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Gene information
General information
TC002297 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451937.1: 192801..194722
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002297
    iBeetle screen iB_03347

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002297 RA
      MTEESEPGM KILLMDKET TSVISMVYG QSEIQQKEV 
      FLLERLDSP NQSNSLGLR YLKCLVFLR PTQENIGLL 
      CNELRYPKY GAYYIYFSN IVAKADIKI LAEHDEQEV 
      VKEVQELYM DYLAVNPHL FSIGLPTCM NSLSWNPTA 
      LQRTVQGIV AVLLSLKKS PAIRYQANS KLCKDLGTR 
      IDEVMNKES SLFAFGQNS HPLLLILDR RDDPMTPLL 
      NQWTYQAMV HELLTINNN RVNLSGIPG VGKELSEVV 
      LSAEQDPFY AKNIFLNYG EIGQNIKQL MDQFQAKAK 
      SHQKIESIA DMKNFVEAY PQFKKLSGN VTKHVTVVG 
      ELSSMVNKY HLLDVSEIE QEISSHNDH SSHLQSIKK 
      LINNDKVRN TDIAKLVML YALRYQNHS NNDVVGLIE 
      LLKKRGVSE RLIKNIVNI LEYAGSHAR QSDFLNVEN 
      ALQSTKRFF KGLSGVDNV YTQHKPLIH ETLEELVKG 
      RLKDSLYPY VGNHFLNGR PQDIIIFMV GGTTYEESL 
      TVHSFNKSN PSFNIALGG TTIHNSTSF LAEVEQATR 
      NLPRKHTRN IRNVRFD
    CDS sequence
    • >TC002297 RA
      atgaccgaa gagagcgaa cccggaatg aaaatctta 
      ttaatggac aaggagaca accagtgtc atcagtatg 
      gtttacggc cagtctgaa attcagcaa aaagaggtt 
      ttcctgctc gagagattg gattcacca aaccagagc 
      aattcgttg gggctgcgc tatctaaaa tgtttagtc 
      ttcctccgc cccacgcag gaaaacatt gggttattg 
      tgtaatgaa ttgcgttat cccaagtac ggcgcttac 
      tacatctac ttcagcaac attgtggcc aaagctgac 
      atcaagatt ttagctgaa catgatgag caagaagtt 
      gttaaggaa gttcaggag ttgtacatg gattattta 
      gctgtgaac cctcatctg ttttcaata ggtttacca 
      acttgcatg aacagtctg tcttggaac cctacagct 
      ctccaaaga acagttcaa ggaattgtt gctgttctc 
      ctctcattg aagaagagt ccagcaatc agatatcaa 
      gccaattca aagctttgc aaagacctt ggaacacga 
      attgacgaa gttatgaat aaagaatcg tcattgttt 
      gcgttcggc caaaacagc caccctttg ttgctcatt 
      ttggaccga agagatgac ccaatgact cctctccta 
      aaccagtgg acatatcaa gctatggta catgaacta 
      ttaacaatc aataataat cgcgtcaac ttatcggga 
      atccctgga gtcggtaaa gaattatct gaagtggtt 
      ttatcagca gaacaggac ccattttat gccaaaaat 
      atttttctg aattacggc gaaattgga cagaatatc 
      aaacaacta atggatcag ttccaagcc aaagccaag 
      agccaccaa aaaattgaa agtatcgcg gatatgaag 
      aattttgtt gaagcttat ccacaattt aagaaactc 
      tcaggaaac gttactaaa catgtgaca gtcgttggt 
      gagttaagt tcaatggtc aataaatac cacctactt 
      gatgtgtct gaaattgag caggagatt tcctcacat 
      aatgaccac tcatcacat ttgcaaagc atcaagaag 
      ctaataaac aatgataaa gttagaaat acagatatt 
      gcaaaatta gtgatgctt tatgcgtta cgctatcaa 
      aatcacagc aacaatgac gtggtggga cttattgaa 
      ttgctaaaa aagagagga gtttccgaa cgtcttatt 
      aagaatatt gtcaatata ttggaatat gcgggttca 
      cacgctaga caaagtgat tttctcaac gtggaaaac 
      gcccttcaa agcacgaag cggttcttt aagggatta 
      agtggagtt gataatgta tacacgcaa cataaacct 
      ttaatccat gaaaccctt gaagaattg gttaaggga 
      cgattgaaa gacagcctt tatccctac gttggaaat 
      cattttctc aacgggaga cctcaagac atcatcatt 
      tttatggtc ggaggcaca acctacgag gagtcttta 
      actgtacat tccttcaac aaaagtaat ccgagcttt 
      aatattgct cttggaggc acaacaatc cataattct 
      acgagcttt ttagctgaa gttgaacaa gctacgaga 
      aatctaccc agaaaacat accagaaat atccgtaat 
      gttagattt gactaa
    mRNA sequence
    • >TC002297 RA
      tctagtttg acgtctcga aaatcgatt ccaatttga 
      agaaagatg ataaagccg cttttacct acttctgtt 
      atttaattg ttatgccaa gagttagtt attaaagat 
      gaacctaat agcagcagt aaaggcata tgtcaccaa 
      aatgaccga agagagcga acccggaat gaaaatctt 
      attaatgga caaggagac aaccagtgt catcagtat 
      ggtttacgg ccagtctga aattcagca aaaagaggt 
      tttcctgct cgagagatt ggattcacc aaaccagag 
      caattcgtt ggggctgcg ctatctaaa atgtttagt 
      cttcctccg ccccacgca ggaaaacat tgggttatt 
      gtgtaatga attgcgtta tcccaagta cggcgctta 
      ctacatcta cttcagcaa cattgtggc caaagctga 
      catcaagat tttagctga acatgatga gcaagaagt 
      tgttaagga agttcagga gttgtacat ggattattt 
      agctgtgaa ccctcatct gttttcaat aggtttacc 
      aacttgcat gaacagtct gtcttggaa ccctacagc 
      tctccaaag aacagttca aggaattgt tgctgttct 
      cctctcatt gaagaagag tccagcaat cagatatca 
      agccaattc aaagctttg caaagacct tggaacacg 
      aattgacga agttatgaa taaagaatc gtcattgtt 
      tgcgttcgg ccaaaacag ccacccttt gttgctcat 
      tttggaccg aagagatga cccaatgac tcctctcct 
      aaaccagtg gacatatca agctatggt acatgaact 
      attaacaat caataataa tcgcgtcaa cttatcggg 
      aatccctgg agtcggtaa agaattatc tgaagtggt 
      tttatcagc agaacagga cccatttta tgccaaaaa 
      tatttttct gaattacgg cgaaattgg acagaatat 
      caaacaact aatggatca gttccaagc caaagccaa 
      gagccacca aaaaattga aagtatcgc ggatatgaa 
      gaattttgt tgaagctta tccacaatt taagaaact 
      ctcaggaaa cgttactaa acatgtgac agtcgttgg 
      tgagttaag ttcaatggt caataaata ccacctact 
      tgatgtgtc tgaaattga gcaggagat ttcctcaca 
      taatgacca ctcatcaca tttgcaaag catcaagaa 
      gctaataaa caatgataa agttagaaa tacagatat 
      tgcaaaatt agtgatgct ttatgcgtt acgctatca 
      aaatcacag caacaatga cgtggtggg acttattga 
      attgctaaa aaagagagg agtttccga acgtcttat 
      taagaatat tgtcaatat attggaata tgcgggttc 
      acacgctag acaaagtga ttttctcaa cgtggaaaa 
      cgcccttca aagcacgaa gcggttctt taagggatt 
      aagtggagt tgataatgt atacacgca acataaacc 
      tttaatcca tgaaaccct tgaagaatt ggttaaggg 
      acgattgaa agacagcct ttatcccta cgttggaaa 
      tcattttct caacgggag acctcaaga catcatcat 
      ttttatggt cggaggcac aacctacga ggagtcttt 
      aactgtaca ttccttcaa caaaagtaa tccgagctt 
      taatattgc tcttggagg cacaacaat ccataattc 
      tacgagctt tttagctga agttgaaca agctacgag 
      aaatctacc cagaaaaca taccagaaa tatccgtaa 
      tgttagatt tgactaatt tcggtacag acttatgag 
      aattgtata ttttattta tttatgttg ttatgtaat 
      aaataataa ttgattatc taataaaag gataataaa 
      gcattttga gtgtg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY