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Gene information
General information
TC002299 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451937.1: 200900..202959
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002299
    iBeetle screen iB_03348

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002299 RA
      MSDGPPETL KKFVFSSKY NLKGEPQEE 
      QLEVIIPEL LQVSYSFYT WPSAPVLAW 
      FLWENRQQL TGKKILEIG SGTALPGIV 
      AAKCGAKVI LSDSTTLPK SLNHTKRSC 
      QLNNLVLNE DIHIIGLTW GLFLDNLEL 
      IGELDLILG SDCFYEPSV FEDVLVSVS 
      YLLDLNQGA KFLCTYQER SSDWSIEHL 
      LAKWNLNCQ VHNISNLGA SAGLDIHRL 
      VGDHSIHLL EISRQI
    CDS sequence
    • >TC002299 RA
      atgtctgat ggacctcct gaaacgtta aaaaagttc 
      gtgttttcg tccaaatat aacctcaaa ggcgagcct 
      caggaagaa caactcgaa gtaatcatt cctgagctt 
      cttcaagtc agctattcc ttctacact tggccttcg 
      gcacccgtc cttgcgtgg ttcctgtgg gagaacaga 
      caacagctc accggaaag aaaattcta gagatcggt 
      tctgggacc gctttgcct ggaatcgtg gccgcaaag 
      tgtggagcg aaggtaatt ctaagcgat tctaccact 
      cttcctaaa tctctgaat cacacaaag aggagttgt 
      cagttgaat aatctggtt ttgaatgaa gatattcac 
      ataattgga ctgacgtgg gggttgttt ttggacaat 
      ttggagcta attggcgaa cttgacctg attttgggt 
      tcggattgt ttttacgaa ccttctgtt ttcgaggac 
      gtgttggtc tcagtttcg tatctgctc gacttaaat 
      caaggggcc aagtttttg tgcacatat caggagcgt 
      agttctgac tggtccatt gagcatctt ctggccaag 
      tggaatttg aattgtcaa gtgcataac atcagcaat 
      ttgggggct agtgctggg ttggatata cataggtta 
      gttggggac catagcata catttgttg gaaatctct 
      agacaaatt tga
    mRNA sequence
    • >TC002299 RA
      cttgtttca taaaacgac aaataataa ataaccata 
      atcatttcg aggaataaa cccgttttt tatggttgt 
      tacataatt tgggaattt ttccaatac caacataag 
      ctgtcaatt tccggcaaa aaacgcgag gtaagattt 
      gcgattttg ggattgcgt gttttataa aaactgctt 
      gttgtgtgc gtaaataat gcctaaacc gcttgttgt 
      cactttccc aaatgtctg atggacctc ctgaaacgt 
      taaaaaagt tcgtgtttt cgtccaaat ataacctca 
      aaggcgagc ctcaggaag aacaactcg aagtaatca 
      ttcctgagc ttcttcaag tcagctatt ccttctaca 
      cttggcctt cggcacccg tccttgcgt ggttcctgt 
      gggagaaca gacaacagc tcaccggaa agaaaattc 
      tagagatcg gttctggga ccgctttgc ctggaatcg 
      tggccgcaa agtgtggag cgaaggtaa ttctaagcg 
      attctacca ctcttccta aatctctga atcacacaa 
      agaggagtt gtcagttga ataatctgg ttttgaatg 
      aagatattc acataattg gactgacgt gggggttgt 
      ttttggaca atttggagc taattggcg aacttgacc 
      tgattttgg gttcggatt gtttttacg aaccttctg 
      ttttcgagg acgtgttgg tctcagttt cgtatctgc 
      tcgacttaa atcaagggg ccaagtttt tgtgcacat 
      atcaggagc gtagttctg actggtcca ttgagcatc 
      ttctggcca agtggaatt tgaattgtc aagtgcata 
      acatcagca atttggggg ctagtgctg ggttggata 
      tacataggt tagttgggg accatagca tacatttgt 
      tggaaatct ctagacaaa tttgattat ggctggtaa 
      tgtcgcacg aaatttcta taaggtcta cgtggggaa 
      tctgccttg gacaattgg tcatcttga actgaagaa 
      ctacttcaa caaatacgg tcaagtcgc cgttgctaa 
      cgtaatctt tgataaaga tacaggcct gtcacggaa 
      ttacggatt tgtttttta ccgtaataa ggacgaatt 
      tgataaggc gttaaacac tgaaggaca caaattaga 
      aggcaacac gctgaaagt ctttcccgc gaatcaaac 
      acagtcaga tcaatagtc atggcaact tctttagat 
      agttttaac gacctcaaa gaactaggg atcgattgc 
      gaagatagc gattcttta acagacgca tttaacaaa 
      gctgctaat cacctacca aatctttta cccaatgta 
      gataatcac actctttta tctttgtac ggttactat 
      aaacaaggt tcgcaagga ccatgcaat acacctaag 
      ccaagttgg tttgacatg aaagctaaa tccaaatgg 
      gaagcgtgg agtaaacta ggagacatg cctcaaaat 
      aaagccaaa cagatttac atcgaaact ataaaaact 
      ttagacccg acttttaac gttccggaa aaggaaagt 
      tgggtttct gtttccgta ccgcaaaat ttagaagga 
      ttcaaagaa gctaaggag aaaactgtt gctgatttt 
      gtcaaagag gaaaattac ttggaagtt gctaaacta 
      ttggagtca ccatcaata gcaaaggat ttgataaac 
      aagcttgat gaagaagga ttgggtttg attcattgg 
      gctgcagat agaggatcg gttgatatt ttagaactt 
      ctgttcaag tgcggtgcc aatgtcgac cttcaagat 
      tccgacggg caaactgct ttacactac gcttcgagt 
      tgtggccat ttggaatgt attaaaata cttttaact 
      tgtaatgca agacgggat atcgtcgat ggagaaggt 
      ttgagtcca gaggatgta gcttgtgat gacgctgtt 
      aaagaattg ttagtcaat tttaagtag tctttgtta 
      taataaata tctagctaa ata
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY