Status
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Gene information
General information
TC031745
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC002165,TC002166
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450989.1: 88603..116410
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031745
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03317

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031745 RA
      MEPNKFKKR AGTTDFGKK YEDLNLARL VLKLVIDDT 
      VDDFTISSN DEKFGDFDD IVIELKSDK SFTKTAIQI 
      KYSNEGKTL PVSSFWCEK GDFSLKKYL RSYKQIKQY 
      KLILFTNRK LDLAHENSF NFENISLRI VQEQVDQLV 
      NSAKHDGRC YKFEANEAS DSNLEILGF FENFRIFTN 
      QSSVSVIEK MTLDEFQKI FHCPSEHFH TYLQFITRW 
      SLLEGNKEK LGKELMKRA IALNVLSPF IQPLVPSLI 
      DEKMILLRK TIAKFQVTV FRKKSYDKI KGLWGDFYQ 
      KLGQNNNDN KIKSRFQLD QDDSFGSKM LWLKSDAPL 
      IVVESVTTS RIIQMCNDW FIVLGNGPI LPQNRTVFR 
      HLADLNQGS EMYKLTCCV QGKEQQLFE TFFDNEMRQ 
      VVTTDDLLQ LLSQEVYSF GGKQETLPV PYIERFLTG 
      HVIRFEHLH KMDSLVVIN CGKKLETIT KRNKIKSLL 
      RGLNAINGS DYLKDTCYH KSDCDLLKP KLFLFEDKC 
      SQQDFDKIR ELNKYETIH HFSVVEDDE HLEWLKSNK 
      NATDLKEFL IKSVTINES LIFDKSGVN LIIGEPGIG 
      KTIFVKNLK KHNSPNIWS VILSPSVFQ NPFNRHTFE 
      EVILAQTHT NLDRKILQI LMKKNRVVY IVDSWDQIS 
      LEQLTCICD FFRTEELPL WVTSRPHLQ NFFETKLNV 
      LSRTLLPFS DVQKKSYIC KRLEEKYPD IGTEVLKIE 
      TYINQEILN NPLHIYMLT EVFSLDDDK FSQLSADLF 
      NVCDLYEYW VEQKFALYY NQKFGVWAN DRVFEEHKN 
      YLKRDYQLA ALHRNAEFL EKVKIEGDP IGIIEVTDD 
      QSPKFFHPS IAEYFIASH LAKNFAQVS SDFIFKEEN 
      RNVRYFFDL LLAQNSKPH MAVFYRNLD VLKKIQNLD 
      KFVDSGGRN VLHLACSWG QTYPNFDVE TQEQDWIYL 
      VTPKETSEV LREDPNYKE MIVYLVEKL DLWQRDTLF 
      NYTALQYAE ASNCLYTKM IILRKMNKT IDLDTPMAS 
      LLYYAAEFG YEDVFNLYE TFPLIKLKD QSFLHLCVQ 
      HEHFLAQLL AIESYHKMI DFAPNGETA LHKCCKEGY 
      YKSALLLRQ NGASCVTTT SDGWTPLHF ASLNGHESI 
      VTLLKDVDV NTATNKGYT ALHLACYKG YTTIVTLLL 
      DLGANIYGN TNVTPLLLA CQNGQEDVA RCLLQLGAL 
      VTTSNSWTP LHSACYKGH ESIVKLLID FGASVTTVD 
      GKNVTPLHL ACHEGHETI VELLLNLRA NVDARDSVG 
      FTPLHLACH RNFEQIAKL LIAFGASCD VSDDMGLTP 
      LHWACQEGR DQIVELLLA SGANLETVT KTGKTPFHF 
      ACLKGHEAV VRLLMPLVN PQIATTRNF TPLHLACQE 
      GHENVVELL LQTGVNSVT QDGSTPLHW ASHNGHYNI 
      VKMLLQSGA KVEIRDSEG STPLLLACY QGFDKIAKL 
      LIHFGANIT TSNNRGFTP LHWASQQNH PNLVKVLIE 
      LGAKVTIGT QQGFTPLHL ACQKGHISV VKRLIVSGA 
      NIEDVTNKG WTPLHWASF KGHETVTNL LLGADANVN 
      IPNGEGMTP LHLACSKGF VQIANTLIE FGASTECEN 
      CDGLSPLYL ACQGGHLEV VKLLIMFGT IVGGERRAR 
      SREISLRYF MI
    CDS sequence
    • >TC031745 RA
      atggagccc aacaagttt aaaaaacga gctggaacc 
      accgacttt ggcaaaaaa tacgaggac ttgaactta 
      gctcgtttg gtcctaaaa ctggtaatt gacgacaca 
      gtcgacgat ttcacgatc tcgtcaaac gacgaaaag 
      tttggtgat tttgacgac attgttatt gaattaaaa 
      tcggacaaa agctttacc aaaacagct atacaaata 
      aaatacagt aatgaaggg aaaacctta ccggtgtca 
      agcttctgg tgcgaaaaa ggagacttt agtttgaag 
      aaatatcta agatcgtac aaacaaatc aaacaatac 
      aaattaata cttttcaca aaccggaaa ctggacctg 
      gcacatgaa aatagcttc aattttgaa aacatatcg 
      cttagaatt gtacaagaa caagtcgat caattggta 
      aattcggcc aaacatgac gggaggtgc tataagttt 
      gaagcgaac gaagcgagt gactcaaat ttggaaatt 
      ttgggtttt ttcgaaaat tttcgtatt tttacgaac 
      caatcgagc gtcagtgtg attgaaaaa atgactctt 
      gacgagttt caaaaaata tttcattgc ccctcggaa 
      catttccac acttatttg caatttatc accaggtgg 
      agtcttttg gaaggaaat aaggaaaag ttgggtaaa 
      gagttgatg aaacgtgcg atagcactc aacgtcttg 
      tctccattt attcaaccc ttggttccg tcattaatt 
      gacgaaaaa atgattttg ttgcgaaaa actatagca 
      aagtttcaa gtgacagtt tttcggaaa aaatcgtac 
      gacaaaatc aagggatta tggggcgat ttttaccaa 
      aaacttggc caaaataac aatgataat aaaatcaaa 
      tcaagattt caattggat caagacgat tcatttggt 
      agcaaaatg ttgtggtta aaatcggac gctccttta 
      atagttgtt gaaagtgtg accacatct cgcatcatc 
      caaatgtgc aatgactgg tttattgtg cttggaaac 
      ggtccgatt ttgccacaa aatcgtaca gttttccga 
      catttggct gatttaaat caagggtct gaaatgtat 
      aaactcact tgttgtgtg caagggaag gaacaacaa 
      ctttttgag acttttttc gataatgaa atgaggcaa 
      gttgtgaca actgatgat ttgttgcaa ttattgtca 
      caggaagtt tattcgttt ggtggcaaa caagagact 
      cttcctgtg ccttacatt gagaggttt ttgactgga 
      catgtgata cgttttgag catttgcac aaaatggat 
      tccttggtt gtgatcaat tgtgggaaa aagttggag 
      acaatcacc aaacgaaac aaaatcaag agtcttttg 
      aggggtctt aacgcaatc aatggttcg gattatttg 
      aaagacact tgttaccac aaaagtgat tgtgaccta 
      cttaagcca aaattattc ctttttgaa gataagtgt 
      tcccaacaa gactttgac aaaatcaga gaattgaac 
      aaatatgaa accattcac cactttagt gttgttgaa 
      gacgacgaa cacttggag tggctcaaa agcaacaaa 
      aatgcaact gacttgaaa gaattcttg atcaaaagt 
      gtgaccatc aacgaatct cttattttc gacaaaagt 
      ggtgttaat ttaataatt ggcgaacct ggcattggt 
      aaaacaatt tttgtcaag aatttaaaa aaacacaat 
      tcgccaaat atttggagc gttattttg tccccttct 
      gtgtttcaa aatcccttc aatagacac acttttgag 
      gaggttatt ttagcccaa acacacacc aaccttgac 
      aggaaaatt ctccaaatt ttgatgaag aagaaccga 
      gttgtgtac attgtggac tcttgggac caaatctcc 
      ttggaacaa ctcacttgc atttgtgat tttttccgt 
      actgaagag ttaccactt tgggttact tcacgtccc 
      cacttgcaa aactttttc gaaacgaag ctaaacgtt 
      ttgtcacga acgctttta cccttcagt gacgtccaa 
      aaaaaatca tacatttgc aaacgtctc gaagagaaa 
      tatccagac attgggaca gaagttctc aaaatcgaa 
      acatacata aaccaggag attctaaac aacccgtta 
      cacatctac atgttgacc gaagtcttt agtcttgat 
      gacgacaag ttttcgcaa ctttcggcc gatttgttc 
      aacgtctgc gacttgtat gagtattgg gttgaacaa 
      aaattcgcc ctgtattac aatcaaaag tttggtgtt 
      tgggccaat gatcgtgtt ttcgaagaa cacaaaaac 
      tacctcaaa cgcgactat caactagct gcgttacat 
      cgaaatgcc gaatttttg gaaaaagtg aaaattgaa 
      ggtgatccg attggtatc atcgaagta acagacgac 
      caatcccca aaatttttc cacccttcg attgccgaa 
      tatttcatc gcgtcccac cttgctaaa aattttgcc 
      caagtctcg tctgacttt attttcaaa gaagagaac 
      agaaatgta cgatatttc ttcgacttg ttactcgcc 
      caaaattcc aaaccacac atggcagta ttttaccga 
      aatttggac gtgttgaag aaaatccag aatttggac 
      aagtttgtt gattcaggg ggacgtaac gtgttacac 
      ttggcgtgt tcttggggc cagacatat ccaaatttt 
      gatgtggaa acgcaagaa caagactgg atttatttg 
      gttacaccg aaggaaacg agtgaagtg ttacgtgaa 
      gaccccaat tacaaagaa atgattgtt tatctagta 
      gaaaagttg gatttgtgg caacgtgac actttattc 
      aactatacg gcgttacaa tatgctgaa gcttccaac 
      tgtctatac accaaaatg attattttg cgaaaaatg 
      aacaaaaca atagatttg gacacaccg atggctagt 
      ctattgtat tatgcggcg gaatttggc tatgaagac 
      gtgttcaat ttgtacgaa acgtttccc ttgattaaa 
      ctgaaagac cagtcgttt ctgcacctt tgtgtgcaa 
      catgaacac ttcctagcc caacttttg gcaattgaa 
      agttaccac aaaatgatt gattttgca ccaaatggt 
      gaaacagcg ttacacaag tgttgcaag gaaggttac 
      tacaagagt gccttattg ttacgacaa aatggcgcc 
      tcctgtgtt actactaca agtgatggt tggacccct 
      ttacatttc gcatcacta aatggacac gaatctatt 
      gtaacactt ttgaaagat gttgatgta aacactgcc 
      actaacaaa ggttacact gcgttacac ttggcgtgt 
      tacaaaggt tacacgacc atcgtcacg ttactgcta 
      gatttgggg gcaaatatt tacggtaac acaaatgtt 
      acgccattg ttgttggct tgtcagaat ggacaagaa 
      gacgtagcc cgttgtttg ttacaattg ggggcactt 
      gtaactaca agtaatagt tggactcct cttcactca 
      gcttgttac aaggggcat gaaagcatt gtcaagtta 
      ttgatagac tttggggcc agtgttacc acagttgat 
      ggtaaaaat gtaactccg ttacatttg gcatgtcat 
      gaagggcat gaaacgatc gtggagtta cttctaaat 
      ttgagggca aatgttgat gccagagat agtgttggc 
      tttactccg cttcatttg gcttgtcat cgaaatttt 
      gaacaaatt gccaaattg ttgattgcg tttggtgcc 
      agttgtgat gtttcggac gatatgggt ttgactccg 
      ttacattgg gcgtgtcag gaagggcgt gatcaaata 
      gttgagtta cttctagca tctggtgca aaccttgaa 
      actgtgaca aaaacaggc aaaaccccc tttcacttt 
      gcatgttta aaaggacac gaagccgta gttcgcctt 
      ttgatgccg ttagttaac ccccaaatt gcaacaact 
      cgaaacttc accccctta cacctagca tgccaagaa 
      gggcacgaa aacgtagtt gaattgtta ctccaaact 
      ggtgttaac agtgtcaca caagacggc tcaacaccc 
      cttcattgg gcttcacac aatggacac tataacatc 
      gtcaaaatg ttactccaa tcgggggca aaagttgaa 
      attagggac agtgaaggc tcgacacca ctcctcctc 
      gcctgttat caaggtttt gacaagatt gccaaactt 
      ttgatacac tttggtgca aatattacc acaagtaac 
      aatcgtggt ttcacccct ttgcattgg gcaagtcaa 
      caaaatcat ccaaacttg gtcaaagtt ttaatcgaa 
      ttgggggcg aaagttaca atagggacg caacaaggg 
      ttcacccct ttgcattta gcgtgtcag aaagggcac 
      atttcggtt gttaaacga ttgattgtc tcgggtgca 
      aatattgaa gatgttaca aataagggg tggactcca 
      cttcattgg gcttctttc aaaggacat gaaaccgtc 
      acgaattta ttgctaggg gctgatgct aatgtcaat 
      attcctaat ggggaaggt atgacaccg ttacatttg 
      gcttgttcg aagggtttt gtccaaatt gcaaatacg 
      ttaatcgag tttggggcc agtactgaa tgtgagaat 
      tgtgatggt ttgtctccg ctttattta gcgtgtcaa 
      ggggggcat ttagaagtc gttaagttg ttgattatg 
      tttgggacg attgttgga ggagaaaga agggcgaga 
      agtagggaa atatcgctg cgttatttt atgatatga
    mRNA sequence
    • >TC031745 RA
      tgataataa tttgtttaa caagtgaga caataatta 
      taaaataaa aaatttaat ttggcagtg aattaaaac 
      acgaagctg taaacatat ggagcccaa caagtttaa 
      aaaacgagc tggaaccac cgactttgg caaaaaata 
      cgaggactt gaacttagc tcgtttggt cctaaaact 
      ggtaattga cgacacagt cgacgattt cacgatctc 
      gtcaaacga cgaaaagtt tggtgattt tgacgacat 
      tgttattga attaaaatc ggacaaaag ctttaccaa 
      aacagctat acaaataaa atacagtaa tgaagggaa 
      aaccttacc ggtgtcaag cttctggtg cgaaaaagg 
      agactttag tttgaagaa atatctaag atcgtacaa 
      acaaatcaa acaatacaa attaatact tttcacaaa 
      ccggaaact ggacctggc acatgaaaa tagcttcaa 
      ttttgaaaa catatcgct tagaattgt acaagaaca 
      agtcgatca attggtaaa ttcggccaa acatgacgg 
      gaggtgcta taagtttga agcgaacga agcgagtga 
      ctcaaattt ggaaatttt gggtttttt cgaaaattt 
      tcgtatttt tacgaacca atcgagcgt cagtgtgat 
      tgaaaaaat gactcttga cgagtttca aaaaatatt 
      tcattgccc ctcggaaca tttccacac ttatttgca 
      atttatcac caggtggag tcttttgga aggaaataa 
      ggaaaagtt gggtaaaga gttgatgaa acgtgcgat 
      agcactcaa cgtcttgtc tccatttat tcaaccctt 
      ggttccgtc attaattga cgaaaaaat gattttgtt 
      gcgaaaaac tatagcaaa gtttcaagt gacagtttt 
      tcggaaaaa atcgtacga caaaatcaa gggattatg 
      gggcgattt ttaccaaaa acttggcca aaataacaa 
      tgataataa aatcaaatc aagatttca attggatca 
      agacgattc atttggtag caaaatgtt gtggttaaa 
      atcggacgc tcctttaat agttgttga aagtgtgac 
      cacatctcg catcatcca aatgtgcaa tgactggtt 
      tattgtgct tggaaacgg tccgatttt gccacaaaa 
      tcgtacagt tttccgaca tttggctga tttaaatca 
      agggtctga aatgtataa actcacttg ttgtgtgca 
      agggaagga acaacaact ttttgagac ttttttcga 
      taatgaaat gaggcaagt tgtgacaac tgatgattt 
      gttgcaatt attgtcaca ggaagttta ttcgtttgg 
      tggcaaaca agagactct tcctgtgcc ttacattga 
      gaggttttt gactggaca tgtgatacg ttttgagca 
      tttgcacaa aatggattc cttggttgt gatcaattg 
      tgggaaaaa gttggagac aatcaccaa acgaaacaa 
      aatcaagag tcttttgag gggtcttaa cgcaatcaa 
      tggttcgga ttatttgaa agacacttg ttaccacaa 
      aagtgattg tgacctact taagccaaa attattcct 
      ttttgaaga taagtgttc ccaacaaga ctttgacaa 
      aatcagaga attgaacaa atatgaaac cattcacca 
      ctttagtgt tgttgaaga cgacgaaca cttggagtg 
      gctcaaaag caacaaaaa tgcaactga cttgaaaga 
      attcttgat caaaagtgt gaccatcaa cgaatctct 
      tattttcga caaaagtgg tgttaattt aataattgg 
      cgaacctgg cattggtaa aacaatttt tgtcaagaa 
      tttaaaaaa acacaattc gccaaatat ttggagcgt 
      tattttgtc cccttctgt gtttcaaaa tcccttcaa 
      tagacacac ttttgagga ggttatttt agcccaaac 
      acacaccaa ccttgacag gaaaattct ccaaatttt 
      gatgaagaa gaaccgagt tgtgtacat tgtggactc 
      ttgggacca aatctcctt ggaacaact cacttgcat 
      ttgtgattt tttccgtac tgaagagtt accactttg 
      ggttacttc acgtcccca cttgcaaaa ctttttcga 
      aacgaagct aaacgtttt gtcacgaac gcttttacc 
      cttcagtga cgtccaaaa aaaatcata catttgcaa 
      acgtctcga agagaaata tccagacat tgggacaga 
      agttctcaa aatcgaaac atacataaa ccaggagat 
      tctaaacaa cccgttaca catctacat gttgaccga 
      agtctttag tcttgatga cgacaagtt ttcgcaact 
      ttcggccga tttgttcaa cgtctgcga cttgtatga 
      gtattgggt tgaacaaaa attcgccct gtattacaa 
      tcaaaagtt tggtgtttg ggccaatga tcgtgtttt 
      cgaagaaca caaaaacta cctcaaacg cgactatca 
      actagctgc gttacatcg aaatgccga atttttgga 
      aaaagtgaa aattgaagg tgatccgat tggtatcat 
      cgaagtaac agacgacca atccccaaa atttttcca 
      cccttcgat tgccgaata tttcatcgc gtcccacct 
      tgctaaaaa ttttgccca agtctcgtc tgactttat 
      tttcaaaga agagaacag aaatgtacg atatttctt 
      cgacttgtt actcgccca aaattccaa accacacat 
      ggcagtatt ttaccgaaa tttggacgt gttgaagaa 
      aatccagaa tttggacaa gtttgttga ttcaggggg 
      acgtaacgt gttacactt ggcgtgttc ttggggcca 
      gacatatcc aaattttga tgtggaaac gcaagaaca 
      agactggat ttatttggt tacaccgaa ggaaacgag 
      tgaagtgtt acgtgaaga ccccaatta caaagaaat 
      gattgttta tctagtaga aaagttgga tttgtggca 
      acgtgacac tttattcaa ctatacggc gttacaata 
      tgctgaagc ttccaactg tctatacac caaaatgat 
      tattttgcg aaaaatgaa caaaacaat agatttgga 
      cacaccgat ggctagtct attgtatta tgcggcgga 
      atttggcta tgaagacgt gttcaattt gtacgaaac 
      gtttccctt gattaaact gaaagacca gtcgtttct 
      gcacctttg tgtgcaaca tgaacactt cctagccca 
      acttttggc aattgaaag ttaccacaa aatgattga 
      ttttgcacc aaatggtga aacagcgtt acacaagtg 
      ttgcaagga aggttacta caagagtgc cttattgtt 
      acgacaaaa tggcgcctc ctgtgttac tactacaag 
      tgatggttg gaccccttt acatttcgc atcactaaa 
      tggacacga atctattgt aacactttt gaaagatgt 
      tgatgtaaa cactgccac taacaaagg ttacactgc 
      gttacactt ggcgtgtta caaaggtta cacgaccat 
      cgtcacgtt actgctaga tttgggggc aaatattta 
      cggtaacac aaatgttac gccattgtt gttggcttg 
      tcagaatgg acaagaaga cgtagcccg ttgtttgtt 
      acaattggg ggcacttgt aactacaag taatagttg 
      gactcctct tcactcagc ttgttacaa ggggcatga 
      aagcattgt caagttatt gatagactt tggggccag 
      tgttaccac agttgatgg taaaaatgt aactccgtt 
      acatttggc atgtcatga agggcatga aacgatcgt 
      ggagttact tctaaattt gagggcaaa tgttgatgc 
      cagagatag tgttggctt tactccgct tcatttggc 
      ttgtcatcg aaattttga acaaattgc caaattgtt 
      gattgcgtt tggtgccag ttgtgatgt ttcggacga 
      tatgggttt gactccgtt acattgggc gtgtcagga 
      agggcgtga tcaaatagt tgagttact tctagcatc 
      tggtgcaaa ccttgaaac tgtgacaaa aacaggcaa 
      aaccccctt tcactttgc atgtttaaa aggacacga 
      agccgtagt tcgcctttt gatgccgtt agttaaccc 
      ccaaattgc aacaactcg aaacttcac ccccttaca 
      cctagcatg ccaagaagg gcacgaaaa cgtagttga 
      attgttact ccaaactgg tgttaacag tgtcacaca 
      agacggctc aacacccct tcattgggc ttcacacaa 
      tggacacta taacatcgt caaaatgtt actccaatc 
      gggggcaaa agttgaaat tagggacag tgaaggctc 
      gacaccact cctcctcgc ctgttatca aggttttga 
      caagattgc caaactttt gatacactt tggtgcaaa 
      tattaccac aagtaacaa tcgtggttt caccccttt 
      gcattgggc aagtcaaca aaatcatcc aaacttggt 
      caaagtttt aatcgaatt gggggcgaa agttacaat 
      agggacgca acaagggtt caccccttt gcatttagc 
      gtgtcagaa agggcacat ttcggttgt taaacgatt 
      gattgtctc gggtgcaaa tattgaaga tgttacaaa 
      taaggggtg gactccact tcattgggc ttctttcaa 
      aggacatga aaccgtcac gaatttatt gctaggggc 
      tgatgctaa tgtcaatat tcctaatgg ggaaggtat 
      gacaccgtt acatttggc ttgttcgaa gggttttgt 
      ccaaattgc aaatacgtt aatcgagtt tggggccag 
      tactgaatg tgagaattg tgatggttt gtctccgct 
      ttatttagc gtgtcaagg ggggcattt agaagtcgt 
      taagttgtt gattatgtt tgggacgat tgttggagg 
      agaaagaag ggcgagaag tagggaaat atcgctgcg 
      ttattttat gatatgaag gaaaaaggt acgaaggtg 
      atgtcaaa
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