General information | |||
TC002315 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NW_015451937.1: 892046..970532 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC002315 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_03352 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
abdomen musculature specific subset dorsal partiallynot present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
remarks: muscle pattern potentially obscured by segmentation defect,
head capsule size decreased - Penetrance (percentage of animals/eggs on slide): more than 80%
head bristle pattern irregular - Penetrance (percentage of animals/eggs on slide): more than 80%
head bristle partially not present - Penetrance (percentage of animals/eggs on slide): more than 80%
labrum not present - Penetrance (percentage of animals/eggs on slide): more than 80%
dorsal ridge row irregular - Penetrance (percentage of animals/eggs on slide): more than 80%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC002315 RA
MIMEMTNES AAVGRWYES PRTGTSASP GQGTLESGN SDINTSVAE HMGAFFLDP SGTQRAARY YHQSMHSPY GTVASHVSA SRMSSGQVC RSHFHAPLH PWLSDGTKP LGHSGWVPF GGGDPDKSQ SPSSAPGQP HNIFSFPPT PPKDSTPDS VAPTAGAEY VAAAMGAFM HGGGGGDNP QSCALDVKP TAVGANANS KQREGSDYE GGTAGMFNG NFESSYGSA YGHGIHGAA YSPQNKQHL GAAGQGAQS PNKPRNKSR TSAEGRECV NCGATSTPL WRRDGTGHY LCNACGLYY KMNGQNRPL IKPKRRLSL QSAARRAGT SCANCKTTT TTLWRRNQN GEPVCNACG LYYKLHNVN RPLTMKKEG IQTRNRKLS SKSKKKKSG GSCLSLGGM MGDMMKPLD SSKGGFGGG GFGGGMGGG HPHLNAALH PHHAMSHWY QHSTHPQGF VSGPPPPAP PPTASYHHH MSSLSAAGL GLASNGMTS WRSEYT
- >TC002315 RA
atgattatg gagatgacg aacgagtct gcggcggtc ggccggtgg tacgaatcg cccaggact ggaacttcg gcgagtcct ggtcaaggg actctcgaa agtggtaat tcggacata aatactagt gttgccgaa catatgggc gcgtttttc cttgaccca tcgggcacg caaagggcc gcgagatac taccatcag agcatgcat tcgccctac gggacggtc gccagccac gtttcagcg tctcggatg tcttcgggt caagtgtgc cggtcacac ttccacgcg cccctccac ccttggcta tcggacggc acgaagccc ctcggccac tcgggttgg gtgccgttc ggtggcggc gatccggac aaatcgcag tcgccgagt tccgccccc ggccagccg cacaacatc ttctcgttc cccccgacg ccgccaaag gacagcacg ccggacagc gtggcgccg acggcgggc gcagagtac gtggcggcg gcgatgggc gccttcatg cacgggggc ggcggcggc gacaacccc cagagctgc gccctcgac gtcaagccg acggccgtc ggagcgaac gccaacagc aaacagcgc gaaggtagt gactatgaa gggggcact gccggcatg tttaatggt aactttgaa agctcgtac gggtctgcg tatggccac ggtattcac ggggccgcg tactcgccc cagaacaag cagcatctg ggggctgcc ggacagggc gcgcaatcg cctaacaaa ccacgcaac aagtcgaga accagcgcc gagggtagg gagtgcgtg aattgtggt gcaacgagc acgccactg tggcgacgt gacggcaca ggccactac ctctgcaat gcgtgtgga ttgtactac aagatgaat ggacaaaat aggccactc atcaaaccc aaaagaaga ctgagttta caaagcgct gccagaagg gccgggacc agttgtgct aactgcaag acaaccacc acaacactc tggagacgc aaccaaaac ggagagcca gtttgcaac gcctgtgga ctatactac aaactgcac aacgtgaac cgaccatta acgatgaag aaagagggc atccagacg aggaatcga aaactatcg tcgaaaagc aaaaagaag aaaagtggt ggttcctgt ctatcactc ggcggaatg atgggtgat atgatgaag ccgttggac agcagcaag ggcggtttc ggcggcggc ggttttggg ggcggcatg ggcggcgga cacccgcat ttgaacgcc gcactccat ccccatcat gccatgagc cattggtac cagcacagt acgcacccg cagggcttc gtctcgggc ccgccgccc cctgcgccg cctcccacc gcctcctac caccaccac atgagctcg ctgagcgca gcagggctg gggctagca tcgaatgga atgactagt tggcggtcg gaatacacg tga
- >TC002315 RA
tgttgttgg ccgtgttag tatcgggta gttcactta tctactgtc agaaattat tgcaacaat gacaccgtt gttttgaca aatagcgtc ggttcgaaa cccggctga ctgtgattc tgtgagtgt gtgcgagtg catgacagc cttaggatt gttacattt aagacataa cgcgtcttg gatcaccgt cagctgcgt ccgtcgcaa accaattgc tatgccgac catcaccaa cccatcgtt gtgtcccag cagcagcaa ctgaagaaa atttccgtt ttctttgcg ttgttttcc tccaataaa tcgttcgtt tttcgcgtg tgaaagtgg tgcaaaaaa actgttgta acccatgat tatggagat gacgaacga gtctgcggc ggtcggccg gtggtacga atcgcccag gactggaac ttcggcgag tcctggtca agggactct cgaaagtgg taattcgga cataaatac tagtgttgc cgaacatat gggcgcgtt tttccttga cccatcggg cacgcaaag ggccgcgag atactacca tcagagcat gcattcgcc ctacgggac ggtcgccag ccacgtttc agcgtctcg gatgtcttc gggtcaagt gtgccggtc acacttcca cgcgcccct ccacccttg gctatcgga cggcacgaa gcccctcgg ccactcggg ttgggtgcc gttcggtgg cggcgatcc ggacaaatc gcagtcgcc gagttccgc ccccggcca gccgcacaa catcttctc gttcccccc gacgccgcc aaaggacag cacgccgga cagcgtggc gccgacggc gggcgcaga gtacgtggc ggcggcgat gggcgcctt catgcacgg gggcggcgg cggcgacaa cccccagag ctgcgccct cgacgtcaa gccgacggc cgtcggagc gaacgccaa cagcaaaca gcgcgaagg tagtgacta tgaaggggg cactgccgg catgtttaa tggtaactt tgaaagctc gtacgggtc tgcgtatgg ccacggtat tcacggggc cgcgtactc gccccagaa caagcagca tctgggggc tgccggaca gggcgcgca atcgcctaa caaaccacg caacaagtc gagaaccag cgccgaggg tagggagtg cgtgaattg tggtgcaac gagcacgcc actgtggcg acgtgacgg cacaggcca ctacctctg caatgcgtg tggattgta ctacaagat gaatggaca aaataggcc actcatcaa acccaaaag aagactgag tttacaaag cgctgccag aagggccgg gaccagttg tgctaactg caagacaac caccacaac actctggag acgcaacca aaacggaga gccagtttg caacgcctg tggactata ctacaaact gcacaacgt gaaccgacc attaacgat gaagaaaga gggcatcca gacgaggaa tcgaaaact atcgtcgaa aagcaaaaa gaagaaaag tggtggttc ctgtctatc actcggcgg aatgatggg tgatatgat gaagccgtt ggacagcag caagggcgg tttcggcgg cggcggttt tgggggcgg catgggcgg cggacaccc gcatttgaa cgccgcact ccatcccca tcatgccat gagccattg gtaccagca cagtacgca cccgcaggg cttcgtctc gggcccgcc gccccctgc gccgcctcc caccgcctc ctaccacca ccacatgag ctcgctgag cgcagcagg gctggggct agcatcgaa tggaatgac tagttggcg gtcggaata cacgtgata aaatggagt aacctgaaa aacaaggaa tttaatgta agaattgta gatattaac gttaaggat ttaattaat tgaacgtga tcatgagaa aaactggtc atctgtcat tatgtacat tttatttag tttcttttt gtaaattgg gtgatttca tgaccagtt tgtatgtat aattaagta caaagatgc aaacgtttg taatgtatt gtatataga tgttagttt gttttaagg catttttaa tacaagtga tgtacatag aagaaggaa aattttaaa gacatgtgc aatcttcac gttattata aagtattta tgtgtggac gagagatat atgttggtc cgaaggtcg cactcggac cgattatac agactgcaa atatgtaaa tatggagaa atataacat tttatgagt tgtatgaaa gccgaccaa ttttattat aattaacag tttttaaat tattattca tgtgcgcaa gcattttta ttgaaaaaa tctagttta aaacaccgc gccctgtat aggatcagg acgcgctat attcaaaca cattatatt tactgattt cattcacag tacaaattc gtattaagt ttttacatt ttttaaaat actgatcca attcagggt gcaccaaag ccatgttac aatacggcg ttttgaaac gctgtgttg atttttgaa tcgattttt cgatttgta attaagtga aatagcata attaaattt tcaaaaaaa atgtaaata aaattcgat gtatgta
