Status
Please wait ...
Gene information
General information
TC002258 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451937.1: 796859..810004
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002258
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03333

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002258 RA
      MSEKERNCS NEAVEKIIG NMTAEMQNA DGQYAPLGF 
      TPPPSEPLN WTQPPYVNN YSYNPASGY VPMQQFATP 
      FNYMQQPHA FDMNEYNKN LKYMYEPLQ NGGDGGVFR 
      NEFILNANL HQSEPLPQT SNHAHLIEN LVGNWVPNT 
      TGTYSPFGN VEPFKTFEQ ENEQNLILE QPLEEKQFN 
      FSRTNRKPR MVAEVKPMR PSYSDVLTK AVPPNTPKP 
      TVTKDPKVK KESGKKNGK IEKTQKVNC SLNRSNTNA 
      DIKDIPISE KTQFIKTSD KDKRPRSTQ LNRKWASLD 
      NINEPINNI KVDDGKKKK DEVTNSTKN LSQKSNFRK 
      VTKNPGDSP ENETFVGKN NETLYVSKN GLKKTSKFN 
      NSRTKNLEA NERPPGKRN QRTRKRENQ VFFGIAGQK 
      LKHYLKGWW KILTVFFFW LIHLISDIC SLSVHISRD 
      MGSNFWLWL CVHWNLFLD TVVTIASRI RILSWIWEK 
      FKKPEKPTT KDNNRSFIH SGLQNNINM PTTGEEAMK 
      RLLACKGKD PYSILGVTP TCTDDDIKR YYKRQAFLV 
      HPDKNQQPG AEEAFKILV HAFDMIGEP ERRAAYDKG 
      VVESVQVEQ AWSELTELL AQLQQKVEA AANTIRCSA 
      CGLRHKRIK VDRPCYAAR NCSSCKIHH SAREGDIWA 
      EARCFGFLW HYYACMEGS VYDITEWAG CQKDSLKHL 
      RPDSHQVQY RIALGKQNS QPRRHSGSQ RNEKPPDLE 
      NLLNTLYGQ TDCMQQGQK RRSKKGK
    CDS sequence
    • >TC002258 RA
      atgagtgaa aaggagagg aactgttca aacgaagcg 
      gtggaaaaa atcataggc aacatgaca gcggaaatg 
      caaaatgca gatggtcag tatgccccc ttgggtttc 
      acgccgccc ccgagcgag cccctgaac tggacccag 
      cccccttac gtgaacaac tactcgtac aaccctgcc 
      tcaggctat gtacctatg caacaattt gcaaccccc 
      ttcaattac atgcaacag ccccacgcg ttcgacatg 
      aacgaatac aataagaat ttgaaatac atgtacgag 
      ccgttgcaa aatgggggc gatggtggc gtttttcga 
      aacgaattt atcctaaat gcgaatttg caccaatcg 
      gagcctttg ccccagacc agcaaccat gcccacttg 
      atcgagaat ttggtgggc aattgggtg cccaacact 
      acagggacg tactcgccc tttggcaac gtcgagccg 
      tttaagact ttcgaacaa gaaaatgaa caaaactta 
      atacttgaa caacccctt gaagagaaa cagtttaat 
      ttttctcga accaatcgc aagccaaga atggtggca 
      gaggtgaaa ccaatgcgc cccagctac tctgatgtt 
      ctgacaaag gctgtgccc ccaaatacg cccaaacca 
      accgtcact aaagacccc aaagtgaag aaagaaagt 
      ggcaaaaag aacggcaaa attgagaaa acgcaaaaa 
      gtgaattgt tctttgaac cgaagcaac acgaacgcg 
      gacattaaa gatattcca atttcggag aaaacccaa 
      tttatcaaa accagcgat aaagataag cgtcccaga 
      agcacgcaa ctgaaccgc aagtgggcc tcgcttgat 
      aatatcaac gaacccatc aacaatatc aaggttgat 
      gatggtaaa aagaagaag gatgaggtt accaacagt 
      acgaaaaat ttgagccaa aaatcgaat tttcgaaaa 
      gttaccaaa aatccgggg gattctccg gagaatgaa 
      acttttgtg ggtaagaat aacgagacg ctttatgtt 
      agcaaaaat gggctgaag aagacgagc aagtttaat 
      aattcgagg accaaaaat ttggaagct aatgagagg 
      ccgccaggg aagcgaaac caacggact cgcaagaga 
      gaaaatcaa gtttttttt ggaattgct ggccaaaaa 
      ttgaaacat tacttgaaa ggatggtgg aagatttta 
      accgttttc ttcttctgg ctcattcac cttatctcc 
      gatatttgc agcctgagc gttcacatt agccgtgac 
      atgggttcg aatttttgg ctttggttg tgtgtccat 
      tggaacctc tttttggat acggtagtg accatcgca 
      tcacgtatt cgaatatta tcgtggatt tgggagaaa 
      ttcaagaaa cctgaaaaa ccaacaact aaagacaat 
      aatcgcagt tttattcac agtggatta cagaataat 
      attaatatg ccaacgact ggcgaagaa gcaatgaaa 
      agactttta gcatgtaaa ggcaaggac ccttacagt 
      atattggga gttactcca acttgtact gacgatgat 
      attaagagg tactataaa agacaagct tttttggtc 
      catcctgac aagaaccaa caaccggga gccgaagaa 
      gcctttaaa atattagtt catgccttc gatatgatc 
      ggggaaccg gaaagaagg gccgcttac gataaaggt 
      gttgtggag tccgtgcaa gtggagcag gcctggagt 
      gaattaacg gagcttttg gcccagctg cagcaaaag 
      gttgaagca gccgctaat acaattcgg tgcagtgct 
      tgcggttta cgtcacaaa agaatcaaa gtggataga 
      ccttgttac gcggcaagg aactgttcc tcttgtaaa 
      atccatcat tcggctcga gaaggtgac atttgggca 
      gaagctcgc tgtttcggc tttttgtgg cattactac 
      gcttgtatg gaaggcagt gtgtacgat ataacggaa 
      tgggccggt tgtcagaag gacagtctc aaacaccta 
      agaccagac tcgcatcaa gtacaatat cgcatcgct 
      ttaggtaaa caaaatagt caacctcgt agacacagc 
      ggatctcag cggaatgag aaaccaccc gatctcgaa 
      aatttactt aacacattg tacggacaa accgattgc 
      atgcaacaa ggacagaaa cgtaggagt aaaaaagga 
      aagtga
    mRNA sequence
    • >TC002258 RA
      cgtcaaaag tgacagcgt tgaataata tgtttatgt 
      gccaaaatg ttacaaaaa tagccgtac actagtaaa 
      tttggtcgt tgttttgtg aaaaactgt gcaaaatga 
      gtgaaaagg agaggaact gttcaaacg aagcggtgg 
      aaaaaatca taggcaaca tgacagcgg aaatgcaaa 
      atgcagatg gtcagtatg cccccttgg gtttcacgc 
      cgcccccga gcgagcccc tgaactgga cccagcccc 
      cttacgtga acaactact cgtacaacc ctgcctcag 
      gctatgtac ctatgcaac aatttgcaa cccccttca 
      attacatgc aacagcccc acgcgttcg acatgaacg 
      aatacaata agaatttga aatacatgt acgagccgt 
      tgcaaaatg ggggcgatg gtggcgttt ttcgaaacg 
      aatttatcc taaatgcga atttgcacc aatcggagc 
      ctttgcccc agaccagca accatgccc acttgatcg 
      agaatttgg tgggcaatt gggtgccca acactacag 
      ggacgtact cgccctttg gcaacgtcg agccgttta 
      agactttcg aacaagaaa atgaacaaa acttaatac 
      ttgaacaac cccttgaag agaaacagt ttaattttt 
      ctcgaacca atcgcaagc caagaatgg tggcagagg 
      tgaaaccaa tgcgcccca gctactctg atgttctga 
      caaaggctg tgcccccaa atacgccca aaccaaccg 
      tcactaaag accccaaag tgaagaaag aaagtggca 
      aaaagaacg gcaaaattg agaaaacgc aaaaagtga 
      attgttctt tgaaccgaa gcaacacga acgcggaca 
      ttaaagata ttccaattt cggagaaaa cccaattta 
      tcaaaacca gcgataaag ataagcgtc ccagaagca 
      cgcaactga accgcaagt gggcctcgc ttgataata 
      tcaacgaac ccatcaaca atatcaagg ttgatgatg 
      gtaaaaaga agaaggatg aggttacca acagtacga 
      aaaatttga gccaaaaat cgaattttc gaaaagtta 
      ccaaaaatc cgggggatt ctccggaga atgaaactt 
      ttgtgggta agaataacg agacgcttt atgttagca 
      aaaatgggc tgaagaaga cgagcaagt ttaataatt 
      cgaggacca aaaatttgg aagctaatg agaggccgc 
      cagggaagc gaaaccaac ggactcgca agagagaaa 
      atcaagttt tttttggaa ttgctggcc aaaaattga 
      aacattact tgaaaggat ggtggaaga ttttaaccg 
      ttttcttct tctggctca ttcacctta tctccgata 
      tttgcagcc tgagcgttc acattagcc gtgacatgg 
      gttcgaatt tttggcttt ggttgtgtg tccattgga 
      acctctttt tggatacgg tagtgacca tcgcatcac 
      gtattcgaa tattatcgt ggatttggg agaaattca 
      agaaacctg aaaaaccaa caactaaag acaataatc 
      gcagtttta ttcacagtg gattacaga ataatatta 
      atatgccaa cgactggcg aagaagcaa tgaaaagac 
      ttttagcat gtaaaggca aggaccctt acagtatat 
      tgggagtta ctccaactt gtactgacg atgatatta 
      agaggtact ataaaagac aagcttttt tggtccatc 
      ctgacaaga accaacaac cgggagccg aagaagcct 
      ttaaaatat tagttcatg ccttcgata tgatcgggg 
      aaccggaaa gaagggccg cttacgata aaggtgttg 
      tggagtccg tgcaagtgg agcaggcct ggagtgaat 
      taacggagc ttttggccc agctgcagc aaaaggttg 
      aagcagccg ctaatacaa ttcggtgca gtgcttgcg 
      gtttacgtc acaaaagaa tcaaagtgg atagacctt 
      gttacgcgg caaggaact gttcctctt gtaaaatcc 
      atcattcgg ctcgagaag gtgacattt gggcagaag 
      ctcgctgtt tcggctttt tgtggcatt actacgctt 
      gtatggaag gcagtgtgt acgatataa cggaatggg 
      ccggttgtc agaaggaca gtctcaaac acctaagac 
      cagactcgc atcaagtac aatatcgca tcgctttag 
      gtaaacaaa atagtcaac ctcgtagac acagcggat 
      ctcagcgga atgagaaac cacccgatc tcgaaaatt 
      tacttaaca cattgtacg gacaaaccg attgcatgc 
      aacaaggac agaaacgta ggagtaaaa aaggaaagt 
      gatttggca ctagatgca taagttgtg tattttatt 
      tcgctatga gtatgtgct atcaattta taaaatcaa 
      tgcgcttga aaaataaat catgtttac aaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY