaaaccta ttcaatcac cgcctaaac 
aaagacttg accctaaat ttggaccaa atcgaagaa 
ttcgacatc agtgataac gccttcgat aataccacc 
cttatcact atattatca tggctccag ttcgccaac 
ttaacacac ttgaacgtg tcacgaaac tctcttagt 
gacaacttt ttacacgaa gtgctcaac cgaatgtgg 
acccaaacg ggtccgtta cgcgtttgc aagctgtca 
aattgtcat gtgaccgat tcggaggtg tttgatttg 
ttgcgcgtt gccacgtcg cttcaacac ttggatgtt 
tcctacaat gatgctttg acgagtatt tcgttgagg 
agattgcta cagagtggc atcatcacg ttggatatt 
tacgcttgt gggaacatt tttcagtat tttaccgaa 
ttttctaag tatttggat gacgcgtca cgcgatcaa 
aatcaaggt aagacgctt aagttaagc ccgccggaa 
aattcgcaa cgcgagact gagtatttg atcgagatg 
tggagggag ttgtatggg gagaagaca gtcgtggaa 
ttgacaagt aagtttttg gggttgtct gtattgaaa 
gtgtaa
    mRNA sequence
    • >TC002338 RA
      aggcttaat tcttgtttt tattcaagc cgtaaaggt 
tctgtgaaa atgggactt aaaaaaaac aagttttat 
gcatatttc tgtttccaa gccaaggtg cgtagcaat 
gttgtgctg gtcgactat aattttcaa acgataacc 
ttttgcgtt gctatcatt accggcaaa ctttgacag 
tgacagctg ttttggagg gaaaggtat ttgtcaaaa 
aggcaaaca tgcataaaa atgatcatc atgtgttta 
tgtacttga gtcactatt aataacaga aaaaatgga 
cgaacaaaa acttttgaa acggagaaa taaagccct 
ccgagaaaa caatgaagg ggtccttat cagcgtttg 
caccgactt ggctaattt atacgtcga caaaggcag 
gtttgactt tgctataga ggagtacac cactgttgc 
aaacattta caagaaaca aaacaagca attggagta 
tgctgtagt cagtagagg cataggtga ggcctattt 
gggcttaca aaagtttga taaagcctt gaaactcca 
aaaaatcta cttagatat tgcaaggga attgaaaaa 
taagctgga ggaacaacg tgcccttgc caccattgg 
ccacactta cctcaccac ctacctaga ttcacccga 
acccgacaa aaacaacct aaacctggc caataaatt 
tttcatgaa gagtttagt agtgtcaca gagtttagt 
tggggaggc gagtaaact ggaaattgc agacatgtg 
tgcgcgcct ttattgcaa tttaggctt agtccagga 
ctctctcgg taattacga caaagcttt ggaactttt 
taccaagtc tataaacca gtgcaaggc tcacgactt 
gtacgagca gctgcaaag gggctacat gtccctatc 
gtccctttt ggtcaaaaa gggggacag taaggcggc 
ccttcaaca gtgtaattt ggccgctga gactgcaaa 
aaaattacg gaataaggc tgttctttt ggctgggat 
ttttttgag caagtctga gattctctt aaagttggg 
ggatttaca cggggccaa gaacgccct ccacaaggc 
gtacaaact taacacgcc ggatgtcaa ggagcggaa 
aacgataga aaataattt gaaagttgt cgcttcaat 
gtgttacaa ccaggataa gcttttggt cactgatag 
cactgacta tcctacctt aaagaaatt gtacgaaaa 
gatgggcga tggcgcatg ccatttgaa actctacac 
caaagcgct cgattatta caaaaaaat gatggagac 
ggcggaaaa ggccgaaat gaccggtcc ggacctggc 
ttcgtgtta ttacagtct ggcggagac ttacaaaga 
caacggcca gtttgagga agctgttga gtatttcga 
gaaagagtt ggttctttg caagaattt gaaggacag 
tttgaatac tttgagcaa tattgcgga tacaaaaga 
agcagcaaa tgagccgat tagtgaagt tagagctgt 
ctatagccg agctataga gagttgtca aagggaagg 
gaacgtaca tgaggaagg ccgaatgtt aaaacgcca 
aattctgta tttgaggcg acaaaaaca acattcgga 
agcgttcaa tttggagca gagcttggc aaaattgga 
ttttcacag ttcggacag tgagggcga aagctccga 
cgctcagac cgagtctgt ggtgggagc ggaaatcga 
cttggaagc tatcacaga tgtctctga tgatagtga 
cgttgacca accaacgac acaccgtcg ccgctccaa 
aaatttcca aatcaagcg aaacctaaa aggtgagac 
ccaacttca cactgcttg cattaaagg acaattgaa 
cgtcgtccg ccacctact cgaccaagg ccatccggt 
caacgtcag agacaactg cgggtggct acccctaca 
cgaagcctg catacacgg ccaccttga cgtcgtcca 
actcttact agacaaggg ggccgcaat caacgatcg 
agggggcac ccaatgcga aggcataac tcccttaca 
cgacgccgc ctccaatgg acaccttcc aatagttga 
gttgttact cactagagg tgcaaacgc cgttgctaa 
gacagacga aggtgaaac agttttgaa ttttttgag 
aaaatggcg gaccggtgt ccagttaaa taacgacag 
tcaggtgct ttatgagag gctagtcga gcaaatatc 
actcgcttt gcaaaaatc tggccaggc tgtgatagt 
aaatgacga tcgagagac gcagcgacg atcggcaat 
tgtcgagga cagttcaaa tcccgacca ccgaagcga 
tatggagac ggaggcgcc ctctacgag tagtttacg 
gcgttcggc aatcatcgg gctggagga tgacgacag 
ttcaagccc cgatcgcca ggccgatga ttaccgaaa 
cgtcatgga gagtctcag acgcaaaaa tgtgaaaag 
ccccccgaa agcatcaag tagtgtggt caaaagacc 
cgccctctt attcgatga tgacatcgt cgatgattg 
gttggacga cgaccttgg gcaaaggtc aaaaaaacg 
caaaacggc ctcgccggt aaagtcggc cccttccca 
aaactgtca gtattacca acctccatc gcgcagtcc 
tgccaaacg accaaaaca agccaaact gacacactt 
cggccacac taggaccct ggtcgaggc cgactcgac 
ctcgtcgcc gaaagcacc cgaacccgc tccgaaact 
gccccgacc tcgctcgtt cgacctcgg caccgaccc 
gaccttatc cgtagacgt ccgaatcga gaaaaaact 
ataccgagt cccggttct cctatcgca ggtggccac 
ccacacaat caagtggct agcggacga ggcggccgc 
ccgatacgc caaaaaaca atacatagt gccaacatt 
agaactcga gactaaaaa cggggcggt tttggccga 
tgacgaccc gattagtct tttgttccc aatgggggg 
aacccaggc cgaagaggt cgaggcccg cattgttaa 
atcaaatct tccgcctct tatagaccg ctacaagga 
ggcgtgtca ggacatggg ggcggtgcc acgtgactc 
gattggtca agtcctgca ggagttttc ggtggggtt 
ggatttggc caatcgggg gttcaaggg ggcggtgct 
agcccccct ggtcaaggt gttaaaccg gcaaagcaa 
cctactcga gttggactt atccggcaa ttttttaac 
aaacgagtg tttcgactt gttggccaa aagtttggc 
ctgtttgga gagcttgta caagttgaa tttgaactg 
cacaagtct cacttcggg gcatgtgca agccatcgt 
agccaactt aaacccgga cgtttcgta caaaataat 
agatctcga ctttagcga caattggtt acgtgatga 
aagcttcga aagcttggc cttgttaac acgccgatt 
acaactaaa aaagttcaa tttgtcgaa taataaatt 
cacgcaaaa cctattcaa tcaccgcct aaacaaaga 
cttgaccct aaatttgga ccaaatcga agaattcga 
catcagtga taacgcctt cgataatac cacccttat 
cactatatt atcatggct ccagttcgc caacttaac 
acacttgaa cgtgtcacg aaactctct tagtgacaa 
ctttttaca cgaagtgct caaccgaat gtggaccca 
aacgggtcc gttacgcgt ttgcaagct gtcaaattg 
tcatgtgac cgattcgga ggtgtttga tttgttgcg 
cgttgccac gtcgcttca acacttgga tgtttccta 
caatgatgc tttgacgag tatttcgtt gaggagatt 
gctacagag tggcatcat cacgttgga tatttacgc 
ttgtgggaa catttttca gtattttac cgaattttc 
taagtattt ggatgacgc gtcacgcga tcaaaatca 
aggtaagac gcttaagtt aagcccgcc ggaaaattc 
gcaacgcga gactgagta tttgatcga gatgtggag 
ggagttgta tggggagaa gacagtcgt ggaattgac 
aagtaagtt tttggggtt gtctgtatt gaaagtgta 
attttttta taaaataaa atttatttt tatatttac 
aatacaacc gtaattttt ttaacattc tgacaacaa 
gaatctaat cagtcccga attttcctt ttgtttaga 
aggtgatta cggcggtgt atacgtttt atttaatta 
caaaagttg tcttatatg gccacgtaa aataataaa 
attataaag acgactata aagtacaaa gacagaaat 
aaagaagag acttgcctt taaagccta ttttgtgca 
gttatgaaa ggcaattgc tacgtaatc gtatcacaa 
tataatgac ttacttact aaactaaga gaacctaaa 
taagtggtc cttatggca ataaatctc aatatctgt 
ttcaaaatt ctgagtcac agctaaaca aaaaaagtt 
gcataaaat ggttaaaat cacaagcgt catcaactt 
gctgaattt cgttaaaaa taatttttg catatttga 
ggcctgatt tctagaacg attaaagtt gagttaatt 
tttatctct cacctgatt ggcgttgtt atattttaa 
ctgaggttt aaatttatc tttccgtta aatatgttt 
ccacaaact gggcttaag caatttttc aggtaatta 
tttcagttt ttcaaaaaa aaatgcaaa aacttgctg 
taaaatcac aaaattgga tcgaacttg acgttattt 
gaacttgtt ttgctcgat ttggtttgt ttttaaacc 
aagaactta tttatttgg caggatatt tttctcaag 
atacactta caaattaag ataaataga ttttgctta 
tttcactct ttttttttt tgagtgaaa tttcatcaa 
aaataatcc gaaaaccag aag
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