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Gene information
General information
TC002084 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid75: 1769022..1769982
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002084
    iBeetle screen iB_03299
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002084 RA
      MTSLRSRMR YMNPYDIHK AIINEYVLK 
      QPGDTKLLQ RDASKDRTD IDVLKANHR 
      FLWDDSIPD TWEAQFAKK YYDKLFKEY 
      CIADLTYYK ENKIALRWR IEREVIEGK 
      GQFTCGNKK CPERENLRT WEVNFAYKE 
      QGEKKNALV KIRLCPDCS LKLNHKSKK 
      REVKRLKSK KKKKVKKRE KGDEEGGEE 
      GGTSQPKSD SEPETSESP WDQHKPEEI 
      KTRDEEMED YLQDLLL
    CDS sequence
    • >TC002084 RA
      atgacgtct ctccgctca cggatgcgt tacatgaac 
      ccttacgac atccacaag gccattatc aacgaatac 
      gtgctaaaa cagcccgga gacaccaaa ttattacaa 
      cgtgatgcg tcaaaagac cgcaccgat atcgacgtc 
      cttaaagcc aaccatagg ttcctgtgg gacgactca 
      atccccgat acatgggaa gcccaattc gccaaaaaa 
      tattacgac aagcttttc aaagagtat tgcatcgca 
      gacctcact tattacaag gaaaataag atcgccctg 
      cggtggcgc atcgaacgc gaggtgatc gaaggcaag 
      ggtcagttc acttgtggt aataaaaag tgccccgaa 
      agggagaat ttaagaacg tgggaggta aactttgct 
      tataaagaa caaggcgag aagaagaat gctctagtg 
      aagattagg ctgtgcccc gattgttcg cttaagttg 
      aaccacaag agtaaaaaa cgcgaagtt aaaagactg 
      aagagtaaa aagaagaag aaagtgaag aaacgggaa 
      aagggtgac gaagaggga ggggaagaa gggggcaca 
      agtcagccg aagtcagat tcggaacct gagacaagt 
      gagtcgcct tgggatcaa cacaagccg gaagaaatc 
      aagacacgg gatgaagaa atggaagat tatcttcaa 
      gacttgctt ttgtaa
    mRNA sequence
    • >TC002084 RA
      cgtgtaatt aattttcgc tgggcctgt tacaggtcg 
      ctgtaatag cgacatgtc agtgtcaca attgtggtc 
      acaattttg ggttatgtt gtgacagcc ccccgaaat 
      gacgtctct ccgctcacg gatgcgtta catgaaccc 
      ttacgacat ccacaaggc cattatcaa cgaatacgt 
      gctaaaaca gcccggaga caccaaatt attacaacg 
      tgatgcgtc aaaagaccg caccgatat cgacgtcct 
      taaagccaa ccataggtt cctgtggga cgactcaat 
      ccccgatac atgggaagc ccaattcgc caaaaaata 
      ttacgacaa gcttttcaa agagtattg catcgcaga 
      cctcactta ttacaagga aaataagat cgccctgcg 
      gtggcgcat cgaacgcga ggtgatcga aggcaaggg 
      tcagttcac ttgtggtaa taaaaagtg ccccgaaag 
      ggagaattt aagaacgtg ggaggtaaa ctttgctta 
      taaagaaca aggcgagaa gaagaatgc tctagtgaa 
      gattaggct gtgccccga ttgttcgct taagttgaa 
      ccacaagag taaaaaacg cgaagttaa aagactgaa 
      gagtaaaaa gaagaagaa agtgaagaa acgggaaaa 
      gggtgacga agagggagg ggaagaagg gggcacaag 
      tcagccgaa gtcagattc ggaacctga gacaagtga 
      gtcgccttg ggatcaaca caagccgga agaaatcaa 
      gacacggga tgaagaaat ggaagatta tcttcaaga 
      cttgctttt gtaaaaacc aattgtttg tctgtattt 
      atttgtaaa gtaattaaa taaatgaaa atttgaaca