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Gene information
General information
TC002337 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015452276.1: 231844..236814
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002337
    iBeetle screen iB_03354

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    egg chamber potentiallynumber decreased - Number of animals showing the phenotype: 4

    vitellogenic egg chamber late potentiallydegenerated - Number of animals showing the phenotype: 4

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002337 RA
      MPQETTDNP LIEKRTLYA GTKPVSFKD GTKVHFHFQ 
      TRLCDKEKT LLDDSRKMG SGKPFELVL GKKFKLEVW 
      EAVVQKMAL NEVAEFKVK KELVMQYPF VSKTLRDVA 
      LPREARKSH CCAMTIQTE GVGYDDLNT FLKDPSDLE 
      FTIELVKVE QPEDYEKES WQMDEEEKL KKVPELKEQ 
      GNAEFKKKN YEEASDYYA KAIGIIEQL MIKEKPHDV 
      EWNELDKMK VPILLNYAQ CKLNQGDYY TVIEHCTTA 
      IKSDPDNVK AYYRRAKAH VGAWNTREA FDDFKKVIE 
      LDPSLTTTV KKETAALEE QMKKKDLED KQKLKNLFS
    CDS sequence
    • >TC002337 RA
      atgccgcaa gaaaccaca gacaatcct ctaatcgag 
      aaacgcact ctctatgcg ggaacaaaa cctgtctct 
      ttcaaagat ggaacaaag gtccatttc cacttccaa 
      acacgtcta tgtgacaag gaaaagacc cttttggac 
      gactctagg aaaatgggt tcggggaaa ccatttgag 
      ctggtttta ggcaaaaaa ttcaaactg gaggtatgg 
      gaggcggtt gtgcagaaa atggctttg aatgaagtc 
      gctgagttt aaagtcaag aaagagcta gtcatgcaa 
      tatccattc gtttcgaag actttaaga gacgttgct 
      ttgccgaga gaggcaagg aaaagtcac tgttgcgct 
      atgacgatt cagactgaa ggggtgggt tacgatgac 
      ttgaatact tttttaaaa gatccctca gatttggaa 
      tttacaatt gaattagtc aaagttgaa caaccagaa 
      gattacgaa aaagaatcg tggcaaatg gacgaagag 
      gaaaaactg aaaaaagta cctgaatta aaagagcaa 
      ggaaatgca gaatttaag aaaaaaaat tacgaggaa 
      gcgtcagat tattacgcc aaagctata gggattatt 
      gaacaactc atgattaaa gaaaaacct cacgatgta 
      gaatggaac gagttggat aaaatgaaa gttccaatc 
      ttactgaat tacgctcag tgtaagcta aatcagggg 
      gattattat accgtgatt gagcactgc actactgct 
      attaaaagt gatccagat aatgtgaag gcgtactat 
      cggagggcc aaagcccac gtgggtgct tggaacact 
      cgagaggct ttcgacgat ttcaaaaaa gtgatagaa 
      ctcgatcct tccctcacc accacggtg aaaaaagag 
      acggcagct ttggaagag caaatgaag aagaaagat 
      ttggaagac aaacagaaa ttgaaaaac ttattcagt 
      taa
    mRNA sequence
    • >TC002337 RA
      tgtataatt cgctcaatt tttcgttgt tgggaggtt 
      atgttcgag acaagtcaa caaacgtca ataacttgt 
      tatcaaaat agttttatt aatttccgg gtccaattt 
      tggcaagaa taaaaaata agtatgccg caagaaacc 
      acagacaat cctctaatc gagaaacgc actctctat 
      gcgggaaca aaacctgtc tctttcaaa gatggaaca 
      aaggtccat ttccacttc caaacacgt ctatgtgac 
      aaggaaaag acccttttg gacgactct aggaaaatg 
      ggttcgggg aaaccattt gagctggtt ttaggcaaa 
      aaattcaaa ctggaggta tgggaggcg gttgtgcag 
      aaaatggct ttgaatgaa gtcgctgag tttaaagtc 
      aagaaagag ctagtcatg caatatcca ttcgtttcg 
      aagacttta agagacgtt gctttgccg agagaggca 
      aggaaaagt cactgttgc gctatgacg attcagact 
      gaaggggtg ggttacgat gacttgaat actttttta 
      aaagatccc tcagatttg gaatttaca attgaatta 
      gtcaaagtt gaacaacca gaagattac gaaaaagaa 
      tcgtggcaa atggacgaa gaggaaaaa ctgaaaaaa 
      gtacctgaa ttaaaagag caaggaaat gcagaattt 
      aagaaaaaa aattacgag gaagcgtca gattattac 
      gccaaagct atagggatt attgaacaa ctcatgatt 
      aaagaaaaa cctcacgat gtagaatgg aacgagttg 
      gataaaatg aaagttcca atcttactg aattacgct 
      cagtgtaag ctaaatcag ggggattat tataccgtg 
      attgagcac tgcactact gctattaaa agtgatcca 
      gataatgtg aaggcgtac tatcggagg gccaaagcc 
      cacgtgggt gcttggaac actcgagag gctttcgac 
      gatttcaaa aaagtgata gaactcgat ccttccctc 
      accaccacg gtgaaaaaa gagacggca gctttggaa 
      gagcaaatg aagaagaaa gatttggaa gacaaacag 
      aaattgaaa aacttattc agttaaata aatatggaa 
      aacaattta ttccgactc aaactccct taaataata 
      ctaaaatat cacattgaa ttacatgga tacacattt 
      ttggcacga tacactaat ggaatgttg gcaatactg 
      tcttacaat aaaataatt gagtggtcg cgttccagc 
      cgccgcctt gctttcagt ggaacgttg ctcatgggg 
      tccgccggt gttggcaac cctgcattg gggttggca 
      gcctggtgg taaaacacc tcagacttg ccacactga 
      tagccgtaa attacgccg tcacaacat ctgcaacaa 
      aaaattaat aaaaacaac cacttttct gttgttacc 
      aaatcgccg aattgagta actgtcgat tgtgaaatc 
      agacgattc ctcgatttt tttctgcgc gacttcggt 
      gaaccgtct aaagagcaa aatgttcaa aacagcaca 
      aataaacga cattagggc cgaaaaacc gagcgccat 
      tacaagctc gagcgggtt gtaatgaag gtcgtctat 
      gaagaaaac tgtaatgct gaggacgag atggacaac 
      tccggagta aatcgcgac gattaaaaa ggggcacag 
      cagttcgcg ggtctcgaa aaccacacc gaacacgta 
      cggcaggaa cggcattag gaaaattat ctggaagcc 
      ccagttgat ttggtcgat tcgggttaa tttgatttc 
      gaggtagta agttgtgat tattacgtt gcccactgt 
      gcacaggaa aaagattga actggcgag gaggttcat 
      aactttgag catttttag cgtgcacat tttgacaac 
      tgggtcaaa aattgacaa taataactg tgtatttca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY