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Gene information
General information
TC002287 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451937.1: 80367..94762
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002287
    iBeetle screen iB_03343

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002287 RA
      MEKEPQPDS MATITIKPE YPPSEIYSS EPPPAYHRS 
      NSSAVQVAK IIAVTVVLV SVVLGSFLL ASAYITATA 
      SCRQLEQEL ELLNEAADR FQPPLSPEA LVREDPQKQ 
      SSNDLETKE SRSQENENK NKSVDSTSS ESSEDSESD 
      SSSSDNGES DEKTVLLKL PLHLDFDDL GALLEKSRK 
      PKINCVVEK KRAEEIVDH KPKALNLPF GLNLTTDPR 
      YERISGERI AIFCDSGNE QKQSPQEDD QVEETMLIQ 
      PVMIPIPQT HFPTHMAQQ FAQRPPYPM ETPMSPRVQ 
      QIREQNQLP PNQILHQIA QEVIAQKIM EMQRVREGQ 
      NQEMPQFNS ESVAQKAPI PDEVLAQLN RFPNRDVIV 
      TVSQEFDDG LQELNEGQA DPQKGAKTN FQQMNAAPQ 
      QMHTVPPSQ NQRAGPDMR AQPQLVMSE VQQAAPMEM 
      LNVPPVVAE ALQELKVYE QQRQNAQER NDRQMYERN 
      PTAVPMRMM PPGLDSQTT ASEEPRPHY VQPRSVRSV 
      DALLPKVEK RVKRCSCDC AC
    CDS sequence
    • >TC002287 RA
      atggagaag gaaccgcaa cctgattcg atggcaact 
      atcactatt aaacctgag tatccacca tctgaaata 
      tattcttca gaaccacct ccggcgtac caccgttct 
      aatagttca gcagttcaa gtggcaaaa atcattgca 
      gtaacagtt gtcctggtg tctgttgtg ttaggtagt 
      ttcttacta gcttctgct tacattacg gcgactgct 
      tcatgccga cagttggaa caagagttg gaactactc 
      aatgaagca gccgacagg tttcaacct cctttatca 
      ccagaagca cttgtgcgg gaagatcca caaaaacaa 
      agctcaaat gatttagaa actaaagaa tcccgaagc 
      caagaaaac gagaacaag aataaaagt gttgactcc 
      acaagttct gaaagttct gaagactct gaaagcgat 
      tcctccagt agtgacaat ggagaatcg gatgaaaaa 
      acagttttg ctaaagtta cctttacat ttagacttt 
      gatgacttg ggagcatta cttgaaaaa agtagaaaa 
      cccaagatc aattgtgta gttgagaaa aaacgtgcc 
      gaagaaatt gttgatcat aaacccaaa gctctcaat 
      ttacccttt ggactgaac ttaacaact gatccaaga 
      tacgaacgc atcagcggt gagagaata gcgatcttc 
      tgtgatagc ggtaacgaa caaaaacaa tcaccccaa 
      gaagatgac caagtggaa gaaaccatg ttgatacaa 
      ccggttatg atcccaatt ccccaaact cacttcccc 
      acacacatg gctcaacag ttcgcacaa aggccaccc 
      tacccaatg gaaaccccc atgtcccca cgagttcaa 
      caaatcaga gaacaaaac caattgccg cctaaccaa 
      attctgcat caaatcgca caggaagtt atcgctcag 
      aaaattatg gaaatgcaa agagtgcga gaaggccaa 
      aatcaggaa atgccgcaa ttcaactct gaatcagtg 
      gcgcaaaaa gctcccata cctgatgaa gtccttgca 
      cagttgaac aggttccca aatcgcgat gttattgta 
      actgtttca caagaattt gatgatgga ttgcaagaa 
      ctgaatgaa ggccaagcc gatcctcag aaaggagca 
      aaaaccaat tttcaacaa atgaatgct gctccacag 
      caaatgcac acagttcct ccatcacag aaccaaaga 
      gctggaccc gatatgcgg gcacaacca cagttggtg 
      atgtcagaa gtacaacaa gccgctcca atggaaatg 
      cttaatgtc ccgcctgtt gttgctgaa gctttgcag 
      gagctgaag gtttatgaa cagcaaaga caaaacgca 
      caagaaagg aatgacaga cagatgtat gagagaaat 
      ccaacagca gttcctatg cgtatgatg cctcctgga 
      ctcgattct caaaccact gcttcggaa gagcctaga 
      cctcactat gttcagcca agatctgtg cgttcggtg 
      gacgctcta cttcccaag gttgaaaag agagtcaaa 
      cgctgttcc tgtgattgt gcttgttaa
    mRNA sequence
    • >TC002287 RA
      attgtaata tttaaagtc agctgattt tgaacccat 
      atcccatat ccatgctaa ccacgtcag attaactca 
      tgttctaga tttattcca ccatttact ttccagttc 
      cattgcgtt tcaaaggtg gggaatcaa acagactgg 
      gagatggac aggaaagat ccactacca ttatccctg 
      tcagtcgat ttcagttgc tagtggagt gagcattct 
      tattttaaa tatttaata ttttaacaa agggattag 
      ttttgtact gtgattacc taaaacaat ggagaagga 
      accgcaacc tgattcgat ggcaactat cactattaa 
      acctgagta tccaccatc tgaaatata ttcttcaga 
      accacctcc ggcgtacca ccgttctaa tagttcagc 
      agttcaagt ggcaaaaat cattgcagt aacagttgt 
      cctggtgtc tgttgtgtt aggtagttt cttactagc 
      ttctgctta cattacggc gactgcttc atgccgaca 
      gttggaaca agagttgga actactcaa tgaagcagc 
      cgacaggtt tcaacctcc tttatcacc agaagcact 
      tgtgcggga agatccaca aaaacaaag ctcaaatga 
      tttagaaac taaagaatc ccgaagcca agaaaacga 
      gaacaagaa taaaagtgt tgactccac aagttctga 
      aagttctga agactctga aagcgattc ctccagtag 
      tgacaatgg agaatcgga tgaaaaaac agttttgct 
      aaagttacc tttacattt agactttga tgacttggg 
      agcattact tgaaaaaag tagaaaacc caagatcaa 
      ttgtgtagt tgagaaaaa acgtgccga agaaattgt 
      tgatcataa acccaaagc tctcaattt accctttgg 
      actgaactt aacaactga tccaagata cgaacgcat 
      cagcggtga gagaatagc gatcttctg tgatagcgg 
      taacgaaca aaaacaatc accccaaga agatgacca 
      agtggaaga aaccatgtt gatacaacc ggttatgat 
      cccaattcc ccaaactca cttccccac acacatggc 
      tcaacagtt cgcacaaag gccacccta cccaatgga 
      aacccccat gtccccacg agttcaaca aatcagaga 
      acaaaacca attgccgcc taaccaaat tctgcatca 
      aatcgcaca ggaagttat cgctcagaa aattatgga 
      aatgcaaag agtgcgaga aggccaaaa tcaggaaat 
      gccgcaatt caactctga atcagtggc gcaaaaagc 
      tcccatacc tgatgaagt ccttgcaca gttgaacag 
      gttcccaaa tcgcgatgt tattgtaac tgtttcaca 
      agaatttga tgatggatt gcaagaact gaatgaagg 
      ccaagccga tcctcagaa aggagcaaa aaccaattt 
      tcaacaaat gaatgctgc tccacagca aatgcacac 
      agttcctcc atcacagaa ccaaagagc tggacccga 
      tatgcgggc acaaccaca gttggtgat gtcagaagt 
      acaacaagc cgctccaat ggaaatgct taatgtccc 
      gcctgttgt tgctgaagc tttgcagga gctgaaggt 
      ttatgaaca gcaaagaca aaacgcaca agaaaggaa 
      tgacagaca gatgtatga gagaaatcc aacagcagt 
      tcctatgcg tatgatgcc tcctggact cgattctca 
      aaccactgc ttcggaaga gcctagacc tcactatgt 
      tcagccaag atctgtgcg ttcggtgga cgctctact 
      tcccaaggt tgaaaagag agtcaaacg ctgttcctg 
      tgattgtgc ttgttaaga agtaaaagc taaaatcca 
      ctttgttgc tccgtactt cataactac aatagtttt 
      ttattttac taatgtaaa atggcttat atggaaata 
      gtgtaaata gagcttgaa tactaatgt agtttccaa 
      tagatagta acggcagta gtttttttg tgaattaat 
      tgtaaaatg ctttataat tatggtata gtctttatt 
      tttggactt tttgtgtaa acacatttg caataaata 
      gttacacca ttattgtat gacaatttt agaaataaa 
      cagtgtccc aatgattgg aacattgcc ttcatttaa 
      attgtaccg tgttctgca gttgaaaat acacttttt 
      agaccttc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY