Status
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Gene information
General information
TC002285 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451937.1: 109176..166314
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002285
    iBeetle screen iB_03342

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    iBeetle screen iB_06665

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    low validity due to technical reasons - should be repeated: musculature not scorable,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002285 RA
      MLLLLVIVP VVICVSDNF LKMFEGQKP PVDPCYDED 
      RPRRCIPDF VNAAFGIPV KASSTCGLH SPVQFCEVS 
      EPEGNSQCY ICDDKLPKN RFPASHLTD LNNPNNVTC 
      WRSDPLLPV TSINAAPDN VTLVLSLGK KYELTYVSL 
      QFCPNTPKP DSIAIYKSM DYGKTWQAF QFYSTQCRR 
      VYGRPNKAT ITKVNEQEA RCTDAHRYT GGDNQGFGQ 
      SSRIAFSTL EGRPSALDF DNSPVLQDW ITATDIKII 
      FNRLYMPLE DASSENFDT LVDELKNKQ TDDDLNFTK 
      EPVVQLVNE MQPLDTNDP KTPTSVELT SNGVAGGYN 
      QITHQYAVA DFAVGGRCK CNGHASRCI TGRDGQLAC 
      ECKHNTAGK DCERCKPFH FDRPWGRAT ARDANECKA 
      CECNQHARR CRFNMELYK LSGRVSGGV CLKCRHYTA 
      GRHCHYCRE GFYRDPTKQ ITHRKACKP CDCHPIGSS 
      GRTCNQATG QCPCKDGVV GTTCNRCAK GYQQSRSHI 
      APCIKIPVV QIMATEEED NDYGDEDPD QPAAGVQCG 
      KCKSSTKRL NLNKYCKRD YAIMARVLS RMEGNEDQH 
      TKGWTRFMV NIEFIYKKS RDSQIRKGT MPMVVPTAD 
      LACKCPKLK PSKSYLFLG REKDDSPGG VLTGSLGVT 
      ERSIVIEWR DEWDQRMRR FRRRARKCK
    CDS sequence
    • >TC002285 RA
      atgttattg ttactcgta attgtgccc gttgtgatt 
      tgtgtttct gataacttt ctcaagatg ttcgagggg 
      caaaaaccc ccggttgac ccctgctac gacgaagac 
      cgcccccgt cgttgcatt ccagacttt gtaaatgca 
      gcgttcggt atcccagta aaagcgtct tctacctgc 
      ggtcttcat tcacctgtg caattttgc gaagtttcg 
      gaaccagag ggcaattcc cagtgctac atatgcgac 
      gacaaattg ccaaagaat cgatttcca gcatctcac 
      ctcacagat cttaacaat cccaacaac gtgacttgt 
      tggaggtct gacccgctg cttccagtg acttccatt 
      aatgctgca ccagataac gtaacgttg gttctcagt 
      ttaggaaag aagtacgag ctgacgtat gtaagcctg 
      cagttttgc cccaacaca cctaagccc gattcaatc 
      gcaatctac aaaagcatg gactacggg aagacatgg 
      caagcgttt cagttctat tcgactcag tgtcgcaga 
      gtttacggc agaccaaac aaagcaacc attactaaa 
      gtcaatgaa caggaggca cgatgtact gatgctcat 
      cggtatacg ggaggtgat aatcaaggt ttcggacag 
      tcgtccaga atagcgttc agtacactt gagggccgc 
      ccaagtgct ttggatttc gacaactcg ccggtgctc 
      caagactgg atcaccgcc accgatatt aaaatcatt 
      tttaatcgc ctttacatg cctctagag gatgcgtct 
      tccgaaaac ttcgataca ctcgttgac gaattaaaa 
      aacaaacaa acagacgat gatcttaat tttaccaaa 
      gaaccggtc gtccagttg gtgaacgaa atgcaaccc 
      ttagacacg aatgatccc aaaacaccc acatcagtg 
      gaattgact tcaaatggg gtagccggt ggttacaac 
      caaattact caccagtat gccgtcgcc gattttgcc 
      gtcggagga agatgcaaa tgtaatgga catgcaagt 
      cgctgcata acaggtaga gatggacaa ttagcatgt 
      gagtgtaaa cataatacg gctggaaag gattgcgaa 
      agatgtaaa ccattccat tttgataga ccttggggc 
      agagccact gctcgggac gcaaatgaa tgcaaagct 
      tgtgaatgc aaccaacat gctcgtcgt tgtcgattc 
      aacatggaa ctttataaa ctgtccgga agagtttcc 
      ggtggcgtt tgtttaaaa tgtcgacac tatacagca 
      ggaagacat tgtcattat tgcagagaa ggtttttat 
      agagatcct acaaaacaa attactcat cgtaaagca 
      tgtaaacct tgtgactgc caccctatt ggatcttcc 
      ggtagaacc tgcaatcaa gccacagga caatgtcca 
      tgtaaagat ggtgtggtt gggactacc tgcaataga 
      tgtgcaaaa ggttaccaa caaagccga tcccatatt 
      gcaccgtgc ataaaaatt cctgtagtg caaataatg 
      gctaccgaa gaagaagac aatgattat ggagacgaa 
      gatccagat caacctgca gctggagtt caatgtgga 
      aaatgcaaa tccagcacg aagagatta aacttgaat 
      aaatattgt aaaagagac tatgctatc atggcgaga 
      gtcttgtcg cgaatggaa ggtaatgaa gaccaacat 
      acaaaagga tggacgcga tttatggtg aatatagaa 
      ttcatctac aaaaaatca agggattct caaatacgt 
      aaaggcact atgcctatg gtcgtgcca acagcagat 
      ctggcttgt aaatgtcct aaactcaaa cctagcaag 
      tcctacctg tttttagga agagagaaa gacgattct 
      cccggagga gttcttacg ggtagttta ggtgtcaca 
      gaaagatca attgtcatt gaatggaga gatgagtgg 
      gatcaaagg atgcgaaga tttcgtcga agagctcgc 
      aaatgcaag taa
    mRNA sequence
    • >TC002285 RA
      acgcatttc gcagtaaat ttaaattgc aataatttg 
      atacgaggt cggagcaga gcagacagc ggaccgcca 
      gttattcaa cggcagtcg gagtgaggg aagtgttcc 
      tcgaattgg acacatcgc acgcggact aataaatca 
      aaactatcg gcaacgctg ttttcgaaa tcgatgtgt 
      gaaaaacgt gtgtctaaa aacatgaaa tcgtttgat 
      tgctaagtt gtgcgattc attcataac atgtgcagt 
      gcagtgaat atgttattg ttactcgta attgtgccc 
      gttgtgatt tgtgtttct gataacttt ctcaagatg 
      ttcgagggg caaaaaccc ccggttgac ccctgctac 
      gacgaagac cgcccccgt cgttgcatt ccagacttt 
      gtaaatgca gcgttcggt atcccagta aaagcgtct 
      tctacctgc ggtcttcat tcacctgtg caattttgc 
      gaagtttcg gaaccagag ggcaattcc cagtgctac 
      atatgcgac gacaaattg ccaaagaat cgatttcca 
      gcatctcac ctcacagat cttaacaat cccaacaac 
      gtgacttgt tggaggtct gacccgctg cttccagtg 
      acttccatt aatgctgca ccagataac gtaacgttg 
      gttctcagt ttaggaaag aagtacgag ctgacgtat 
      gtaagcctg cagttttgc cccaacaca cctaagccc 
      gattcaatc gcaatctac aaaagcatg gactacggg 
      aagacatgg caagcgttt cagttctat tcgactcag 
      tgtcgcaga gtttacggc agaccaaac aaagcaacc 
      attactaaa gtcaatgaa caggaggca cgatgtact 
      gatgctcat cggtatacg ggaggtgat aatcaaggt 
      ttcggacag tcgtccaga atagcgttc agtacactt 
      gagggccgc ccaagtgct ttggatttc gacaactcg 
      ccggtgctc caagactgg atcaccgcc accgatatt 
      aaaatcatt tttaatcgc ctttacatg cctctagag 
      gatgcgtct tccgaaaac ttcgataca ctcgttgac 
      gaattaaaa aacaaacaa acagacgat gatcttaat 
      tttaccaaa gaaccggtc gtccagttg gtgaacgaa 
      atgcaaccc ttagacacg aatgatccc aaaacaccc 
      acatcagtg gaattgact tcaaatggg gtagccggt 
      ggttacaac caaattact caccagtat gccgtcgcc 
      gattttgcc gtcggagga agatgcaaa tgtaatgga 
      catgcaagt cgctgcata acaggtaga gatggacaa 
      ttagcatgt gagtgtaaa cataatacg gctggaaag 
      gattgcgaa agatgtaaa ccattccat tttgataga 
      ccttggggc agagccact gctcgggac gcaaatgaa 
      tgcaaagct tgtgaatgc aaccaacat gctcgtcgt 
      tgtcgattc aacatggaa ctttataaa ctgtccgga 
      agagtttcc ggtggcgtt tgtttaaaa tgtcgacac 
      tatacagca ggaagacat tgtcattat tgcagagaa 
      ggtttttat agagatcct acaaaacaa attactcat 
      cgtaaagca tgtaaacct tgtgactgc caccctatt 
      ggatcttcc ggtagaacc tgcaatcaa gccacagga 
      caatgtcca tgtaaagat ggtgtggtt gggactacc 
      tgcaataga tgtgcaaaa ggttaccaa caaagccga 
      tcccatatt gcaccgtgc ataaaaatt cctgtagtg 
      caaataatg gctaccgaa gaagaagac aatgattat 
      ggagacgaa gatccagat caacctgca gctggagtt 
      caatgtgga aaatgcaaa tccagcacg aagagatta 
      aacttgaat aaatattgt aaaagagac tatgctatc 
      atggcgaga gtcttgtcg cgaatggaa ggtaatgaa 
      gaccaacat acaaaagga tggacgcga tttatggtg 
      aatatagaa ttcatctac aaaaaatca agggattct 
      caaatacgt aaaggcact atgcctatg gtcgtgcca 
      acagcagat ctggcttgt aaatgtcct aaactcaaa 
      cctagcaag tcctacctg tttttagga agagagaaa 
      gacgattct cccggagga gttcttacg ggtagttta 
      ggtgtcaca gaaagatca attgtcatt gaatggaga 
      gatgagtgg gatcaaagg atgcgaaga tttcgtcga 
      agagctcgc aaatgcaag taaattaac tcgtacgtt 
      aatgaaagt tactaaatt gttccatac attttgtaa 
      aagatgcta gcagtgtca ctgtgattt aattttaac 
      gtatctgtt taagagact taaagcatt ttaagggcg 
      cgtttaaaa gtacactac ctcatttta tcattatca 
      gtttttcct ttagagaat aaagaaatc aaaatatt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY