General information | |||
TC002308 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NW_015451937.1: 700327..707520 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC002308 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_03349 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC002308 RA
MSSSFFHFI LVILTNLCV TQSHVSVKE VFKKDGDVN VALILHNDC NSTTVLTQQ EQSLVNSAI WTVHQLNYS EIITFGLSI YKACNEKEE YQTVFELFQ KQDENFLLG LISTSLKPQ IRKLCDALD LVIQPTVKC PTPLVEASI ELLKRLNWI ENVTVIAPN EFILNEFAI ISRKKWICV RDFVTYKSF VPSRNYTNE YIVIFANTI VFKTFVENS NTSNIIFVP EDDFVQNDL PENSYVISS KSFNTEQNV YTPTPQLFE VAIPLVNYA QNLNNENCN ETNYKQCLK TSCTVPQTI LDPTEIAKL LKIEPSSIN YFIYKSENL TLNKLFAYN LFTNNLTML EPIDLNQTM LSCLAVKDG CRQQCKNFH SQMKLISSN CDIRIRTES WIFAFLSLS LLGVLFCIA ILVFLLVSV CRKNVLEGN PVLTILLLF TVMVMFCSI VPLSLEGNR WAKRSICMA RALSITLSF AAAFSLMLS RSIVLATAS KEIGFMSHV AGPVQSFLC LFIFGVQAA LSLQVFNHC EDIFRGHSF IYILSYNII LLLLLLCFC PLIFKSQRN YREGKYFTF ATIITSLLW CFWVPAYLL LGDYYKDSV LCFGLVSTA SVLLATIFV PRTYLMTIA AARDKITST LPSLASATS AMDIYRTGA QPVYDCVNV AAINAVRVA AIQHPDLYS CPNLPEDEF DLRCETPVA DDKVTRF
- >TC002308 RA
atgagttcg agttttttc cactttatc ttagtcatc ttaacgaat ttgtgtgtc acacaatca catgttagt gttaaggaa gtattcaaa aaggacgga gacgtcaat gtagccctt attttacac aacgattgt aacagcaca acagttcta acacagcaa gagcaatcg ttggttaat tcggcaatt tggacagtg catcagttg aattattcc gaaattatc acttttggt ttgtcgatt tacaaggct tgcaacgag aaagaagaa taccaaact gtgtttgaa ctgttccaa aaacaagac gaaaatttt cttttggga ctaattagc acgtcgtta aagccacaa attcgaaaa ctttgtgat gcactagat ttggttatt caaccgact gtgaagtgt cctacaccg ttagttgaa gcttcaatt gagttgtta aaacgtcta aattggatt gaaaatgtg accgtgatt gctcccaat gaattcatt ctaaatgag tttgcaatt atttcacgg aaaaagtgg atctgcgtt agagatttt gtaacatac aagtcgttt gtaccctca cggaactac acaaacgaa tacattgta atttttgca aacactata gttttcaaa acattcgtt gaaaattcc aacacatca aatatcata tttgttcct gaggacgat tttgttcaa aatgatcta cccgaaaac agctacgtc ataagcagc aaatccttc aacaccgaa caaaatgtt tacactccc acacctcaa ctattcgaa gtcgcaatc cctcttgta aactacgct caaaactta aacaatgaa aactgcaat gaaacgaat tacaaacag tgcctcaaa accagttgc actgtcccc caaacaatt ctagaccca actgaaatc gcaaaactg ctcaaaatt gaaccgtcc agtattaat tatttcatc tacaagtct gagaacttg acactcaac aaactgttc gcctataac ctttttacg aacaactta acaatgcta gaacccatc gatttgaac caaacgatg ttaagttgc cttgcggtc aaagacggt tgtcgtcaa caatgcaaa aactttcac agccaaatg aaactaatt tcctcgaac tgcgatatt cggatacga accgaatcg tggattttt gccttcttg tcactatct ctactgggt gttttgttt tgcattgca attttagtt tttctctta gtttcagtt tgtcgtaaa aatgttctt gaaggcaat cccgtcttg acaattcta ctgttgttc actgtaatg gtcatgttt tgctcaatc gttccgttg tctttagaa ggcaatagg tgggccaaa cgttcgatt tgtatggca cgtgctttg tccataacg ctcagtttt gcagcagca ttttcccta atgctcagt cgcagcatt gttttagca acagcttcc aaagagatc gggttcatg tcacacgtt gctggtcca gttcagagt tttttgtgt ctttttatt tttggggtc caagcagct ttgagtttg caagtcttt aatcactgc gaggatatt ttcagaggc cattcgttc atttacatt ttatcatac aatataatt ttgcttctt ctcctcttg tgtttctgt cccttgatt tttaaatct cagaggaac tatcgcgag ggcaaatat ttcacgttc gccacaatt atcacctcc cttttgtgg tgtttctgg gtcccggct tatttactc ctcggtgat tattacaaa gattccgtt ctttgcttc ggtttggtg tccacagcc agtgttctc ctggccaca attttcgtt ccacgtact tatttgatg acaattgcc gcagctcgt gacaagatc acaagcact ttaccgtct cttgcttca gctacaagt gcgatggat atttacagg accggagcg cagccggtt tatgactgt gtcaatgtg gccgcaatt aatgcggta agagtggca gctattcag catccagat ctctattct tgtccgaat ttgccggaa gatgagttt gatttgagg tgcgaaacg ccggttgcg gatgataaa gtgacgagg ttttga
- >TC002308 RA
ttatttctg agtcacaca gtggggtgg atgggtttg aacaaggtc ctctattta gtccattaa aaaccgaaa aaacaagaa attgtgcat tgatgcgtt agccggaaa cggaaaagc atttgtgcc attgtattt ccatataac acacacaga cgtgaattt aggtgtcag tgaaatgag ttcgagttt tttccactt tatcttagt catcttaac gaatttgtg tgtcacaca atcacatgt tagtgttaa ggaagtatt caaaaagga cggagacgt caatgtagc ccttatttt acacaacga ttgtaacag cacaacagt tctaacaca gcaagagca atcgttggt taattcggc aatttggac agtgcatca gttgaatta ttccgaaat tatcacttt tggtttgtc gatttacaa ggcttgcaa cgagaaaga agaatacca aactgtgtt tgaactgtt ccaaaaaca agacgaaaa ttttctttt gggactaat tagcacgtc gttaaagcc acaaattcg aaaactttg tgatgcact agatttggt tattcaacc gactgtgaa gtgtcctac accgttagt tgaagcttc aattgagtt gttaaaacg tctaaattg gattgaaaa tgtgaccgt gattgctcc caatgaatt cattctaaa tgagtttgc aattatttc acggaaaaa gtggatctg cgttagaga ttttgtaac atacaagtc gtttgtacc ctcacggaa ctacacaaa cgaatacat tgtaatttt tgcaaacac tatagtttt caaaacatt cgttgaaaa ttccaacac atcaaatat catatttgt tcctgagga cgattttgt tcaaaatga tctacccga aaacagcta cgtcataag cagcaaatc cttcaacac cgaacaaaa tgtttacac tcccacacc tcaactatt cgaagtcgc aatccctct tgtaaacta cgctcaaaa cttaaacaa tgaaaactg caatgaaac gaattacaa acagtgcct caaaaccag ttgcactgt cccccaaac aattctaga cccaactga aatcgcaaa actgctcaa aattgaacc gtccagtat taattattt catctacaa gtctgagaa cttgacact caacaaact gttcgccta taacctttt tacgaacaa cttaacaat gctagaacc catcgattt gaaccaaac gatgttaag ttgccttgc ggtcaaaga cggttgtcg tcaacaatg caaaaactt tcacagcca aatgaaact aatttcctc gaactgcga tattcggat acgaaccga atcgtggat ttttgcctt cttgtcact atctctact gggtgtttt gttttgcat tgcaatttt agtttttct cttagtttc agtttgtcg taaaaatgt tcttgaagg caatcccgt cttgacaat tctactgtt gttcactgt aatggtcat gttttgctc aatcgttcc gttgtcttt agaaggcaa taggtgggc caaacgttc gatttgtat ggcacgtgc tttgtccat aacgctcag ttttgcagc agcattttc cctaatgct cagtcgcag cattgtttt agcaacagc ttccaaaga gatcgggtt catgtcaca cgttgctgg tccagttca gagtttttt gtgtctttt tatttttgg ggtccaagc agctttgag tttgcaagt ctttaatca ctgcgagga tattttcag aggccattc gttcattta cattttatc atacaatat aattttgct tcttctcct cttgtgttt ctgtccctt gatttttaa atctcagag gaactatcg cgagggcaa atatttcac gttcgccac aattatcac ctccctttt gtggtgttt ctgggtccc ggcttattt actcctcgg tgattatta caaagattc cgttctttg cttcggttt ggtgtccac agccagtgt tctcctggc cacaatttt cgttccacg tacttattt gatgacaat tgccgcagc tcgtgacaa gatcacaag cactttacc gtctcttgc ttcagctac aagtgcgat ggatattta caggaccgg agcgcagcc ggtttatga ctgtgtcaa tgtggccgc aattaatgc ggtaagagt ggcagctat tcagcatcc agatctcta ttcttgtcc gaatttgcc ggaagatga gtttgattt gaggtgcga aacgccggt tgcggatga taaagtgac gaggttttg aggtcatga attgtttgc acaataatg gaaaaagga caggatgtg taatttaca ttacgaact gtttccgtc aaagggatc agattaaaa cattatttt atcgtggca tcaactcac cctggagaa agtatacag tgttattca cgtgagacg tcgagcgtt ttaaaaatt aaattaaac taagctgat ttttaataa atctatttt tcgagcgtt atatttaaa attgtgtaa agaatgtat tttgattgt atattactt actttttta aggctcgac atgttacgc gaatagcac tgtagataa ttgtcaagg tcacaaatc cagatttta ggttgagaa gagtaaatt atttagggt gtgtatatt atttaaaac ctttataaa ataagtgtc agtactaat tgtaaaaac aatacaaat tttatgtga aa
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
