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Gene information
General information
TC032605
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC002119
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 20052326..20061215
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032605
    iBeetle screen iB_03309

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    larva partially dorsalnot closed - Penetrance (percentage of animals/eggs on slide): less than 30%

    head capsule size decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head appendages random not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032605 RA
      MFTEPVFDN KWQKCKFCS STFEELSDY LTHIELVHK 
      NHKTNYLLS SFMCSDCGK QYKTKNELT NHINSKCGT 
      VKQFKCKIC GQELMSAGS LYNHMLRHN GVRSFMCRF 
      CAKLFFTAG QLKVHERIH TQDKAYVCE VCNKGFCHR 
      QSLITHSTI HTGIKPYQC ENCGNSFSC VGNLIKHRK 
      THADTCGLI PLTTHRVKH PSTKIKVKI NTPSNSRLK 
      TNGQMDFEV QLEVEKELN ILKSALCRN NKTEGKKEA 
      TNDEQCAKQ NLIDKIQEC IQRSDTEES LSENNSNEN 
      VEKNTALND TPKEVAQEI TLSTGGGVK STKRRHVER 
      DRNYENEIS KFTEQTNFD DEGFGDCKY CGKRYMNVR 
      WLYKHVKEH ENNIPIGSD QSGIPLHKC SCCKMSFYT 
      KEESLIHQQ THHADILTC HECEKIFSN RDSLRSHQK 
      VFHKGVGRK AYIYVCDKC GKQFKQKSY LKAHEERNC 
      DKGPFYECS ICQKQFSSV YTRNNHMRV HDPEKKLLC 
      KFCAKSFHW KGQLKIHER SHTGEKPFA CLYCPKAFA 
      YRESLITHS TIHTGIKPH LCEGCGARF SCIGNLIKH 
      RSSHANECG AWAYKTQKP LQSN
    CDS sequence
    • >TC032605 RA
      atgttcacc gagccagtg tttgacaac aaatggcaa 
      aaatgtaaa ttttgctcc tcaactttt gaagaattg 
      agcgactat ttaacgcac attgaattg gtccataaa 
      aaccacaaa accaattat ttattgtcg agttttatg 
      tgtagcgat tgtggtaaa caatacaaa accaaaaac 
      gagctcaca aaccacatc aattccaaa tgcgggact 
      gtgaaacaa ttcaaatgc aaaatttgt ggccaggaa 
      ctgatgtct gccggttcg ctctacaat cacatgctg 
      agacataac ggagtccga agtttcatg tgcagattt 
      tgcgccaaa ttgtttttt acagcaggg cagttgaaa 
      gtgcatgag cggatccat acccaggac aaggcctac 
      gtttgtgag gtttgtaat aaggggttt tgtcaccgg 
      caaagtctc atcactcac agtaccatc catacaggg 
      attaaaccg taccagtgt gagaattgt gggaattcg 
      tttagttgt gtggggaat ttgattaaa cacaggaag 
      actcatgcg gatacgtgt ggtcttatt ccgttgact 
      acacacagg gttaagcat ccttccact aaaattaaa 
      gttaagata aatacgccg tctaattcg aggctgaag 
      acgaatgga caaatggac tttgaagtg caactggaa 
      gtggagaag gagttgaat attttgaag agtgcttta 
      tgtagaaat aataagact gaagggaag aaggaagct 
      acaaatgat gaacagtgt gccaaacaa aatctcatt 
      gacaaaata caagaatgc atccaaagg agcgacact 
      gaagagtca ttaagtgaa aacaactcc aatgaaaat 
      gttgaaaaa aatacagca cttaatgac acaccaaaa 
      gaagttgca caagagatt acgttatcc acaggtgga 
      ggagtgaaa agtaccaaa agaagacat gtggaaagg 
      gatcgaaac tatgaaaat gagatatcg aaatttaca 
      gaacagaca aactttgat gatgaagga ttcggtgat 
      tgtaaatac tgtggtaag agatatatg aacgtccgt 
      tggctttac aaacacgtg aaagaacat gaaaataac 
      attccaatc ggatccgac caatcagga atccctctc 
      cacaaatgt agctgttgt aaaatgagt ttttacaca 
      aaggaagaa agtctcatc caccaacaa acacaccat 
      gccgatatt ttaacatgt cacgaatgc gagaaaatt 
      ttctccaac cgcgactcc ttacgctcg caccagaaa 
      gtcttccat aaaggagtc ggtcgtaaa gcttacatc 
      tacgtatgc gacaagtgc ggaaaacag ttcaaacaa 
      aaatcctac ctgaaagcc cacgaagag cgaaactgt 
      gataaaggc cccttttac gaatgctcc atctgccag 
      aaacagttc tccagtgtt tacaccagg aacaaccac 
      atgcgggtg cacgatccg gagaagaag ctcctgtgc 
      aagttctgt gccaaaagt ttccactgg aagggacag 
      ctgaagatc catgaacgt agtcacacc ggagagaag 
      ccttttgct tgcttgtat tgtcccaaa gctttcgcg 
      tatagggaa agtctgatt acgcacagt accatccat 
      acggggata aagcctcat ctgtgtgaa gggtgtggt 
      gctaggttt tcctgtata gggaattta attaaacat 
      cggagtagt catgcgaat gaatgtggg gcttgggct 
      tacaaaaca cagaaacct ttacaaagt aattaa
    mRNA sequence
    • >TC032605 RA
      cggcgccaa aatccccaa caaaatggc tgctccgtt 
      atttagcag tgtatgagt gtaaacaat tacgtttcg 
      aacgagtgt aaatgaaat tcaaatatt tttttgtaa 
      aaacaataa cttaaccaa ctggtaatt ggtgaataa 
      atcatacaa taaattcgt aaattaaaa aaatgttca 
      ccgagccag tgtttgaca acaaatggc aaaaatgta 
      aattttgct cctcaactt ttgaagaat tgagcgact 
      atttaacgc acattgaat tggtccata aaaaccaca 
      aaaccaatt atttattgt cgagtttta tgtgtagcg 
      attgtggta aacaataca aaaccaaaa acgagctca 
      caaaccaca tcaattcca aatgcggga ctgtgaaac 
      aattcaaat gcaaaattt gtggccagg aactgatgt 
      ctgccggtt cgctctaca atcacatgc tgagacata 
      acggagtcc gaagtttca tgtgcagat tttgcgcca 
      aattgtttt ttacagcag ggcagttga aagtgcatg 
      agcggatcc atacccagg acaaggcct acgtttgtg 
      aggtttgta ataaggggt tttgtcacc ggcaaagtc 
      tcatcactc acagtacca tccatacag ggattaaac 
      cgtaccagt gtgagaatt gtgggaatt cgtttagtt 
      gtgtgggga atttgatta aacacagga agactcatg 
      cggatacgt gtggtctta ttccgttga ctacacaca 
      gggttaagc atccttcca ctaaaatta aagttaaga 
      taaatacgc cgtctaatt cgaggctga agacgaatg 
      gacaaatgg actttgaag tgcaactgg aagtggaga 
      aggagttga atattttga agagtgctt tatgtagaa 
      ataataaga ctgaaggga agaaggaag ctacaaatg 
      atgaacagt gtgccaaac aaaatctca ttgacaaaa 
      tacaagaat gcatccaaa ggagcgaca ctgaagagt 
      cattaagtg aaaacaact ccaatgaaa atgttgaaa 
      aaaatacag cacttaatg acacaccaa aagaagttg 
      cacaagaga ttacgttat ccacaggtg gaggagtga 
      aaagtacca aaagaagac atgtggaaa gggatcgaa 
      actatgaaa atgagatat cgaaattta cagaacaga 
      caaactttg atgatgaag gattcggtg attgtaaat 
      actgtggta agagatata tgaacgtcc gttggcttt 
      acaaacacg tgaaagaac atgaaaata acattccaa 
      tcggatccg accaatcag gaatccctc tccacaaat 
      gtagctgtt gtaaaatga gtttttaca caaaggaag 
      aaagtctca tccaccaac aaacacacc atgccgata 
      ttttaacat gtcacgaat gcgagaaaa ttttctcca 
      accgcgact ccttacgct cgcaccaga aagtcttcc 
      ataaaggag tcggtcgta aagcttaca tctacgtat 
      gcgacaagt gcggaaaac agttcaaac aaaaatcct 
      acctgaaag cccacgaag agcgaaact gtgataaag 
      gcccctttt acgaatgct ccatctgcc agaaacagt 
      tctccagtg tttacacca ggaacaacc acatgcggg 
      tgcacgatc cggagaaga agctcctgt gcaagttct 
      gtgccaaaa gtttccact ggaagggac agctgaaga 
      tccatgaac gtagtcaca ccggagaga agccttttg 
      cttgcttgt attgtccca aagctttcg cgtataggg 
      aaagtctga ttacgcaca gtaccatcc atacgggga 
      taaagcctc atctgtgtg aagggtgtg gtgctaggt 
      tttcctgta tagggaatt taattaaac atcggagta 
      gtcatgcga atgaatgtg gggcttggg cttacaaaa 
      cacagaaac ctttacaaa gtaattaaa ttgtactga 
      agtctttat gtgtttttt ttatcgcac cttaagcaa 
      tattctaca cgatttctt acaaaaaaa tgggaaaat 
      gttgccacc aagcaggaa aaagaggta aaaaaagaa 
      aattgaatc aaacctcta ccacatggg cgagaaaag 
      caatgaatt aataaataa gagttttat tc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY