Status
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Gene information
General information
TC002294 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451937.1: 65803..69556
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC002294
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03345

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002294 RA
      MSTQSANPR EPTSHVNSI SKSKILVVG AGGIGCEIL 
KNLCVSGFQ DIEVIDLDT IDVSNLNRQ FLFRKEHVG 
KSKAVVARE SIISFNSNV NIKAYHDSI FNQEYGVNF 
FKRFDLVLN ALDNRAARN HVNRMCLAA DIPLIESGT 
AGYSGQVEL IKKGMTQCY ECQPKPQQK SYPGCTIRN 
TPSEPVHCI VWAKHLFNQ LFGEEDPDQ DVSPDTEDP 
EVKKDGAFS VTESGDVKR KSTRQWAQE VDYNPEQLF 
NKFFKDDIN YLLSMENLW KTRKPPVPI SWEKALTCE 
SRISNDEES SKSLDMQVL SISKYTQIF ASTVNILKK 
ELAGKKFLM WDKDDTPAM DFVTACANI RAFIFSISQ 
KSKFEIKSI AGNIIPAIA TANALIAGA AVLYALRVL 
QNDYEKCPT IYLRQKSIY SKVLLATDK SLQKPNPNC 
YVCSAKPVV NVFVNVNKM LVKEFETEV LKNTLNMVA 
PDAILDGKG VVVISSEEG ETEINNKKT LCEVGIVDG 
SILKVDDFL QNYELIINV NQYEAKEKD DPPFKFIAN 
PAELKAKQI DGVKNGVTA AKNDLPNQE MHTDNDDVV 
ICTTDNDDF GASSSKKRK LNPVEDDDD DLIILDEQL 
E
    CDS sequence
    • >TC002294 RA
      atgagcact caatctgca aatcctcgt gaaccaact 
tctcatgta aattcaatt tctaaatca aaaatactg 
gttgttgga gcaggaggg atcggatgt gaaatactg 
aaaaatttg tgtgtttcg ggctttcaa gatattgaa 
gtgattgat ttagacaca attgatgtc agcaattta 
aatcgtcag tttctgttt cgcaaagaa catgtcggg 
aagtccaaa gcagtagtt gctagagaa agcataata 
tcttttaat tccaacgtg aatattaaa gcatatcac 
gacagtatc tttaaccaa gaatatggg gttaatttt 
tttaaaaga tttgatctg gttttgaat gcgttagat 
aacagagct gcacgcaat cacgttaat cgtatgtgc 
ttagctgct gacatccct ttaattgaa tcaggaact 
gcgggatat tcaggacaa gtggaactc atcaaaaag 
ggaatgaca caatgctat gaatgccaa ccaaaaccc 
cagcaaaag agttatccc ggttgtact attcggaac 
actccatct gaaccagtc cactgcatt gtttgggct 
aaacatctg tttaaccaa ctatttggt gaagaagat 
cctgaccaa gatgtctca cccgatacc gaagatcca 
gaggtaaaa aaagacggt gctttttct gtaactgag 
tctggagat gtgaaaaga aaatcaaca cgtcagtgg 
gcacaggaa gtggattat aatcctgaa cagcttttt 
aacaagttt ttcaaagac gatattaat tatttacta 
tctatggaa aatctctgg aaaacacgt aagccgccg 
gttcctatt tcttgggag aaggcactt acctgtgag 
tcccggatt tctaacgat gaagaaagt tcaaaatcc 
cttgacatg caagttttg tcaataagc aagtacact 
caaattttt gcgagtaca gtgaacatt ttgaagaag 
gaactcgct ggtaaaaag tttcttatg tgggataag 
gacgatacg cccgccatg gattttgta acagcttgt 
gccaacata cgtgcattt atattttcc atttcccag 
aagtcaaaa tttgaaatt aaatcaatt gcaggaaac 
atcattcca gcaattgcc actgcaaat gctttaatt 
gctggtgct gctgtcttg tacgctctc agagtattg 
caaaatgat tatgaaaaa tgtcctaca atatatttg 
cggcaaaaa tcaatttat tcaaaagtt ttgttagca 
acagataag agcttgcaa aaacctaat cctaattgt 
tacgtatgc tctgcaaaa ccagtcgtc aacgtattt 
gttaatgtg aataagatg ttagtcaaa gaatttgaa 
actgaagtt ttgaaaaac acacttaat atggtggct 
ccagacgct attttagat ggtaaaggg gttgtagtt 
atttcatct gaagaagga gaaacggag atcaacaac 
aaaaaaaca ctttgcgaa gttggaatc gtggatgga 
agtattttg aaagtggat gactttctg caaaattat 
gaattgatt ataaacgtt aaccagtat gaagctaaa 
gaaaaagat gatccacca tttaaattt attgcaaat 
cctgccgag cttaaagca aagcagatc gacggtgtc 
aagaatgga gtcaccgct gctaaaaac gatttacca 
aatcaagaa atgcacaca gataatgat gatgtcgtt 
atatgcaca acagataat gatgatttt ggagcttca 
tcaagtaaa aagcgtaaa ttgaatcct gttgaagat 
gatgatgat gatcttata atactagat gaacaactt 
gagtag
    mRNA sequence
    • >TC002294 RA
      cgtaaagtg tacccgaaa ggtttcggc attcggcgt 
tgttttgta aagtcttgt tggttatct gtcgtccgt 
tattataac attagatta aatcgacaa attggcagc 
attaaagtg tttcaagtg aaaaagtat tagtacaac 
tttgtctct caagatcct tacataatg agcactcaa 
tctgcaaat cctcgtgaa ccaacttct catgtaaat 
tcaatttct aaatcaaaa atactggtt gttggagca 
ggagggatc ggatgtgaa atactgaaa aatttgtgt 
gtttcgggc tttcaagat attgaagtg attgattta 
gacacaatt gatgtcagc aatttaaat cgtcagttt 
ctgtttcgc aaagaacat gtcgggaag tccaaagca 
gtagttgct agagaaagc ataatatct tttaattcc 
aacgtgaat attaaagca tatcacgac agtatcttt 
aaccaagaa tatggggtt aattttttt aaaagattt 
gatctggtt ttgaatgcg ttagataac agagctgca 
cgcaatcac gttaatcgt atgtgctta gctgctgac 
atcccttta attgaatca ggaactgcg ggatattca 
ggacaagtg gaactcatc aaaaaggga atgacacaa 
tgctatgaa tgccaacca aaaccccag caaaagagt 
tatcccggt tgtactatt cggaacact ccatctgaa 
ccagtccac tgcattgtt tgggctaaa catctgttt 
aaccaacta tttggtgaa gaagatcct gaccaagat 
gtctcaccc gataccgaa gatccagag gtaaaaaaa 
gacggtgct ttttctgta actgagtct ggagatgtg 
aaaagaaaa tcaacacgt cagtgggca caggaagtg 
gattataat cctgaacag ctttttaac aagtttttc 
aaagacgat attaattat ttactatct atggaaaat 
ctctggaaa acacgtaag ccgccggtt cctatttct 
tgggagaag gcacttacc tgtgagtcc cggatttct 
aacgatgaa gaaagttca aaatccctt gacatgcaa 
gttttgtca ataagcaag tacactcaa atttttgcg 
agtacagtg aacattttg aagaaggaa ctcgctggt 
aaaaagttt cttatgtgg gataaggac gatacgccc 
gccatggat tttgtaaca gcttgtgcc aacatacgt 
gcatttata ttttccatt tcccagaag tcaaaattt 
gaaattaaa tcaattgca ggaaacatc attccagca 
attgccact gcaaatgct ttaattgct ggtgctgct 
gtcttgtac gctctcaga gtattgcaa aatgattat 
gaaaaatgt cctacaata tatttgcgg caaaaatca 
atttattca aaagttttg ttagcaaca gataagagc 
ttgcaaaaa cctaatcct aattgttac gtatgctct 
gcaaaacca gtcgtcaac gtatttgtt aatgtgaat 
aagatgtta gtcaaagaa tttgaaact gaagttttg 
aaaaacaca cttaatatg gtggctcca gacgctatt 
ttagatggt aaaggggtt gtagttatt tcatctgaa 
gaaggagaa acggagatc aacaacaaa aaaacactt 
tgcgaagtt ggaatcgtg gatggaagt attttgaaa 
gtggatgac tttctgcaa aattatgaa ttgattata 
aacgttaac cagtatgaa gctaaagaa aaagatgat 
ccaccattt aaatttatt gcaaatcct gccgagctt 
aaagcaaag cagatcgac ggtgtcaag aatggagtc 
accgctgct aaaaacgat ttaccaaat caagaaatg 
cacacagat aatgatgat gtcgttata tgcacaaca 
gataatgat gattttgga gcttcatca agtaaaaag 
cgtaaattg aatcctgtt gaagatgat gatgatgat 
cttataata ctagatgaa caacttgag tagtttcaa 
gaaattatg ttgtcgatg tttttttta tatccggaa 
ataaactta tttggagaa t
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