Status
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Gene information
General information
TC032608
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC002146
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 20300626..20303027
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC032608
      Gene ontology

      GO terms for Tribolium

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      There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


      GO terms for the homologous Drosophila genes


      Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


      Gene
      {{ goGene }}
      The Tribolium gene the GO term is assigned to.
      GO ID *
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      The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
      Evidence
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      The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
      Reference *
      {{ blurReferenceResult }}
      The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
      Quotation
      If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
      Name
      You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
      Email
      We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
      Publish name
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      * : mandatory fields
      For help on how to submit larger datasets or non experimental data please contact us.
      iBeetle screen iB_03315

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

      adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC032608 RA
        MSGECYAIN VEVGTPLKG NKVMEANCP ELIQDGNKN 
        INQLLEYET MVSSAENKC NQLKSQLER IKQYYSSQS 
        EAKFPLSLD SKKKLSVKA KATKMIRQN SNAISTDRT 
        GAALGTINN ILTGITKSM QVPDLLPKA KISEVQSCS 
        KLEQVFGVD EVTINTKSK QSIQLKPRG KIKSKAKVA 
        SISSSRNFK KMRKATTSL PDKRHTKSS ESQILKVAP 
        AINKRARKV ATKPKVFEA PRKSLVPSI VKHSARNPD 
        LVEIYHNNA VKQTKSSDS DINKKKPKV EPDVEVVPP 
        LNIGENDNQ VDQNKGVQA AKYYMHSFN IKTIPFCLS 
        TSTSPSHNI GINIQQVMS MVKTKQPVG KLSPTLAYN 
        IGLAAGKWE DNPLSILGS TVTTKAFPS QCPLSRSTV 
        NYQHLQEIA KTIPQDIIE ENEEVEDEV SPETRSMII 
        TGPSGDVKV INKKQTIWS ATSEEKRCT CVVPKGTGF 
        QQIVNKFNG ISTKPKNTS ASVIKMKER KSEQIGSGE 
        APLQTDFMP LHNKERKFK EVLGKLHDE FENMTRTYD 
        ELVKRNEEG KATPECLQQ LEKLDNELN SKEEEIVMV 
        MTLYKEVLA LKQQVKTLK ERASKESVS IHSQVPKIG 
        GKNMETAGL LNKLLQRLH YYQSHYKKT
      CDS sequence
      • >TC032608 RA
        atgagtgga gagtgttat gccataaat gtggaagtt 
        ggcacaccg ctaaaaggt aataaagtg atggaagca 
        aattgtccc gagttgatt caagacggc aataaaaat 
        atcaaccaa cttttggaa tatgagaca atggtttcg 
        tcggctgaa aataaatgc aaccagttg aagagccag 
        cttgagcgc atcaaacag tactacagc agtcaatcc 
        gaggccaaa tttccactc tctttagat tcaaaaaag 
        aagctttct gttaaagcc aaagcaaca aaaatgatc 
        agacagaac tccaacgca atcagcacc gacagaacc 
        ggtgctgct ttgggaaca atcaacaac atcttaact 
        ggaattacc aaatcaatg caagttcct gatttgctc 
        ccgaaagca aaaatttcc gaagtccaa agctgcagt 
        aaattggaa caagttttc ggcgttgac gaggttaca 
        atcaacact aaatcgaaa caatcaatt caactcaaa 
        ccaagaggg aaaataaag agcaaagca aaagttgcg 
        tcaattagt tcttcacgg aacttcaaa aaaatgagg 
        aaagcaacg accagtctt cccgataaa cggcacacc 
        aaaagcagc gaaagtcaa atccttaaa gtggcacct 
        gctataaat aagcgggca cgaaaagtg gcaactaaa 
        ccgaaagtt tttgaagca cctagaaag tcactggtt 
        cccagcatt gtcaaacat agtgcgaga aatccggat 
        ttggttgaa atatatcac aataatgct gttaaacaa 
        accaagtca agtgatagt gacattaat aagaagaaa 
        cctaaagtt gagccagat gttgaggtg gttccgccc 
        ttaaatatt ggtgaaaat gataaccaa gttgatcag 
        aacaagggt gtgcaagca gcaaagtac tacatgcac 
        tcgttcaat attaaaact ataccgttt tgtctttcc 
        acttcgaca agtcctagc cacaatatt gggattaat 
        attcaacaa gtgatgagt atggtgaaa actaaacaa 
        ccagtcggg aaattatca ccaactctt gcctataac 
        attggtttg gctgcggga aaatgggaa gataatcct 
        ttatcgatt ttgggttcg actgtgaca actaaagcg 
        tttccatca cagtgtccc ctttctcga agtacggtc 
        aactaccaa caccttcaa gagattgca aaaactata 
        ccacaagac ataattgaa gaaaatgaa gaagttgaa 
        gacgaagtt tcgcctgaa actcgctca atgattatt 
        acaggccca tcgggtgat gtaaaagta atcaataag 
        aaacagact atttggagc gctacttct gaagagaaa 
        agatgcact tgtgtggtc cccaaagga acggggttt 
        caacaaatc gtcaacaaa tttaatgga atttctaca 
        aaacccaaa aacactagt gcgtcagtt attaaaatg 
        aaagaaaga aaatcggaa caaattggt tctggtgaa 
        gcacctctc caaaccgat ttcatgcct ttgcacaac 
        aaggagagg aaatttaag gaagttttg ggtaaatta 
        cacgacgag tttgagaat atgacaagg acttatgat 
        gagttggtg aaaaggaat gaagaaggt aaagcaaca 
        cctgaatgt ctccaacaa ctcgaaaag ttggacaat 
        gaacttaac agtaaagaa gaagaaatc gtgatggtc 
        atgacgttg tacaaagag gtgctggct ttgaagcaa 
        caagtgaaa actctgaaa gaacgtgca agtaaggaa 
        agtgtttcc atacatagc caggttccg aaaattggt 
        ggcaaaaat atggaaact gcgggactc ttgaataaa 
        cttctgcag aggcttcac tattaccag tcgcattac 
        aaaaaaaca taa
      mRNA sequence
      • >TC032608 RA
        ttgcattta caaaatatt aagaaacct ttgaaataa 
        ctgacactg atttatgtc aagcaggat ttttttgta 
        tttttttga actatgttc ttccttcat caaattgtg 
        gcacattaa agttaaaaa atgagtgga gagtgttat 
        gccataaat gtggaagtt ggcacaccg ctaaaaggt 
        aataaagtg atggaagca aattgtccc gagttgatt 
        caagacggc aataaaaat atcaaccaa cttttggaa 
        tatgagaca atggtttcg tcggctgaa aataaatgc 
        aaccagttg aagagccag cttgagcgc atcaaacag 
        tactacagc agtcaatcc gaggccaaa tttccactc 
        tctttagat tcaaaaaag aagctttct gttaaagcc 
        aaagcaaca aaaatgatc agacagaac tccaacgca 
        atcagcacc gacagaacc ggtgctgct ttgggaaca 
        atcaacaac atcttaact ggaattacc aaatcaatg 
        caagttcct gatttgctc ccgaaagca aaaatttcc 
        gaagtccaa agctgcagt aaattggaa caagttttc 
        ggcgttgac gaggttaca atcaacact aaatcgaaa 
        caatcaatt caactcaaa ccaagaggg aaaataaag 
        agcaaagca aaagttgcg tcaattagt tcttcacgg 
        aacttcaaa aaaatgagg aaagcaacg accagtctt 
        cccgataaa cggcacacc aaaagcagc gaaagtcaa 
        atccttaaa gtggcacct gctataaat aagcgggca 
        cgaaaagtg gcaactaaa ccgaaagtt tttgaagca 
        cctagaaag tcactggtt cccagcatt gtcaaacat 
        agtgcgaga aatccggat ttggttgaa atatatcac 
        aataatgct gttaaacaa accaagtca agtgatagt 
        gacattaat aagaagaaa cctaaagtt gagccagat 
        gttgaggtg gttccgccc ttaaatatt ggtgaaaat 
        gataaccaa gttgatcag aacaagggt gtgcaagca 
        gcaaagtac tacatgcac tcgttcaat attaaaact 
        ataccgttt tgtctttcc acttcgaca agtcctagc 
        cacaatatt gggattaat attcaacaa gtgatgagt 
        atggtgaaa actaaacaa ccagtcggg aaattatca 
        ccaactctt gcctataac attggtttg gctgcggga 
        aaatgggaa gataatcct ttatcgatt ttgggttcg 
        actgtgaca actaaagcg tttccatca cagtgtccc 
        ctttctcga agtacggtc aactaccaa caccttcaa 
        gagattgca aaaactata ccacaagac ataattgaa 
        gaaaatgaa gaagttgaa gacgaagtt tcgcctgaa 
        actcgctca atgattatt acaggccca tcgggtgat 
        gtaaaagta atcaataag aaacagact atttggagc 
        gctacttct gaagagaaa agatgcact tgtgtggtc 
        cccaaagga acggggttt caacaaatc gtcaacaaa 
        tttaatgga atttctaca aaacccaaa aacactagt 
        gcgtcagtt attaaaatg aaagaaaga aaatcggaa 
        caaattggt tctggtgaa gcacctctc caaaccgat 
        ttcatgcct ttgcacaac aaggagagg aaatttaag 
        gaagttttg ggtaaatta cacgacgag tttgagaat 
        atgacaagg acttatgat gagttggtg aaaaggaat 
        gaagaaggt aaagcaaca cctgaatgt ctccaacaa 
        ctcgaaaag ttggacaat gaacttaac agtaaagaa 
        gaagaaatc gtgatggtc atgacgttg tacaaagag 
        gtgctggct ttgaagcaa caagtgaaa actctgaaa 
        gaacgtgca agtaaggaa agtgtttcc atacatagc 
        caggttccg aaaattggt ggcaaaaat atggaaact 
        gcgggactc ttgaataaa cttctgcag aggcttcac 
        tattaccag tcgcattac aaaaaaaca taaatttgt 
        aacgagaaa tcgtgtgtc agtgttgta ctcttacaa 
        cagataaat aaagaatgg caaaaaac
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY