Status
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Gene information
General information
TC002272 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451937.1: 292610..299082
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002272
    iBeetle screen iB_03340

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002272 RA
      MGAKISTGE KIQPREFTE KFQRSESEA SDDFKTKQR 
      STTDACFLI FLSLFLTVL LCFLGYCIC NGDIFRIIN 
      GYDNCGNVC GRYNVIHHG EIRDEVCQE SSTWSKPYH 
      IVIRSNDSS VIERKCVSD CNDNGGYRK FFNRCVPVQ 
      STKVVNTFF SKTGIKNFF QEVSEDFHL CWPEFLYLC 
      LIALAFSVL ILFLFRYVV GIVVWVVLI GVVLACCIA 
      TIILWILWN QSRSNRPEK LISDVDFRK TGTYLAFAI 
      IATVVTVIV ILVIIVMRK RIELVVQLF KEAGKAVAA 
      VPLLLLQPI LTFLSLAVV ITLWFYFCL WIESSGYLT 
      EKKPHVYYY EKDKWMVIT RWYNLLAML WMCQFVIGC 
      QHMVIAGTV SDWYFTRNK DELGTPILK SGYNLVRYH 
      LGSVALGSF LIAIVQFVR VILKCAEKY LHSHRGKCV 
      DYLLKCCHC CLYCFEKIL KYMSRNAYI EIAMYGYSF 
      CQAGRQAFR LLVANVLRV TAINSVGDF VLFLGKVLV 
      VAATVLIGT KMLQNKEGL QHMWVPLTL AGLFAYFVA 
      HCFMTVYEM AIDTIFLCF CEDCEQNDG IAKPYYMSR 
      GLMEFVENS KKVLESHDC RQKGSQAWQ EKVPSISDS 
      VEKKI
    • >TC002272 RB
      MGAKISTGE KIQPRETFL SLAVVITLW FYFCLWIES 
      SGYLTEKKP HVYYYEKDK WMVITRWYN LLAMLWMCQ 
      FVIGCQHMV IAGTVSDWY FTRNKDELG TPILKSGYN 
      LVRYHLGSV ALGSFLIAI VQFVRVILK CAEKYLHSH 
      RGKCVDYLL KCCHCCLYC FEKILKYMS RNAYIEIAM 
      YGYSFCQAG RQAFRLLVA NVLRVTAIN SVGDFVLFL 
      GKVLVVAAT VLIGTKMLQ NKEGLQHMW VPLTLAGLF 
      AYFVAHCFM TVYEMAIDT IFLCFCEDC EQNDGIAKP 
      YYMSRGLME FVENSKKVL ESHDCRQKG SQAWQEKVP 
      SISDSVEKK I
    CDS sequence
    • >TC002272 RA
      atgggggca aaaataagc acgggggaa aaaattcag 
      cctcgagag ttcactgaa aaatttcaa cgttcagaa 
      tcagaagcc agcgatgat tttaaaacc aaacaacgg 
      tctaccacc gatgcgtgc ttcctgatt tttctttcc 
      ctatttttg actgttttg ctatgtttc ctgggttat 
      tgcatttgc aatggtgat attttccga attattaac 
      ggttatgat aattgtgga aacgtgtgt ggaaggtac 
      aatgtgatt catcacgga gaaataagg gatgaagtc 
      tgtcaagaa tcctcgact tggtctaag ccttaccac 
      attgtaata cggagtaac gattcatcg gttattgaa 
      aggaaatgt gtctcagac tgtaacgat aatggagga 
      taccgaaag tttttcaac agatgtgtt cctgttcaa 
      agtactaaa gtggtcaat acgtttttt tcaaaaact 
      ggaataaaa aatttcttt caagaagtt tcagaggac 
      ttccattta tgttggcct gaatttcta tacctttgt 
      cttattgct ttggcattc tctgttttg attctgttc 
      ttattccga tacgtggta ggaatagtg gtgtgggtg 
      gtactaata ggtgtcgta ctggcctgt tgtattgcc 
      acaataatt ttatggatt ttgtggaac cagtccaga 
      agtaatcgt ccggaaaaa ttgatttct gacgttgat 
      tttagaaaa actggtact tatttagca tttgcgata 
      attgccact gtggtaacc gtgattgta attctggtt 
      atcattgtg atgcgaaaa agaatcgaa ttggtcgtc 
      cagttattc aaagaagca ggaaaagca gtggcagca 
      gtgcctctt cttcttcta caacccatc ctaactttt 
      ctttcgctt gctgtggta atcactctt tggttttac 
      ttttgtttg tggattgaa agttcggga taccttact 
      gagaagaaa cctcatgtt tactattac gagaaagat 
      aagtggatg gtaatcacc agatggtac aatttgttg 
      gcaatgctg tggatgtgc cagtttgtg atagggtgc 
      caacatatg gtaatagct ggtactgtg tccgattgg 
      tactttact agaaacaag gatgaactt ggcactcct 
      attctcaaa agtgggtac aacttggtc aggtaccat 
      cttggttca gtagccttg ggctctttt ctcatcgct 
      atagttcag tttgtccga gtgatttta aaatgtgct 
      gagaaatat cttcacagt cacagaggc aaatgtgtt 
      gactattta ttgaaatgt tgccattgt tgtctatac 
      tgctttgaa aagattttg aaatatatg agtcgaaat 
      gcgtacatt gaaattgca atgtacggc tactccttt 
      tgtcaagca ggccgacaa gcgtttaga cttcttgta 
      gcaaacgtt ctccgagta acagcaata aattcagtt 
      ggagatttc gttctcttt ttaggaaaa gttcttgta 
      gttgctgca acggtgtta atcgggaca aaaatgcta 
      cagaacaaa gaaggattg cagcatatg tgggttcct 
      ttgacccta gctggcctt ttcgcctat tttgttgct 
      cattgtttc atgacagtc tacgaaatg gcaattgat 
      acgatattt ctgtgtttt tgtgaagac tgtgaacag 
      aatgatggt attgctaaa ccctattac atgtcgcga 
      ggcttaatg gagtttgtt gagaattcc aaaaaagtt 
      ttagaatcg cacgactgc agacaaaaa gggagtcaa 
      gcgtggcaa gaaaaagtt ccatcaata agtgattcg 
      gttgaaaag aaaatttaa
    • >TC002272 RB
      atgggggca aaaataagc acgggggaa aaaattcag 
      cctcgagag acttttctt tcgcttgct gtggtaatc 
      actctttgg ttttacttt tgtttgtgg attgaaagt 
      tcgggatac cttactgag aagaaacct catgtttac 
      tattacgag aaagataag tggatggta atcaccaga 
      tggtacaat ttgttggca atgctgtgg atgtgccag 
      tttgtgata gggtgccaa catatggta atagctggt 
      actgtgtcc gattggtac tttactaga aacaaggat 
      gaacttggc actcctatt ctcaaaagt gggtacaac 
      ttggtcagg taccatctt ggttcagta gccttgggc 
      tcttttctc atcgctata gttcagttt gtccgagtg 
      attttaaaa tgtgctgag aaatatctt cacagtcac 
      agaggcaaa tgtgttgac tatttattg aaatgttgc 
      cattgttgt ctatactgc tttgaaaag attttgaaa 
      tatatgagt cgaaatgcg tacattgaa attgcaatg 
      tacggctac tccttttgt caagcaggc cgacaagcg 
      tttagactt cttgtagca aacgttctc cgagtaaca 
      gcaataaat tcagttgga gatttcgtt ctcttttta 
      ggaaaagtt cttgtagtt gctgcaacg gtgttaatc 
      gggacaaaa atgctacag aacaaagaa ggattgcag 
      catatgtgg gttcctttg accctagct ggccttttc 
      gcctatttt gttgctcat tgtttcatg acagtctac 
      gaaatggca attgatacg atatttctg tgtttttgt 
      gaagactgt gaacagaat gatggtatt gctaaaccc 
      tattacatg tcgcgaggc ttaatggag tttgttgag 
      aattccaaa aaagtttta gaatcgcac gactgcaga 
      caaaaaggg agtcaagcg tggcaagaa aaagttcca 
      tcaataagt gattcggtt gaaaagaaa atttaa
    mRNA sequence
    • >TC002272 RA
      ggaaattcg agggtgaca gtggaagtt tgtaaagcg 
      ggtttaaat agaaaatgg gcttatatt atagtctaa 
      gtttaggtg atgaccgct aaggaagaa tttaaattg 
      cttgattgg tttggaatg gtgggttat atcacaatg 
      cacttgtaa attgttgtt acactgcga agaattgca 
      aatgatcca ttttggaac ctctcagga aaacacttt 
      ctttcagat gacatgggg gcaaaaata agcacgggg 
      gaaaaaatt cagcctcga gagttcact gaaaaattt 
      caacgttca gaatcagaa gccagcgat gattttaaa 
      accaaacaa cggtctacc accgatgcg tgcttcctg 
      atttttctt tccctattt ttgactgtt ttgctatgt 
      ttcctgggt tattgcatt tgcaatggt gatattttc 
      cgaattatt aacggttat gataattgt ggaaacgtg 
      tgtggaagg tacaatgtg attcatcac ggagaaata 
      agggatgaa gtctgtcaa gaatcctcg acttggtct 
      aagccttac cacattgta atacggagt aacgattca 
      tcggttatt gaaaggaaa tgtgtctca gactgtaac 
      gataatgga ggataccga aagtttttc aacagatgt 
      gttcctgtt caaagtact aaagtggtc aatacgttt 
      ttttcaaaa actggaata aaaaatttc tttcaagaa 
      gtttcagag gacttccat ttatgttgg cctgaattt 
      ctatacctt tgtcttatt gctttggca ttctctgtt 
      ttgattctg ttcttattc cgatacgtg gtaggaata 
      gtggtgtgg gtggtacta ataggtgtc gtactggcc 
      tgttgtatt gccacaata attttatgg attttgtgg 
      aaccagtcc agaagtaat cgtccggaa aaattgatt 
      tctgacgtt gattttaga aaaactggt acttattta 
      gcatttgcg ataattgcc actgtggta accgtgatt 
      gtaattctg gttatcatt gtgatgcga aaaagaatc 
      gaattggtc gtccagtta ttcaaagaa gcaggaaaa 
      gcagtggca gcagtgcct cttcttctt ctacaaccc 
      atcctaact tttctttcg cttgctgtg gtaatcact 
      ctttggttt tacttttgt ttgtggatt gaaagttcg 
      ggatacctt actgagaag aaacctcat gtttactat 
      tacgagaaa gataagtgg atggtaatc accagatgg 
      tacaatttg ttggcaatg ctgtggatg tgccagttt 
      gtgataggg tgccaacat atggtaata gctggtact 
      gtgtccgat tggtacttt actagaaac aaggatgaa 
      cttggcact cctattctc aaaagtggg tacaacttg 
      gtcaggtac catcttggt tcagtagcc ttgggctct 
      tttctcatc gctatagtt cagtttgtc cgagtgatt 
      ttaaaatgt gctgagaaa tatcttcac agtcacaga 
      ggcaaatgt gttgactat ttattgaaa tgttgccat 
      tgttgtcta tactgcttt gaaaagatt ttgaaatat 
      atgagtcga aatgcgtac attgaaatt gcaatgtac 
      ggctactcc ttttgtcaa gcaggccga caagcgttt 
      agacttctt gtagcaaac gttctccga gtaacagca 
      ataaattca gttggagat ttcgttctc tttttagga 
      aaagttctt gtagttgct gcaacggtg ttaatcggg 
      acaaaaatg ctacagaac aaagaagga ttgcagcat 
      atgtgggtt cctttgacc ctagctggc cttttcgcc 
      tattttgtt gctcattgt ttcatgaca gtctacgaa 
      atggcaatt gatacgata tttctgtgt ttttgtgaa 
      gactgtgaa cagaatgat ggtattgct aaaccctat 
      tacatgtcg cgaggctta atggagttt gttgagaat 
      tccaaaaaa gttttagaa tcgcacgac tgcagacaa 
      aaagggagt caagcgtgg caagaaaaa gttccatca 
      ataagtgat tcggttgaa aagaaaatt taattatgt 
      gattgtaat tttgaaatg cacagttgt atgtggttt 
      tatattttt cagtacgca catatttgt atgtatcta 
      ttttagaag aagatattt gaatatatt ttaaagact 
      ttt
    • >TC002272 RB
      ggaaattcg agggtgaca gtggaagtt tgtaaagcg 
      ggtttaaat agaaaatgg gcttatatt atagtctaa 
      gtttaggtg atgaccgct aaggaagaa tttaaattg 
      cttgattgg tttggaatg gtgggttat atcacaatg 
      cacttgtaa attgttgtt acactgcga agaattgca 
      aatgatcca ttttggaac ctctcagga aaacacttt 
      ctttcagat gacatgggg gcaaaaata agcacgggg 
      gaaaaaatt cagcctcga gagactttt ctttcgctt 
      gctgtggta atcactctt tggttttac ttttgtttg 
      tggattgaa agttcggga taccttact gagaagaaa 
      cctcatgtt tactattac gagaaagat aagtggatg 
      gtaatcacc agatggtac aatttgttg gcaatgctg 
      tggatgtgc cagtttgtg atagggtgc caacatatg 
      gtaatagct ggtactgtg tccgattgg tactttact 
      agaaacaag gatgaactt ggcactcct attctcaaa 
      agtgggtac aacttggtc aggtaccat cttggttca 
      gtagccttg ggctctttt ctcatcgct atagttcag 
      tttgtccga gtgatttta aaatgtgct gagaaatat 
      cttcacagt cacagaggc aaatgtgtt gactattta 
      ttgaaatgt tgccattgt tgtctatac tgctttgaa 
      aagattttg aaatatatg agtcgaaat gcgtacatt 
      gaaattgca atgtacggc tactccttt tgtcaagca 
      ggccgacaa gcgtttaga cttcttgta gcaaacgtt 
      ctccgagta acagcaata aattcagtt ggagatttc 
      gttctcttt ttaggaaaa gttcttgta gttgctgca 
      acggtgtta atcgggaca aaaatgcta cagaacaaa 
      gaaggattg cagcatatg tgggttcct ttgacccta 
      gctggcctt ttcgcctat tttgttgct cattgtttc 
      atgacagtc tacgaaatg gcaattgat acgatattt 
      ctgtgtttt tgtgaagac tgtgaacag aatgatggt 
      attgctaaa ccctattac atgtcgcga ggcttaatg 
      gagtttgtt gagaattcc aaaaaagtt ttagaatcg 
      cacgactgc agacaaaaa gggagtcaa gcgtggcaa 
      gaaaaagtt ccatcaata agtgattcg gttgaaaag 
      aaaatttaa ttatgtgat tgtaatttt gaaatgcac 
      agttgtatg tggttttat atttttcag tacgcacat 
      atttgtatg tatctattt tagaagaag atatttgaa 
      tatatttta aagactttt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY