| General information | |||
| TC002085 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007425.3: 6028977..6039966 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC002085 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_03300 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
egg chamber number decreased - Number of animals showing the phenotype: 4
egg chamber mostly not present - Number of animals showing the phenotype: 4
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC002085 RA
MWRLLLLAL LAAAEDNKD KDRTVLGSS VVTSVSVIM DAGNGSKIY TDAVGKPVS KPQVATDVA SPIELLNPD RYEFYTFDD AGDLVKRLM TLEEIHDII ATGDDDVTL DAFMPEKRV NDVVNNVQN VLKEEIKQH KNPEKPEFD TPDVSDSWS MILPAIFGN SGADIKPEP PTSHVTPDT VLVMATTEQ ISTTQKVNT LQTSSTSTT TTSTTSSTP LINVEIITN SNSTTERSD NVSPVVEIK PQHESEFKN NVRKGTKRP FPVKSTTFK PLRRPTRTT TLTEAPSTT TTTTTTPAT TTEPVSETI VPDAGAQFE KILPETTTI TTVPTTTTP PTTTELDRK TIVPQFEKI ALETTTTTE PETTSTEMP STTKVVPET TTTTTPTTT QQETSTYGT FSTFFLVSD TKNQSEPDL STLGTEYVT VKTLSTNTT ESTVPDLEQ FLLTSTNIY EINSQLSDS TTEEKTETT EASEKFLIR LTNPTEVPT TTTDQTFIE TIEQLISQA VGTSGPYVE AQKEASMVE EMMKNGGNK TMEEVMAET ATAGSSLLD MVPTKVVPT TMDLSESVD SLLSQVLQV STTLEPETT TVVPTVVPP ESHIINITI IRNEPKPAK ADLSLEMEY DAMLDEMRK NGTFDDSTM PKNGSLVET TTIDSTMPQ NGTLVETTT IDSTTTFDS TTTEETIES STTPSIEIT INEKNESGA TWTLVSTLA PPTSTPPAV DLVPKPLQG FGLEDSTAH LDTDIYQFV QLCNELAFG FWKSVTNGL STARSVVVS PFAATSLLA MVFLGARGA TSGEMNEIL KLDDMVTFN PHLIFKNVS ESIEVGPES GVAVSAIVR ELYSDRSKG KLLGFYKER VRQFYDGHV EEASFREIG DVIRRRTNL LVKKHTDGR IGEFLKDGS LVAKPPLAG VSVSIFQTD CSHGSTEGR DGELHFVVL PSLRQRRLI PIPATVYRS GFLAGYEPS LDATAAALG SKDATISTI FIIPGQQGV TAPGDGLSR LEKRLVESS FKKGAWSRL LRSLIPRPG LELQIPRFQ HRSLVNATQ SLKRMGLHE LFDAQKADL KGLNGAAHE LYLSDVVQL NEFATCGEG RIGEGHHKE VYPATAERR SLDWEDYQR AFHDPLHDP RYLDLPLAQ RPRQARLPE TPRLRFDRP FLYFVRHNP SGLILHMGR FHPRLLP
- >TC002085 RA
atgtggcgg ctgctgctt ctcgccctt ctggcggcg gctgaggac aacaaggac aaggacagg acggtgtta gggtccagc gtcgtcact tcagtctcc gtcataatg gacgccgga aacggctct aaaatttac accgatgcg gttggcaaa cctgtatca aaaccacag gttgccaca gatgttgcc agccctatt gaactactc aatccagac agatacgag ttttacaca tttgacgat gccggtgat ttggtcaaa agactaatg acacttgaa gaaatccac gacatcata gcaaccggt gacgatgac gtcacactc gacgccttc atgcccgaa aaacgggtc aacgacgtg gtcaataac gtgcaaaac gtacttaaa gaagaaatc aagcaacac aagaatccg gaaaaaccc gaatttgat acaccggac gtttccgat tcgtggagt atgatttta cccgccatt ttcggcaat tcgggggcg gacattaaa ccggaaccg cccaccagt cacgtgaca cctgatact gtgctagta atggccacg actgagcaa atctccacc actcagaaa gtcaacact ttgcaaacc agtagtact agcactact accactagt actacaagt agtacgccc ttaattaac gtggaaatt atcacaaat tcgaattca acaactgaa agaagtgat aacgtgtca ccagttgtt gaaatcaag ccacaacat gagtcagaa ttcaaaaac aacgtcagg aaagggaca aaaaggccg ttcccggtg aaaagtacg accttcaag cccttaagg aggcccaca aggactacg accttaaca gaggcgccc tctacaaca actaccaca actacaaca cctgcgaca acaacagaa cccgtcagt gagacaata gtaccggat gctggcgcc cagtttgag aaaatcttg cctgaaaca acaacaata acaacagta ccaacaaca acaacaccg ccaacaaca acagaactt gacagaaag acaatagta ccacaattt gagaaaatc gcgcttgaa acaacaaca acaaccgag cctgagaca acctccact gaaatgcca agtaccacc aaagtggta cccgaaaca acaacaaca acaactcca acaactacc caacaagaa acgtccact tatggtacc ttcagtacc tttttcctg gtcagtgac accaagaac cagtcggaa cccgacttg agtaccctt ggtaccgaa tacgtcact gtcaaaaca ctgagtacc aacacaacg gaatcgacg gtacctgac cttgagcag ttcctccta acttcgacc aacatttac gaaattaat tcgcaacta tccgatagt accactgaa gaaaaaacg gaaacaacc gaagcaagt gagaagttt ttgatccgg ctgacgaac ccgaccgaa gtaccgacc accacaacc gatcagacc ttcattgaa acaatcgaa caattgatt tcacaagct gttggtact tctggaccg tacgttgag gcacagaag gaggcttca atggttgag gagatgatg aaaaatggg gggaataaa acaatggag gaggttatg gcggaaacc gccactgca ggttcgagt ctactagat atggtacca acaaaagta gtacccaca acaatggat cttagtgag tcggttgat tcgctccta tcgcaagta ctgcaagta tccacgact ctagaaccc gaaaccaca acagtggta ccaacggtg gtaccaccc gaaagtcat atcatcaat atcaccata atacggaac gaaccaaaa ccggccaaa gccgacttg agtctcgaa atggagtac gacgccatg ttggacgag atgcgcaaa aatggtacc ttcgatgac agtaccatg cccaaaaat ggttctttg gtcgaaacc acaactatt gacagtacc atgccccaa aatggtact ttggtcgaa accacaacc attgacagt accacgact tttgacagt accacgact gaagaaaca atcgagagt agcaccacg ccctcgatt gagatcaca atcaacgaa aagaacgaa tcaggcgcg acctggacg ttagtctcc accctagcc ccgcccacg tccacgccc cctgccgtg gatttggtc ccgaagccg ctgcaaggc ttcggattg gaggacagt accgcccac cttgacacc gacatttac caattcgtg cagttgtgt aacgaattg gcgttcggt ttttggaag agtgtgaca aatgggcta agtacggcc cgtagcgtt gtggtgtcc ccttttgcc gccacgtca ctcctggcc atggtgttc ctgggggcc cggggggcc acctcgggc gaaatgaac gaaatactc aagctggac gatatggtg acgttcaat ccgcatttg attttcaaa aatgtgtca gagtcgatt gaggtgggg cctgaatca ggggtggct gtgtcggct attgtgagg gagttgtac agtgatagg agtaagggg aagttgttg gggttctat aaagagagg gtgcggcag ttttatgat gggcatgtc gaggaggcg agttttagg gagattggg gatgtgatt aggaggagg acgaatctg ttggttaaa aaacataca gatgggagg attggggag tttttgaag gatgggagt ttggtggcg aagccgccc ctggcgggg gtcagtgtt agcattttc cagacggat tgttcccac ggctcgacc gagggccgc gacggcgag ctccacttc gtggtcctc ccctcgctc cgccagcgc cgcctcatc cccatcccc gccaccgtc taccgttcg ggtttcctc gccggttac gagcccagt ctggacgcc accgccgcc gccctcggc tccaaagac gccaccatc tccaccatc ttcataatc ccgggccag cagggcgtt accgccccc ggggacggg ctctcgcgc ctcgagaag cgcctcgtt gagagttcg ttcaagaaa ggggcgtgg tcgcgcctc ctccgctcg ctcatcccc cgccccggc ctcgagctg caaatcccg cgcttccag caccgctca ttggtcaac gccacccaa tcgctgaag cgcatgggc ttgcacgag ctgttcgac gcccagaag gcggacctg aaggggctg aacggcgcc gcccacgaa ctctacctc tccgacgtc gtccaattg aacgagttt gccacgtgc ggcgagggc aggatcggg gaagggcat cacaaggag gtgtatccg gcgacggcg gagaggcgg agcttggat tgggaggac tatcagagg gcgtttcat gacccgttg catgatccc aggtatctg gatttgcca ttggcgcaa aggccgaga caggccagg ttgcctgag acgcctagg ttgaggttt gatcggccg tttttgtac tttgtgagg cataatccg agtgggctt atcttacat atggggagg ttccatccg aggttattg ccatga
- >TC002085 RA
tccgagaag tgtgcatca aagcaagcg agaacagca gaacgattg cggtggact tgaaacaca ctgaggaag catgtggcg gctgctgct tctcgccct tctggcggc ggctgagga caacaagga caaggacag gacggtgtt agggtccag cgtcgtcac ttcagtctc cgtcataat ggacgccgg aaacggctc taaaattta caccgatgc ggttggcaa acctgtatc aaaaccaca ggttgccac agatgttgc cagccctat tgaactact caatccaga cagatacga gttttacac atttgacga tgccggtga tttggtcaa aagactaat gacacttga agaaatcca cgacatcat agcaaccgg tgacgatga cgtcacact cgacgcctt catgcccga aaaacgggt caacgacgt ggtcaataa cgtgcaaaa cgtacttaa agaagaaat caagcaaca caagaatcc ggaaaaacc cgaatttga tacaccgga cgtttccga ttcgtggag tatgatttt acccgccat tttcggcaa ttcgggggc ggacattaa accggaacc gcccaccag tcacgtgac acctgatac tgtgctagt aatggccac gactgagca aatctccac cactcagaa agtcaacac tttgcaaac cagtagtac tagcactac taccactag tactacaag tagtacgcc cttaattaa cgtggaaat tatcacaaa ttcgaattc aacaactga aagaagtga taacgtgtc accagttgt tgaaatcaa gccacaaca tgagtcaga attcaaaaa caacgtcag gaaagggac aaaaaggcc gttcccggt gaaaagtac gaccttcaa gcccttaag gaggcccac aaggactac gaccttaac agaggcgcc ctctacaac aactaccac aactacaac acctgcgac aacaacaga acccgtcag tgagacaat agtaccgga tgctggcgc ccagtttga gaaaatctt gcctgaaac aacaacaat aacaacagt accaacaac aacaacacc gccaacaac aacagaact tgacagaaa gacaatagt accacaatt tgagaaaat cgcgcttga aacaacaac aacaaccga gcctgagac aacctccac tgaaatgcc aagtaccac caaagtggt acccgaaac aacaacaac aacaactcc aacaactac ccaacaaga aacgtccac ttatggtac cttcagtac ctttttcct ggtcagtga caccaagaa ccagtcgga acccgactt gagtaccct tggtaccga atacgtcac tgtcaaaac actgagtac caacacaac ggaatcgac ggtacctga ccttgagca gttcctcct aacttcgac caacattta cgaaattaa ttcgcaact atccgatag taccactga agaaaaaac ggaaacaac cgaagcaag tgagaagtt tttgatccg gctgacgaa cccgaccga agtaccgac caccacaac cgatcagac cttcattga aacaatcga acaattgat ttcacaagc tgttggtac ttctggacc gtacgttga ggcacagaa ggaggcttc aatggttga ggagatgat gaaaaatgg ggggaataa aacaatgga ggaggttat ggcggaaac cgccactgc aggttcgag tctactaga tatggtacc aacaaaagt agtacccac aacaatgga tcttagtga gtcggttga ttcgctcct atcgcaagt actgcaagt atccacgac tctagaacc cgaaaccac aacagtggt accaacggt ggtaccacc cgaaagtca tatcatcaa tatcaccat aatacggaa cgaaccaaa accggccaa agccgactt gagtctcga aatggagta cgacgccat gttggacga gatgcgcaa aaatggtac cttcgatga cagtaccat gcccaaaaa tggttcttt ggtcgaaac cacaactat tgacagtac catgcccca aaatggtac tttggtcga aaccacaac cattgacag taccacgac ttttgacag taccacgac tgaagaaac aatcgagag tagcaccac gccctcgat tgagatcac aatcaacga aaagaacga atcaggcgc gacctggac gttagtctc caccctagc cccgcccac gtccacgcc ccctgccgt ggatttggt cccgaagcc gctgcaagg cttcggatt ggaggacag taccgccca ccttgacac cgacattta ccaattcgt gcagttgtg taacgaatt ggcgttcgg tttttggaa gagtgtgac aaatgggct aagtacggc ccgtagcgt tgtggtgtc cccttttgc cgccacgtc actcctggc catggtgtt cctgggggc ccggggggc cacctcggg cgaaatgaa cgaaatact caagctgga cgatatggt gacgttcaa tccgcattt gattttcaa aaatgtgtc agagtcgat tgaggtggg gcctgaatc aggggtggc tgtgtcggc tattgtgag ggagttgta cagtgatag gagtaaggg gaagttgtt ggggttcta taaagagag ggtgcggca gttttatga tgggcatgt cgaggaggc gagttttag ggagattgg ggatgtgat taggaggag gacgaatct gttggttaa aaaacatac agatgggag gattgggga gtttttgaa ggatgggag tttggtggc gaagccgcc cctggcggg ggtcagtgt tagcatttt ccagacgga ttgttccca cggctcgac cgagggccg cgacggcga gctccactt cgtggtcct cccctcgct ccgccagcg ccgcctcat ccccatccc cgccaccgt ctaccgttc gggtttcct cgccggtta cgagcccag tctggacgc caccgccgc cgccctcgg ctccaaaga cgccaccat ctccaccat cttcataat cccgggcca gcagggcgt taccgcccc cggggacgg gctctcgcg cctcgagaa gcgcctcgt tgagagttc gttcaagaa aggggcgtg gtcgcgcct cctccgctc gctcatccc ccgccccgg cctcgagct gcaaatccc gcgcttcca gcaccgctc attggtcaa cgccaccca atcgctgaa gcgcatggg cttgcacga gctgttcga cgcccagaa ggcggacct gaaggggct gaacggcgc cgcccacga actctacct ctccgacgt cgtccaatt gaacgagtt tgccacgtg cggcgaggg caggatcgg ggaagggca tcacaagga ggtgtatcc ggcgacggc ggagaggcg gagcttgga ttgggagga ctatcagag ggcgtttca tgacccgtt gcatgatcc caggtatct ggatttgcc attggcgca aaggccgag acaggccag gttgcctga gacgcctag gttgaggtt tgatcggcc gtttttgta ctttgtgag gcataatcc gagtgggct tatcttaca tatggggag gttccatcc gaggttatt gccatgagg gggaggggc gggggtgta tatagtttg agatggaac tatcggact gagatggat agttgcatg gcgaaggct tttattcac ttaatttat tgtaaattt agacaattt gtgtgtaaa gttggattc ttaaattat tttagtgca atttttgta ttattttat taaggatta caaataaac aaatgactg tttc
