Status
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Gene information
General information
TC002099 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015452015.1: 4..2870
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002099
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03304

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    -
    larva partially dorsalnot closed - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larva partially constricted - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    thorax segment partially randomnot present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002099 RA
      MQLTVSSGG LKATTKDHG LTEYWAHRL TTCSAPAQF 
      PRLFCWVYR HEGRRLRHE LRCHAVLCP SAAIARLIE 
      TQLKQALAL ALAEFKRDK ISRQNARLS LVNSVYDNP 
      TIPRRKILL STGSHNYKP PLERSKSAP KLTSIEEII 
      EEEEETPEP SKNVYKKIL RHYDSTSAL LDKRKTFVR 
      RLQVPDVRE DDKELTLDE KSDMILEEL VQNSLRNDL 
      LESCERAWF NALGEKPDL VPPDSDEGS LSSGCESAS 
      TVTSDLDQP VFPAISEED EPKKTDLDF KVGGCVLAR 
      VRSFERVNA YCGLKILGN EEVSLVPVG ESQADFSSL 
      KVFKRRSLD TRELCEEEG ENESACSDE SGYEEEEVN 
      SSVANIVLV
    • >TC002099 RB
      MILEELVQN SLRNDLLES CERAWFNAL GEKPDLVPP 
      DSDEGSLSS GCESASTVT SDLDQPVFP AISEEDEPK 
      KTDLDFKVG GCVLARVRS FERVNAYCG LKILGNEEV 
      SLVPVGESQ ADFSSLKVF KRRSLDTRE LCEEEGENE 
      SACSDESGY EEEEVNSSV ANIVLV
    CDS sequence
    • >TC002099 RA
      atgcagctc actgtgagc agcggcggc ctcaaagcc 
      accaccaaa gaccacggc ctcaccgaa tactgggcc 
      caccgtctc accacctgc tccgccccc gcccaattc 
      ccgcgcctc ttctgctgg gtgtaccgc cacgagggc 
      cgccgcctc cggcacgaa ctccgctgc cacgcggtg 
      ctctgcccc tcggccgcc atcgcccgc ctcatcgaa 
      acccagctc aagcaagcc ctcgccctg gccttggcc 
      gagttcaaa cgcgacaaa atctcgcgg caaaacgca 
      cgactctcc cttgttaac tcggtctac gacaacccc 
      accatcccc aggaggaaa atcctgcta agtacgggc 
      tcccacaac tacaagccg cccttggag cggtcgaaa 
      tccgccccg aagttaacc agcatcgag gaaattatc 
      gaggaggag gaagagact cctgaaccg agcaaaaac 
      gtgtacaag aagattctg cggcattat gacagcacg 
      agtgctttg ctcgacaag cgaaaaact ttcgtccgg 
      aggctgcaa gtacctgac gtccgtgag gacgacaaa 
      gagctgact cttgacgag aaaagcgac atgattttg 
      gaggagctg gtgcagaat tcgctccgg aacgacctt 
      ttggagtcg tgtgaaagg gcctggttt aacgctttg 
      ggggagaag cccgacctg gtgccccct gatagtgac 
      gaggggtcg ttgtcaagc gggtgtgag tcggcgtcc 
      acggtcacc tctgacctg gaccagccc gttttcccg 
      gccatctcc gaggaggac gagccgaaa aagactgat 
      ttggacttc aaagttggg ggctgcgtg ctggcccgc 
      gttcgcagc tttgagagg gttaacgcg tattgcggt 
      ttgaagatt ctggggaat gaggaggtg tcgctggtg 
      ccggtgggg gagagccag gccgatttc agttccttg 
      aaggtgttc aagcggagg agtttggac acgagggag 
      ttgtgtgag gaggagggc gagaatgag agtgcgtgt 
      agtgacgag agcgggtac gaggaggag gaggtgaat 
      agttcggtc gctaatatt gtgctagtt tag
    • >TC002099 RB
      atgattttg gaggagctg gtgcagaat tcgctccgg 
      aacgacctt ttggagtcg tgtgaaagg gcctggttt 
      aacgctttg ggggagaag cccgacctg gtgccccct 
      gatagtgac gaggggtcg ttgtcaagc gggtgtgag 
      tcggcgtcc acggtcacc tctgacctg gaccagccc 
      gttttcccg gccatctcc gaggaggac gagccgaaa 
      aagactgat ttggacttc aaagttggg ggctgcgtg 
      ctggcccgc gttcgcagc tttgagagg gttaacgcg 
      tattgcggt ttgaagatt ctggggaat gaggaggtg 
      tcgctggtg ccggtgggg gagagccag gccgatttc 
      agttccttg aaggtgttc aagcggagg agtttggac 
      acgagggag ttgtgtgag gaggagggc gagaatgag 
      agtgcgtgt agtgacgag agcgggtac gaggaggag 
      gaggtgaat agttcggtc gctaatatt gtgctagtt 
      tag
    mRNA sequence
    • >TC002099 RA
      agtgacttt aaaatgcaa attctacaa aagccagca 
      ttttagaat aagttgtat gtattaaat ttgacagaa 
      aaattttca aaatctgtt gtcactgtc aaaaacata 
      cgctggcag gacctgtca atggcaatg gcactccgc 
      aataaaact agtaccgca tcaaatttc acttattcc 
      ctcggattt tcctttaaa tattttttt tcacaaggt 
      ttcaattgt caaaatgta gaataaacc tgtttaaat 
      gtgttgtta ttggaggaa aattgtgaa tattcctta 
      aattaatca taaagaaat tcactaata caaaaaata 
      tttacgaat acacaaaca aaaacgacc ataagcacg 
      ttgaaaacg gctttaaac gaaaataat tgaaggttt 
      tagtggatt aaggaaatg aaaatttgg ttctttttg 
      gcaaagtag aggatggaa cggtattta aaaaaacaa 
      ttattgttg cgtttacaa attcatgac gaaaactgg 
      gtgctttac attagagat agttacaaa aaaaaattg 
      taaaattgg taaaaaaaa attatctct ccgtgcata 
      gaatccact tgttgcttc agttgcaaa cctttatta 
      aaaaaaata cagcacaaa atttcagtg tttccttta 
      aatttttaa attttcaaa tgttcaagt gcttaagtt 
      taaaccgtt aataataat caactaaca gaatatgtg 
      cagttaccg gaaggaaat tgtgaatgt ttcttaaaa 
      tattgtgta atagaaagt agataagag ttttatgca 
      ctaaacgac acataatag gtgattcta agtttttaa 
      tactgattt gaggatatt agagaagtc cattctgtc 
      gctggaaaa tctacgcca caatttatt tcattgttg 
      cgttttcga attcttgac aaaaactta atttaaact 
      tacactccc gcttttttc ccgttgaaa cgtcagcga 
      aaaagtaac aaatagaga ctaatcccc cgttcacag 
      aatccactt gttgctttt attcctcct ctcattctc 
      cgctaatta cctcggcac gttctcact cgttccgtt 
      ttccaggtg acggctgtg tggaaaagc cgttagcga 
      ccttatggc gtaactaca cgcaatcgt cccgtcccg 
      acgtccgca tgcagctca ctgtgagca gcggcggcc 
      tcaaagcca ccaccaaag accacggcc tcaccgaat 
      actgggccc accgtctca ccacctgct ccgcccccg 
      cccaattcc cgcgcctct tctgctggg tgtaccgcc 
      acgagggcc gccgcctcc ggcacgaac tccgctgcc 
      acgcggtgc tctgcccct cggccgcca tcgcccgcc 
      tcatcgaaa cccagctca agcaagccc tcgccctgg 
      ccttggccg agttcaaac gcgacaaaa tctcgcggc 
      aaaacgcac gactctccc ttgttaact cggtctacg 
      acaacccca ccatcccca ggaggaaaa tcctgctaa 
      gtacgggct cccacaact acaagccgc ccttggagc 
      ggtcgaaat ccgccccga agttaacca gcatcgagg 
      aaattatcg aggaggagg aagagactc ctgaaccga 
      gcaaaaacg tgtacaaga agattctgc ggcattatg 
      acagcacga gtgctttgc tcgacaagc gaaaaactt 
      tcgtccgga ggctgcaag tacctgacg tccgtgagg 
      acgacaaag agctgactc ttgacgaga aaagcgaca 
      tgattttgg aggagctgg tgcagaatt cgctccgga 
      acgaccttt tggagtcgt gtgaaaggg cctggttta 
      acgctttgg gggagaagc ccgacctgg tgccccctg 
      atagtgacg aggggtcgt tgtcaagcg ggtgtgagt 
      cggcgtcca cggtcacct ctgacctgg accagcccg 
      ttttcccgg ccatctccg aggaggacg agccgaaaa 
      agactgatt tggacttca aagttgggg gctgcgtgc 
      tggcccgcg ttcgcagct ttgagaggg ttaacgcgt 
      attgcggtt tgaagattc tggggaatg aggaggtgt 
      cgctggtgc cggtggggg agagccagg ccgatttca 
      gttccttga aggtgttca agcggagga gtttggaca 
      cgagggagt tgtgtgagg aggagggcg agaatgaga 
      gtgcgtgta gtgacgaga gcgggtacg aggaggagg 
      aggtgaata gttcggtcg ctaatattg tgctagttt 
      aggggtggg gttggggga gttgtgact attgtagtg 
      tatatattg tatatagtt tcacagaat agaatgtga 
      tgttttgtt gtaatgtgt taccatttg tggtgtaga 
      tcgggtgca catttctta ttgtacctc acttcagac 
      aaagtattt atattgtta tttgacaat gatattgtg 
      tattaaata aatatttga ggaaaat
    • >TC002099 RB
      tcccgcgcc tcttctgct gggtgtacc gccacgagg 
      gccgccgcc tccggcacg aactccgct gccacgcgg 
      tgctctgcc cctcggccg ccatcgccc gcctcatcg 
      aaacccagc tcaagcaag ccctcgccc tggccttgg 
      ccgagttca aacgcgaca aaatctcgc ggcaaaacg 
      cacgactct cccttgtta actcggtct acgacaacc 
      ccaccatcc ccaggagga aaatcctgc taagtacgg 
      gctcccaca actacaagc cgcccttgg agcggtcga 
      aatccgccc cgaagttaa ccagcatcg aggaaatta 
      tcgaggagg aggaagaga ctcctgaac cgagcaaaa 
      acgtgtaca agaagattc tgcggcatt atgacagca 
      cgagtgctt tgctcgaca agcgaaaaa ctttcgtcc 
      ggaggctgc aagtacctg acgtccgtg aggacgaca 
      aagagctga ctcttgacg agaaaagcg acatgattt 
      tggaggagc tggtgcaga attcgctcc ggaacgacc 
      ttttggagt cgtgtgaaa gggcctggt ttaacgctt 
      tgggggaga agcccgacc tggtgcccc ctgatagtg 
      acgaggggt cgttgtcaa gcgggtgtg agtcggcgt 
      ccacggtca cctctgacc tggaccagc ccgttttcc 
      cggccatct ccgaggagg acgagccga aaaagactg 
      atttggact tcaaagttg ggggctgcg tgctggccc 
      gcgttcgca gctttgaga gggttaacg cgtattgcg 
      gtttgaaga ttctgggga atgaggagg tgtcgctgg 
      tgccggtgg gggagagcc aggccgatt tcagttcct 
      tgaaggtgt tcaagcgga ggagtttgg acacgaggg 
      agttgtgtg aggaggagg gcgagaatg agagtgcgt 
      gtagtgacg agagcgggt acgaggagg aggaggtga 
      atagttcgg tcgctaata ttgtgctag tttaggggt 
      ggggttggg ggagttgtg actattgta gtgtatata 
      ttgtatata gtttcacag aatagaatg tgatgtttt 
      gttgtaatg tgttaccat ttgtggtgt agatcgggt 
      gcacatttc ttattgtac ctcacttca gacaaagta 
      tttatattg ttatttgac aatgatatt gtgtattaa 
      ataaatatt tgaggaaaa t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY