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Gene information
General information
TC002139 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid22: 332000..346462
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002139
    iBeetle screen iB_03314
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 80.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002139 RA
      MRKKNRSAP KTVTDFDPE SESFQRKKW FEEIAEDPK 
      DFKPVTEQI LIELKEEAK KCHDAEVAN YNIKNSKSN 
      SDYKWMKTV MSKGTVSDK IAASVVAIQ DNPVCSLDT 
      LQNLVNMVK VGKKKECVT TIDTLTELF LSDLLRPDQ 
      KLKAFHQRP LALLQELSS GNAITRRKL LSNWYFEDQ 
      LKELYTTFV LALNNVAHD VLDNNKEKA ISSMYKLLA 
      GNPEQEKNL LTYLVNKLG DPSQKVASK AIYSLGQLL 
      FKHPNMQGV VLSEVEKLL FRSNISPKA QYYGLCFLS 
      QFYLSHESN EVARRLIEV YFSFFKACV KKGEIDSRM 
      MSALLMGVN RAYPYAKLE MEKISEHID TMYRVVHIA 
      NFNISLHAL SLLYQVSDH HNNVTDRFY SALYKKLID 
      PKLFTTTHQ AMLLSLIYK ALLKDTETT RIKVFVKLI 
      FIDASAFAC GVLYLISQL MGKKQNIQS LVLKQTVLK 
      NDGSDDEDE ERYHDVKDD EIEIKQEDE EGSGDDIKP 
      DVSVLNHAI KPSWDHCAN SSKKEKSPT YNALSRNPL 
      YAGGDFCAY TELFDLKNH FHPTVALYA TNILDGNII 
      KYSGDPLKD FTLIRFLDR FVFKNPKKY EEGTVQGAH 
      PTFGKRKLY LPKGLRSLR VNSANYLNE DSKNIPVDE 
      MFMYSYLRK KYHDREQDF GDKSDEDDS DLESVQSEE 
      FEEMLEKMA GLPKDDEED LDFLNDVGD NLKTNGKSQ 
      KDKKRTDDD DSDDDDSEM EDASDEELS MDEDEDFDE 
      DDLEMNDDD KMLLGDLDD DEDEEVVFD DEEPKSAKS 
      KKKKSKDTN SIFASAEEF ASLLDDEGS SKIAPGSSN 
      ALSNKDNAS TKQLAWEQS RNRWLKGFD EVVGKKKNS 
      KGSNFGKKR KGPNKFNNK AKKRKN
    CDS sequence
    • >TC002139 RA
      atgagaaag aaaaaccgt agtgcacct aaaaccgtg 
      acggacttt gatcctgaa tcagagtcg tttcaacgg 
      aaaaaatgg ttcgaagag attgccgaa gaccctaaa 
      gactttaaa cctgtaact gagcaaatt cttatagaa 
      ctgaaggaa gaggcgaaa aagtgtcac gatgcagaa 
      gtagcaaat tataacatc aaaaattcc aaaagtaat 
      tccgactac aaatggatg aagacggtc atgtctaaa 
      ggcacagtt tctgataaa atcgcagcc tcggtggtt 
      gcaattcag gacaatcct gtttgtagt ttagacacg 
      ttacaaaat cttgtgaac atggttaaa gttggaaaa 
      aagaaagag tgtgtcaca actattgac actcttacc 
      gaattgttt ttgtccgat ttattgcga cctgatcaa 
      aaattgaaa gcgtttcat caacgcccc cttgctctt 
      ttgcaagag ttgtcatca gggaatgct ataactcgt 
      aggaagttg ctcagtaat tggtatttt gaggaccag 
      ttaaaggag ttgtacact acttttgtc ctggctttg 
      aataatgta gctcatgat gtgcttgat aacaacaag 
      gagaaggcc ataagttcg atgtataaa cttttggca 
      ggaaatccg gagcaagaa aagaatttg ttaacatac 
      ctcgttaat aaactgggc gacccttca caaaaagtg 
      gcctcaaaa gccatttac agcttagga cagctacta 
      ttcaaacat cccaacatg caaggagtt gttttaagc 
      gaagttgag aaattatta ttccgttca aacatttca 
      ccgaaagca cagtattac ggtttatgt tttttgtca 
      cagttttat ttaagtcat gaatcaaac gaagtcgca 
      agaagatta atagaagtc tatttttcc ttttttaaa 
      gcttgcgtg aaaaagggt gaaattgat agtcgaatg 
      atgtcggct ttgttaatg ggggttaat agggcgtat 
      ccttatgcc aaactagaa atggaaaaa atttcagaa 
      catattgat acaatgtac agggtggtg catattgca 
      aattttaat attagtctt catgctctt tctttactg 
      tatcaagtc tccgatcat cacaataat gtaacagat 
      cggttttac tcggcactt tataaaaaa ctaatcgac 
      ccaaaatta tttacaaca acgcatcaa gcgatgttg 
      ttgagttta atttacaaa gctcttttg aaagacacc 
      gaaacgaca cgtattaag gtttttgtt aagcttatc 
      tttattgat gcaagtgct tttgcctgt ggtgtattg 
      tatcttatt tcccaatta atgggcaaa aaacaaaac 
      attcaatcg cttgttctt aaacaaaca gtgttgaaa 
      aatgacggg tctgacgac gaggacgag gaaagatat 
      cacgatgtc aaggacgat gaaattgaa attaaacaa 
      gaagatgaa gagggaagt ggtgacgac ataaaaccc 
      gacgtttct gttttaaat catgcgatt aaaccaagt 
      tgggatcat tgtgctaac tcatccaag aaggaaaaa 
      tcaccgact tacaatgct ttaagtcgt aacccgctt 
      tatgcaggt ggcgatttt tgcgcctac actgaatta 
      tttgaccta aaaaatcat tttcatcca actgttgca 
      ctatacgcc acaaacata ttggatgga aatataatt 
      aaatattca ggggatccc ttaaaagat tttacactg 
      atcagattt ttggatagg tttgttttc aaaaatccg 
      aaaaaatac gaagagggt acagttcaa ggagcgcat 
      ccaacgttt ggtaaaaga aaattgtat cttcctaaa 
      ggtttaagg tcattgcgt gtaaattca gcaaattat 
      ttgaatgaa gattcgaaa aatattcct gtagatgag 
      atgtttatg tatagttat ttacggaag aagtaccac 
      gacagagag caagatttt ggtgataaa agcgatgag 
      gatgatagt gatttggag tcggttcaa agtgaggaa 
      tttgaagaa atgttggag aaaatggct ggtcttcct 
      aaggatgat gaggaagat ttggacttt ttgaatgat 
      gttggagat aatcttaag actaacggg aaaagccaa 
      aaggataaa aaacggaca gatgatgat gatagtgat 
      gacgatgat agcgagatg gaggatgct tctgacgaa 
      gaattgtca atggacgaa gatgaagat ttcgatgaa 
      gatgacctt gaaatgaac gatgatgac aaaatgctt 
      ctgggtgac ttagacgac gatgaagac gaggaagtt 
      gtttttgac gatgaagaa ccgaagtca gcaaaatca 
      aagaagaaa aaatccaaa gacacaaac tcaatattt 
      gcatcagct gaagaattt gcctcattg cttgacgat 
      gaaggtagt tcgaaaata gccccagga agttcaaat 
      gcgttatca aataaggat aatgcgagc actaaacaa 
      cttgcctgg gaacagagt agaaatagg tggttgaag 
      ggatttgat gaagtagtt ggcaaaaag aaaaatagt 
      aaaggtagc aattttggc aagaaaagg aaaggtcca 
      aataaattt aataataag gcaaagaag aggaagaat 
      taa
    mRNA sequence
    • >TC002139 RA
      ggttatgta ttactttat tgttgctac agattatga 
      gataatttt gtgcttgat tatttcaga acagcctat 
      agcttgtgc ctcatgtca actgtcaac acgtgctga 
      cataaccca acttttgaa agtgcgttt taatttttg 
      aattgtgtt ttttcatac agtttttgc gaataatga 
      gaaagaaaa accgtagtg cacctaaaa ccgtgacgg 
      actttgatc ctgaatcag agtcgtttc aacggaaaa 
      aatggttcg aagagattg ccgaagacc ctaaagact 
      ttaaacctg taactgagc aaattctta tagaactga 
      aggaagagg cgaaaaagt gtcacgatg cagaagtag 
      caaattata acatcaaaa attccaaaa gtaattccg 
      actacaaat ggatgaaga cggtcatgt ctaaaggca 
      cagtttctg ataaaatcg cagcctcgg tggttgcaa 
      ttcaggaca atcctgttt gtagtttag acacgttac 
      aaaatcttg tgaacatgg ttaaagttg gaaaaaaga 
      aagagtgtg tcacaacta ttgacactc ttaccgaat 
      tgtttttgt ccgatttat tgcgacctg atcaaaaat 
      tgaaagcgt ttcatcaac gcccccttg ctcttttgc 
      aagagttgt catcaggga atgctataa ctcgtagga 
      agttgctca gtaattggt attttgagg accagttaa 
      aggagttgt acactactt ttgtcctgg ctttgaata 
      atgtagctc atgatgtgc ttgataaca acaaggaga 
      aggccataa gttcgatgt ataaacttt tggcaggaa 
      atccggagc aagaaaaga atttgttaa catacctcg 
      ttaataaac tgggcgacc cttcacaaa aagtggcct 
      caaaagcca tttacagct taggacagc tactattca 
      aacatccca acatgcaag gagttgttt taagcgaag 
      ttgagaaat tattattcc gttcaaaca tttcaccga 
      aagcacagt attacggtt tatgttttt tgtcacagt 
      tttatttaa gtcatgaat caaacgaag tcgcaagaa 
      gattaatag aagtctatt tttcctttt ttaaagctt 
      gcgtgaaaa agggtgaaa ttgatagtc gaatgatgt 
      cggctttgt taatggggg ttaataggg cgtatcctt 
      atgccaaac tagaaatgg aaaaaattt cagaacata 
      ttgatacaa tgtacaggg tggtgcata ttgcaaatt 
      ttaatatta gtcttcatg ctctttctt tactgtatc 
      aagtctccg atcatcaca ataatgtaa cagatcggt 
      tttactcgg cactttata aaaaactaa tcgacccaa 
      aattattta caacaacgc atcaagcga tgttgttga 
      gtttaattt acaaagctc ttttgaaag acaccgaaa 
      cgacacgta ttaaggttt ttgttaagc ttatcttta 
      ttgatgcaa gtgcttttg cctgtggtg tattgtatc 
      ttatttccc aattaatgg gcaaaaaac aaaacattc 
      aatcgcttg ttcttaaac aaacagtgt tgaaaaatg 
      acgggtctg acgacgagg acgaggaaa gatatcacg 
      atgtcaagg acgatgaaa ttgaaatta aacaagaag 
      atgaagagg gaagtggtg acgacataa aacccgacg 
      tttctgttt taaatcatg cgattaaac caagttggg 
      atcattgtg ctaactcat ccaagaagg aaaaatcac 
      cgacttaca atgctttaa gtcgtaacc cgctttatg 
      caggtggcg atttttgcg cctacactg aattatttg 
      acctaaaaa atcattttc atccaactg ttgcactat 
      acgccacaa acatattgg atggaaata taattaaat 
      attcagggg atcccttaa aagatttta cactgatca 
      gatttttgg ataggtttg ttttcaaaa atccgaaaa 
      aatacgaag agggtacag ttcaaggag cgcatccaa 
      cgtttggta aaagaaaat tgtatcttc ctaaaggtt 
      taaggtcat tgcgtgtaa attcagcaa attatttga 
      atgaagatt cgaaaaata ttcctgtag atgagatgt 
      ttatgtata gttatttac ggaagaagt accacgaca 
      gagagcaag attttggtg ataaaagcg atgaggatg 
      atagtgatt tggagtcgg ttcaaagtg aggaatttg 
      aagaaatgt tggagaaaa tggctggtc ttcctaagg 
      atgatgagg aagatttgg actttttga atgatgttg 
      gagataatc ttaagacta acgggaaaa gccaaaagg 
      ataaaaaac ggacagatg atgatgata gtgatgacg 
      atgatagcg agatggagg atgcttctg acgaagaat 
      tgtcaatgg acgaagatg aagatttcg atgaagatg 
      accttgaaa tgaacgatg atgacaaaa tgcttctgg 
      gtgacttag acgacgatg aagacgagg aagttgttt 
      ttgacgatg aagaaccga agtcagcaa aatcaaaga 
      agaaaaaat ccaaagaca caaactcaa tatttgcat 
      cagctgaag aatttgcct cattgcttg acgatgaag 
      gtagttcga aaatagccc caggaagtt caaatgcgt 
      tatcaaata aggataatg cgagcacta aacaacttg 
      cctgggaac agagtagaa ataggtggt tgaagggat 
      ttgatgaag tagttggca aaaagaaaa atagtaaag 
      gtagcaatt ttggcaaga aaaggaaag gtccaaata 
      aatttaata ataaggcaa agaagagga agaattaat 
      aatcgacaa ggaagcagt cgattgcat tttaattaa 
      tataattct ttacgaatc