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Gene information
General information
TC002294 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451937.1: 65803..69556
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002294
    iBeetle screen iB_03345

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002294 RA
      MSTQSANPR EPTSHVNSI SKSKILVVG AGGIGCEIL 
      KNLCVSGFQ DIEVIDLDT IDVSNLNRQ FLFRKEHVG 
      KSKAVVARE SIISFNSNV NIKAYHDSI FNQEYGVNF 
      FKRFDLVLN ALDNRAARN HVNRMCLAA DIPLIESGT 
      AGYSGQVEL IKKGMTQCY ECQPKPQQK SYPGCTIRN 
      TPSEPVHCI VWAKHLFNQ LFGEEDPDQ DVSPDTEDP 
      EVKKDGAFS VTESGDVKR KSTRQWAQE VDYNPEQLF 
      NKFFKDDIN YLLSMENLW KTRKPPVPI SWEKALTCE 
      SRISNDEES SKSLDMQVL SISKYTQIF ASTVNILKK 
      ELAGKKFLM WDKDDTPAM DFVTACANI RAFIFSISQ 
      KSKFEIKSI AGNIIPAIA TANALIAGA AVLYALRVL 
      QNDYEKCPT IYLRQKSIY SKVLLATDK SLQKPNPNC 
      YVCSAKPVV NVFVNVNKM LVKEFETEV LKNTLNMVA 
      PDAILDGKG VVVISSEEG ETEINNKKT LCEVGIVDG 
      SILKVDDFL QNYELIINV NQYEAKEKD DPPFKFIAN 
      PAELKAKQI DGVKNGVTA AKNDLPNQE MHTDNDDVV 
      ICTTDNDDF GASSSKKRK LNPVEDDDD DLIILDEQL 
      E
    CDS sequence
    • >TC002294 RA
      atgagcact caatctgca aatcctcgt gaaccaact 
      tctcatgta aattcaatt tctaaatca aaaatactg 
      gttgttgga gcaggaggg atcggatgt gaaatactg 
      aaaaatttg tgtgtttcg ggctttcaa gatattgaa 
      gtgattgat ttagacaca attgatgtc agcaattta 
      aatcgtcag tttctgttt cgcaaagaa catgtcggg 
      aagtccaaa gcagtagtt gctagagaa agcataata 
      tcttttaat tccaacgtg aatattaaa gcatatcac 
      gacagtatc tttaaccaa gaatatggg gttaatttt 
      tttaaaaga tttgatctg gttttgaat gcgttagat 
      aacagagct gcacgcaat cacgttaat cgtatgtgc 
      ttagctgct gacatccct ttaattgaa tcaggaact 
      gcgggatat tcaggacaa gtggaactc atcaaaaag 
      ggaatgaca caatgctat gaatgccaa ccaaaaccc 
      cagcaaaag agttatccc ggttgtact attcggaac 
      actccatct gaaccagtc cactgcatt gtttgggct 
      aaacatctg tttaaccaa ctatttggt gaagaagat 
      cctgaccaa gatgtctca cccgatacc gaagatcca 
      gaggtaaaa aaagacggt gctttttct gtaactgag 
      tctggagat gtgaaaaga aaatcaaca cgtcagtgg 
      gcacaggaa gtggattat aatcctgaa cagcttttt 
      aacaagttt ttcaaagac gatattaat tatttacta 
      tctatggaa aatctctgg aaaacacgt aagccgccg 
      gttcctatt tcttgggag aaggcactt acctgtgag 
      tcccggatt tctaacgat gaagaaagt tcaaaatcc 
      cttgacatg caagttttg tcaataagc aagtacact 
      caaattttt gcgagtaca gtgaacatt ttgaagaag 
      gaactcgct ggtaaaaag tttcttatg tgggataag 
      gacgatacg cccgccatg gattttgta acagcttgt 
      gccaacata cgtgcattt atattttcc atttcccag 
      aagtcaaaa tttgaaatt aaatcaatt gcaggaaac 
      atcattcca gcaattgcc actgcaaat gctttaatt 
      gctggtgct gctgtcttg tacgctctc agagtattg 
      caaaatgat tatgaaaaa tgtcctaca atatatttg 
      cggcaaaaa tcaatttat tcaaaagtt ttgttagca 
      acagataag agcttgcaa aaacctaat cctaattgt 
      tacgtatgc tctgcaaaa ccagtcgtc aacgtattt 
      gttaatgtg aataagatg ttagtcaaa gaatttgaa 
      actgaagtt ttgaaaaac acacttaat atggtggct 
      ccagacgct attttagat ggtaaaggg gttgtagtt 
      atttcatct gaagaagga gaaacggag atcaacaac 
      aaaaaaaca ctttgcgaa gttggaatc gtggatgga 
      agtattttg aaagtggat gactttctg caaaattat 
      gaattgatt ataaacgtt aaccagtat gaagctaaa 
      gaaaaagat gatccacca tttaaattt attgcaaat 
      cctgccgag cttaaagca aagcagatc gacggtgtc 
      aagaatgga gtcaccgct gctaaaaac gatttacca 
      aatcaagaa atgcacaca gataatgat gatgtcgtt 
      atatgcaca acagataat gatgatttt ggagcttca 
      tcaagtaaa aagcgtaaa ttgaatcct gttgaagat 
      gatgatgat gatcttata atactagat gaacaactt 
      gagtag
    mRNA sequence
    • >TC002294 RA
      cgtaaagtg tacccgaaa ggtttcggc attcggcgt 
      tgttttgta aagtcttgt tggttatct gtcgtccgt 
      tattataac attagatta aatcgacaa attggcagc 
      attaaagtg tttcaagtg aaaaagtat tagtacaac 
      tttgtctct caagatcct tacataatg agcactcaa 
      tctgcaaat cctcgtgaa ccaacttct catgtaaat 
      tcaatttct aaatcaaaa atactggtt gttggagca 
      ggagggatc ggatgtgaa atactgaaa aatttgtgt 
      gtttcgggc tttcaagat attgaagtg attgattta 
      gacacaatt gatgtcagc aatttaaat cgtcagttt 
      ctgtttcgc aaagaacat gtcgggaag tccaaagca 
      gtagttgct agagaaagc ataatatct tttaattcc 
      aacgtgaat attaaagca tatcacgac agtatcttt 
      aaccaagaa tatggggtt aattttttt aaaagattt 
      gatctggtt ttgaatgcg ttagataac agagctgca 
      cgcaatcac gttaatcgt atgtgctta gctgctgac 
      atcccttta attgaatca ggaactgcg ggatattca 
      ggacaagtg gaactcatc aaaaaggga atgacacaa 
      tgctatgaa tgccaacca aaaccccag caaaagagt 
      tatcccggt tgtactatt cggaacact ccatctgaa 
      ccagtccac tgcattgtt tgggctaaa catctgttt 
      aaccaacta tttggtgaa gaagatcct gaccaagat 
      gtctcaccc gataccgaa gatccagag gtaaaaaaa 
      gacggtgct ttttctgta actgagtct ggagatgtg 
      aaaagaaaa tcaacacgt cagtgggca caggaagtg 
      gattataat cctgaacag ctttttaac aagtttttc 
      aaagacgat attaattat ttactatct atggaaaat 
      ctctggaaa acacgtaag ccgccggtt cctatttct 
      tgggagaag gcacttacc tgtgagtcc cggatttct 
      aacgatgaa gaaagttca aaatccctt gacatgcaa 
      gttttgtca ataagcaag tacactcaa atttttgcg 
      agtacagtg aacattttg aagaaggaa ctcgctggt 
      aaaaagttt cttatgtgg gataaggac gatacgccc 
      gccatggat tttgtaaca gcttgtgcc aacatacgt 
      gcatttata ttttccatt tcccagaag tcaaaattt 
      gaaattaaa tcaattgca ggaaacatc attccagca 
      attgccact gcaaatgct ttaattgct ggtgctgct 
      gtcttgtac gctctcaga gtattgcaa aatgattat 
      gaaaaatgt cctacaata tatttgcgg caaaaatca 
      atttattca aaagttttg ttagcaaca gataagagc 
      ttgcaaaaa cctaatcct aattgttac gtatgctct 
      gcaaaacca gtcgtcaac gtatttgtt aatgtgaat 
      aagatgtta gtcaaagaa tttgaaact gaagttttg 
      aaaaacaca cttaatatg gtggctcca gacgctatt 
      ttagatggt aaaggggtt gtagttatt tcatctgaa 
      gaaggagaa acggagatc aacaacaaa aaaacactt 
      tgcgaagtt ggaatcgtg gatggaagt attttgaaa 
      gtggatgac tttctgcaa aattatgaa ttgattata 
      aacgttaac cagtatgaa gctaaagaa aaagatgat 
      ccaccattt aaatttatt gcaaatcct gccgagctt 
      aaagcaaag cagatcgac ggtgtcaag aatggagtc 
      accgctgct aaaaacgat ttaccaaat caagaaatg 
      cacacagat aatgatgat gtcgttata tgcacaaca 
      gataatgat gattttgga gcttcatca agtaaaaag 
      cgtaaattg aatcctgtt gaagatgat gatgatgat 
      cttataata ctagatgaa caacttgag tagtttcaa 
      gaaattatg ttgtcgatg tttttttta tatccggaa 
      ataaactta tttggagaa t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY