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Gene information
General information
TC002173 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 1094257..1095480
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC002173
      iBeetle screen iB_03319

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
      Days post injection


      Adult morphology
      remarks - ovaries: later than schedule,
      Fertility
      mature egg deposition blocked number 1st lateral oviduct: 2 number 2nd lateral oviduct: 3 - Number of animals showing the phenotype: 2
      ovary without detectable defects - Number of animals showing the phenotype: 2
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC002173 RA
        MCLENESDL LSHWFLDIG KKSNMYNEP MLGEKTVKF 
        FKQICDTFE QNTHVFIMS VEVAENYLR TKKSKNEKI 
        ADPLLAAVT ISFICHKCE GALESLKVK HIVDLLFQL 
        SKKSYSARQ VLGMEGDIL ITLNGIIQM TTVLDDMDV 
        IIEKYMSES RLKGSIRPL CLKILEYVY FERKKWFQQ 
        LKEIYSQNK ECMNVFQHL ISSKFYVPV SVLITAIEL 
        THYRHVLDV DTVLADVTK HTKIHEDHV RLFAAKIIE 
        VIKC
      CDS sequence
      • >TC002173 RA
        atgtgcctc gagaacgaa tcagattta ttatcacac 
        tggtttttg gacattggt aagaaaagt aatatgtac 
        aacgaacca atgcttggt gaaaagacc gtcaagttc 
        tttaaacaa atctgcgac acgttcgaa caaaacacc 
        cacgttttc atcatgtcc gtcgaagtg gctgaaaat 
        tacctccgt acgaagaaa tccaaaaac gagaagatt 
        gcggacccg cttctggca gctgtaacc atttcgttc 
        atttgccac aaatgcgaa ggagcgctc gaaagtctc 
        aaagtgaaa cacattgtg gacttgctg tttcaactg 
        tcaaaaaaa agttacagc gcgaggcaa gttctggga 
        atggagggc gatatttta atcactctc aatgggata 
        attcagatg acaacggtt ttggacgat atggacgtg 
        ataatagag aagtatatg agtgagagt agactaaaa 
        gggagcata cgccctttg tgtttgaaa atcctcgaa 
        tatgtttac ttcgaacgc aaaaaatgg tttcaacaa 
        ctcaaagag atctacagc cagaacaag gagtgtatg 
        aacgtgttc caacatctg atttcgtcc aagttctac 
        gttcctgta agtgtttta attacagca attgaatta 
        actcattat cgacacgtt ttggatgtg gacacggtc 
        ttggccgat gttaccaaa cacactaaa atccacgaa 
        gaccatgtg cgacttttt gccgctaaa ataatcgaa 
        gttataaag tgttaa
      mRNA sequence
      • >TC002173 RA
        agatccctt caaatacga ccctggccc attatgttt 
        ttgttacaa ctacaaaaa attattgaa aaactgtca 
        aataatcag tttctgtgc gtaaatatg atcacaatc 
        actttgaaa gagcagatc gagtttttt atttagctc 
        aaattacag gaagtgtca cccccgtcg tgttgttgg 
        tgccactgt ccgctacca ctttttgac acctttcaa 
        aagtgaaaa gtcatggtt tcaaaaatg tgcctcgag 
        aacgaatca gatttatta tcacactgg tttttggac 
        attggtaag aaaagtaat atgtacaac gaaccaatg 
        cttggtgaa aagaccgtc aagttcttt aaacaaatc 
        tgcgacacg ttcgaacaa aacacccac gttttcatc 
        atgtccgtc gaagtggct gaaaattac ctccgtacg 
        aagaaatcc aaaaacgag aagattgcg gacccgctt 
        ctggcagct gtaaccatt tcgttcatt tgccacaaa 
        tgcgaagga gcgctcgaa agtctcaaa gtgaaacac 
        attgtggac ttgctgttt caactgtca aaaaaaagt 
        tacagcgcg aggcaagtt ctgggaatg gagggcgat 
        attttaatc actctcaat gggataatt cagatgaca 
        acggttttg gacgatatg gacgtgata atagagaag 
        tatatgagt gagagtaga ctaaaaggg agcatacgc 
        cctttgtgt ttgaaaatc ctcgaatat gtttacttc 
        gaacgcaaa aaatggttt caacaactc aaagagatc 
        tacagccag aacaaggag tgtatgaac gtgttccaa 
        catctgatt tcgtccaag ttctacgtt cctgtaagt 
        gttttaatt acagcaatt gaattaact cattatcga 
        cacgttttg gatgtggac acggtcttg gccgatgtt 
        accaaacac actaaaatc cacgaagac catgtgcga 
        ctttttgcc gctaaaata atcgaagtt ataaagtgt 
        taagtttta ttatgacaa tagtactaa gtattgctt 
        aacccatgc acatttatt aagcttaaa aaactttgt 
        taggattgt ttgttttgt ttttgttta ttgtttttt 
        tttgtggtt tatgtaaaa actaccggt agaggtgtt 
        tcttattcc aacatctgt actaaaata ccaaaaatt 
        attattttg atttttaaa taaattgtt ttggaccat
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY