Status
Please wait ...
Gene information
General information
TC002171 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 1167277..1196849
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002171
    iBeetle screen iB_03318

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    labrum size decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    labrum shape irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    head appendages random not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    antenna sometimes shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002171 RA
      MCIKGVSQI RHCTRGLRD VRINVPSAV VRGDDATLQ 
      CYYDLEGEQ LYAVKWYLG TTEFYRYTP KEIPPIKQF 
      PSQSGLTVR EPDSNATSV LLEHVPRSI SGRYSCEVS 
      ADAPSFYTA IQSAYLQVV DLPPKDPYI SINKTRYHH 
      GEMLRATCT SEHSNPAVN LTWYVNGLP AESKYVHNS 
      REKSADGQY HTSHSVLKM KITKQSFVK HRLKIRCVA 
      RMYDIYYKS HEKSVESER KRHHHYFVT TTEPPVQWE 
      LVETLGANP EVRFVFNSS VGICFSSIV YIVMVLALF 
      R
    CDS sequence
    • >TC002171 RA
      atgtgtata aagggggtt tcccaaata aggcattgc 
      acgcgagga cttcgagat gtccgtatc aacgttccc 
      tcggcggta gttcgagga gatgacgcc actctgcag 
      tgttactac gacctggag ggcgagcag ctttatgcg 
      gtcaaatgg tatttgggc acaacggaa ttttaccgg 
      tatactccc aaagagatc cctccaatt aagcagttt 
      ccctcgcag agcggcctc accgtacgt gagcccgac 
      tccaacgcc acttcggtg ctcctcgag catgttcct 
      aggagtatt agtggacgc tacagttgc gaagtttca 
      gcagacgcg cctagtttc tacacagct attcagtcg 
      gcctatctg caggtggtg gatttgccg ccaaaagat 
      ccttacata agtatcaac aaaacacgc tatcatcac 
      ggagaaatg ctcagagct acgtgcact tcagaacat 
      tcaaatcct gcagttaat ctcacttgg tatgttaat 
      ggattgcct gcggaatct aagtatgtg cataatagc 
      agagagaaa tcagctgat ggacaatat cacacgtcc 
      cattcagtg ctgaagatg aagattacc aagcagtcg 
      tttgtaaag caccgcttg aaaatacgt tgcgtggct 
      agaatgtac gatatttat tacaagagc cacgagaaa 
      agtgtcgag tcggaaaga aaaaggcac catcattat 
      tttgttacg actacggag ccgccggtg cagtgggag 
      ctggtggag acgcttgga gcgaatcca gaagttaga 
      tttgtgttt aattcttca gttggtatc tgcttcagt 
      tcgatagtt tatattgtt atggtttta gccctgttt 
      agatga
    mRNA sequence
    • >TC002171 RA
      gggattcgc gatgtgtat aaagggggt ttcccaaat 
      aaggcattg cacgcgagg acttcgaga tgtccgtat 
      caacgttcc ctcggcggt agttcgagg agatgacgc 
      cactctgca gtgttacta cgacctgga gggcgagca 
      gctttatgc ggtcaaatg gtatttggg cacaacgga 
      attttaccg gtatactcc caaagagat ccctccaat 
      taagcagtt tccctcgca gagcggcct caccgtacg 
      tgagcccga ctccaacgc cacttcggt gctcctcga 
      gcatgttcc taggagtat tagtggacg ctacagttg 
      cgaagtttc agcagacgc gcctagttt ctacacagc 
      tattcagtc ggcctatct gcaggtggt ggatttgcc 
      gccaaaaga tccttacat aagtatcaa caaaacacg 
      ctatcatca cggagaaat gctcagagc tacgtgcac 
      ttcagaaca ttcaaatcc tgcagttaa tctcacttg 
      gtatgttaa tggattgcc tgcggaatc taagtatgt 
      gcataatag cagagagaa atcagctga tggacaata 
      tcacacgtc ccattcagt gctgaagat gaagattac 
      caagcagtc gtttgtaaa gcaccgctt gaaaatacg 
      ttgcgtggc tagaatgta cgatattta ttacaagag 
      ccacgagaa aagtgtcga gtcggaaag aaaaaggca 
      ccatcatta ttttgttac gactacgga gccgccggt 
      gcagtggga gctggtgga gacgcttgg agcgaatcc 
      agaagttag atttgtgtt taattcttc agttggtat 
      ctgcttcag ttcgatagt ttatattgt tatggtttt 
      agccctgtt tagatgatt cctacaaaa tatagaatt 
      acataatgt accgaaata acttccacg aattttatg 
      tgatacaat aattattat aataaatgt ggtttgtaa 
      ataaatgct attgtataa atgttgatg ttttttgga 
      caaagctat taacgcgcg aggttgtct tttattgta 
      aaagtttcg ctaaatcgg cgtttaatc tgcattatt 
      tgacgctca aacaatttt tttgggtaa ttatttcag 
      cctcatttt gttgatagc gattgtcta tgtgtatat 
      aatgtatct atgtacagt acagtagga cttcgatta 
      ttacaaacg ataagcgga taatttcaa aaactattt 
      caattttgt tgaagatgt ggaaagtag agaagaaat 
      ctgggcgcg ttggtttga cgaagactg aatttttca 
      aaatatcgg tttgtaaat attttatac aggtgcgaa 
      tctgtgatg aaaaggcaa aacccttta tgtccaaaa 
      tttatattt ccttcttaa ttccaatca atatagaaa 
      acatatcgg gttcttggc atcacagat ttttttgaa 
      aaattttac ttgttaaat agtaggcgt tttttacct 
      ggtttctaa atttatcga gtttacgac taaaaatcg 
      tttactgtg atgtaacag ataaatttt agagactag 
      tataaagcc aaaaatatc cactattaa tccaaaata 
      tattttcga aaataacga cctaaaacc gtgaccacg 
      aaaaagtac ttattttat atttaacat attgatttt 
      tttcctttt tttctgcgt tttaattac ctaaatacc 
      aggaggcga cacgaggta tatatacaa taaaaatgt 
      aaggaagaa agttgtaga gaattaaat tgtcttcaa 
      aaacaaact aagatttaa gctgattta aaggttcct 
      caggcaatc aacggtaaa aatgtaacg caatgtact 
      aaattacca gaatcatta aaatgggaa tcttattaa 
      aacattagg atgaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY