Status
Please wait ...
Gene information
General information
TC002083 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 6080989..6087103
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002083
    iBeetle screen iB_03298

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002083 RA
      MPQLTPEMM RSRAYSSGA KLLPHLDLR RCDRSENDL 
      ESFTGGMPT LDYSWIDKR ELDHRIFVN RSLHLENIK 
      FYGFDMDYT LAEYKSPEY EKLGFDLVK ERMVSIGYP 
      QEILEFEYD PTFAVRGLW FDAQYGNLL KVDAYGNIL 
      VCVHGFEFI KHSQVYELY PNKFLPLDE SRVYVLNTL 
      FNLPETYLL ACLVDFFTN SPQYTKTQT GVKFGDLYM 
      SFKSIFQDV RNAVDWVHL QGDLKQKTI ENLEKYVKK 
      DERLPMFLT RLRESGAKT FLLTNSDYN FTDNIMTYL 
      FDFPHGAKP GDPHRNWKT YFDTIVVDA RKPLFFGEG 
      TILRQVDTA TGALRVGIH TGPFQKEHV YSGGSCDVF 
      TKLIGAKGK DVLYVGDHI FGDILKSKK IRGWRTFLI 
      VPELVRELH VWTDKCQFF DELQMLDVK LGEMYKNLD 
      SSTKEKPDI SKLRAAIRD VTHKMDMSY GMMGSLFRS 
      GSRQTFFSS QVVRYADLY AATHLNLLY YPFSYMFRA 
      PAMLLPHES TVAHEQRFV LEARDRENE ERKPPKVLE 
      RTHSQIPHV RPETPLSVT HNHDEDFSD EDSDDKISA 
      KNGEKTQ
    CDS sequence
    • >TC002083 RA
      atgcctcaa ttaacgccg gaaatgatg cgatcgcgg 
      gcatattcc agtggtgcc aagttgctt ccgcacctg 
      gatttgcga cgttgcgac aggtccgaa aacgattta 
      gaaagtttt accggcggc atgcccacg ctggactat 
      agttggatt gacaagaga gagttggat cacaggatt 
      tttgtcaac cgtagtttg cacttggaa aacattaag 
      ttttacggt tttgacatg gactacacc ctcgctgag 
      tacaaatcg cccgaatat gaaaaactg ggctttgac 
      cttgttaaa gaacgtatg gtatccatt ggttaccca 
      caggaaatc ctcgaattc gaatatgac ccaactttc 
      gcagttcga ggtttatgg tttgatgct caatatggc 
      aacttgtta aaagttgac gcttatgga aatattcta 
      gtgtgcgtg cacggcttt gaatttatt aaacactca 
      caagtatac gaattgtat ccaaacaag tttcttcca 
      ttggatgaa tcaagagta tacgtttta aacacactt 
      tttaacctc ccagaaact tatctttta gcctgtctt 
      gtagatttt ttcacgaat tcgccacaa tacaccaag 
      acgcagacc ggtgtcaag tttggcgac ttgtacatg 
      tcgttcaag tccattttc caggatgtg cgtaacgcc 
      gtcgattgg gtgcacctc cagggcgac ttgaagcaa 
      aaaacaatc gagaatctc gaaaaatac gtgaaaaaa 
      gacgaacgt cttcctatg tttctaacg cgtttgaga 
      gagagcggt gctaaaacg ttccttctc acaaatagc 
      gattacaac tttaccgac aatatcatg acctacttg 
      ttcgatttt ccgcatggg gcgaagccc ggggacccc 
      caccgtaac tggaagact tacttcgac acgattgtt 
      gtggacgcc cggaagccc cttttcttc ggcgagggg 
      actattttg aggcaggtg gacacggcc acgggggcc 
      ctccgggtg ggcatccac acggggccc ttccagaag 
      gagcacgtg tactcgggg ggctcctgt gacgtcttc 
      accaagctc attggggcc aagggcaag gacgtgctc 
      tatgtgggt gaccacatt tttggggat attttgaaa 
      agtaagaaa attcgaggt tggcgaacc ttcctcatt 
      gtgcctgaa ctagtgagg gagttgcac gtttggact 
      gacaaatgt cagtttttc gatgagttg caaatgtta 
      gacgtgaag ttaggcgaa atgtataag aatctagac 
      agtagcacg aaggaaaaa ccggatatt tccaaacta 
      agggccgcc atacgtgac gtcacgcat aaaatggac 
      atgtcgtac ggtatgatg gggagtctt ttcaggtca 
      gggtcaagg caaacgttc ttctcgtca caggtggtg 
      agatacgcc gatttgtac gccgccacg cacctcaac 
      ctcctctac taccccttc tcgtacatg ttccgggcc 
      cccgccatg ctcctgccg cacgagtcg acagtggcg 
      cacgagcaa cgtttcgtt ttggaggcc cgagaccgc 
      gaaaacgag gaacggaag ccccccaaa gtgctcgaa 
      cgcactcac agccaaatc ccgcacgtg cgacctgag 
      acacctttg agtgtcact cacaatcat gacgaggat 
      ttctctgat gaggacagc gacgacaag atttccgcc 
      aaaaacggc gaaaaaaca caataa
    mRNA sequence
    • >TC002083 RA
      tcgtaaaca acacgcgtc tttatcatg accccagcg 
      agcgccagt tcagttcag ttgttggtt gtctttcgt 
      tcgataaaa tgcctcaat taacgccgg aaatgatgc 
      gatcgcggg catattcca gtggtgcca agttgcttc 
      cgcacctgg atttgcgac gttgcgaca ggtccgaaa 
      acgatttag aaagtttta ccggcggca tgcccacgc 
      tggactata gttggattg acaagagag agttggatc 
      acaggattt ttgtcaacc gtagtttgc acttggaaa 
      acattaagt tttacggtt ttgacatgg actacaccc 
      tcgctgagt acaaatcgc ccgaatatg aaaaactgg 
      gctttgacc ttgttaaag aacgtatgg tatccattg 
      gttacccac aggaaatcc tcgaattcg aatatgacc 
      caactttcg cagttcgag gtttatggt ttgatgctc 
      aatatggca acttgttaa aagttgacg cttatggaa 
      atattctag tgtgcgtgc acggctttg aatttatta 
      aacactcac aagtatacg aattgtatc caaacaagt 
      ttcttccat tggatgaat caagagtat acgttttaa 
      acacacttt ttaacctcc cagaaactt atcttttag 
      cctgtcttg tagattttt tcacgaatt cgccacaat 
      acaccaaga cgcagaccg gtgtcaagt ttggcgact 
      tgtacatgt cgttcaagt ccattttcc aggatgtgc 
      gtaacgccg tcgattggg tgcacctcc agggcgact 
      tgaagcaaa aaacaatcg agaatctcg aaaaatacg 
      tgaaaaaag acgaacgtc ttcctatgt ttctaacgc 
      gtttgagag agagcggtg ctaaaacgt tccttctca 
      caaatagcg attacaact ttaccgaca atatcatga 
      cctacttgt tcgattttc cgcatgggg cgaagcccg 
      gggaccccc accgtaact ggaagactt acttcgaca 
      cgattgttg tggacgccc ggaagcccc ttttcttcg 
      gcgagggga ctattttga ggcaggtgg acacggcca 
      cgggggccc tccgggtgg gcatccaca cggggccct 
      tccagaagg agcacgtgt actcggggg gctcctgtg 
      acgtcttca ccaagctca ttggggcca agggcaagg 
      acgtgctct atgtgggtg accacattt ttggggata 
      ttttgaaaa gtaagaaaa ttcgaggtt ggcgaacct 
      tcctcattg tgcctgaac tagtgaggg agttgcacg 
      tttggactg acaaatgtc agtttttcg atgagttgc 
      aaatgttag acgtgaagt taggcgaaa tgtataaga 
      atctagaca gtagcacga aggaaaaac cggatattt 
      ccaaactaa gggccgcca tacgtgacg tcacgcata 
      aaatggaca tgtcgtacg gtatgatgg ggagtcttt 
      tcaggtcag ggtcaaggc aaacgttct tctcgtcac 
      aggtggtga gatacgccg atttgtacg ccgccacgc 
      acctcaacc tcctctact accccttct cgtacatgt 
      tccgggccc ccgccatgc tcctgccgc acgagtcga 
      cagtggcgc acgagcaac gtttcgttt tggaggccc 
      gagaccgcg aaaacgagg aacggaagc cccccaaag 
      tgctcgaac gcactcaca gccaaatcc cgcacgtgc 
      gacctgaga cacctttga gtgtcactc acaatcatg 
      acgaggatt tctctgatg aggacagcg acgacaaga 
      tttccgcca aaaacggcg aaaaaacac aataaaagc 
      aagcgcaca gttttctag aaattattg ctcattttt 
      tacgttgtt attgagatt ttatatccg tagtgttgg 
      ctaacctgt tttttatta tagtaagtt attgtgttt 
      atttattgt gctttggac acaaattac aaataaaat 
      gacaaagaa aca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY