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Gene information
General information
TC002268 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451937.1: 447594..457441
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002268
    iBeetle screen iB_03338

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 4 number 2nd lateral oviduct: 5 - Number of animals showing the phenotype: 4
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002268 RA
      MENLRLYVG TYNVGTSTP DIKLNSLLG LPDDHKTDK 
      LQLPDIYIL SFQEVKAQP QNMLLDALF DDPWTYACK 
      ELLDTFNYV KIKSVRLQG LLLNVYCLR KHLLNIREI 
      ESDYTRTGL SGMWGNKGA VSVRLSIYG CSLCFVNSH 
      LSAHDNQLK DRVEDYNNI IKDQEFHVE ETTEIFFHD 
      YVFWMGDLN FRLLEEYDQ TPEEIERDI VKNELKKLF 
      TFDQLRYVM RKGEAFSEL EEKDPEFPP TFKFEVGTN 
      RYDFKRRPA WCDRILYRV NPHNYENVT LKVDQLSYK 
      HHPSYQLSD HKPVTAEFN IKVFSDYSE RIVEFDKIV 
      SWNQDEENK ARYKITKDI PATKDDWIG LFKDNFSSL 
      DDYVSYEYV SKCASPTPE KKSPMKGGS SKNEQKYEI 
      TFSELPNRC RGNYCLVYF SQSEDKVIS VLGISNSFP 
      IVKHDSD
    CDS sequence
    • >TC002268 RA
      atggaaaat ttgcgatta tatgtgggc acctacaat 
      gtgggtaca agcaccccc gatataaag ctaaactcg 
      ttattaggg ttgcctgat gaccacaag acagataaa 
      ttgcagcta ccagatatt tacattttg agttttcaa 
      gaagtgaaa gcgcagcct caaaacatg cttcttgat 
      gcgctattt gacgacccc tggacttac gcgtgcaaa 
      gaacttctt gacaccttc aactatgtt aaaatcaag 
      tcagttagg cttcagggt ttgttgctc aatgtttat 
      tgtctcagg aaacaccta ctcaatatt agggaaatt 
      gagtctgac tacaccagg acagggctg tcaggaatg 
      tggggtaat aaaggtgca gttagtgtt agactcagt 
      atttatggc tgctcatta tgttttgtg aactctcac 
      ctcagcgcc cacgataat caactcaag gatcgcgtt 
      gaggattat aacaatatt attaaagat caagagttt 
      cacgttgaa gaaactacc gagatattt ttccatgat 
      tatgtattt tggatgggt gatctcaat tttaggctg 
      ctagaagag tacgatcag actcctgaa gagattgaa 
      agggatatt gtgaagaat gagctgaag aagcttttt 
      acttttgat cagttgaga tacgtaatg agaaaaggg 
      gaagctttt agtgaactt gaagaaaag gacccggaa 
      tttccacca actttcaaa tttgaagtt ggcaccaat 
      agatacgac ttcaagcgt cgacccgca tggtgcgac 
      cgaatacta tacagggtg aacccacac aactacgaa 
      aacgttacc ctcaaagtg gaccaatta tcctacaaa 
      catcatcct tcataccaa ctcagcgac cacaaaccg 
      gtcacagcc gaattcaac attaaggtc ttttcagat 
      tattctgaa cggattgtt gagttcgat aaaattgtt 
      agttggaat caagatgag gagaacaaa gcgagatat 
      aagatcacc aaggacatt ccggctact aaagacgac 
      tggattgga ttatttaaa gataacttc agtagtttg 
      gacgattac gtgagctat gagtatgtg agtaaatgc 
      gcatcgccg actcctgag aagaagagt ccaatgaaa 
      ggaggttcg tctaagaac gaacaaaaa tacgagatt 
      acattttcg gaattgccg aatcgatgt cgagggaat 
      tattgcttg gtttatttc agccagtct gaggataaa 
      gtcattagt gtgttgggg attagcaat agcttccct 
      atcgtgaag catgatagc gactag
    mRNA sequence
    • >TC002268 RA
      cttggaata gatttatct ctctctccc tccaggatt 
      tagaaatgt taaacgtca aaatcgatc taaatattt 
      atgccagta aatcagtac gccgtttct ctttaaatt 
      ttatctttt aaacgattt attgttgac acaatcagc 
      tatttctgc accacaacc cactacaca aaatggaaa 
      atttgcgat tatatgtgg gcacctaca atgtgggta 
      caagcaccc ccgatataa agctaaact cgttattag 
      ggttgcctg atgaccaca agacagata aattgcagc 
      taccagata tttacattt tgagttttc aagaagtga 
      aagcgcagc ctcaaaaca tgcttcttg atgcgctat 
      ttgacgacc cctggactt acgcgtgca aagaacttc 
      ttgacacct tcaactatg ttaaaatca agtcagtta 
      ggcttcagg gtttgttgc tcaatgttt attgtctca 
      ggaaacacc tactcaata ttagggaaa ttgagtctg 
      actacacca ggacagggc tgtcaggaa tgtggggta 
      ataaaggtg cagttagtg ttagactca gtatttatg 
      gctgctcat tatgttttg tgaactctc acctcagcg 
      cccacgata atcaactca aggatcgcg ttgaggatt 
      ataacaata ttattaaag atcaagagt ttcacgttg 
      aagaaacta ccgagatat ttttccatg attatgtat 
      tttggatgg gtgatctca attttaggc tgctagaag 
      agtacgatc agactcctg aagagattg aaagggata 
      ttgtgaaga atgagctga agaagcttt ttacttttg 
      atcagttga gatacgtaa tgagaaaag gggaagctt 
      ttagtgaac ttgaagaaa aggacccgg aatttccac 
      caactttca aatttgaag ttggcacca atagatacg 
      acttcaagc gtcgacccg catggtgcg accgaatac 
      tatacaggg tgaacccac acaactacg aaaacgtta 
      ccctcaaag tggaccaat tatcctaca aacatcatc 
      cttcatacc aactcagcg accacaaac cggtcacag 
      ccgaattca acattaagg tcttttcag attattctg 
      aacggattg ttgagttcg ataaaattg ttagttgga 
      atcaagatg aggagaaca aagcgagat ataagatca 
      ccaaggaca ttccggcta ctaaagacg actggattg 
      gattattta aagataact tcagtagtt tggacgatt 
      acgtgagct atgagtatg tgagtaaat gcgcatcgc 
      cgactcctg agaagaaga gtccaatga aaggaggtt 
      cgtctaaga acgaacaaa aatacgaga ttacatttt 
      cggaattgc cgaatcgat gtcgaggga attattgct 
      tggtttatt tcagccagt ctgaggata aagtcatta 
      gtgtgttgg ggattagca atagcttcc ctatcgtga 
      agcatgata gcgactaga aaatggttc catcacaat 
      tccgccctt tttaattaa ttgttattt agttttatt 
      ttcttactt tgaaattta cttttattg agtaattgt 
      gccttgtac aactttcca cttttatta tataaggga 
      gtatcctat tgttgattt tattgcagc gttgtgaac 
      aagttttaa agttttata ttttttatg ccagtgcta 
      gtcaaaaaa atccacttt ttcactcta cacaataca 
      atagatacc ttgcctaca atagtattg acaaaactg 
      agcctttat tatgtgata cgtatgtag aacttgctc 
      gactattgt tttactgaa ttacatttt atacataaa 
      atacgacat tagaaaatg aatgaaggt tgaaagttt 
      tgttttaac tcttgaaat ctattttta ttatatgtt 
      aacatattt ttttctagc ggaatgtgg tcagaagta 
      ctatgaaaa ttcaatgca cgtatattt ttcctaaat 
      tgttatgat taatcaaat gacaaatta tttatgcta 
      ta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY