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Gene information
General information
TC002085 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid75: 1726296..1737285
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002085
    iBeetle screen iB_03300
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Fertility
    lateral oviduct potentiallystructure irregular - Number of animals showing the phenotype: 4

    egg chamber number decreased - Number of animals showing the phenotype: 4

    egg chamber mostly not present - Number of animals showing the phenotype: 4
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002085 RA
      MWRLLLLAL LAAAEDNKD KDRTVLGSS VVTSVSVIM 
      DAGNGSKIY TDAVGKPVS KPQVATDVA SPIELLNPD 
      RYEFYTFDD AGDLVKRLM TLEEIHDII ATGDDDVTL 
      DAFMPEKRV NDVVNNVQN VLKEEIKQH KNPEKPEFD 
      TPDVSDSWS MILPAIFGN SGADIKPEP PTSHVTPDT 
      VLVMATTEQ ISTTQKVNT LQTSSTSTT TTSTTSSTP 
      LINVEIITN SNSTTERSD NVSPVVEIK PQHESEFKN 
      NVRKGTKRP FPVKSTTFK PLRRPTRTT TLTEAPSTT 
      TTTTTTPAT TTEPVSETI VPDAGAQFE KILPETTTI 
      TTVPTTTTP PTTTELDRK TIVPQFEKI ALETTTTTE 
      PETTSTEMP STTKVVPET TTTTTPTTT QQETSTYGT 
      FSTFFLVSD TKNQSEPDL STLGTEYVT VKTLSTNTT 
      ESTVPDLEQ FLLTSTNIY EINSQLSDS TTEEKTETT 
      EASEKFLIR LTNPTEVPT TTTDQTFIE TIEQLISQA 
      VGTSGPYVE AQKEASMVE EMMKNGGNK TMEEVMAET 
      ATAGSSLLD MVPTKVVPT TMDLSESVD SLLSQVLQV 
      STTLEPETT TVVPTVVPP ESHIINITI IRNEPKPAK 
      ADLSLEMEY DAMLDEMRK NGTFDDSTM PKNGSLVET 
      TTIDSTMPQ NGTLVETTT IDSTTTFDS TTTEETIES 
      STTPSIEIT INEKNESGA TWTLVSTLA PPTSTPPAV 
      DLVPKPLQG FGLEDSTAH LDTDIYQFV QLCNELAFG 
      FWKSVTNGL STARSVVVS PFAATSLLA MVFLGARGA 
      TSGEMNEIL KLDDMVTFN PHLIFKNVS ESIEVGPES 
      GVAVSAIVR ELYSDRSKG KLLGFYKER VRQFYDGHV 
      EEASFREIG DVIRRRTNL LVKKHTDGR IGEFLKDGS 
      LVAKPPLAG VSVSIFQTD CSHGSTEGR DGELHFVVL 
      PSLRQRRLI PIPATVYRS GFLAGYEPS LDATAAALG 
      SKDATISTI FIIPGQQGV TAPGDGLSR LEKRLVESS 
      FKKGAWSRL LRSLIPRPG LELQIPRFQ HRSLVNATQ 
      SLKRMGLHE LFDAQKADL KGLNGAAHE LYLSDVVQL 
      NEFATCGEG RIGEGHHKE VYPATAERR SLDWEDYQR 
      AFHDPLHDP RYLDLPLAQ RPRQARLPE TPRLRFDRP 
      FLYFVRHNP SGLILHMGR FHPRLLP
    CDS sequence
    • >TC002085 RA
      atgtggcgg ctgctgctt ctcgccctt ctggcggcg 
      gctgaggac aacaaggac aaggacagg acggtgtta 
      gggtccagc gtcgtcact tcagtctcc gtcataatg 
      gacgccgga aacggctct aaaatttac accgatgcg 
      gttggcaaa cctgtatca aaaccacag gttgccaca 
      gatgttgcc agccctatt gaactactc aatccagac 
      agatacgag ttttacaca tttgacgat gccggtgat 
      ttggtcaaa agactaatg acacttgaa gaaatccac 
      gacatcata gcaaccggt gacgatgac gtcacactc 
      gacgccttc atgcccgaa aaacgggtc aacgacgtg 
      gtcaataac gtgcaaaac gtacttaaa gaagaaatc 
      aagcaacac aagaatccg gaaaaaccc gaatttgat 
      acaccggac gtttccgat tcgtggagt atgatttta 
      cccgccatt ttcggcaat tcgggggcg gacattaaa 
      ccggaaccg cccaccagt cacgtgaca cctgatact 
      gtgctagta atggccacg actgagcaa atctccacc 
      actcagaaa gtcaacact ttgcaaacc agtagtact 
      agcactact accactagt actacaagt agtacgccc 
      ttaattaac gtggaaatt atcacaaat tcgaattca 
      acaactgaa agaagtgat aacgtgtca ccagttgtt 
      gaaatcaag ccacaacat gagtcagaa ttcaaaaac 
      aacgtcagg aaagggaca aaaaggccg ttcccggtg 
      aaaagtacg accttcaag cccttaagg aggcccaca 
      aggactacg accttaaca gaggcgccc tctacaaca 
      actaccaca actacaaca cctgcgaca acaacagaa 
      cccgtcagt gagacaata gtaccggat gctggcgcc 
      cagtttgag aaaatcttg cctgaaaca acaacaata 
      acaacagta ccaacaaca acaacaccg ccaacaaca 
      acagaactt gacagaaag acaatagta ccacaattt 
      gagaaaatc gcgcttgaa acaacaaca acaaccgag 
      cctgagaca acctccact gaaatgcca agtaccacc 
      aaagtggta cccgaaaca acaacaaca acaactcca 
      acaactacc caacaagaa acgtccact tatggtacc 
      ttcagtacc tttttcctg gtcagtgac accaagaac 
      cagtcggaa cccgacttg agtaccctt ggtaccgaa 
      tacgtcact gtcaaaaca ctgagtacc aacacaacg 
      gaatcgacg gtacctgac cttgagcag ttcctccta 
      acttcgacc aacatttac gaaattaat tcgcaacta 
      tccgatagt accactgaa gaaaaaacg gaaacaacc 
      gaagcaagt gagaagttt ttgatccgg ctgacgaac 
      ccgaccgaa gtaccgacc accacaacc gatcagacc 
      ttcattgaa acaatcgaa caattgatt tcacaagct 
      gttggtact tctggaccg tacgttgag gcacagaag 
      gaggcttca atggttgag gagatgatg aaaaatggg 
      gggaataaa acaatggag gaggttatg gcggaaacc 
      gccactgca ggttcgagt ctactagat atggtacca 
      acaaaagta gtacccaca acaatggat cttagtgag 
      tcggttgat tcgctccta tcgcaagta ctgcaagta 
      tccacgact ctagaaccc gaaaccaca acagtggta 
      ccaacggtg gtaccaccc gaaagtcat atcatcaat 
      atcaccata atacggaac gaaccaaaa ccggccaaa 
      gccgacttg agtctcgaa atggagtac gacgccatg 
      ttggacgag atgcgcaaa aatggtacc ttcgatgac 
      agtaccatg cccaaaaat ggttctttg gtcgaaacc 
      acaactatt gacagtacc atgccccaa aatggtact 
      ttggtcgaa accacaacc attgacagt accacgact 
      tttgacagt accacgact gaagaaaca atcgagagt 
      agcaccacg ccctcgatt gagatcaca atcaacgaa 
      aagaacgaa tcaggcgcg acctggacg ttagtctcc 
      accctagcc ccgcccacg tccacgccc cctgccgtg 
      gatttggtc ccgaagccg ctgcaaggc ttcggattg 
      gaggacagt accgcccac cttgacacc gacatttac 
      caattcgtg cagttgtgt aacgaattg gcgttcggt 
      ttttggaag agtgtgaca aatgggcta agtacggcc 
      cgtagcgtt gtggtgtcc ccttttgcc gccacgtca 
      ctcctggcc atggtgttc ctgggggcc cggggggcc 
      acctcgggc gaaatgaac gaaatactc aagctggac 
      gatatggtg acgttcaat ccgcatttg attttcaaa 
      aatgtgtca gagtcgatt gaggtgggg cctgaatca 
      ggggtggct gtgtcggct attgtgagg gagttgtac 
      agtgatagg agtaagggg aagttgttg gggttctat 
      aaagagagg gtgcggcag ttttatgat gggcatgtc 
      gaggaggcg agttttagg gagattggg gatgtgatt 
      aggaggagg acgaatctg ttggttaaa aaacataca 
      gatgggagg attggggag tttttgaag gatgggagt 
      ttggtggcg aagccgccc ctggcgggg gtcagtgtt 
      agcattttc cagacggat tgttcccac ggctcgacc 
      gagggccgc gacggcgag ctccacttc gtggtcctc 
      ccctcgctc cgccagcgc cgcctcatc cccatcccc 
      gccaccgtc taccgttcg ggtttcctc gccggttac 
      gagcccagt ctggacgcc accgccgcc gccctcggc 
      tccaaagac gccaccatc tccaccatc ttcataatc 
      ccgggccag cagggcgtt accgccccc ggggacggg 
      ctctcgcgc ctcgagaag cgcctcgtt gagagttcg 
      ttcaagaaa ggggcgtgg tcgcgcctc ctccgctcg 
      ctcatcccc cgccccggc ctcgagctg caaatcccg 
      cgcttccag caccgctca ttggtcaac gccacccaa 
      tcgctgaag cgcatgggc ttgcacgag ctgttcgac 
      gcccagaag gcggacctg aaggggctg aacggcgcc 
      gcccacgaa ctctacctc tccgacgtc gtccaattg 
      aacgagttt gccacgtgc ggcgagggc aggatcggg 
      gaagggcat cacaaggag gtgtatccg gcgacggcg 
      gagaggcgg agcttggat tgggaggac tatcagagg 
      gcgtttcat gacccgttg catgatccc aggtatctg 
      gatttgcca ttggcgcaa aggccgaga caggccagg 
      ttgcctgag acgcctagg ttgaggttt gatcggccg 
      tttttgtac tttgtgagg cataatccg agtgggctt 
      atcttacat atggggagg ttccatccg aggttattg 
      ccatga
    mRNA sequence
    • >TC002085 RA
      tccgagaag tgtgcatca aagcaagcg agaacagca 
      gaacgattg cggtggact tgaaacaca ctgaggaag 
      catgtggcg gctgctgct tctcgccct tctggcggc 
      ggctgagga caacaagga caaggacag gacggtgtt 
      agggtccag cgtcgtcac ttcagtctc cgtcataat 
      ggacgccgg aaacggctc taaaattta caccgatgc 
      ggttggcaa acctgtatc aaaaccaca ggttgccac 
      agatgttgc cagccctat tgaactact caatccaga 
      cagatacga gttttacac atttgacga tgccggtga 
      tttggtcaa aagactaat gacacttga agaaatcca 
      cgacatcat agcaaccgg tgacgatga cgtcacact 
      cgacgcctt catgcccga aaaacgggt caacgacgt 
      ggtcaataa cgtgcaaaa cgtacttaa agaagaaat 
      caagcaaca caagaatcc ggaaaaacc cgaatttga 
      tacaccgga cgtttccga ttcgtggag tatgatttt 
      acccgccat tttcggcaa ttcgggggc ggacattaa 
      accggaacc gcccaccag tcacgtgac acctgatac 
      tgtgctagt aatggccac gactgagca aatctccac 
      cactcagaa agtcaacac tttgcaaac cagtagtac 
      tagcactac taccactag tactacaag tagtacgcc 
      cttaattaa cgtggaaat tatcacaaa ttcgaattc 
      aacaactga aagaagtga taacgtgtc accagttgt 
      tgaaatcaa gccacaaca tgagtcaga attcaaaaa 
      caacgtcag gaaagggac aaaaaggcc gttcccggt 
      gaaaagtac gaccttcaa gcccttaag gaggcccac 
      aaggactac gaccttaac agaggcgcc ctctacaac 
      aactaccac aactacaac acctgcgac aacaacaga 
      acccgtcag tgagacaat agtaccgga tgctggcgc 
      ccagtttga gaaaatctt gcctgaaac aacaacaat 
      aacaacagt accaacaac aacaacacc gccaacaac 
      aacagaact tgacagaaa gacaatagt accacaatt 
      tgagaaaat cgcgcttga aacaacaac aacaaccga 
      gcctgagac aacctccac tgaaatgcc aagtaccac 
      caaagtggt acccgaaac aacaacaac aacaactcc 
      aacaactac ccaacaaga aacgtccac ttatggtac 
      cttcagtac ctttttcct ggtcagtga caccaagaa 
      ccagtcgga acccgactt gagtaccct tggtaccga 
      atacgtcac tgtcaaaac actgagtac caacacaac 
      ggaatcgac ggtacctga ccttgagca gttcctcct 
      aacttcgac caacattta cgaaattaa ttcgcaact 
      atccgatag taccactga agaaaaaac ggaaacaac 
      cgaagcaag tgagaagtt tttgatccg gctgacgaa 
      cccgaccga agtaccgac caccacaac cgatcagac 
      cttcattga aacaatcga acaattgat ttcacaagc 
      tgttggtac ttctggacc gtacgttga ggcacagaa 
      ggaggcttc aatggttga ggagatgat gaaaaatgg 
      ggggaataa aacaatgga ggaggttat ggcggaaac 
      cgccactgc aggttcgag tctactaga tatggtacc 
      aacaaaagt agtacccac aacaatgga tcttagtga 
      gtcggttga ttcgctcct atcgcaagt actgcaagt 
      atccacgac tctagaacc cgaaaccac aacagtggt 
      accaacggt ggtaccacc cgaaagtca tatcatcaa 
      tatcaccat aatacggaa cgaaccaaa accggccaa 
      agccgactt gagtctcga aatggagta cgacgccat 
      gttggacga gatgcgcaa aaatggtac cttcgatga 
      cagtaccat gcccaaaaa tggttcttt ggtcgaaac 
      cacaactat tgacagtac catgcccca aaatggtac 
      tttggtcga aaccacaac cattgacag taccacgac 
      ttttgacag taccacgac tgaagaaac aatcgagag 
      tagcaccac gccctcgat tgagatcac aatcaacga 
      aaagaacga atcaggcgc gacctggac gttagtctc 
      caccctagc cccgcccac gtccacgcc ccctgccgt 
      ggatttggt cccgaagcc gctgcaagg cttcggatt 
      ggaggacag taccgccca ccttgacac cgacattta 
      ccaattcgt gcagttgtg taacgaatt ggcgttcgg 
      tttttggaa gagtgtgac aaatgggct aagtacggc 
      ccgtagcgt tgtggtgtc cccttttgc cgccacgtc 
      actcctggc catggtgtt cctgggggc ccggggggc 
      cacctcggg cgaaatgaa cgaaatact caagctgga 
      cgatatggt gacgttcaa tccgcattt gattttcaa 
      aaatgtgtc agagtcgat tgaggtggg gcctgaatc 
      aggggtggc tgtgtcggc tattgtgag ggagttgta 
      cagtgatag gagtaaggg gaagttgtt ggggttcta 
      taaagagag ggtgcggca gttttatga tgggcatgt 
      cgaggaggc gagttttag ggagattgg ggatgtgat 
      taggaggag gacgaatct gttggttaa aaaacatac 
      agatgggag gattgggga gtttttgaa ggatgggag 
      tttggtggc gaagccgcc cctggcggg ggtcagtgt 
      tagcatttt ccagacgga ttgttccca cggctcgac 
      cgagggccg cgacggcga gctccactt cgtggtcct 
      cccctcgct ccgccagcg ccgcctcat ccccatccc 
      cgccaccgt ctaccgttc gggtttcct cgccggtta 
      cgagcccag tctggacgc caccgccgc cgccctcgg 
      ctccaaaga cgccaccat ctccaccat cttcataat 
      cccgggcca gcagggcgt taccgcccc cggggacgg 
      gctctcgcg cctcgagaa gcgcctcgt tgagagttc 
      gttcaagaa aggggcgtg gtcgcgcct cctccgctc 
      gctcatccc ccgccccgg cctcgagct gcaaatccc 
      gcgcttcca gcaccgctc attggtcaa cgccaccca 
      atcgctgaa gcgcatggg cttgcacga gctgttcga 
      cgcccagaa ggcggacct gaaggggct gaacggcgc 
      cgcccacga actctacct ctccgacgt cgtccaatt 
      gaacgagtt tgccacgtg cggcgaggg caggatcgg 
      ggaagggca tcacaagga ggtgtatcc ggcgacggc 
      ggagaggcg gagcttgga ttgggagga ctatcagag 
      ggcgtttca tgacccgtt gcatgatcc caggtatct 
      ggatttgcc attggcgca aaggccgag acaggccag 
      gttgcctga gacgcctag gttgaggtt tgatcggcc 
      gtttttgta ctttgtgag gcataatcc gagtgggct 
      tatcttaca tatggggag gttccatcc gaggttatt 
      gccatgagg gggaggggc gggggtgta tatagtttg 
      agatggaac tatcggact gagatggat agttgcatg 
      gcgaaggct tttattcac ttaatttat tgtaaattt 
      agacaattt gtgtgtaaa gttggattc ttaaattat 
      tttagtgca atttttgta ttattttat taaggatta 
      caaataaac aaatgactg tttc