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Gene information
General information
TC002246 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid36: 604267..608191
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002246
    iBeetle screen iB_03331
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    Fertility
    egg chamber number decreased - Number of animals showing the phenotype: 4

    vitellogenic egg chamber late mostlynot present - Number of animals showing the phenotype: 4
    vitellogenic egg chamber early mostlynot present - Number of animals showing the phenotype: 4
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002246 RA
      MSTKESMEM EVDNPASAD VANNPAEDS EKSVMAAGA 
      VGSVTCSLH PLVIMNVSE HWTREKAQE GSVQRVIGA 
      LIGKQKGRN IEIMNSFEL VFSVIGGDV IIDRDYYNM 
      KEEQFKQVF SDMDFIGWY TTGDIPNTT DIKIHKQIC 
      DINESPILL KLNPYDKNI DHLPVALYE SVIDLIGGE 
      ATMLFVNLS YTLATEEAE RIGVDHVAR MSSTDSGES 
      SLVAEHLTA QHSAIKMLH SRVRLVLEY MKAVQSGQL 
      PTNHEILRE AYSLCHRLP VIQSARFRQ DFYNQCNDV 
      GLMTYLGTL TKGCNDLNQ FVNKFNILY DRQGLGRRM 
      RGIFF
    CDS sequence
    • >TC002246 RA
      atgagtacg aaagaatca atggaaatg gaagtcgat 
      aatccggct tctgctgat gttgcaaat aatccggcg 
      gaagattcg gaaaaaagt gtgatggca gctggggct 
      gtcggttct gtgacttgt tcattgcac cctttggtg 
      ataatgaat gtttctgag cactggaca cgtgaaaag 
      gctcaagag ggctcagtc caacgagtg attggggct 
      ttgattggt aaacagaag gggcgaaac attgaaata 
      atgaactca tttgaactg gtatttagt gtaattgga 
      ggggatgtt attatcgac agggattat tacaacatg 
      aaggaagaa caattcaag caggtgttc agtgacatg 
      gatttcatt ggttggtac accaccgga gacatcccc 
      aacaccact gacattaaa atccataag caaatatgc 
      gacattaat gaatctcca attttgctt aaattaaat 
      ccctacgac aaaaacatt gatcatttg ccggttgct 
      ctttacgag tctgtaatt gatttgatt gggggcgaa 
      gcaacaatg ttatttgtt aatttgtct tatactctg 
      gcaaccgaa gaagcagaa agaattgga gtagatcat 
      gtggcgaga atgtcttca acggactca ggagaaagc 
      tctcttgtt gcagagcat ttgacagcg caacacagt 
      gctatcaaa atgttgcat tctagagtt aggctggtt 
      ttagagtat atgaaagct gtacaaagt ggacagttg 
      cctacaaat cacgagatt ttacgagag gcttattcg 
      ttgtgtcac cgattacct gttatacaa agtgcaagg 
      tttcgacaa gatttttat aatcaatgc aacgatgtg 
      ggccttatg acgtattta gggacttta acaaagggc 
      tgtaatgat ctcaaccag tttgtgaac aaattcaac 
      attttgtat gacagacaa ggactgggc aggaggatg 
      agaggaatt ttcttttaa
    mRNA sequence
    • >TC002246 RA
      gtgttattt atacatttt gattcaatt tcttgtaat 
      aattaatat gagtacgaa agaatcaat ggaaatgga 
      agtcgataa tccggcttc tgctgatgt tgcaaataa 
      tccggcgga agattcgga aaaaagtgt gatggcagc 
      tggggctgt cggttctgt gacttgttc attgcaccc 
      tttggtgat aatgaatgt ttctgagca ctggacacg 
      tgaaaaggc tcaagaggg ctcagtcca acgagtgat 
      tggggcttt gattggtaa acagaaggg gcgaaacat 
      tgaaataat gaactcatt tgaactggt atttagtgt 
      aattggagg ggatgttat tatcgacag ggattatta 
      caacatgaa ggaagaaca attcaagca ggtgttcag 
      tgacatgga tttcattgg ttggtacac caccggaga 
      catccccaa caccactga cattaaaat ccataagca 
      aatatgcga cattaatga atctccaat tttgcttaa 
      attaaatcc ctacgacaa aaacattga tcatttgcc 
      ggttgctct ttacgagtc tgtaattga tttgattgg 
      gggcgaagc aacaatgtt atttgttaa tttgtctta 
      tactctggc aaccgaaga agcagaaag aattggagt 
      agatcatgt ggcgagaat gtcttcaac ggactcagg 
      agaaagctc tcttgttgc agagcattt gacagcgca 
      acacagtgc tatcaaaat gttgcattc tagagttag 
      gctggtttt agagtatat gaaagctgt acaaagtgg 
      acagttgcc tacaaatca cgagatttt acgagaggc 
      ttattcgtt gtgtcaccg attacctgt tatacaaag 
      tgcaaggtt tcgacaaga tttttataa tcaatgcaa 
      cgatgtggg ccttatgac gtatttagg gactttaac 
      aaagggctg taatgatct caaccagtt tgtgaacaa 
      attcaacat tttgtatga cagacaagg actgggcag 
      gaggatgag aggaatttt cttttaatt cgtaaaaaa 
      agcaatttt atttataac cctcagttc tgtattaaa 
      gtttctatt aaacaatcc ttccatatt acaaattgt 
      cttttattg tattttgcg gcaattttt atcaaaaga 
      gcttataaa aggcaatga tacttgtat aaaaatttt 
      gggaagact acagtttgt aatttcttc ttcacttga 
      gtggtgatt gtgtgcggt acagagaga tgtttgtga 
      tttttatca cagaagcaa tttttaaat aactgttat 
      taacacaac acggtgaat acacaaaaa atctccctg 
      tttttacaa aatatttgc taactgctg cttctaaac 
      cccacatta gtatagaag tggtttttt tacacaat