General information | |||
TC031264
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007423.3: 1036914..1089520 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC031264 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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GO ID | * {{ blurIdResult }} |
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Evidence | {{ blurEvidenceResult }} |
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Reference | * {{ blurReferenceResult }} |
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Quotation | ||
Name | ||
Publish name | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_03324 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)adult lethality - Number of animals showing the phenotype: 8 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_03323 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC031264 RA
MSYIEDANE TDSNNTSLD AFYFFETEQ FTLLWVLFV IIVAGNVGV LYTLLFGRS RKSRMNYFI THLALADLS VGLINVLTD IIWKTTVAW YAGNVACKV IRFLQVVVT YASTYVLVA LSIDRYDAI RHPMKFSGS WKRARYLIL AAWFFSALY SLPILIFYE EKIVKGKLQ CWIEFHPQW KWQVYMTLV SLSLFIIPA SIIATCYAI IIITIWSKN AKGFIKNTK AVNGSDNSR RASSRGLIP RAKVKTIKI TFVIVSVFI LCWSPYIIF DLLQVFGQI PGTQTNIAI ATFIQSLAP LNSAANPII YCLFSTHFC RTLGSLPPF KWLFKKKKR RNRESTTNT QSSSLSEFL TNTHKRKIE NTSTTFNAH TTCAVLFYL TSVFNSVLN LNEDRSYWL DVGKNDINR ALNLEKIEN PSKNVILFV GDGMGLTTT TASRIYSKS ESGFLSFEQ FPHIATIKT YNADKLVPD SSSTATALF SGVKNNLKT SGVDSSVKL NDCEASLRP EARIDGLMK WAQEAGKST GFVTTTRVT HATPSALYA HTPNRNWEC ESKIPEKSK QCKDIARQL VEDEPGRNI NVVMGGGRQ CLQSGVEDK PGDLVDRWA CYSLDGRDL IRDWEMDKK KRNKTYATV LNSEELKAV DTSNEFVLG IFANGHMSM DYARDKSPT GMPSLTNMT QKAIEILSQ NPEGYILVV EGGLIDFAH HRGNARKAL DETVSLSDA VQIAVEKTD PRETLIIVT SDHSHSLVF TGYPSRNKS VLDIAQKSK IDHIPYTSL LYGTGGPNN YQYSVINNT ISRQDPSIN NTSDFEYSQ QAVVLTDEV THGGSDVLI YAKGPMSHL FHSVHEQTY VAYVISYAA KIGPFKNGS SSFIYTKQI LKCSKIALF TRLYSSRNL LKLKERGMY QDIFPDTAT NTVTDLLNA APQRVYAGF DPTADSLHI GNLLVLINL IHWQRGGHQ PIALIGGAT AKIGDPSGK TSEREKLAS AFVDENVIG IRNNIETLF YNHEKYLWK KKGKLAPLI IVNNESWYS KMSPIDLIG GAGRYLRMG TLLSRTSVQ TRLQSPAGM SFTEFSYQL FQAYDWLHL FQKYNCSFQ IGGNDQMGN IMSGHELIS KTCDKQVYA LTVPIITTE MGDKFGKSA GNAMWLSAE KTSPFTLYQ FFVRQVDTE VEKMLKFFT FEPVGSIKD LMRRHTEKP ELRLPQKYL AEQVVLLVH GEKGLEAAH KATKVLYER SITALAEMN PLEIKSLFE GATVVEILP EPGQSVLDL AMSAGCFLT KEDALRIIS AGGFYINHQ KTNNPNEVL NLNVHRLSN NVTLLRVGK KNYYVVKWL S
- >TC031264 RA
atgtcatac attgaagat gcaaacgag accgattcg aacaacaca tcgttagac gctttttac tttttcgag accgaacaa ttcacctta ctatgggtc ctattcgtg ataatagtc gctggaaac gtaggtgtg ttgtacacg cttctcttt ggccgatct agaaaaagt cgaatgaat tatttcata acccaccta gccctagca gaccttagt gtgggtttg attaatgtt ttgacagat ataatttgg aaaacaaca gtggcatgg tatgccgga aacgttgcc tgtaaagtg atacgtttt ctccaggta gtggtaaca tatgcctcg acctatgtt ttggttgct ttaagtata gaccgatat gacgcaatt cgtcacccc atgaaattt tcaggcagt tggaaacgt gctagatat ctaatatta gccgcttgg ttcttcagt gcgctgtat tcgttaccg attttgatc ttctacgag gagaagatt gtgaaagga aaacttcaa tgttggatt gaatttcat ccacaatgg aaatggcaa gtctacatg acactagtt tcgctctca ctctttatc attcctgcg tcaattatt gcgacgtgt tacgccatc attattatc acaatttgg tcgaaaaat gccaaaggt ttcatcaaa aataccaaa gctgttaat gggtctgat aattcaaga agggcaagt tcgagaggt ttaatacca agagccaaa gtcaagact ataaaaatc acatttgtt atagtgagc gtttttatt ctatgttgg agtccttat ataattttt gatttgtta caagtgttt gggcaaata ccagggact cagaccaat atagctatc gctactttt atccaaagt ttagcacca ctcaactct gcagctaac cccattatt tactgcctc ttttctaca cacttctgt cggacattg gggtcattg cctccgttc aaatggttg ttcaagaaa aagaaaaga aggaatagg gaatcaaca acaaacaca caaagtagt tcattatcc gaattctta accaacact cacaaacga aaaatcgaa aatacatca acaactttt aacgcccat acaacatgt gcagtatta ttctatttg acaagcgtg ttcaacagt gtacttaat ttaaatgaa gacagatct tactggttg gatgtagga aaaaacgat atcaacaga gctcttaac ttagaaaaa attgaaaac ccttcaaaa aacgttata ttgtttgtt ggtgatgga atgggctta acaactact acagcatca agaatttac tccaagtct gaaagtggg tttttgtct tttgagcaa tttcctcat atagcaacg ataaagact tacaacgct gacaaattg gttcccgat tcaagcagt acagcgacg gctcttttt tctggtgtg aaaaacaat ttgaaaacg tcaggcgtt gattctagt gtgaaatta aacgactgt gaagcctca cttagacca gaagcgcgg attgacggc ttaatgaaa tgggcacaa gaggctgga aaatcaact ggattcgta acaacgaca cgcgtcaca catgccacc cccagtgca ctttacgca cacaccccc aacagaaac tgggagtgt gaaagcaaa attcccgaa aaatcgaaa caatgcaaa gacattgcg agacaattg gttgaggat gagccgggg agaaatatt aatgtagtg atgggtgga ggaagacag tgtttacaa tcgggggtt gaagataaa ccaggtgat cttgttgat agatgggca tgttatagt ttagatggg cgagatttg ataagagac tgggagatg gacaaaaag aaaagaaat aaaacttat gccactgtg ttgaatagt gaagaattg aaggcagta gacaccagt aatgaattt gttttagga atttttgca aatggacac atgtcaatg gattacgcc cgagataaa tcaccaact ggaatgcct tcactcacg aacatgaca caaaaagca attgaaatt ctaagccaa aacccagaa ggatatatt ttggtagta gaaggtggt cttatcgat tttgcccat cacagaggc aacgctaga aaagctcta gacgagact gtctcactt agtgatgca gttcagata gctgtcgaa aaaacggat ccacgagag acattaatt attgtaact agtgatcac tcgcattct ttggtattt actggatat ccttcaagg aacaaaagt gtattagac attgcacaa aagtcaaaa attgaccac ataccctac acttcgtta ctatatgga actggaggt ccaaacaat tatcaatac tccgttatt aacaatacc atttcaaga caagatcct tcaattaac aatacttca gattttgaa tattcacaa caagctgta gttctaacg gatgaagtg actcatggt ggaagcgat gttttaatc tatgctaaa ggtccgatg tctcattta ttccacagt gtacatgaa cagacgtat gttgcttac gtaattagt tacgccgca aaaattgga ccatttaaa aatggaagt tcatccttt atctacacg aaacaaatt ttaaaatgc agcaagatt gcgcttttc acgcgactc tattccagc cgaaacctt ctaaaattg aaagagcgc ggaatgtac caagacatt ttcccagat acagcaaca aacacggtt acggattta ttaaatgca gccccacag cgtgtttat gcaggcttt gaccccact gcagacagc cttcatatc ggaaattta cttgttctt attaattta atacactgg caaaggggt ggccaccaa cccatagca ctgattgga ggtgctacc gctaaaatt ggcgatccg agtggtaaa acatcagaa agagaaaaa ttagcaagt gcttttgtt gatgagaat gtgattgga attagaaac aacattgaa acgcttttt tataatcat gaaaaatac ctatggaag aagaaggga aaactggct cctctaata atcgtgaat aacgaatct tggtatagt aaaatgtca ccaatcgat ttgattggt ggtgctggt cgttatttg cgaatggga acattatta agcagaaca agtgttcaa acacgcctt cagtctccc gctggtatg agttttacc gaatttagt taccaatta tttcaagca tatgattgg ctgcattta tttcaaaaa tacaattgc tcttttcaa attggtgga aatgatcaa atgggcaat attatgtcg gggcatgaa ctcatcagc aaaacgtgc gacaaacaa gtttatgct cttactgtt ccgataatt acaaccgaa atgggcgac aaattcggc aaatctgca ggaaacgcc atgtggcta tctgctgaa aaaacctca cctttcact ctttaccag tttttcgtc cgtcaagtt gacaccgaa gttgaaaaa atgttaaag ttcttcaca tttgaacca gtcggaagc attaaagat ctgatgaga cgccacacc gaaaaacct gaattgcga ctacctcaa aaatatctt gctgagcaa gtggtgctc ttagtgcat ggggaaaag ggtttagag gctgcgcat aaagcgaca aaagtttta tacgaacga agcattaca gcactcgct gaaatgaat cctttggaa ataaagagt ttatttgaa ggagccaca gttgtggaa attttacca gagcctgga caaagtgtg ctagatttg gcgatgagc gctggatgt tttcttacc aaagaagat gcactgaga atcatttca gccggcggt ttttacatc aaccatcaa aaaacaaac aatcctaat gaagtttta aacttaaat gtacatagg ttaagcaat aatgtaacg ttacttcga gtaggcaaa aagaactat tatgtagta aaatggctt tcataa
- >TC031264 RA
tctccgaat ttttgtgct agaaacggg caacatgta acaatttct tgagacatg tcatacatt gaagatgca aacgagacc gattcgaac aacacatcg ttagacgct ttttacttt ttcgagacc gaacaattc accttacta tgggtccta ttcgtgata atagtcgct ggaaacgta ggtgtgttg tacacgctt ctctttggc cgatctaga aaaagtcga atgaattat ttcataacc cacctagcc ctagcagac cttagtgtg ggtttgatt aatgttttg acagatata atttggaaa acaacagtg gcatggtat gccggaaac gttgcctgt aaagtgata cgttttctc caggtagtg gtaacatat gcctcgacc tatgttttg gttgcttta agtatagac cgatatgac gcaattcgt caccccatg aaattttca ggcagttgg aaacgtgct agatatcta atattagcc gcttggttc ttcagtgcg ctgtattcg ttaccgatt ttgatcttc tacgaggag aagattgtg aaaggaaaa cttcaatgt tggattgaa tttcatcca caatggaaa tggcaagtc tacatgaca ctagtttcg ctctcactc tttatcatt cctgcgtca attattgcg acgtgttac gccatcatt attatcaca atttggtcg aaaaatgcc aaaggtttc atcaaaaat accaaagct gttaatggg tctgataat tcaagaagg gcaagttcg agaggttta ataccaaga gccaaagtc aagactata aaaatcaca tttgttata gtgagcgtt tttattcta tgttggagt ccttatata atttttgat ttgttacaa gtgtttggg caaatacca gggactcag accaatata gctatcgct acttttatc caaagttta gcaccactc aactctgca gctaacccc attatttac tgcctcttt tctacacac ttctgtcgg acattgggg tcattgcct ccgttcaaa tggttgttc aagaaaaag aaaagaagg aatagggaa tcaacaaca aacacacaa agtagttca ttatccgaa ttcttaacc aacactcac aaacgaaaa atcgaaaat acatcaaca acttttaac gcccataca acatgtgca gtattattc tatttgaca agcgtgttc aacagtgta cttaattta aatgaagac agatcttac tggttggat gtaggaaaa aacgatatc aacagagct cttaactta gaaaaaatt gaaaaccct tcaaaaaac gttatattg tttgttggt gatggaatg ggcttaaca actactaca gcatcaaga atttactcc aagtctgaa agtgggttt ttgtctttt gagcaattt cctcatata gcaacgata aagacttac aacgctgac aaattggtt cccgattca agcagtaca gcgacggct cttttttct ggtgtgaaa aacaatttg aaaacgtca ggcgttgat tctagtgtg aaattaaac gactgtgaa gcctcactt agaccagaa gcgcggatt gacggctta atgaaatgg gcacaagag gctggaaaa tcaactgga ttcgtaaca acgacacgc gtcacacat gccaccccc agtgcactt tacgcacac acccccaac agaaactgg gagtgtgaa agcaaaatt cccgaaaaa tcgaaacaa tgcaaagac attgcgaga caattggtt gaggatgag ccggggaga aatattaat gtagtgatg ggtggagga agacagtgt ttacaatcg ggggttgaa gataaacca ggtgatctt gttgataga tgggcatgt tatagttta gatgggcga gatttgata agagactgg gagatggac aaaaagaaa agaaataaa acttatgcc actgtgttg aatagtgaa gaattgaag gcagtagac accagtaat gaatttgtt ttaggaatt tttgcaaat ggacacatg tcaatggat tacgcccga gataaatca ccaactgga atgccttca ctcacgaac atgacacaa aaagcaatt gaaattcta agccaaaac ccagaagga tatattttg gtagtagaa ggtggtctt atcgatttt gcccatcac agaggcaac gctagaaaa gctctagac gagactgtc tcacttagt gatgcagtt cagatagct gtcgaaaaa acggatcca cgagagaca ttaattatt gtaactagt gatcactcg cattctttg gtatttact ggatatcct tcaaggaac aaaagtgta ttagacatt gcacaaaag tcaaaaatt gaccacata ccctacact tcgttacta tatggaact ggaggtcca aacaattat caatactcc gttattaac aataccatt tcaagacaa gatccttca attaacaat acttcagat tttgaatat tcacaacaa gctgtagtt ctaacggat gaagtgact catggtgga agcgatgtt ttaatctat gctaaaggt ccgatgtct catttattc cacagtgta catgaacag acgtatgtt gcttacgta attagttac gccgcaaaa attggacca tttaaaaat ggaagttca tcctttatc tacacgaaa caaatttta aaatgcagc aagattgcg cttttcacg cgactctat tccagccga aaccttcta aaattgaaa gagcgcgga atgtaccaa gacattttc ccagataca gcaacaaac acggttacg gatttatta aatgcagcc ccacagcgt gtttatgca ggctttgac cccactgca gacagcctt catatcgga aatttactt gttcttatt aatttaata cactggcaa aggggtggc caccaaccc atagcactg attggaggt gctaccgct aaaattggc gatccgagt ggtaaaaca tcagaaaga gaaaaatta gcaagtgct tttgttgat gagaatgtg attggaatt agaaacaac attgaaacg cttttttat aatcatgaa aaataccta tggaagaag aagggaaaa ctggctcct ctaataatc gtgaataac gaatcttgg tatagtaaa atgtcacca atcgatttg attggtggt gctggtcgt tatttgcga atgggaaca ttattaagc agaacaagt gttcaaaca cgccttcag tctcccgct ggtatgagt tttaccgaa tttagttac caattattt caagcatat gattggctg catttattt caaaaatac aattgctct tttcaaatt ggtggaaat gatcaaatg ggcaatatt atgtcgggg catgaactc atcagcaaa acgtgcgac aaacaagtt tatgctctt actgttccg ataattaca accgaaatg ggcgacaaa ttcggcaaa tctgcagga aacgccatg tggctatct gctgaaaaa acctcacct ttcactctt taccagttt ttcgtccgt caagttgac accgaagtt gaaaaaatg ttaaagttc ttcacattt gaaccagtc ggaagcatt aaagatctg atgagacgc cacaccgaa aaacctgaa ttgcgacta cctcaaaaa tatcttgct gagcaagtg gtgctctta gtgcatggg gaaaagggt ttagaggct gcgcataaa gcgacaaaa gttttatac gaacgaagc attacagca ctcgctgaa atgaatcct ttggaaata aagagttta tttgaagga gccacagtt gtggaaatt ttaccagag cctggacaa agtgtgcta gatttggcg atgagcgct ggatgtttt cttaccaaa gaagatgca ctgagaatc atttcagcc ggcggtttt tacatcaac catcaaaaa acaaacaat cctaatgaa gttttaaac ttaaatgta cataggtta agcaataat gtaacgtta cttcgagta ggcaaaaag aactattat gtagtaaaa tggctttca taataaaac ttacgtttt gtaacttct tttttttta attacgcct ggcggaaca gagaaaaac tggcttctc caaggtaag cattttcaa aatatttta taatttttt cttcaaaat agtttatta tcgttttta ataatgtta ttatagagt t
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
