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Gene information
General information
TC002259 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid1809: 785811..791610
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002259
    iBeetle screen iB_03334
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    vitellogenic egg chamber late mostlynot present - Number of animals showing the phenotype: 4

    vitellogenic egg chamber late sometimesfused - Number of animals showing the phenotype: 2
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002259 RA
      MDDGTPKRG RGRGRGGRG RGRGRGRGR GRGKKAIKV 
      IESDEELDN SQLQESSEE AQQTESESN ENVAPKIDL 
      EADISQLEA PTFTTINRG PPEPMLRLD WSHKVNLIG 
      EKVLNPMIH CCDKCLKPI LIYGRMIPC KHVFCLSCG 
      KQEQKQCPR CLEKVSRVE QTGLGTVFM CTHGGTRYG 
      SSGCRRTYL SHRDLQAHI NHRHVSNTI QPMEVVPVE 
      PERVSVRAK TNDPRSNIS ISSNVRQRP TPIQSSGVR 
      TNLITVPIQ DSAPTVHET SATQHSYYN YQQTATYNQ 
      GLPPMHIPP PQQPQYYAA PPAYSPAQS YSYSGGAPA 
      AGQYGGQGA QGRAAQYDY STAPPPQWT TGSQQYYR
    CDS sequence
    • >TC002259 RA
      atggacgat ggtaccccc aagaggggc cgcggccgg 
      ggtcgcggt ggtcgaggc agaggtcgg ggaagaggc 
      cgaggtcgc ggacgtggt aaaaaggcc ataaaagtg 
      atagaaagc gatgaagaa ctggataat tcccaactg 
      caagaatcg tccgaagag gcacaacaa actgaaagt 
      gaatctaac gaaaatgtg gcacctaaa attgatttg 
      gaagctgac atctcccaa ctggaagca ccgactttt 
      acaacaatt aacagaggg ccaccagaa ccaatgttg 
      cggctagat tggtcacat aaagtgaat ttgattggc 
      gaaaaagtg ctcaatccc atgattcat tgctgtgat 
      aagtgtttg aagccgatt ttaatctat ggacgaatg 
      attccgtgc aagcacgtc ttctgtctg tcatgtggc 
      aaacaagag cagaagcaa tgcccgcgc tgtttggag 
      aaagtatcc cgagtggag caaacaggt ttagggaca 
      gttttcatg tgtactcac ggagggact cgttacggt 
      tcctccggc tgccgccgt acttactta tcacaccgt 
      gatctccaa gcccacatc aaccaccgc cacgtgtcc 
      aacaccatc cagccgatg gaagttgtt cccgtcgaa 
      ccagaacga gtgtcagtc cgcgctaaa acgaacgat 
      cccagatcg aacatttca atatcatca aatgtgaga 
      cagcgccca actccgata caaagttcc ggagttcgg 
      actaatttg atcacggtg ccaattcag gattcggca 
      ccgacagtg cacgagact agcgctacg caacatagt 
      tattacaat taccaacag actgcgacg tataatcag 
      ggcttgcct ccaatgcat attccgcca cctcagcag 
      ccacagtat tatgctgcg cctccggct tatagtccg 
      gctcagagt tatagttat agtggcgga gcgccggct 
      gcgggacag tatgggggc cagggggcg caagggagg 
      gccgcgcag tatgactac agcacggcg cctccgcca 
      caatggacc accggtagc cagcaatac tacagatga
    mRNA sequence
    • >TC002259 RA
      ggattgata attttaatg attaagtta atttattgg 
      ggtttgtat tcaattttg aatggtgta ccaagtgga 
      ttggtatcc cttcatttg acaaagtct taacctcaa 
      aattatgga cgctcttac gtcaacgat caagtaaat 
      aaaagatgg acgatggta cccccaaga ggggccgcg 
      gccggggtc gcggtggtc gaggcagag gtcggggaa 
      gaggccgag gtcgcggac gtggtaaaa aggccataa 
      aagtgatag aaagcgatg aagaactgg ataattccc 
      aactgcaag aatcgtccg aagaggcac aacaaactg 
      aaagtgaat ctaacgaaa atgtggcac ctaaaattg 
      atttggaag ctgacatct cccaactgg aagcaccga 
      cttttacaa caattaaca gagggccac cagaaccaa 
      tgttgcggc tagattggt cacataaag tgaatttga 
      ttggcgaaa aagtgctca atcccatga ttcattgct 
      gtgataagt gtttgaagc cgattttaa tctatggac 
      gaatgattc cgtgcaagc acgtcttct gtctgtcat 
      gtggcaaac aagagcaga agcaatgcc cgcgctgtt 
      tggagaaag tatcccgag tggagcaaa caggtttag 
      ggacagttt tcatgtgta ctcacggag ggactcgtt 
      acggttcct ccggctgcc gccgtactt acttatcac 
      accgtgatc tccaagccc acatcaacc accgccacg 
      tgtccaaca ccatccagc cgatggaag ttgttcccg 
      tcgaaccag aacgagtgt cagtccgcg ctaaaacga 
      acgatccca gatcgaaca tttcaatat catcaaatg 
      tgagacagc gcccaactc cgatacaaa gttccggag 
      ttcggacta atttgatca cggtgccaa ttcaggatt 
      cggcaccga cagtgcacg agactagcg ctacgcaac 
      atagttatt acaattacc aacagactg cgacgtata 
      atcagggct tgcctccaa tgcatattc cgccacctc 
      agcagccac agtattatg ctgcgcctc cggcttata 
      gtccggctc agagttata gttatagtg gcggagcgc 
      cggctgcgg gacagtatg ggggccagg gggcgcaag 
      ggagggccg cgcagtatg actacagca cggcgcctc 
      cgccacaat ggaccaccg gtagccagc aatactaca 
      gatgaatat tttaagtag aatctattt atgtctaag 
      actaatttt ctagattta tctatgtct acatttaag 
      ttttgtaag ctagtgact atcggttta ataaatgtt 
      ttcaatact g