| General information | |||
| TC002264 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NW_015451937.1: 529072..616685 | |
| Sequence information | |||
| Transcripts / Proteins (3) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC002264 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_03337 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC002264 RA
MSCVWSNSG LKRAGKISE CGKYCGPTA NGPTISFSS FRQSFTSNV NYLKRRFSS GDLSSECED GEAPVDPYS KAPPVTAQP QAPPPPPPS QTSTVGDLS LNLRPGSKT TSAPSSPAK SRESLLQRV QSLTGQARD QGASILGAA VSSATRVTS SVSGRYLTL LVVDDQNTD WSKYFRGKR IGDYDIRVE QAEFKEITV TANSEGANV SMAVFRGGT RVGRSFRPD FLLVRQNLK DAGENYKKL LLALKFGGV PSINNLNSI YNFQDKPWV FGHLVQLQR RLGKDSFPL IEQTFYPNH REMITAPRF PVVLKIGHA HGGLGKVKV DNINDFQDM ASVVAVANT YCTVEPYID SKYDIHVQK IGNNYKAFM RKSISGCWK TNTGSAMLE QISMPERYR SWIDEVSDL FGGLDICAL EVVVGKDGR EFIIEVNDS ALTLLGDSQ EEDRRHIAD LVASRMQAI CRPRTPPSE EPVPPPVGP RGILGSLTS LTHSETPPP SASDHPSLS SVGRRDSQA SQSSTVSSS GPSTGRLPD PPPRPFQRQ GSQQQSTTE DTEDTMKNL RKTFAGIFG DM
- >TC002264 RB
MCKIALVIT APRFPVVLK IGHAHGGLG KVKVDNIND FQDMASVVA VANTYCTVE PYIDSKYDI HVQKIGNNY KAFMRKSIS GCWKTNTGS AMLEQISMP ERYRSWIDE VSDLFGGLD ICALEVVVG KDGREFIIE VNDSALTLL GDSQEEDRR HIADLVASR MQAICRPRT PPSEEPVPP PVGPRGILG SLTSLTHSE TPPPSASDH PSLSSVGRR DSQASQSST VSSSGPSTG RLPDPPPRP FQRQGSQQQ STTEDTEDT MKNLRKTFA GIFGDM
- >TC002264 RC
MRRAGKISE CGKYCGPTA NGPTISFSS FRQSFTSNV NYLKRRFSS GDLSSECED GEAPVDPYS KAPPVTAQP QAPPPPPPS QTSTVGDLS LNLRPGSKT TSAPSSPAK SRESLLQRV QSLTGQARD QGASILGAA VSSATRVTS SVSGRYLTL LVVDDQNTD WSKYFRGKR IGDYDIRVE QAEFKEITV TANSEGANV SMAVFRGGT RVGRSFRPD FLLVRQNLK DAGENYKKL LLALKFGGV PSINNLNSI YNFQDKPWV FGHLVQLQR RLGKDSFPL IEQTFYPNH REMLRLDTH QELDNYVTN VFEML
- >TC002264 RA
atgagttgc gtttggagc aattcaggc ctgaaaaga gccggcaaa atttccgag tgcggcaaa tactgcggc cccaccgca aacggcccc accatttcg ttcagcagc tttagacag tccttcacc tctaatgtc aattacctg aaaagacgt ttcagctcc ggggattta tcctcggag tgtgaagat ggcgaagcg ccggttgat ccgtattcg aaggcgccc cccgtcact gcgcagcca caggctcct ccaccaccg cccccatct cagacatcg accgtcggt gatttgtcg ctcaatctt cgaccaggc tccaagacg acgagtgcc ccaagcagt cctgccaag tcccgcgag tctctccta caaagagtg cagtctttg acaggacag gctcgagat caaggggct tcgatttta ggagctgca gtctctagt gcgactaga gtgacatcc tcggtttct ggaagatat ctcactttg ctcgttgtc gatgaccag aacacggac tggtcgaag tatttccgg gggaaaagg attggggat tatgacatt agggtggag caagccgag tttaaggaa attacggtc acggccaat tcggagggg gctaatgtg tcaatggcg gtgtttaga ggcgggaca agagttggg aggtcattc cgaccagat ttcttgctg gttcgacaa aacttgaag gacgcaggc gaaaactac aagaaactg ttactggca ctaaaattt ggaggcgtc ccaagcatc aataattta aactcaatt tacaacttc caggacaaa ccgtgggtt tttggccat ttagtccaa ctccagaga agacttgga aaagacagc ttcccactc atcgaacag accttctat ccgaaccac cgagaaatg ataaccgct cctcgcttt cccgtcgta ttaaaaatc gggcacgcc catggcggt ctgggcaag gtgaaagtg gacaacatc aacgatttc caagacatg gcgagcgtc gtcgccgtc gcgaacacc tactgcact gttgaacct tacatcgac tccaaatac gacatccat gtccagaaa atcggcaac aactacaaa gccttcatg cgcaaatcc atctccgga tgttggaag accaacacg ggctcagct atgctggaa cagatctcg atgccggaa cgctaccgt tcttggatt gatgaagtc tctgatttg tttggagga ttggatatc tgtgctttg gaggtcgtg gttgggaaa gacgggaga gagttcata atcgaagtg aatgacagc gcactgact cttctgggg gattcacaa gaggaggat cggagacat attgccgat ttggtggcg tcaaggatg caggctatt tgtagaccg aggactccg ccaagtgag gaaccggta cctccccca gttgggcca agagggatt ttgggcagc ttgaccagc ttgacacat tcagagact ccgccacct agtgcttct gatcatcct tcgttgtcg agtgtgggc agacgagac agccaagca tctcagagc agtacagtt agcagcagc ggtcccagc actggccgt cttccagac cctccacct cgcccattc caacgccaa ggcagtcaa cagcaatca acaactgaa gacaccgaa gacacaatg aagaactta cgaaaaaca ttcgctggt atatttggc gacatgtaa
- >TC002264 RB
atgtgcaaa attgcactt gtcataacc gctcctcgc tttcccgtc gtattaaaa atcgggcac gcccatggc ggtctgggc aaggtgaaa gtggacaac atcaacgat ttccaagac atggcgagc gtcgtcgcc gtcgcgaac acctactgc actgttgaa ccttacatc gactccaaa tacgacatc catgtccag aaaatcggc aacaactac aaagccttc atgcgcaaa tccatctcc ggatgttgg aagaccaac acgggctca gctatgctg gaacagatc tcgatgccg gaacgctac cgttcttgg attgatgaa gtctctgat ttgtttgga ggattggat atctgtgct ttggaggtc gtggttggg aaagacggg agagagttc ataatcgaa gtgaatgac agcgcactg actcttctg ggggattca caagaggag gatcggaga catattgcc gatttggtg gcgtcaagg atgcaggct atttgtaga ccgaggact ccgccaagt gaggaaccg gtacctccc ccagttggg ccaagaggg attttgggc agcttgacc agcttgaca cattcagag actccgcca cctagtgct tctgatcat ccttcgttg tcgagtgtg ggcagacga gacagccaa gcatctcag agcagtaca gttagcagc agcggtccc agcactggc cgtcttcca gaccctcca cctcgccca ttccaacgc caaggcagt caacagcaa tcaacaact gaagacacc gaagacaca atgaagaac ttacgaaaa acattcgct ggtatattt ggcgacatg taa
- >TC002264 RC
atgagaaga gccggcaaa atttccgag tgcggcaaa tactgcggc cccaccgca aacggcccc accatttcg ttcagcagc tttagacag tccttcacc tctaatgtc aattacctg aaaagacgt ttcagctcc ggggattta tcctcggag tgtgaagat ggcgaagcg ccggttgat ccgtattcg aaggcgccc cccgtcact gcgcagcca caggctcct ccaccaccg cccccatct cagacatcg accgtcggt gatttgtcg ctcaatctt cgaccaggc tccaagacg acgagtgcc ccaagcagt cctgccaag tcccgcgag tctctccta caaagagtg cagtctttg acaggacag gctcgagat caaggggct tcgatttta ggagctgca gtctctagt gcgactaga gtgacatcc tcggtttct ggaagatat ctcactttg ctcgttgtc gatgaccag aacacggac tggtcgaag tatttccgg gggaaaagg attggggat tatgacatt agggtggag caagccgag tttaaggaa attacggtc acggccaat tcggagggg gctaatgtg tcaatggcg gtgtttaga ggcgggaca agagttggg aggtcattc cgaccagat ttcttgctg gttcgacaa aacttgaag gacgcaggc gaaaactac aagaaactg ttactggca ctaaaattt ggaggcgtc ccaagcatc aataattta aactcaatt tacaacttc caggacaaa ccgtgggtt tttggccat ttagtccaa ctccagaga agacttgga aaagacagc ttcccactc atcgaacag accttctat ccgaaccac cgagaaatg cttcggtta gacactcac caagaattg gacaactac gtaacaaat gtctttgag atgctctag
- >TC002264 RA
tttgctgac acttgtttg gaaatttag ttagatttt cttcaaatc tgttcaaaa ataccgaca gaaactcaa aaaagggca ccttgccca aagtcccgt ttgagcgtt cattacgca aaacgtaaa tttttggtg ctatcgcga ttaaactcc ggatgtccg gaaaaaatt gcgcacatt tttgagcat gtgtgactg agagagaaa gtcatgatt tatttaaat caaacaaaa tacgcacct ccggaaccc atcctatcg ccaaaaacg caaaaatcc ggaacaacc actcggaac aaaatctac ttttgacac ccatttcga cgtaaacaa acaatcaca tgttcaaaa acggccact gttttacta caacacact cgtttgcac cattctctt gtgttaatt taactggaa tgaaagtta agagttgct cgtctgtgc tagcgttgg gatctccgg aaagtagtt tcgatacgg tgcaaacga ccatgcttt accaccaca atagcaaaa ttcatttcc gggcggaac atattcacc tctacgacg aaccattga ctgaaaaat tggaattaa agagggaaa aacgatttt tcaagtttt gctgaaatt ttagaatta aacgggcgc cgaaaataa ctgctcctg aagccgtta gaggcagtg ctgtgtcta tcgaccgca aacttgtcg atttcagcg ccactacag ccgccctct aaaagtccg cacgcattt cagtcggtt tcagaagct ccgttcagt tactgaaaa tcgccgatt tcttcacga actgtacgt ataccttgt taagcgcct taagtgtag attatttta cgcacagct aagtgaaat gagttgcgt ttggagcaa ttcaggcct gaaaagagc cggcaaaat ttccgagtg cggcaaata ctgcggccc caccgcaaa cggccccac catttcgtt cagcagctt tagacagtc cttcacctc taatgtcaa ttacctgaa aagacgttt cagctccgg ggatttatc ctcggagtg tgaagatgg cgaagcgcc ggttgatcc gtattcgaa ggcgccccc cgtcactgc gcagccaca ggctcctcc accaccgcc cccatctca gacatcgac cgtcggtga tttgtcgct caatcttcg accaggctc caagacgac gagtgcccc aagcagtcc tgccaagtc ccgcgagtc tctcctaca aagagtgca gtctttgac aggacaggc tcgagatca aggggcttc gattttagg agctgcagt ctctagtgc gactagagt gacatcctc ggtttctgg aagatatct cactttgct cgttgtcga tgaccagaa cacggactg gtcgaagta tttccgggg gaaaaggat tggggatta tgacattag ggtggagca agccgagtt taaggaaat tacggtcac ggccaattc ggagggggc taatgtgtc aatggcggt gtttagagg cgggacaag agttgggag gtcattccg accagattt cttgctggt tcgacaaaa cttgaagga cgcaggcga aaactacaa gaaactgtt actggcact aaaatttgg aggcgtccc aagcatcaa taatttaaa ctcaattta caacttcca ggacaaacc gtgggtttt tggccattt agtccaact ccagagaag acttggaaa agacagctt cccactcat cgaacagac cttctatcc gaaccaccg agaaatgat aaccgctcc tcgctttcc cgtcgtatt aaaaatcgg gcacgccca tggcggtct gggcaaggt gaaagtgga caacatcaa cgatttcca agacatggc gagcgtcgt cgccgtcgc gaacaccta ctgcactgt tgaacctta catcgactc caaatacga catccatgt ccagaaaat cggcaacaa ctacaaagc cttcatgcg caaatccat ctccggatg ttggaagac caacacggg ctcagctat gctggaaca gatctcgat gccggaacg ctaccgttc ttggattga tgaagtctc tgatttgtt tggaggatt ggatatctg tgctttgga ggtcgtggt tgggaaaga cgggagaga gttcataat cgaagtgaa tgacagcgc actgactct tctggggga ttcacaaga ggaggatcg gagacatat tgccgattt ggtggcgtc aaggatgca ggctatttg tagaccgag gactccgcc aagtgagga accggtacc tcccccagt tgggccaag agggatttt gggcagctt gaccagctt gacacattc agagactcc gccacctag tgcttctga tcatccttc gttgtcgag tgtgggcag acgagacag ccaagcatc tcagagcag tacagttag cagcagcgg tcccagcac tggccgtct tccagaccc tccacctcg cccattcca acgccaagg cagtcaaca gcaatcaac aactgaaga caccgaaga cacaatgaa gaacttacg aaaaacatt cgctggtat atttggcga catgtaaac gctctgact agagaccga ctgtttgct tttgaatcg tcaaaagca gacaggtca aagcaatat acctgcata aaaggtgtg ttactccac tgcctaggg ccaggtact tgtgggaag tacctttgt tatgtttag cctagttgt taattggat gtataacag tgtactcgt tattagacg ttttgttat gggatagag tgagacatt aaggggcta agcgaacac atcctcttt ccctcttct gtagtagta cttttacca attgtaaaa tgacaatat tattgtaat cctgctcct gcactcctc tctgtaacc gtttcgtca acttattgt tagattgtg ggttgttca tcgattgtt agagcaacg aatgtttgt aataacgtt tccagagaa ttgttattc gccatagtc cgaaaattc tacaaacgc acactggtt caaaacccc aactagcag aagtgcgca actttacct cctggtgcg aatccgtac aatttttag aagacacgt tgtgccttt gtagaattt ttagtttat agcagcgat tctctcggg tttatcctc tttggtccg gaacagtga agttgcgca cactggacc tgatcgaac cgaataagc ctcaagagt atgacatta tgtaataat tagatttta catgatctg tagattaga tttacgtag tgcgttgtc tcctttatc acatttgtt aggccttgg cgagttttt tacaaagtt atctgtagc tggctacga tgttttcaa atgagtgaa tataatatt attaacata ttaggcaaa agtattcaa tttgcattt aatatattg taagcatat catgtttgt ttgtgagat tcgctcagt gttgacttg catatccat ttaagtgtg tgcgttttt cgttttgga gacagcaac tcgaggttg tgtagtagc tcagcttac aaataatac gttcgggca tattgtata gtgttatat cgatggaaa tacaataaa tgagtaaat aagtg
- >TC002264 RB
gatagaaaa ccagtagga atgtgcaaa attgcactt gtcataacc gctcctcgc tttcccgtc gtattaaaa atcgggcac gcccatggc ggtctgggc aaggtgaaa gtggacaac atcaacgat ttccaagac atggcgagc gtcgtcgcc gtcgcgaac acctactgc actgttgaa ccttacatc gactccaaa tacgacatc catgtccag aaaatcggc aacaactac aaagccttc atgcgcaaa tccatctcc ggatgttgg aagaccaac acgggctca gctatgctg gaacagatc tcgatgccg gaacgctac cgttcttgg attgatgaa gtctctgat ttgtttgga ggattggat atctgtgct ttggaggtc gtggttggg aaagacggg agagagttc ataatcgaa gtgaatgac agcgcactg actcttctg ggggattca caagaggag gatcggaga catattgcc gatttggtg gcgtcaagg atgcaggct atttgtaga ccgaggact ccgccaagt gaggaaccg gtacctccc ccagttggg ccaagaggg attttgggc agcttgacc agcttgaca cattcagag actccgcca cctagtgct tctgatcat ccttcgttg tcgagtgtg ggcagacga gacagccaa gcatctcag agcagtaca gttagcagc agcggtccc agcactggc cgtcttcca gaccctcca cctcgccca ttccaacgc caaggcagt caacagcaa tcaacaact gaagacacc gaagacaca atgaagaac ttacgaaaa acattcgct ggtatattt ggcgacatg taaacgctc tgactagag accgactgt ttgcttttg aatcgtcaa aagcagaca ggtcaaagc aatatacct gcataaaag gtgtgttac tccactgcc tagggccag gtacttgtg ggaagtacc tttgttatg tttagccta gttgttaat tggatgtat aacagtgta ctcgttatt agacgtttt gttatggga tagagtgag acattaagg ggctaagcg aacacatcc tctttccct cttctgtag tagtacttt taccaattg taaaatgac aatattatt gtaatcctg ctcctgcac tcctctctg taaccgttt cgtcaactt attgttaga ttgtgggtt gttcatcga ttgttagag caacgaatg tttgtaata acgtttcca gagaattgt tattcgcca tagtccgaa aattctaca aacgcacac tggttcaaa accccaact agcagaagt gcgcaactt tacctcctg gtgcgaatc cgtacaatt tttagaaga cacgttgtg cctttgtag aatttttag tttatagca gcgattctc tcgggttta tcctctttg gtccggaac agtgaagtt gcgcacact ggacctgat cgaaccgaa taagcctca agagtatga cattatgta ataattaga ttttacatg atctgtaga ttagattta cgtagtgcg ttgtctcct ttatcacat ttgttaggc cttggcgag ttttttaca aagttatct gtagctggc tacgatgtt ttcaaatga gtgaatata atattatta acatattag gcaaaagta ttcaatttg catttaata tattgtaag catatcatg tttgtttgt gagattcgc tcagtgttg acttgcata tccatttaa gtgtgtgcg tttttcgtt ttggagaca gcaactcga ggttgtgta gtagctcag cttacaaat aatacgttc gggcatatt gtatagtgt tatatcgat ggaaataca ataaatgag taaataagt g
- >TC002264 RC
ctacaatct cgtgcaatg agaagagcc ggcaaaatt tccgagtgc ggcaaatac tgcggcccc accgcaaac ggccccacc atttcgttc agcagcttt agacagtcc ttcacctct aatgtcaat tacctgaaa agacgtttc agctccggg gatttatcc tcggagtgt gaagatggc gaagcgccg gttgatccg tattcgaag gcgcccccc gtcactgcg cagccacag gctcctcca ccaccgccc ccatctcag acatcgacc gtcggtgat ttgtcgctc aatcttcga ccaggctcc aagacgacg agtgcccca agcagtcct gccaagtcc cgcgagtct ctcctacaa agagtgcag tctttgaca ggacaggct cgagatcaa ggggcttcg attttagga gctgcagtc tctagtgcg actagagtg acatcctcg gtttctgga agatatctc actttgctc gttgtcgat gaccagaac acggactgg tcgaagtat ttccggggg aaaaggatt ggggattat gacattagg gtggagcaa gccgagttt aaggaaatt acggtcacg gccaattcg gagggggct aatgtgtca atggcggtg tttagaggc gggacaaga gttgggagg tcattccga ccagatttc ttgctggtt cgacaaaac ttgaaggac gcaggcgaa aactacaag aaactgtta ctggcacta aaatttgga ggcgtccca agcatcaat aatttaaac tcaatttac aacttccag gacaaaccg tgggttttt ggccattta gtccaactc cagagaaga cttggaaaa gacagcttc ccactcatc gaacagacc ttctatccg aaccaccga gaaatgctt cggttagac actcaccaa gaattggac aactacgta acaaatgtc tttgagatg ctctagtta ctccttttc ttgtaacaa tatgcttta agaaagctg attccaatt t
