Status
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Gene information
General information
TC002135 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 19944087..19950824
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002135
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03311

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002135 RA
      MASNFDDCS SINSSSAQL SVRNQTKEK FESLLKAAV 
      RKKRPTEVN QSREQLLES PSGSAENLS NDKKSGKKL 
      RKESENYIL NKFLDDSSD HCHTYEIAS EGSQNSSQI 
      KAPIPKPRT KKPASTTSQ RTFEVTSPK IVKNDNGSQ 
      VSNATFNVE EIGDVLVHK SPLKAKIIV KENVKARLS 
      DKSESNDES SESEINENT KKKKLIKIE SEEQKVKKS 
      YTYDKIVEV TILKTDRLQ LSSLVVHPI IKLHVIDAM 
      TGKYFPKSD KSRSVVFFY ENSDNDYIS PVMTRAYNL 
      QEKRILYPL WEESILLNE DFEYFKDES NRIILFFEL 
      LDFVSVSTL HQLNQKECV KGWHNIAFA FLKLAGKNG 
      ELNIGKNLR LQLYHSHQT KKNDPQVCN AWVWWKKKK 
      LKKYPSTLH IAIKSVVSP TKVVETFRS KTPIQRETS 
      SRTKLCDEI QNNQILNPE GAIVSVENK SDEKNKETL 
      LTWSRKRNE VCKFPNKCL VKFNSLEQG CFVMKFSPS 
      GKYLACAVQ NEDLFYVIV YSIVSLNEV NRFPSHQAI 
      IYCLRWFSD DVLLLSASA DNTVCIHNL SDSSFQILP 
      HPSFVYCCD ISKDKVIVT GCYDGIIRI WNQTPSDKK 
      SEFHLYQEL EAHKAYITS LCWNKKSSI FSADSTGAI 
      IEWQKKDND WVLKREINL LDLKETVIN QILLFPNEK 
      RLLVHSRDN IIRIIDLKS GCVLHWLRG ASNKRFQLF 
      CSISPCGTY VNSGSENGF VHVWNAKNG HEVAVYLPY 
      ASDHQFLTI HCVDFHPYD NMVAISHYG RNLPVLIFC 
      FDKNIEKPE VELTFKENV EVEYVKNQQ SSKELRLNG 
      SIKRERCSN KNEEKIDFK AILHKMDVV LTLQKN
    CDS sequence
    • >TC002135 RA
      atggcttcg aattttgat gattgttct tcaattaat 
      agttcaagt gctcaatta agtgtaagg aatcagaca 
      aaggaaaaa tttgaatcg ttgcttaaa gccgccgtg 
      cgtaaaaaa cgcccaact gaagtgaac cagtccagg 
      gaacaactt ttggagtct ccctctggt agtgctgaa 
      aatttatca aatgataaa aaatcaggc aagaaatta 
      cgtaaagaa agtgaaaat tatattctg aacaaattt 
      cttgatgac tcctctgac cactgccac acgtatgaa 
      attgcctct gagggctcg cagaactcg agtcagatt 
      aaagcccca atcccaaaa ccaagaacg aaaaaacct 
      gcatcgacc accagtcaa cgaacgttc gaagtgacg 
      tctccaaaa attgtgaaa aatgataat gggagtcaa 
      gtgagtaac gccacgttc aatgttgag gaaataggg 
      gatgttttg gtgcataaa agtcccctt aaagcgaaa 
      ataattgtg aaagaaaat gttaaggca agactttcg 
      gacaaaagt gagagtaat gatgaatct tcagaatca 
      gaaattaat gagaacact aaaaagaag aaactaata 
      aaaattgaa tctgaagaa cagaaagtg aagaaaagt 
      tacacgtat gataaaata gtggaggtc acaattctg 
      aaaactgac cggcttcag ctcagttct ttggtggtt 
      catccgata ataaaactt cacgttatt gatgcaatg 
      acggggaaa tattttccg aaaagtgat aaaagtcgc 
      tccgttgta tttttttac gaaaacagt gataatgat 
      tatatttcg ccagtgatg acacgtgct tataattta 
      caggaaaaa aggatctta tatccttta tgggaagaa 
      agcatttta ttaaatgag gattttgag tacttcaaa 
      gatgaaagt aatcggata atattattt tttgagctc 
      ttggacttc gtttctgtt tcaacactg caccaacta 
      aaccaaaaa gaatgtgta aaaggttgg cacaatatc 
      gcgtttgcc ttcctcaag ctagctgga aaaaacggc 
      gaactaaat attggcaaa aatttaagg ctccagttg 
      taccattct caccaaaca aagaaaaac gaccctcaa 
      gtgtgtaat gcctgggtt tggtggaag aaaaaaaaa 
      ttgaaaaaa tatccatca actttacac attgccatt 
      aaatcggtg gtatcaccg actaaagtt gtggaaaca 
      tttcgttcg aaaacaccg attcagaga gagactagt 
      tcacgaaca aaactgtgt gacgaaatt caaaataat 
      caaatttta aatcctgag ggagcaatc gtgagcgtg 
      gaaaataag tcagatgaa aaaaataag gaaacactg 
      ctgacttgg tctaggaaa cgtaacgaa gtttgcaag 
      tttccgaat aagtgtttg gtaaaattt aacagtctt 
      gagcaaggc tgttttgtt atgaaattt agtcctagt 
      ggaaaatat ctagcgtgc gctgtccag aatgaagat 
      ttattttac gttattgtt tattcgata gtgtcacta 
      aacgaagtc aataggttt ccatcacac caggcaata 
      atttactgc ctcagatgg tttagtgac gatgtcctg 
      cttttatca gcgagtgct gataataca gtttgtatt 
      cacaacttg tctgactct tcttttcaa attttacca 
      catccatct tttgtatac tgctgtgat ataagtaaa 
      gacaaagtg atagttact ggttgctac gatgggata 
      ataagaatt tggaaccaa acaccttct gataaaaaa 
      tcagaattc catttgtat caagaattg gaagctcat 
      aaagcatac attacgtcc ctctgctgg aacaaaaaa 
      tctagcatt ttttcggca gattcgaca ggtgctatt 
      atagaatgg caaaagaaa gacaacgat tgggtgtta 
      aaaagagaa ataaatttg ctggattta aaagaaaca 
      gttataaac cagatactg ctatttccg aacgaaaaa 
      agactactg gtgcattct cgtgataac ataattcga 
      ataattgat ttaaaaagt gggtgtgtt ttacattgg 
      ctgcgtgga gcgtccaac aaaagattt cagttattc 
      tgttccatt tcgccttgc ggaacttat gttaattcc 
      ggtagcgaa aatggattt gttcacgtg tggaatgca 
      aaaaatggg catgaggtt gctgtgtac cttccttat 
      gcgtcagat catcagttt ttgaccata cattgtgtc 
      gattttcac ccatatgat aacatggta gcaatttct 
      cactatggg aggaatcta ccagtttta atcttctgt 
      tttgataag aatatagaa aagcctgaa gttgagctc 
      acttttaaa gaaaacgtc gaagttgaa tacgtaaag 
      aatcaacag tctagtaaa gaattgaga ttaaatgga 
      agtataaaa agggaacga tgttctaac aaaaatgag 
      gaaaagata gactttaag gccatttta cacaaaatg 
      gatgtggtg ctaacatta cagaaaaat taa
    mRNA sequence
    • >TC002135 RA
      caatacaac aaatagaag tttttgtga caatttcat 
      ttgcttgat ggcttcgaa ttttgatga ttgttcttc 
      aattaatag ttcaagtgc tcaattaag tgtaaggaa 
      tcagacaaa ggaaaaatt tgaatcgtt gcttaaagc 
      cgccgtgcg taaaaaacg cccaactga agtgaacca 
      gtccaggga acaactttt ggagtctcc ctctggtag 
      tgctgaaaa tttatcaaa tgataaaaa atcaggcaa 
      gaaattacg taaagaaag tgaaaatta tattctgaa 
      caaatttct tgatgactc ctctgacca ctgccacac 
      gtatgaaat tgcctctga gggctcgca gaactcgag 
      tcagattaa agccccaat cccaaaacc aagaacgaa 
      aaaacctgc atcgaccac cagtcaacg aacgttcga 
      agtgacgtc tccaaaaat tgtgaaaaa tgataatgg 
      gagtcaagt gagtaacgc cacgttcaa tgttgagga 
      aatagggga tgttttggt gcataaaag tccccttaa 
      agcgaaaat aattgtgaa agaaaatgt taaggcaag 
      actttcgga caaaagtga gagtaatga tgaatcttc 
      agaatcaga aattaatga gaacactaa aaagaagaa 
      actaataaa aattgaatc tgaagaaca gaaagtgaa 
      gaaaagtta cacgtatga taaaatagt ggaggtcac 
      aattctgaa aactgaccg gcttcagct cagttcttt 
      ggtggttca tccgataat aaaacttca cgttattga 
      tgcaatgac ggggaaata ttttccgaa aagtgataa 
      aagtcgctc cgttgtatt tttttacga aaacagtga 
      taatgatta tatttcgcc agtgatgac acgtgctta 
      taatttaca ggaaaaaag gatcttata tcctttatg 
      ggaagaaag cattttatt aaatgagga ttttgagta 
      cttcaaaga tgaaagtaa tcggataat attattttt 
      tgagctctt ggacttcgt ttctgtttc aacactgca 
      ccaactaaa ccaaaaaga atgtgtaaa aggttggca 
      caatatcgc gtttgcctt cctcaagct agctggaaa 
      aaacggcga actaaatat tggcaaaaa tttaaggct 
      ccagttgta ccattctca ccaaacaaa gaaaaacga 
      ccctcaagt gtgtaatgc ctgggtttg gtggaagaa 
      aaaaaaatt gaaaaaata tccatcaac tttacacat 
      tgccattaa atcggtggt atcaccgac taaagttgt 
      ggaaacatt tcgttcgaa aacaccgat tcagagaga 
      gactagttc acgaacaaa actgtgtga cgaaattca 
      aaataatca aattttaaa tcctgaggg agcaatcgt 
      gagcgtgga aaataagtc agatgaaaa aaataagga 
      aacactgct gacttggtc taggaaacg taacgaagt 
      ttgcaagtt tccgaataa gtgtttggt aaaatttaa 
      cagtcttga gcaaggctg ttttgttat gaaatttag 
      tcctagtgg aaaatatct agcgtgcgc tgtccagaa 
      tgaagattt attttacgt tattgttta ttcgatagt 
      gtcactaaa cgaagtcaa taggtttcc atcacacca 
      ggcaataat ttactgcct cagatggtt tagtgacga 
      tgtcctgct tttatcagc gagtgctga taatacagt 
      ttgtattca caacttgtc tgactcttc ttttcaaat 
      tttaccaca tccatcttt tgtatactg ctgtgatat 
      aagtaaaga caaagtgat agttactgg ttgctacga 
      tgggataat aagaatttg gaaccaaac accttctga 
      taaaaaatc agaattcca tttgtatca agaattgga 
      agctcataa agcatacat tacgtccct ctgctggaa 
      caaaaaatc tagcatttt ttcggcaga ttcgacagg 
      tgctattat agaatggca aaagaaaga caacgattg 
      ggtgttaaa aagagaaat aaatttgct ggatttaaa 
      agaaacagt tataaacca gatactgct atttccgaa 
      cgaaaaaag actactggt gcattctcg tgataacat 
      aattcgaat aattgattt aaaaagtgg gtgtgtttt 
      acattggct gcgtggagc gtccaacaa aagatttca 
      gttattctg ttccatttc gccttgcgg aacttatgt 
      taattccgg tagcgaaaa tggatttgt tcacgtgtg 
      gaatgcaaa aaatgggca tgaggttgc tgtgtacct 
      tccttatgc gtcagatca tcagttttt gaccataca 
      ttgtgtcga ttttcaccc atatgataa catggtagc 
      aatttctca ctatgggag gaatctacc agttttaat 
      cttctgttt tgataagaa tatagaaaa gcctgaagt 
      tgagctcac ttttaaaga aaacgtcga agttgaata 
      cgtaaagaa tcaacagtc tagtaaaga attgagatt 
      aaatggaag tataaaaag ggaacgatg ttctaacaa 
      aaatgagga aaagataga ctttaaggc cattttaca 
      caaaatgga tgtggtgct aacattaca gaaaaatta 
      agttaccta cttaagtta tacctactt ttatactgc 
      tgtagtcga taaattata ataagaatt atctgtggt 
      ctgacggag acggacatg ttgttgtgt tgtagcgag 
      atttgtttg ttatattac aggtgttgt aattttgac 
      ggtttctgg cgtacagct ccagtttaa agtggttga 
      aagttagca cacggcgac cgtgcagcc aaagaccgc 
      aggagaagt aacaggcag gtgacgcct tagcacagt 
      gaacccaaa atggcggtc ttgcgaatt acttacata 
      acgaaacaa cacaaatgt cctgtctgc tggaaggcg 
      ctcggaagt ccaggcgtg ttgggcgac caacagggg 
      tgatgtgta agccagatt ataagacag gtggtctaa 
      atctgctac acgtggcgg ccgatacag atgagggga 
      caggcggtc tctgagtct ggtaggcga caagtagta 
      agtgttact agtcacata gggctagta aacctgcta 
      cttttggcg ggcgagaag tatccaaga tgatacgaa 
      ctcgtggcg ggcagtaag tagtaaagt tgtgacaag 
      ctgtcttca actttgcta cttctggcg gaggataag 
      ataagggga catgcggtc tctaaatct tctactcat 
      gaagggcga taagtaaca aaggtgtta gccaggtaa 
      tggaaggga caggctgct actgctgtt ccttctgga 
      gggtgataa ggcgtaacg ccagacaag gggtcaggc 
      ggtctttaa gtctgactc gtagcgggc gataagtag 
      caaaggcgt aacgagcca gataagggg gcaggcggt 
      ctctaagtc tggttctca tggcgggcg ttaacctga 
      aaagaacca atacaagtg gtttccaaa tctatgact 
      tactgtggg cgaagaaga acgtaccca ttgaagaca 
      atctaactt gaaatgtcc ataaaatat gaccaagac 
      cataataag ggaccaaaa catgatgtt ttgtgatat 
      tcccaccac tggtgacca gtcattccc tgaagagga 
      atgggggtc atggactga cgaatagga acttccggt 
      tttggtgaa gatgggcac aagtatttt ggcatgatg 
      tgtaatact atttccaca atacaataa agttttgga 
      aaccca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY