Status
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Gene information
General information
TC002136 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 19949710..19957874
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002136
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03312

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002136 RA
      MYGYLAVFC ALLAHCSAS RVLELSDKF SEIYKEGGF 
      WLVKFYAPW CGHCKRLEP VWAQVAQAL YKTNIRVGR 
      VDCTRFPTL ATEFSVNGF PTIKFIKPG EDFTFHGDR 
      SKDDIVNFA IRMAGPPVQ DVTRSESLT NLKQMNQLF 
      FMYVGDQEG LLWEAYYDV ASKLQPHGF FYAASKEIA 
      KQHVDVDEL PAVFVYKES LHYFYPIES GPVDLNESE 
      EAQHLNSSM YKWINEERF ETFPKITRG NINEILQTK 
      KYIVLVVVE ENKLQQISQ EMLEFRDMV ESIIRKKRD 
      KYHKHFQFG WVGNPELAN SIAMQVLPL PHLLVLNST 
      TNHHHIPED DPSQLTADA IDIFLERIY NQSVPAYGG 
      NSITVRLYR TYFEARTSL ADMWRGNPV LTTVLFGLP 
      LGFLSLILY SICCSDILD ADEDEEEEL LHEKKE
    CDS sequence
    • >TC002136 RA
      atgtacggt tatttggca gttttttgt gctctgttg 
      gcccactgt tccgcttcc agagtccta gaattaagc 
      gacaaattc tccgaaatt tacaaagag ggtggcttc 
      tggttggtc aaattctac gccccgtgg tgtggtcat 
      tgcaaaaga ttggagccg gtttgggca caggttgcc 
      caagcgttg tataaaacc aacataaga gttggtaga 
      gtggactgt acgaggttt ccaacatta gccaccgaa 
      ttttccgta aatggcttc cccactatc aagtttatt 
      aagccaggt gaagatttt acatttcat ggggacaga 
      agcaaagac gatattgtt aattttgcc atcagaatg 
      gctgggcca cctgtgcaa gatgtgacg agatcagag 
      agtttaaca aacttgaag caaatgaat cagttgttt 
      ttcatgtat gtgggggac caggagggg ctgctgtgg 
      gaggcctac tacgatgtt gctagtaaa ttgcaaccg 
      catgggttt ttctacgcc gctagcaag gaaattgcg 
      aaacaacat gttgatgtt gatgaattg ccggctgtt 
      tttgtgtat aaggaaagt ttgcattac ttctatcca 
      attgaatca ggcccagtt gatttgaac gaatccgag 
      gaagcacaa cacttgaat tcttccatg tataaatgg 
      ataaacgaa gagcggttt gaaacgttc ccaaaaatc 
      acaagaggc aacataaac gagattctc caaacgaaa 
      aagtacatt gtcctggtt gttgttgaa gaaaataaa 
      ttgcaacaa ataagtcaa gagatgttg gaatttaga 
      gatatggtc gagtccatc atacggaag aaaagggat 
      aaataccat aaacatttt caatttggt tgggttggt 
      aaccctgaa ttggcaaac agcatagca atgcaggtc 
      ttaccattg ccgcatttg ttggtttta aattcaacc 
      acaaaccac catcatatc cctgaagat gatccatct 
      cagttgact gcagatgct attgatatt ttccttgaa 
      agaatatac aatcaaagt gtaccagct tatggcgga 
      aatagtata accgttcgc ctctataga acatacttt 
      gaggcaaga acatcactg gctgacatg tggcgggga 
      aatcctgtc ttgacaaca gtccttttt ggtctgcct 
      cttggcttt ctctccttg atattgtac tcaatttgt 
      tgttccgat attttggac gcggatgaa gatgaggaa 
      gaagaatta ttacacgag aaaaaagag taa
    mRNA sequence
    • >TC002136 RA
      gataaatta taataagaa ttatctgtg gtctgacgg 
      agacggaca tgttgttgt gttgtagcg agatttgtt 
      tgttatatt acaggtgtt gtaattttg acggtttct 
      ggcgtacag ctccagttt aaagtggtt gaaagttag 
      cacacggcg accgtgcag ccaaagacc gcaggagaa 
      gtaacaggc aggtgacgc cttagcaca gtgaaccca 
      aaatggcgg tcttgcgaa ttacttaca taacgaaac 
      aacacaaat gtcctgtct gctggaagg cgctcggaa 
      gtccaggcg tgttgggcg accaacagg ggtgatgtg 
      taagccaga ttataagac aggtggtct aaatctgct 
      acacgtggc ggccgatac agatgaggg gacaggcgg 
      tctctgagt ctggtaggc gacaagtag taagtgtta 
      ctagtcaca tagggctag taaacctgc tacttttgg 
      cgggcgaga agtatccaa gatgatacg aactcgtgg 
      cgggcagta agtagtaaa gttgtgaca agctgtctt 
      caactttgc tacttctgg cggaggata agataaggg 
      gacatgcgg tctctaaat cttctactc atgaagggc 
      gataagtaa caaaggtgt tagccaggt aatggaagg 
      gacaggctg ctactgctg ttccttctg gagggtgat 
      aaggcgtaa cgccagaca aggggtcag gcggtcttt 
      aagtctgac tcgtagcgg gcgataagt agcaaaggc 
      gtaacgagc cagataagg gggcaggcg gtctctaag 
      tctggttct catggcggg cgttaacct gaaaagaac 
      caatacaag tggtttcca aatctatga cttactgtg 
      ggcgaagaa gaacgtacc cattgaaga caatctaac 
      ttgaaatgt ccataaaat atgaccaag accataata 
      agggaccaa aacatgatg ttttgtgat attcccacc 
      actggtgac cagtcattc cctgaagag gaatggggg 
      tcatggact gacgaatag gaacttccg gttttggtg 
      aagatgggc acaagtatt ttggcatga tgtgtaata 
      ctatttcca caatacaat aaagttttg gaaacccaa 
      ataagtttt tcaatttgt tttggtcct ttccctact 
      cgaattatc aactttcta gtttactgg aatgtggtg 
      attttggtc gcacatgcg cattaaagt acatttggc 
      accgaatgt ttattagac ttcttgtta ttctgtgac 
      ttcttgtta tttgttttg ggattctgg ggcgtaatc 
      ctagttatt gtgcttgca catcctgtt gtaataagt 
      aataaaggg tgtgattta tcctagtaa tagaaaaaa 
      gaagaatta gtttttatc ggtgtttat actgtggtg 
      gttattttc tcatacccg gctattacc ccttgtgag 
      tttgttata aagggggcc cagttttac acccaaaat 
      aatttataa aataaatta atgcaaagt tactaatgt 
      ggggcaact gggtcacgt ttaaatcag attatggga 
      caaatcaat aaattcgtg attagatta agttaaaac 
      gcaaaactt tgtaatgca cacatgcac aagaatcga 
      ctaataaaa tcgtcaaac atgtacggt tatttggca 
      gttttttgt gctctgttg gcccactgt tccgcttcc 
      agagtccta gaattaagc gacaaattc tccgaaatt 
      tacaaagag ggtggcttc tggttggtc aaattctac 
      gccccgtgg tgtggtcat tgcaaaaga ttggagccg 
      gtttgggca caggttgcc caagcgttg tataaaacc 
      aacataaga gttggtaga gtggactgt acgaggttt 
      ccaacatta gccaccgaa ttttccgta aatggcttc 
      cccactatc aagtttatt aagccaggt gaagatttt 
      acatttcat ggggacaga agcaaagac gatattgtt 
      aattttgcc atcagaatg gctgggcca cctgtgcaa 
      gatgtgacg agatcagag agtttaaca aacttgaag 
      caaatgaat cagttgttt ttcatgtat gtgggggac 
      caggagggg ctgctgtgg gaggcctac tacgatgtt 
      gctagtaaa ttgcaaccg catgggttt ttctacgcc 
      gctagcaag gaaattgcg aaacaacat gttgatgtt 
      gatgaattg ccggctgtt tttgtgtat aaggaaagt 
      ttgcattac ttctatcca attgaatca ggcccagtt 
      gatttgaac gaatccgag gaagcacaa cacttgaat 
      tcttccatg tataaatgg ataaacgaa gagcggttt 
      gaaacgttc ccaaaaatc acaagaggc aacataaac 
      gagattctc caaacgaaa aagtacatt gtcctggtt 
      gttgttgaa gaaaataaa ttgcaacaa ataagtcaa 
      gagatgttg gaatttaga gatatggtc gagtccatc 
      atacggaag aaaagggat aaataccat aaacatttt 
      caatttggt tgggttggt aaccctgaa ttggcaaac 
      agcatagca atgcaggtc ttaccattg ccgcatttg 
      ttggtttta aattcaacc acaaaccac catcatatc 
      cctgaagat gatccatct cagttgact gcagatgct 
      attgatatt ttccttgaa agaatatac aatcaaagt 
      gtaccagct tatggcgga aatagtata accgttcgc 
      ctctataga acatacttt gaggcaaga acatcactg 
      gctgacatg tggcgggga aatcctgtc ttgacaaca 
      gtccttttt ggtctgcct cttggcttt ctctccttg 
      atattgtac tcaatttgt tgttccgat attttggac 
      gcggatgaa gatgaggaa gaagaatta ttacacgag 
      aaaaaagag taatttaaa actaaagta cctaaaaca 
      ttctagtcc tgctggtgt gttaagtgt gtagacaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY