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Gene information
General information
TC002138 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid22: 285689..327918
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002138
    iBeetle screen iB_03313
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002138 RA
      MEKPWGVLG LFTGLLLCA LCNGLPINL ENTVADSHT 
      QLLTGLVAV LLTVCSVVF LAGCLCCQR RKGFKEFRD 
      SPVVASAAS NLDHGHVNP VANGEFTIF TPLSPPHQN 
      NNVFLANQQ IITRTQEDY IFGDVDVSS WFNGTETDF 
      PRIKLKYIK EIGKGWFGR VVEGAAQDI DSEQKWTPV 
      VVRILEATA SKRERVVFL HDASIYRCG SHPNILKLI 
      GRCLDTIPL LLLQEYCPQ GDLKNYLRQ NKPTADKLL 
      STEYPLVWC CQLSSALKF LHENRLSHP DLAARNCQL 
      TSNLTLKLG DYGLAVLQY PEDYYLGAA GVSVRWCAP 
      ESLSYTSTT IEPKTVTLE ANIWSFAVT MWEICECGE 
      QPYSSLTDD EVISQVLGS QKVRLSRPR FQMLYNDYI 
      FRLMQLCWT TSESRPAMS QIDLMLGDL LQVYKNTNL 
      TKLEPPISM NDFDKRWES FKPNSIVKT DNQITDNAD 
      LDLDDSQIV TSKPLSPSL NNLHGSLDN LLTSNVQME 
      SWLENVATR TGDMSYVRG LSDAIKDLD NAIALENST 
      SSESSPKIQ FRLGPFGDK PQIAESPHE SLNDSVQRP 
      SSGSETEEE NWKKKIERG AYSEKVRQK SRSVADLMV 
      LTHIDQSES ESETPLPSL DYRTNYRNV RYAPKQNLE 
      SCGLMFGSE GNLLSVQET FQEELKKLQ EERRDSLLF 
      VPENSSQNS DSVSCIAGS SSGFFLQEL SEIKPANQV 
      FNVFNVTID KYSPVRLNL DELINFKED QFEVPKLAD 
      IIKSNSELV DHLKNSDFG SEVMYDSGV EGDKVESTE 
      SLRNIIFEP EHITAKPHD GAVYQVQKL VDLIQDNDE 
      LMDYIIARY ETESVGRMT DSLDSLETP SNELIAKND 
      CINTTKEFL SREINSHSD IEEPKVAFD LSDSQDEAM 
      PNSLTHSTP FGKKHELTS RDTFSSLNL FQDEKINDS 
      SNLYSLETW DNFLGKALD SQSESQDEM FDSFSSEPQ 
      SLLFIANES EQGDKNATF VKEKAVDVD DINVELETD 
      VNGGGWFLH PQSGDVSGE MVVPTNAGG DSYVGFNVD 
      DEIMAAIRT ELLNKLPQA QGGSNGVVQ DEEEWDSVE 
      RNEVFLRYN VYNTPLSPI PEESLLEDN SYTPRQQES 
      DDEDSDWSD QCEREVLPH ELESHSAQT LESPVHPER 
      LHRHTPSQD SCCSNDTLF NLEELTCGV NDSERDNNS 
      PTPRISECE SQTGEEQQV ANNAMIHDI NNDSNIGAL 
      NDKTTTTEK DSDNSASGC RTSETVKES VEMKQTIEF 
      LAREREHDC SKTSEESNS ESTTDHLLP FVTKQVAPL 
      PSPEDNPWK QLPASLLSY DKVMLLPSP EAVEKNNTE 
      ENLYVNVPQ NNCESSEFE NVENLDDYY NEADYVNIV 
      NLENSKNSA EFINNKQIN VVIEDASER SSPDYVNND 
      LIINDDDDN EHDIFGVLT DIRFSGPSD SQMMSTSFS 
      ESNDLNDEQ DWDSGSDTR SSSSGEFIW KEGEHEESL 
      KALRAAPQD VLEDIHPME GIQEETSSE SSGSDDDGE 
      SPEFVPSAW DKYAIPAKS ALRSPEKTL EKTDKKKAK 
      GVWFKKQKY HCVYEYPRE PESPVLHSF DLWKPQPDY 
      SSFDDWELD GTDPCLPAD NQDDCDFTQ NYFQAKPNR 
      NLNLYQLSS ITDFNSSDP GLDDEFFIS SSAKPFDMI 
      SGLSSQFFP GNWTDLSKT TENATPDSG VEDITPAGF 
      TEGEFTAQF RTVKPLKQL ASEAVKRSK KVEETRNHD 
      MLGGLRHTR NRLKLDLPP SPSAFTSSK MFSIEPVID 
      PVINREKPT FTTFGKSRF LVQHVDTPP DDEVSKSKN 
      VSFEALPYK PIQKHIIKE DFETSREKG KVESVRGEA 
      SLLDSADED SGIESSTLE RKLTNPV
    CDS sequence
    • >TC002138 RA
      atggaaaaa ccatgggga gttttgggt ctgttcact 
      ggactgctg ctgtgtgca ttatgcaat gggctgccc 
      attaatctg gagaacacg gtagcagat tctcacacc 
      caattgctg acaggactt gtggcagtt ttattaact 
      gtttgcagc gtggtgttc ctggcaggc tgcttgtgc 
      tgtcaacga aggaaagga tttaaggaa tttcgggac 
      agtcctgtt gtagcctct gcagcttcg aatttagac 
      catggtcat gttaatcct gttgcaaac ggtgaattc 
      actattttc accccttta tcgccccca caccaaaat 
      aataacgta tttcttgcc aatcaacaa ataattaca 
      cgtactcag gaggattac attttcgga gatgtggat 
      gttagttcc tggtttaat ggcaccgaa accgatttt 
      ccgcgaatt aaactaaag tacataaaa gaaattggc 
      aaaggctgg tttggaaga gtcgttgaa ggcgcagcc 
      caagacatc gatagcgag caaaaatgg acacctgta 
      gtggttcgc attttggaa gccacagct tcgaagaga 
      gaacgtgtc gtgttttta cacgacgcg tccatttat 
      cgatgtggt tcacatcca aatatttta aaattaatt 
      gggagatgt ttggatacg atcccactt ttgctattg 
      caagaatat tgcccgcaa ggtgatctt aaaaattac 
      ctaaggcaa aataaaccg accgctgat aagttacta 
      tcaacggaa tatcccctg gtttggtgt tgccaactg 
      agttcggct ctaaaattc ctccatgaa aatagattg 
      agtcatccg gatttagcg gcgagaaat tgtcagctc 
      acttcaaat ctaacgtta aaattgggt gattatggc 
      ctagctgtt ctccaatat ccggaggat tattatttg 
      ggagcggcg ggtgtttca gtgagatgg tgtgcacca 
      gaatcgtta tcttacact tccacgaca attgagccc 
      aagactgtt acactggaa gctaatatt tggtcattt 
      gctgttaca atgtgggaa atttgcgaa tgtggggaa 
      caaccttat tcttctttg actgatgat gaggtcatt 
      tcgcaagtt ttggggtca cagaaagtc agattaagc 
      aggcctagg tttcaaatg ctctacaat gattacatt 
      ttccgctta atgcaactt tgttggaca accagcgaa 
      tcacgtcca gccatgtcc cagatcgac ctaatgctg 
      ggcgacctc ctccaagtc tacaaaaac acgaactta 
      accaaactt gagccaccc atttcaatg aacgatttc 
      gacaagcgt tgggagtcg tttaaaccg aacagcatc 
      gtcaagact gacaaccaa attacggac aatgctgat 
      ctggacttg gacgattcc caaatcgtc acttcaaaa 
      cccctatca cccagctta aacaacttg cacggttcc 
      ctcgacaac ttactcact tcgaacgtg caaatggaa 
      tcatggcta gaaaacgtc gccactcga accggcgac 
      atgtcatac gttcggggc ctttcggac gccatcaag 
      gacctggac aacgctata gcgctggaa aacagcact 
      agttccgaa tcgagtccg aaaatccag ttcagactt 
      ggccccttc ggggacaag ccccaaata gccgaatca 
      ccccacgag agtttgaac gatagtgtc caaaggccc 
      agcagtggt agcgagacc gaggaggaa aactggaag 
      aagaaaatc gaacggggg gcttattcg gaaaaagtg 
      cgtcagaaa tcgcgttcg gtcgccgat ttgatggtc 
      ttgacgcac atcgaccaa tcagagtcc gagtccgaa 
      acacctttg cctagttta gactatcga acgaattat 
      agaaatgtt cgatacgcg cctaagcaa aatctagag 
      agttgtggt ttgatgttc ggtagtgaa ggtaatctc 
      ctaagtgtg caagaaacg ttccaggaa gaattgaag 
      aaattgcaa gaggaacga agagatagt ctgcttttt 
      gtacctgaa aacagtagt caaaatagt gatagtgtc 
      tcgtgtata gcggggagt tcgagcggt ttttttctg 
      caagagttg agcgagatc aagcctgcg aatcaagtg 
      ttcaatgtg ttcaatgtt accatagat aagtacagt 
      ccggtgcgc ttgaatctg gacgaactt atcaatttc 
      aaagaggac cagttcgag gtcccaaaa ctggccgat 
      attatcaaa agcaatagt gaacttgtc gaccatttg 
      aagaattcc gattttgga agcgaggtc atgtacgat 
      tcgggcgtt gaaggcgat aaagttgag tcaactgaa 
      tcactcaga aatatcatt tttgaaccc gaacatatc 
      acggcaaag ccacacgat ggcgctgtt tatcaagtg 
      cagaagctg gtcgatctg atccaagat aacgatgag 
      ttaatggac tatattatc gcacggtac gagaccgaa 
      agtgtcggt cggatgacc gacagttta gacagtctt 
      gagacgcct tccaatgaa ctaatcgcg aaaaatgat 
      tgtattaac acaaccaaa gagtttcta agccgggag 
      atcaacagc cacagcgat atagaagag ccaaaggtg 
      gcgttcgac ctaagtgac agtcaagat gaagcaatg 
      cctaacagt ttaactcac tcaacaccg tttgggaaa 
      aaacacgag ttaacttcc cgtgacacg ttctctagc 
      ttgaatctg ttccaagac gagaaaatc aacgattct 
      tcaaatctc tactcgtta gaaacgtgg gataatttc 
      cttggaaaa gcacttgac agtcagagt gaatcccaa 
      gacgaaatg tttgatagt ttttcgtct gagccgcaa 
      agtttgttg tttatcgca aacgagtcg gagcaaggc 
      gataaaaac gcgacgttt gtgaaggag aaggctgtt 
      gatgttgat gatattaat gtggagttg gagacggat 
      gtgaatggt ggaggttgg ttccttcat ccacagtct 
      ggggatgtt tctggggag atggtggtg cccacgaat 
      gcgggtggt gatagttat gttgggttt aatgtggat 
      gatgaaatc atggcggcg attaggacc gagcttttg 
      aataagttg ccgcaggct cagggtggt tccaatggg 
      gttgttcag gatgaggag gagtgggat tcggtggag 
      aggaatgag gtgtttttg aggtataat gtttacaat 
      acgccgtta tcgccaatt ccggaggaa agtctgctt 
      gaggataat tcttataca ccaaggcaa caggaaagc 
      gatgacgaa gacagcgat tggtcggac caatgcgaa 
      cgcgaagtt ttaccacac gagctggaa agtcactca 
      gcccaaact ctcgaaagt ccagttcat cccgaacgt 
      ctccacaga cacacaccc agccaggat tcctgttgt 
      tcaaacgac actctcttc aacttggaa gagctcaca 
      tgtggcgtt aatgactca gaaagggac aataattcc 
      ccaacccct cgaatctcc gaatgtgaa tcacaaacc 
      ggcgaggaa caacaggta gcaaacaac gcgatgatt 
      catgacata aacaatgac tcaaacatt ggagcctta 
      aatgacaaa actacaaca acggagaaa gatagtgat 
      aattcggca agtgggtgc agaacaagt gagacagtg 
      aaagaaagt gttgagatg aaacaaacg attgagttt 
      ctggcacgt gaaagagaa cacgattgt tccaaaacc 
      agcgaagaa tccaattcg gaatcgaca actgatcat 
      cttttaccg tttgttact aagcaggtg gcaccactg 
      ccatcgcct gaggacaac ccctggaaa caattaccg 
      gcgtctttg ttgagttac gataaggta atgttgtta 
      ccatcgcct gaagccgtt gaaaaaaac aacacggaa 
      gaaaatctc tacgttaac gttccgcaa aataattgc 
      gaatcgagc gaattcgaa aatgtggag aatttggat 
      gattattac aacgaggct gattatgtg aatatagtg 
      aatttagaa aatagcaaa aactcggct gaatttatc 
      aacaataaa caaattaac gtcgtgatt gaagatgca 
      agtgagcgg agtagtccc gactatgtt aataatgat 
      ctgattatt aatgatgat gatgataat gaacatgat 
      atttttggg gttttaacc gatattagg tttagtgga 
      ccaagtgat agtcaaatg atgtcgaca tcatttagc 
      gaaagtaat gatttaaat gatgagcaa gattgggat 
      agtggttcg gatacgagg tctagttca agtggagag 
      tttatctgg aaggagggt gagcacgaa gagtctctc 
      aaggcctta cgcgctgcc ccacaagat gtgttggaa 
      gatattcac cccatggaa ggcatacaa gaagaaact 
      tcaagtgag agtagcggg agtgatgat gatggtgaa 
      agtcccgaa ttcgtccca tcagcttgg gacaagtat 
      gcaatacct gccaaatct gcactgaga tctccagag 
      aaaacactc gaaaagact gataaaaaa aaagcaaaa 
      ggtgtgtgg tttaaaaaa caaaaatat cattgtgtt 
      tatgaatat ccgagagaa cccgaaagt ccagtactc 
      cacagtttt gatttgtgg aagccacag cccgattat 
      agttccttt gacgactgg gaactagac ggcactgac 
      ccttgcctc cctgccgat aaccaagac gattgcgat 
      tttacccaa aattacttt caagccaaa cctaatcga 
      aacctaaat ttgtaccaa ctgagtagc ataacagac 
      tttaattca agcgaccca ggcctggac gacgaattt 
      ttcataagt agctcagcc aaacccttc gacatgatc 
      agtggctta agttcccag ttcttcccc ggaaattgg 
      accgatttg agcaaaacc accgaaaat gcgaccccg 
      gacagtggc gtggaagat atcacaccg gccggtttt 
      acagagggc gaatttacc gcccagttt agaaccgta 
      aaaccactg aagcaacta gcttccgaa gcagtgaaa 
      cgaagcaaa aaggttgaa gaaacaaga aatcatgac 
      atgttgggc ggtttgaga cacacgaga aatcggcta 
      aagcttgat ttaccgccg agtccaagc gcgttcact 
      tcgagtaaa atgtttagt attgaaccg gtcattgac 
      ccggttatt aaccgggaa aaaccgacg tttacaacg 
      ttcgggaaa agtcggttt ttggtacaa catgtcgat 
      acgccaccg gatgacgaa gtgagcaaa agtaaaaac 
      gtttcgttt gaagcgtta ccttataaa ccaatccag 
      aaacatata attaaggaa gatttcgaa acgagtcga 
      gagaaaggt aaggtggaa agtgtgcgt ggcgaagcc 
      agcctacta gatagtgcc gatgaagac agtgggatt 
      gagtcaagc actctggaa cgaaaactg actaaccct 
      gtttag
    mRNA sequence
    • >TC002138 RA
      tttttattt taatttctc attgtttat atacagttt 
      tctttttta caaattgtt aactatttc aatttaaat 
      caatcgtat acgcaacgt tttgaaaac ggccggttt 
      atagaaagc tcaattaaa atttaatgc gttgttaat 
      agttaattt tgaagcaag aatgaaaga agggggttt 
      gagtttctt tataaaaaa attagatac taataaatt 
      gctgagtaa gtaatagtc attttctaa gtaaaatat 
      tgtaaatgc taggtaaaa taatctaat tgtttggca 
      gaaatgctt ggtaaaata ttttaaatg ctggtaagt 
      ggtatgaag atttttaaa taagatttg ctaagaaaa 
      aaaaaatac ggcttataa aatggtata ccttcttag 
      atatttcac acccacttt gctgagcta aatatttaa 
      tatgctaga tatggacta attgaacca aaaaatcca 
      tctttttaa aaaagattt tttaagtgt tggaatgga 
      caaatgtag tgacatatt tagtaggag gaagtaaaa 
      agtgtttga ctcttgtta taggcaaaa ggatttgca 
      ttgatacat ttttttcct ttaaatata cgaattccg 
      aattacaga agtgtattt ttgtataca atatttgat 
      aaataaagt tatcaagtt aattgatgt tttatttta 
      ttaaattac aaacgatta gttacatta taaagaaaa 
      ttatttctt cttcccctt acttgtgga cccctgttt 
      tgccttcct ctcctgctg catttgctt cagtttcat 
      attgagttt agtttgtgc ttgcgaatg cttctcttg 
      cgtcaactt cacatttgg gtgcaagta cttctgcct 
      ctcgtcttt cctcacagc atccccaca cccgtgtat 
      ttcggggag cccatgcgt ggggcaaaa cctcccttt 
      gcagtactt gcactcgat ggcgaagtc attggtccg 
      atttttgca ttttatgaa cgaacatgt gttatcaat 
      tttcttgac cttgtctag gacttggtc taggtcgtg 
      cgacgttgt ggcttcctt caaatcctt ttccttgtt 
      ctttacttt ggaattttc cgtgattgt tattgttgc 
      cattttgag gttagacaa ctgtcactt agtgtcaaa 
      aatgggtta ttgcctgca gggggcgct gccgtgcta 
      gcgcaaaaa atttcgact ggagctcga gtgaaatgt 
      catttcgac gcaaaaatg gttgaatga tgttggaga 
      gctgagtta tttgttaaa aattgcgag ttttctgcg 
      aaaacacca gtgattaag tgcgtaatt gtgtgtttt 
      aagatgtga cgtgtgagt tgggttgat aactggtga 
      ctgggtgca gtgcatgag acaaccgtt gactggtct 
      ttggtgaat gatgttttt gtttttatg aaagattgc 
      aaattatgg aaaaaccat ggggagttt tgggtctgt 
      tcactggac tgctgctgt gtgcattat gcaatgggc 
      tgcccatta atctggaga acacggtag cagattctc 
      acacccaat tgctgacag gacttgtgg cagttttat 
      taactgttt gcagcgtgg tgttcctgg caggctgct 
      tgtgctgtc aacgaagga aaggattta aggaatttc 
      gggacagtc ctgttgtag cctctgcag cttcgaatt 
      tagaccatg gtcatgtta atcctgttg caaacggtg 
      aattcacta ttttcaccc ctttatcgc ccccacacc 
      aaaataata acgtatttc ttgccaatc aacaaataa 
      ttacacgta ctcaggagg attacattt tcggagatg 
      tggatgtta gttcctggt ttaatggca ccgaaaccg 
      attttccgc gaattaaac taaagtaca taaaagaaa 
      ttggcaaag gctggtttg gaagagtcg ttgaaggcg 
      cagcccaag acatcgata gcgagcaaa aatggacac 
      ctgtagtgg ttcgcattt tggaagcca cagcttcga 
      agagagaac gtgtcgtgt ttttacacg acgcgtcca 
      tttatcgat gtggttcac atccaaata ttttaaaat 
      taattggga gatgtttgg atacgatcc cacttttgc 
      tattgcaag aatattgcc cgcaaggtg atcttaaaa 
      attacctaa ggcaaaata aaccgaccg ctgataagt 
      tactatcaa cggaatatc ccctggttt ggtgttgcc 
      aactgagtt cggctctaa aattcctcc atgaaaata 
      gattgagtc atccggatt tagcggcga gaaattgtc 
      agctcactt caaatctaa cgttaaaat tgggtgatt 
      atggcctag ctgttctcc aatatccgg aggattatt 
      atttgggag cggcgggtg tttcagtga gatggtgtg 
      caccagaat cgttatctt acacttcca cgacaattg 
      agcccaaga ctgttacac tggaagcta atatttggt 
      catttgctg ttacaatgt gggaaattt gcgaatgtg 
      gggaacaac cttattctt ctttgactg atgatgagg 
      tcatttcgc aagttttgg ggtcacaga aagtcagat 
      taagcaggc ctaggtttc aaatgctct acaatgatt 
      acattttcc gcttaatgc aactttgtt ggacaacca 
      gcgaatcac gtccagcca tgtcccaga tcgacctaa 
      tgctgggcg acctcctcc aagtctaca aaaacacga 
      acttaacca aacttgagc cacccattt caatgaacg 
      atttcgaca agcgttggg agtcgttta aaccgaaca 
      gcatcgtca agactgaca accaaatta cggacaatg 
      ctgatctgg acttggacg attcccaaa tcgtcactt 
      caaaacccc tatcaccca gcttaaaca acttgcacg 
      gttccctcg acaacttac tcacttcga acgtgcaaa 
      tggaatcat ggctagaaa acgtcgcca ctcgaaccg 
      gcgacatgt catacgttc ggggccttt cggacgcca 
      tcaaggacc tggacaacg ctatagcgc tggaaaaca 
      gcactagtt ccgaatcga gtccgaaaa tccagttca 
      gacttggcc ccttcgggg acaagcccc aaatagccg 
      aatcacccc acgagagtt tgaacgata gtgtccaaa 
      ggcccagca gtggtagcg agaccgagg aggaaaact 
      ggaagaaga aaatcgaac ggggggctt attcggaaa 
      aagtgcgtc agaaatcgc gttcggtcg ccgatttga 
      tggtcttga cgcacatcg accaatcag agtccgagt 
      ccgaaacac ctttgccta gtttagact atcgaacga 
      attatagaa atgttcgat acgcgccta agcaaaatc 
      tagagagtt gtggtttga tgttcggta gtgaaggta 
      atctcctaa gtgtgcaag aaacgttcc aggaagaat 
      tgaagaaat tgcaagagg aacgaagag atagtctgc 
      tttttgtac ctgaaaaca gtagtcaaa atagtgata 
      gtgtctcgt gtatagcgg ggagttcga gcggttttt 
      ttctgcaag agttgagcg agatcaagc ctgcgaatc 
      aagtgttca atgtgttca atgttacca tagataagt 
      acagtccgg tgcgcttga atctggacg aacttatca 
      atttcaaag aggaccagt tcgaggtcc caaaactgg 
      ccgatatta tcaaaagca atagtgaac ttgtcgacc 
      atttgaaga attccgatt ttggaagcg aggtcatgt 
      acgattcgg gcgttgaag gcgataaag ttgagtcaa 
      ctgaatcac tcagaaata tcatttttg aacccgaac 
      atatcacgg caaagccac acgatggcg ctgtttatc 
      aagtgcaga agctggtcg atctgatcc aagataacg 
      atgagttaa tggactata ttatcgcac ggtacgaga 
      ccgaaagtg tcggtcgga tgaccgaca gtttagaca 
      gtcttgaga cgccttcca atgaactaa tcgcgaaaa 
      atgattgta ttaacacaa ccaaagagt ttctaagcc 
      gggagatca acagccaca gcgatatag aagagccaa 
      aggtggcgt tcgacctaa gtgacagtc aagatgaag 
      caatgccta acagtttaa ctcactcaa caccgtttg 
      ggaaaaaac acgagttaa cttcccgtg acacgttct 
      ctagcttga atctgttcc aagacgaga aaatcaacg 
      attcttcaa atctctact cgttagaaa cgtgggata 
      atttccttg gaaaagcac ttgacagtc agagtgaat 
      cccaagacg aaatgtttg atagttttt cgtctgagc 
      cgcaaagtt tgttgttta tcgcaaacg agtcggagc 
      aaggcgata aaaacgcga cgtttgtga aggagaagg 
      ctgttgatg ttgatgata ttaatgtgg agttggaga 
      cggatgtga atggtggag gttggttcc ttcatccac 
      agtctgggg atgtttctg gggagatgg tggtgccca 
      cgaatgcgg gtggtgata gttatgttg ggtttaatg 
      tggatgatg aaatcatgg cggcgatta ggaccgagc 
      ttttgaata agttgccgc aggctcagg gtggttcca 
      atggggttg ttcaggatg aggaggagt gggattcgg 
      tggagagga atgaggtgt ttttgaggt ataatgttt 
      acaatacgc cgttatcgc caattccgg aggaaagtc 
      tgcttgagg ataattctt atacaccaa ggcaacagg 
      aaagcgatg acgaagaca gcgattggt cggaccaat 
      gcgaacgcg aagttttac cacacgagc tggaaagtc 
      actcagccc aaactctcg aaagtccag ttcatcccg 
      aacgtctcc acagacaca cacccagcc aggattcct 
      gttgttcaa acgacactc tcttcaact tggaagagc 
      tcacatgtg gcgttaatg actcagaaa gggacaata 
      attccccaa cccctcgaa tctccgaat gtgaatcac 
      aaaccggcg aggaacaac aggtagcaa acaacgcga 
      tgattcatg acataaaca atgactcaa acattggag 
      ccttaaatg acaaaacta caacaacgg agaaagata 
      gtgataatt cggcaagtg ggtgcagaa caagtgaga 
      cagtgaaag aaagtgttg agatgaaac aaacgattg 
      agtttctgg cacgtgaaa gagaacacg attgttcca 
      aaaccagcg aagaatcca attcggaat cgacaactg 
      atcatcttt taccgtttg ttactaagc aggtggcac 
      cactgccat cgcctgagg acaacccct ggaaacaat 
      taccggcgt ctttgttga gttacgata aggtaatgt 
      tgttaccat cgcctgaag ccgttgaaa aaaacaaca 
      cggaagaaa atctctacg ttaacgttc cgcaaaata 
      attgcgaat cgagcgaat tcgaaaatg tggagaatt 
      tggatgatt attacaacg aggctgatt atgtgaata 
      tagtgaatt tagaaaata gcaaaaact cggctgaat 
      ttatcaaca ataaacaaa ttaacgtcg tgattgaag 
      atgcaagtg agcggagta gtcccgact atgttaata 
      atgatctga ttattaatg atgatgatg ataatgaac 
      atgatattt ttggggttt taaccgata ttaggttta 
      gtggaccaa gtgatagtc aaatgatgt cgacatcat 
      ttagcgaaa gtaatgatt taaatgatg agcaagatt 
      gggatagtg gttcggata cgaggtcta gttcaagtg 
      gagagttta tctggaagg agggtgagc acgaagagt 
      ctctcaagg ccttacgcg ctgccccac aagatgtgt 
      tggaagata ttcacccca tggaaggca tacaagaag 
      aaacttcaa gtgagagta gcgggagtg atgatgatg 
      gtgaaagtc ccgaattcg tcccatcag cttgggaca 
      agtatgcaa tacctgcca aatctgcac tgagatctc 
      cagagaaaa cactcgaaa agactgata aaaaaaaag 
      caaaaggtg tgtggttta aaaaacaaa aatatcatt 
      gtgtttatg aatatccga gagaacccg aaagtccag 
      tactccaca gttttgatt tgtggaagc cacagcccg 
      attatagtt cctttgacg actgggaac tagacggca 
      ctgaccctt gcctccctg ccgataacc aagacgatt 
      gcgatttta cccaaaatt actttcaag ccaaaccta 
      atcgaaacc taaatttgt accaactga gtagcataa 
      cagacttta attcaagcg acccaggcc tggacgacg 
      aatttttca taagtagct cagccaaac ccttcgaca 
      tgatcagtg gcttaagtt cccagttct tccccggaa 
      attggaccg atttgagca aaaccaccg aaaatgcga 
      ccccggaca gtggcgtgg aagatatca caccggccg 
      gttttacag agggcgaat ttaccgccc agtttagaa 
      ccgtaaaac cactgaagc aactagctt ccgaagcag 
      tgaaacgaa gcaaaaagg ttgaagaaa caagaaatc 
      atgacatgt tgggcggtt tgagacaca cgagaaatc 
      ggctaaagc ttgatttac cgccgagtc caagcgcgt 
      tcacttcga gtaaaatgt ttagtattg aaccggtca 
      ttgacccgg ttattaacc gggaaaaac cgacgttta 
      caacgttcg ggaaaagtc ggtttttgg tacaacatg 
      tcgatacgc caccggatg acgaagtga gcaaaagta 
      aaaacgttt cgtttgaag cgttacctt ataaaccaa 
      tccagaaac atataatta aggaagatt tcgaaacga 
      gtcgagaga aaggtaagg tggaaagtg tgcgtggcg 
      aagccagcc tactagata gtgccgatg aagacagtg 
      ggattgagt caagcactc tggaacgaa aactgacta 
      accctgttt aggttcgtc ctttttatt aaacttgtt 
      caggtcctt tgatgcctt aaagtgaaa aaggcagtt 
      cgatgataa ctatttaaa agaggaaat tagattagc 
      gatagagaa atcagcttt aaaccacag ttatttacc 
      ttgcgttgg ttactttat gtgatattg ttttaattg 
      gtctctgtt actgtttta ctctcgaat caaaatggt 
      aacgttaac gtctagact atgcgaata aatgtgatc 
      gattgattg aaacttgcc tgagtttgt tccactagt 
      ctagatgtg agacgggaa ataacttga tgtacaaac 
      ttaaaattt ggcgtccaa ggtgttgtt gtagtttgg 
      tatactcag tatttgtat agtctttcg ttattctca 
      tgtgttact gaatcaatc aaattaaca cacttcatg 
      ttaactcat aaattgtgc cccttaata tattgtggt 
      taataagaa ttatttttt attttgtag ataattgtt 
      gtaggatat gtccgtatc ggacctaat aatgtgatc 
      ggctattca tatttcttg tacttgtag taattgtat 
      tgtaatata ctgtagtaa gttatatta ataaacatt 
      ttattcaga a