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Gene information
General information
TC002241 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid36: 646670..648129
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002241
    iBeetle screen iB_03330
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002241 RA
      MSTGMKMCF TCRKVECGQ FINVRDYDT DKIFFLEKL 
      KTCITNLVE TDNMEMCPI CVKLLRISY KFHMQVLTS 
      QDILKRESF KDESDRIFN TSDDFEVKD DPDTFLSTS 
      DDFVKSDCD SDNSANKKP KRRATKKTF LCDHCASEY 
      PTKWQLRKH LARVHDDFR KFVCNYCGK EFKQSYHLK 
      EHITSHTGE KNYSCPICE KTFQRLSSQ RRHVKSHDA 
      PPGHKTKRT PFLCTICGK SFPFSNGVQ RHMRIHLGI 
      KNFECSFCN RRFTQSTHL HVHMRTHTG EKPYICDTC 
      GEKFSLKSC LLKHISTRH CKADNLANE MPIFFLTKE 
      GFEQS
    CDS sequence
    • >TC002241 RA
      atgagcact gggatgaaa atgtgtttc acgtgtcgt 
      aaagtcgaa tgcgggcaa tttatcaat gtgcgagat 
      tatgatacc gataaaatt ttcttcttg gaaaaattg 
      aaaacttgc ataacaaac ttggttgag actgataac 
      atggagatg tgccccatt tgtgtaaaa ttgcttcgg 
      attagttac aaatttcat atgcaagtc ctaacatct 
      caagatata ttgaaaagg gagtcattt aaagacgaa 
      tctgataga attttcaat acaagtgac gattttgaa 
      gttaaagac gatcctgat acatttttg agtacaagt 
      gacgatttc gtgaaatcg gattgtgac agtgataac 
      agtgcaaat aaaaaaccc aaaagacgt gccactaaa 
      aaaacattt ctttgcgat cattgcgcg tctgaatac 
      cccacgaaa tggcaattg cgcaaacac ttggcgcga 
      gttcatgac gattttcgc aaatttgtg tgcaactat 
      tgcggcaaa gaattcaaa cagtcgtac caccttaaa 
      gagcacatt acgtcccac acgggtgag aaaaactac 
      tcgtgcccc atttgtgag aaaacgttt caaagactg 
      tcgtcgcaa aggcgccac gttaagtcg cacgacgcg 
      cccccaggt cataaaacg aaacggacg ccgtttttg 
      tgcacgatt tgcgggaag agttttccg ttttcgaat 
      ggtgtgcag aggcatatg cggatccac ctcggaatt 
      aaaaacttt gagtgttcg ttttgtaat cggcgattt 
      acgcagtcg acgcatttg cacgtgcat atgaggacg 
      cacacgggc gaaaaaccg tacatttgt gacacgtgt 
      ggtgagaag ttttcgctt aagtcgtgt ttgcttaag 
      cacataagt acgaggcat tgtaaggcc gacaatttg 
      gccaatgaa atgccgata ttttttttg acgaaggaa 
      ggatttgaa cagagttaa
    mRNA sequence
    • >TC002241 RA
      cggggcctg aatatgtaa ctggagaca attaagctt 
      ttattggtt ttgctgcca gatttttta aattgaaga 
      cgcgcccgc gcgtttcgc tcgatcgat aaagtagaa 
      actctgttt taaaacaga cagtatcca aataacctc 
      aaaacgcca cttttcaaa caatttaaa caaaatcaa 
      ttaaaaatg agcactggg atgaaaatg tgtttcacg 
      tgtcgtaaa gtcgaatgc gggcaattt atcaatgtg 
      cgagattat gataccgat aaaattttc ttcttggaa 
      aaattgaaa acttgcata acaaacttg gttgagact 
      gataacatg gagatgtgc cccatttgt gtaaaattg 
      cttcggatt agttacaaa tttcatatg caagtccta 
      acatctcaa gatatattg aaaagggag tcatttaaa 
      gacgaatct gatagaatt ttcaataca agtgacgat 
      tttgaagtt aaagacgat cctgataca tttttgagt 
      acaagtgac gatttcgtg aaatcggat tgtgacagt 
      gataacagt gcaaataaa aaacccaaa agacgtgcc 
      actaaaaaa acatttctt tgcgatcat tgcgcgtct 
      gaatacccc acgaaatgg caattgcgc aaacacttg 
      gcgcgagtt catgacgat tttcgcaaa tttgtgtgc 
      aactattgc ggcaaagaa ttcaaacag tcgtaccac 
      cttaaagag cacattacg tcccacacg ggtgagaaa 
      aactactcg tgccccatt tgtgagaaa acgtttcaa 
      agactgtcg tcgcaaagg cgccacgtt aagtcgcac 
      gacgcgccc ccaggtcat aaaacgaaa cggacgccg 
      tttttgtgc acgatttgc gggaagagt tttccgttt 
      tcgaatggt gtgcagagg catatgcgg atccacctc 
      ggaattaaa aactttgag tgttcgttt tgtaatcgg 
      cgatttacg cagtcgacg catttgcac gtgcatatg 
      aggacgcac acgggcgaa aaaccgtac atttgtgac 
      acgtgtggt gagaagttt tcgcttaag tcgtgtttg 
      cttaagcac ataagtacg aggcattgt aaggccgac 
      aatttggcc aatgaaatg ccgatattt tttttgacg 
      aaggaagga tttgaacag agttaaagg gctgacaat 
      gtttccgta aattgtcaa attttgagg agaaatgta 
      cttatttac agcaataca ggctgatcc ttttagggc 
      ctatgtttg taatttttc aacttttaa tgtaactag 
      agctttgaa atttttaca caatctaat tcgaccatt 
      ctgacttca actaaatcg ttgttactg cctttttgg 
      agttgctat tttaaagtg aataaatgt attagctgt 
      tt