Status
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Gene information
General information
TC002332 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451823.1: 1136..2876
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002332
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03353

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Fertility
    mature egg mostlynot present - Number of animals showing the phenotype: 4

    previtellogenic egg chamber not present - Number of animals showing the phenotype: 4

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002332 RA
      MSMNQYMHP RNIYKQPPN FKQLALDYP EFRKYATQD 
      VSGKVTIDF KNVGALRAL TCTLLKKDF DLNIEIPPG 
      KLIPTIPLR LNYILWLED LLNLAGGPP QARGIDIGT 
      GASCIYPLL AARKSQWSM VATEIDPES LKCATANVA 
      NNHLEGLVT VMGAQKDSL LAQVLEKFP GDFDFCMCN 
      PPFFSTPLE LHPFFKARK VKRPHPKNA FCASVDEVV 
      ATGGEVSYI SRLVEESKA WGPRVAIYT SMVGHKSSL 
      PPLKKLIRE VGAVSFKQT EFCQGHTTR WGLAWTFRD 
      VDLTKIPDS LISAKQKMA KPLLYQIPK GEDSKYKVS 
      VVCERLVGV FKELQFDFQ EIKRVRGLV EFVVVAFAD 
      TWSHQRRKR REKLRFSDG GRKCDNNNL DSPERGDVC 
      EKLAELGVG GSPSSKRAL EEDCGFYNA KKRKTESDD 
      CNSEKIYLK AKISVARVG DCIGLELVF FEGKGTKEA 
      LHQVLQFIK NNLMKD
    CDS sequence
    • >TC002332 RA
      atgtcaatg aaccagtac atgcacccc cgtaacatc 
      tacaagcag ccccccaac ttcaaacaa ctggcgtta 
      gattacccc gagtttcgc aaatacgcc actcaggac 
      gtctccggt aaagtcaca attgatttc aagaacgtg 
      ggggccctt cgggccctc acgtgcacc ctcctgaag 
      aaagacttc gatttaaat attgaaata ccccccggg 
      aaactaatc cccacaatc ccactccgc ctgaactac 
      attctatgg ctcgaagac ctcctaaac ctagccggg 
      gggcccccc caagcccgc ggcatcgac atcgggacg 
      ggcgcctcc tgcatctac ccccttctg gccgccagg 
      aagtcgcaa tggtcaatg gtcgccacc gaaatcgac 
      cccgaaagc ctcaaatgc gccaccgcc aacgtggct 
      aacaaccac ctcgagggc ttagtcacc gtcatgggg 
      gcccagaaa gacagtttg ttggcccaa gtcctggag 
      aaatttccc ggagacttt gatttttgc atgtgcaac 
      cccccgttt ttttccacg ccgctggaa ctgcacccc 
      tttttcaag gcgcgcaag gtgaagcgg ccccaccct 
      aagaacgcg ttctgcgcc tccgtggac gaagtggtg 
      gctacgggg ggcgaagtc tcgtacatt tcgcgcttg 
      gttgaggag agcaaagcg tgggggccc cgagtggcg 
      atttacacg tcgatggtg gggcacaag tcgtcgctg 
      ccgccgttg aagaagttg attagggag gtgggggct 
      gttagcttc aagcagacg gagttttgt caagggcat 
      acgacgaga tgggggcta gcgtggact ttccgtgat 
      gttgatttg accaaaatt ccagattcg ttgatcagc 
      gctaagcaa aaaatggca aagccgctc ttgtaccaa 
      atccccaag ggggaggat tccaagtat aaagtcagt 
      gttgtttgt gagagactt gttggggtt tttaaggag 
      ctgcagttt gatttccag gagattaag cgtgttagg 
      ggtttggtg gagtttgtg gtggtggcg tttgcggac 
      acgtggtcg catcagagg aggaaaagg cgcgagaag 
      ttgcgattt agcgacggg gggagaaaa tgtgataat 
      aataattta gacagtcct gagaggggt gatgtttgt 
      gaaaagttg gctgagttg ggggttggg ggctcaccg 
      tcgagcaaa cgagcgctg gaggaggat tgtgggttt 
      tataatgcg aagaaacgg aagactgag tcggatgat 
      tgtaactcg gagaagatt tatctcaag gcgaaaatt 
      agtgttgcc agagttggg gattgtatc gggttggag 
      ttggttttc tttgaaggg aagggcacg aaagaagct 
      ctacatcaa gttctgcaa tttattaaa aataatttg 
      atgaaggat taa
    mRNA sequence
    • >TC002332 RA
      ttacgcttg agtttcgtc aaggaatcg ccccaaaac 
      cgggcttaa aaaatgata atagaattg gtaattttt 
      ttgcgttgt gttggccac ccaccgcat tggaggtta 
      tgtcaattt tcaaaaaaa cgtttttga atttatttt 
      aatttaagc ttaactatt gtattttaa gagttatta 
      gagtagcga aaatgtcaa tgaaccagt acatgcacc 
      cccgtaaca tctacaagc agcccccca acttcaaac 
      aactggcgt tagattacc ccgagtttc gcaaatacg 
      ccactcagg acgtctccg gtaaagtca caattgatt 
      tcaagaacg tgggggccc ttcgggccc tcacgtgca 
      ccctcctga agaaagact tcgatttaa atattgaaa 
      taccccccg ggaaactaa tccccacaa tcccactcc 
      gcctgaact acattctat ggctcgaag acctcctaa 
      acctagccg gggggcccc cccaagccc gcggcatcg 
      acatcggga cgggcgcct cctgcatct acccccttc 
      tggccgcca ggaagtcgc aatggtcaa tggtcgcca 
      ccgaaatcg accccgaaa gcctcaaat gcgccaccg 
      ccaacgtgg ctaacaacc acctcgagg gcttagtca 
      ccgtcatgg gggcccaga aagacagtt tgttggccc 
      aagtcctgg agaaatttc ccggagact ttgattttt 
      gcatgtgca accccccgt ttttttcca cgccgctgg 
      aactgcacc cctttttca aggcgcgca aggtgaagc 
      ggccccacc ctaagaacg cgttctgcg cctccgtgg 
      acgaagtgg tggctacgg ggggcgaag tctcgtaca 
      tttcgcgct tggttgagg agagcaaag cgtgggggc 
      cccgagtgg cgatttaca cgtcgatgg tggggcaca 
      agtcgtcgc tgccgccgt tgaagaagt tgattaggg 
      aggtggggg ctgttagct tcaagcaga cggagtttt 
      gtcaagggc atacgacga gatgggggc tagcgtgga 
      ctttccgtg atgttgatt tgaccaaaa ttccagatt 
      cgttgatca gcgctaagc aaaaaatgg caaagccgc 
      tcttgtacc aaatcccca agggggagg attccaagt 
      ataaagtca gtgttgttt gtgagagac ttgttgggg 
      tttttaagg agctgcagt ttgatttcc aggagatta 
      agcgtgtta ggggtttgg tggagtttg tggtggtgg 
      cgtttgcgg acacgtggt cgcatcaga ggaggaaaa 
      ggcgcgaga agttgcgat ttagcgacg gggggagaa 
      aatgtgata ataataatt tagacagtc ctgagaggg 
      gtgatgttt gtgaaaagt tggctgagt tgggggttg 
      ggggctcac cgtcgagca aacgagcgc tggaggagg 
      attgtgggt tttataatg cgaagaaac ggaagactg 
      agtcggatg attgtaact cggagaaga tttatctca 
      aggcgaaaa ttagtgttg ccagagttg gggattgta 
      tcgggttgg agttggttt tctttgaag ggaagggca 
      cgaaagaag ctctacatc aagttctgc aatttatta 
      aaaataatt tgatgaagg attaagttt attttcacc 
      attgtatct agaataatt atttatttt agttttata 
      agagaattt atttttgtt atcgtaagg gtaataaat 
      gattgaaac agca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY