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Gene information
General information
TC033918
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC002184
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid52: 324852..333184
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033918
    iBeetle screen iB_03320
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033918 RA
      MKYNVFRHI LIVLIFCGY VHSDCRMLH ICGEEDGKD 
      KNCNNDPNT PPETLNKTH DRYEEARQK LIEYCPFFF 
      ENNDDPELC CDVDQALNM IDGFRSTVP FQRCPACVT 
      NIINTYCIF SCSPDQYDF TLTLDYKND YVLKVQENI 
      DENYIQAVY DSCKNVANP STSSKLIEL VCGKYGAAY 
      CTPERFFNY MGSTSNGFA PIDIVFNSV NTSNSLNHT 
      ALRCNEAFE GYQACSCID CEASCSNSV QFEDLSYKD 
      FIFGNVQRD SFIAACVLL TIGFVSINF IVYFRIVKK 
      SLFPIFRKE NKKKTKTIQ AKISSYLET GFRNWGIFM 
      SKQRIIVLI ISILGIIAL TVGIIFLDV ITDPVELWA 
      APHSRSRQE KNFFDTNFQ PFYRTNQIF IKTVNIDPF 
      VYNQTIDLG NNKTDILSH TFGPAFNAT FLKEVFKLQ 
      TLIENITLG DGSGLESIC LAPLVTVFS GPKDISVCA 
      VQSLLGLFG NDASKLDED DYVETFIKC LSSPYSVDC 
      LAPYGGPVL PGLALGGNT FDNYTDAIA VSLTFLVEN 
      HLDENDLQA ALEWEEKFI DLMKRWDEF VKPEFMEIA 
      YSAERSIQD EIARTSSSE IGTVTISYV VMFIYIAFA 
      LGRYTSSDR FLVETKICV GIGGVLIVL GSVLASIGL 
      CGYAGISTT LLTIEVIPF LVLAVGVDN IFIIVQTHQ 
      RKQLNEKLS LEEEIGETM AKVGPSMLL TSCSEIFCF 
      AIGALSTMP AVNTFAIYA TFAIFFNFL LQITAFVAL 
      FTLDLKRYR ANRMEIFFC EKAKTSPED IGPGIVYKI 
      WKTNITPLI MNFPVRCVV LLLFLIWLC VSIAVMPSL 
      ELGLDQQLS MPEDSHVLT YFKFMNDLM GIGPPVYWV 
      AKGKVNYSV PENQAKMCG GIFCESNSI STQLYQAYR 
      QSNLTTMAT EASSWIDDF RDWANATDC CFYFKENGT 
      FCPHTLQRY CEPCNYGIM NLNESEYFT QFVSFFLMD 
      NPDAKCAKG GHASYASGI NFVLNQEGK ATIESSNMM 
      SYHTVLKGS TDYINALKY ARYIGDNLT KTLDIPDVE 
      IFPYSIFYT YFEQYLTIW EDALESLGL SLLVVFVVA 
      FAISGLNLF AACTTLIVV LMIVIDMMG LMYFWNINF 
      NAISLVNLV MSVGIAVEF CGHIVHHYI HSSKLSPLE 
      KASDAFAEM GSSVLSGIT LTKFSGIIV LAFARSQIF 
      QIFFFRMYL GIVIIGALH GLIFLPVLL SFLGFFERF 
      GRKQEIQNG DSSQL
    CDS sequence
    • >TC033918 RA
      atgaaatac aatgttttt cgacacatt cttattgtc 
      ttaatattc tgtggatat gtgcactcc gattgtcga 
      atgcttcac atttgtggg gaagaagat ggcaaagac 
      aaaaattgc aacaatgac ccgaacacc ccacccgaa 
      acattaaac aaaactcac gatagatat gaagaagct 
      agacaaaaa ctaattgaa tattgcccc ttcttcttc 
      gaaaataat gatgatccc gaattatgt tgtgacgta 
      gatcaagca cttaatatg atagacggt ttccgaagt 
      actgttccc ttccaaaga tgtcctgcc tgtgttacc 
      aacattata aatacttac tgcatattc tcctgttct 
      ccagaccaa tacgatttc acacttact ttggattat 
      aaaaacgat tacgtttta aaagtccaa gaaaatatt 
      gatgagaac tacattcaa gcagtttac gattcctgc 
      aaaaatgtt gcaaatcct agcacaagc agcaaactt 
      attgagcta gtttgtggc aaatatggt gctgcatac 
      tgtactccg gaacgattt ttcaattat atgggaagt 
      actagtaac ggatttgcg cccattgat atagttttt 
      aattcggtc aatacctca aactcactc aatcacaca 
      gccctcaga tgcaatgag gcttttgag ggatatcaa 
      gcatgtagt tgtattgac tgtgaagca agctgctca 
      aatagtgta caatttgaa gatttatct tacaaagac 
      tttattttt ggaaatgta caaagagat tcattcatt 
      gcagcttgt gtacttctc actattgga tttgtttcg 
      atcaatttt attgtgtac tttcgtata gttaaaaag 
      tcgcttttc cccatattt aggaaagaa aataagaag 
      aaaaccaag acgattcaa gcaaaaatc agcagttac 
      cttgaaact ggttttcgg aattgggga atatttatg 
      tctaagcaa cgaataata gttttaata atatcaatc 
      ctcggaatc attgccttg actgttggt attattttc 
      ttggatgtg attacggat cctgtagag ttgtgggca 
      gcaccacac agtcgaagt agacaagaa aaaaacttc 
      tttgataca aattttcaa cccttctat cgaacaaat 
      cagattttt attaaaacg gtaaacatc gaccctttt 
      gtttataat caaaccatt gatcttggt aacaataaa 
      accgatatt ttatcacat acgttcggt ccagcattc 
      aatgcaacc tttcttaaa gaagttttc aaactgcaa 
      actttaatc gaaaatata acgcttggt gatgggtca 
      ggactcgaa agcatatgt cttgctcct ttagtcact 
      gtattttcg ggacccaaa gatataagc gtttgtgcg 
      gtgcagtca cttttaggt ttgtttggg aacgatgcg 
      tctaaactc gatgaagat gattatgtg gaaactttc 
      atcaagtgt ttatcgagc ccctactct gtggactgt 
      ttggcacct tatggtggt cctgttctg ccaggtttg 
      gctttaggt ggaaatact tttgataat tacacagat 
      gcaattgct gtaagtcta acatttttg gtggaaaat 
      catttggat gaaaatgat ttgcaagca gcactcgag 
      tgggaggag aaatttatt gatttaatg aaacgttgg 
      gatgaattt gtaaaacct gaatttatg gagattgct 
      tacagtgcc gaaaggtcg attcaagac gaaatagca 
      cgaacatct tcatcagaa atcggaact gtaaccatc 
      agttatgtt gtaatgttt atttacatt gcttttgct 
      cttggtcga tacacctca agtgataga tttttggtt 
      gaaactaaa atatgtgtt ggaattgga ggagtactg 
      atagttttg ggatcagta cttgcttct attggtttg 
      tgtggctac gctggcatt tctacaaca ctgttaaca 
      attgaagta ataccattt cttgtgcta gctgttgga 
      gttgacaac atatttatt atcgttcaa acacatcaa 
      cgaaaacaa ctaaacgaa aagctaagc ttagaagaa 
      gaaattggc gaaaccatg gcaaaagta ggccccagc 
      atgcttttg accagttgt tccgaaatt ttctgtttt 
      gcgataggc gccctttcc acaatgcca gcagtcaac 
      actttcgcc atttatgca acatttgca attttcttc 
      aatttcctg ttgcaaatt actgctttc gttgcgctg 
      tttacatta gatcttaaa cgatataga gccaacaga 
      atggaaatt ttcttttgt gaaaaagcc aaaacatca 
      ccagaagac ataggacca ggaatagtt tacaaaatt 
      tggaaaaca aatataaca ccattgatc atgaatttt 
      ccagtgcga tgcgttgtc cttttatta tttttaata 
      tggctttgt gtgagtata gctgtaatg ccctccctt 
      gagctggga ttagaccaa caactctca atgcctgaa 
      gatagccac gttttaact tactttaaa tttatgaat 
      gatttgatg gggattggc ccacctgtt tattgggtg 
      gccaaggga aaagtgaac tattcggtg ccagaaaat 
      caggccaaa atgtgtgga gggattttt tgtgaaagc 
      aattcgatt tcgacacaa ctatatcaa gcgtataga 
      caatcaaac ctcaccaca atggccacg gaagcaagt 
      tcgtggatt gacgatttt cgagattgg gcaaatgct 
      acagattgt tgtttctat ttcaaagaa aatggaacg 
      ttttgtccc cacacactt caaagatat tgtgaacca 
      tgcaattat ggtatcatg aacttaaac gaaagtgaa 
      tattttacg cagtttgtg tccttcttt ttgatggat 
      aatcctgac gcaaagtgt gcaaaaggg ggtcatgca 
      tcttatgcc agtggaatt aactttgtt ttgaaccag 
      gaaggcaag gcaacaatt gagtcatcg aatatgatg 
      tcttatcat acggtttta aaaggttcg accgattac 
      attaatgca ttaaagtac gctcgatat atcggtgat 
      aacttaaca aaaacctta gatatacca gatgtcgaa 
      atttttccg tacagcatt ttctacaca tattttgaa 
      caatattta acaatatgg gaagatgct ttagaatct 
      ttgggatta tcacttctt gtagttttt gtagtagca 
      tttgcaata tctggacta aatcttttt gccgcttgt 
      acaaccctt attgtggtt ctgatgatt gtcattgat 
      atgatggga ttaatgtat ttttggaac attaacttt 
      aacgcaatc tcattagtt aatttagtc atgagtgtt 
      ggaatcgct gtggagttt tgtggtcat atagtccac 
      cattacatc cattcctca aaacttagc ccacttgaa 
      aaggcctct gatgctttt gctgaaatg ggaagctct 
      gttttatca ggaattaca ttgaccaaa ttttccggg 
      attatagtg ttagctttt gcacgctct cagattttt 
      caaatattt ttcttcaga atgtatcta ggaatcgtc 
      ataattgga gcattgcat ggattgatc tttttgcct 
      gttcttctg agttttctt ggatttttt gagaggttt 
      ggacgtaag caagaaatc caaaacgga gatagtagt 
      caactctga
    mRNA sequence
    • >TC033918 RA
      tgattctgg aaagtaaca aaaaacagt catgaaata 
      caatgtttt tcgacacat tcttattgt cttaatatt 
      ctgtggata tgtgcactc cgattgtcg aatgcttca 
      catttgtgg ggaagaaga tggcaaaga caaaaattg 
      caacaatga cccgaacac cccacccga aacattaaa 
      caaaactca cgatagata tgaagaagc tagacaaaa 
      actaattga atattgccc cttcttctt cgaaaataa 
      tgatgatcc cgaattatg ttgtgacgt agatcaagc 
      acttaatat gatagacgg tttccgaag tactgttcc 
      cttccaaag atgtcctgc ctgtgttac caacattat 
      aaatactta ctgcatatt ctcctgttc tccagacca 
      atacgattt cacacttac tttggatta taaaaacga 
      ttacgtttt aaaagtcca agaaaatat tgatgagaa 
      ctacattca agcagttta cgattcctg caaaaatgt 
      tgcaaatcc tagcacaag cagcaaact tattgagct 
      agtttgtgg caaatatgg tgctgcata ctgtactcc 
      ggaacgatt tttcaatta tatgggaag tactagtaa 
      cggatttgc gcccattga tatagtttt taattcggt 
      caatacctc aaactcact caatcacac agccctcag 
      atgcaatga ggcttttga gggatatca agcatgtag 
      ttgtattga ctgtgaagc aagctgctc aaatagtgt 
      acaatttga agatttatc ttacaaaga ctttatttt 
      tggaaatgt acaaagaga ttcattcat tgcagcttg 
      tgtacttct cactattgg atttgtttc gatcaattt 
      tattgtgta ctttcgtat agttaaaaa gtcgctttt 
      ccccatatt taggaaaga aaataagaa gaaaaccaa 
      gacgattca agcaaaaat cagcagtta ccttgaaac 
      tggttttcg gaattgggg aatatttat gtctaagca 
      acgaataat agttttaat aatatcaat cctcggaat 
      cattgcctt gactgttgg tattatttt cttggatgt 
      gattacgga tcctgtaga gttgtgggc agcaccaca 
      cagtcgaag tagacaaga aaaaaactt ctttgatac 
      aaattttca acccttcta tcgaacaaa tcagatttt 
      tattaaaac ggtaaacat cgacccttt tgtttataa 
      tcaaaccat tgatcttgg taacaataa aaccgatat 
      tttatcaca tacgttcgg tccagcatt caatgcaac 
      ctttcttaa agaagtttt caaactgca aactttaat 
      cgaaaatat aacgcttgg tgatgggtc aggactcga 
      aagcatatg tcttgctcc tttagtcac tgtattttc 
      gggacccaa agatataag cgtttgtgc ggtgcagtc 
      acttttagg tttgtttgg gaacgatgc gtctaaact 
      cgatgaaga tgattatgt ggaaacttt catcaagtg 
      tttatcgag cccctactc tgtggactg tttggcacc 
      ttatggtgg tcctgttct gccaggttt ggctttagg 
      tggaaatac ttttgataa ttacacaga tgcaattgc 
      tgtaagtct aacattttt ggtggaaaa tcatttgga 
      tgaaaatga tttgcaagc agcactcga gtgggagga 
      gaaatttat tgatttaat gaaacgttg ggatgaatt 
      tgtaaaacc tgaatttat ggagattgc ttacagtgc 
      cgaaaggtc gattcaaga cgaaatagc acgaacatc 
      ttcatcaga aatcggaac tgtaaccat cagttatgt 
      tgtaatgtt tatttacat tgcttttgc tcttggtcg 
      atacacctc aagtgatag atttttggt tgaaactaa 
      aatatgtgt tggaattgg aggagtact gatagtttt 
      gggatcagt acttgcttc tattggttt gtgtggcta 
      cgctggcat ttctacaac actgttaac aattgaagt 
      aataccatt tcttgtgct agctgttgg agttgacaa 
      catatttat tatcgttca aacacatca acgaaaaca 
      actaaacga aaagctaag cttagaaga agaaattgg 
      cgaaaccat ggcaaaagt aggccccag catgctttt 
      gaccagttg ttccgaaat tttctgttt tgcgatagg 
      cgccctttc cacaatgcc agcagtcaa cactttcgc 
      catttatgc aacatttgc aattttctt caatttcct 
      gttgcaaat tactgcttt cgttgcgct gtttacatt 
      agatcttaa acgatatag agccaacag aatggaaat 
      tttcttttg tgaaaaagc caaaacatc accagaaga 
      cataggacc aggaatagt ttacaaaat ttggaaaac 
      aaatataac accattgat catgaattt tccagtgcg 
      atgcgttgt ccttttatt atttttaat atggctttg 
      tgtgagtat agctgtaat gccctccct tgagctggg 
      attagacca acaactctc aatgcctga agatagcca 
      cgttttaac ttactttaa atttatgaa tgatttgat 
      ggggattgg cccacctgt ttattgggt ggccaaggg 
      aaaagtgaa ctattcggt gccagaaaa tcaggccaa 
      aatgtgtgg agggatttt ttgtgaaag caattcgat 
      ttcgacaca actatatca agcgtatag acaatcaaa 
      cctcaccac aatggccac ggaagcaag ttcgtggat 
      tgacgattt tcgagattg ggcaaatgc tacagattg 
      ttgtttcta tttcaaaga aaatggaac gttttgtcc 
      ccacacact tcaaagata ttgtgaacc atgcaatta 
      tggtatcat gaacttaaa cgaaagtga atattttac 
      gcagtttgt gtccttctt tttgatgga taatcctga 
      cgcaaagtg tgcaaaagg gggtcatgc atcttatgc 
      cagtggaat taactttgt tttgaacca ggaaggcaa 
      ggcaacaat tgagtcatc gaatatgat gtcttatca 
      tacggtttt aaaaggttc gaccgatta cattaatgc 
      attaaagta cgctcgata tatcggtga taacttaac 
      aaaaacctt agatatacc agatgtcga aatttttcc 
      gtacagcat tttctacac atattttga acaatattt 
      aacaatatg ggaagatgc tttagaatc tttgggatt 
      atcacttct tgtagtttt tgtagtagc atttgcaat 
      atctggact aaatctttt tgccgcttg tacaaccct 
      tattgtggt tctgatgat tgtcattga tatgatggg 
      attaatgta tttttggaa cattaactt taacgcaat 
      ctcattagt taatttagt catgagtgt tggaatcgc 
      tgtggagtt ttgtggtca tatagtcca ccattacat 
      ccattcctc aaaacttag cccacttga aaaggcctc 
      tgatgcttt tgctgaaat gggaagctc tgttttatc 
      aggaattac attgaccaa attttccgg gattatagt 
      gttagcttt tgcacgctc tcagatttt tcaaatatt 
      tttcttcag aatgtatct aggaatcgt cataattgg 
      agcattgca tggattgat ctttttgcc tgttcttct 
      gagttttct tggattttt tgagaggtt tggacgtaa 
      gcaagaaat ccaaaacgg agatagtag tcaactctg 
      acaaatgta tggagtttg agaagccat atgtaaata 
      tctatgaaa acttaagct tgtactttt gtatgaaag 
      gacaaaaga gttattctc ttatttaat aaagtatat 
      ttttatat